P33772 (AMIA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylmuramoyl-L-alanine amidase AmiA
EC=3.5.1.28

Gene names

Name:	amiA
Ordered Locus Names:	STM2450

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell-wall hydrolase involved in septum cleavage during cell division By similarity.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Periplasm
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Tat-type signal Potential
	Chain	35 – 289	255	N-acetylmuramoyl-L-alanine amidase AmiA		PRO_0000006461

Sequences

	Sequence		Length	Mass (Da)
	P33772 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 8FCC21089D56F671	FASTA	289	31,659
	10 20 30 40 50 60 MSTFKLLKTL TSRRQVLKTG LAALTLSGMS HAVAKEETLK TSNGHSKPKT KKTGSKRLVM 70 80 90 100 110 120 LDPGHGGIDT GAIGRNGSQE KHVVLAIAKN VRAILRNHGI DARLTRTGDT FIPLYDRVEI 130 140 150 160 170 180 AHKHGADLFM SIHADGFTNP KAAGASVFAL SNRGASSAMA KYLSERENRA DEVAGKKATD 190 200 210 220 230 240 RDHLLQQVLF DLVQTDTIKN SLTLGSHILK KIKPIHKLHS RTTEQAAFVV LKSPSIPSVL 250 260 270 280 VETSFITNPE EERLLGTTAF RQKIATAIAN GIISYFHWFD NQKAHTKKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An oxygen-dependent coproporphyrinogen oxidase encoded by the hemF gene of Salmonella typhimurium." Xu K., Elliott T. J. Bacteriol. 175:4990-4999(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19503 Genomic DNA. Translation: AAA27138.1. AE006468 Genomic DNA. Translation: AAL21344.1.
PIR	A53302.
RefSeq	NP_461385.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P33772.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM2450.
Proteomic databases
PaxDb	P33772.
PRIDE	P33772.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL21344; AAL21344; STM2450.
GeneID	1253972.
KEGG	stm:STM2450.
PATRIC	32383531. VBISalEnt20916_2588.
Phylogenomic databases
eggNOG	COG0860.
HOGENOM	HOG000263827.
KO	K01448.
OMA	RIVMLDP.
ProtClustDB	PRK10319.
Family and domain databases
Gene3D	3.40.630.40. 2 hits.
InterPro	IPR002508. CW_Hdrlase/autolysin_cat. [Graphical view]
Pfam	PF01520. Amidase_3. 1 hit. [Graphical view]
SMART	SM00646. Ami_3. 1 hit. [Graphical view]
PROSITE	PS51318. TAT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMIA_SALTY

Accession

Primary (citable) accession number: P33772

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program