Reviewed, UniProtKB/Swiss-Prot P33772 (AMIA_SALTY)

Names and origin

Protein names

Recommended name:
    Probable N-acetylmuramoyl-L-alanine amidase amiA
    EC=3.5.1.28

Gene names

Name:	amiA
Ordered Locus Names:	STM2450

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted Potential.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Tat-type signal Potential
	Chain	35 – 289	255	Probable N-acetylmuramoyl-L-alanine amidase amiA		PRO_0000006461

Sequences

	Sequence		Length	Mass (Da)
	P33772-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 8FCC21089D56F671	FASTA	289	31,659
	10 20 30 40 50 60 MSTFKLLKTL TSRRQVLKTG LAALTLSGMS HAVAKEETLK TSNGHSKPKT KKTGSKRLVM 70 80 90 100 110 120 LDPGHGGIDT GAIGRNGSQE KHVVLAIAKN VRAILRNHGI DARLTRTGDT FIPLYDRVEI 130 140 150 160 170 180 AHKHGADLFM SIHADGFTNP KAAGASVFAL SNRGASSAMA KYLSERENRA DEVAGKKATD 190 200 210 220 230 240 RDHLLQQVLF DLVQTDTIKN SLTLGSHILK KIKPIHKLHS RTTEQAAFVV LKSPSIPSVL 250 260 270 280 VETSFITNPE EERLLGTTAF RQKIATAIAN GIISYFHWFD NQKAHTKKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An oxygen-dependent coproporphyrinogen oxidase encoded by the hemF gene of Salmonella typhimurium." Xu K., Elliott T. J. Bacteriol. 175:4990-4999(1993) [PubMed: 8349542] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19503 Genomic DNA. Translation: AAA27138.1. AE006468 Genomic DNA. Translation: AAL21344.1.
PIR	A53302.
RefSeq	NP_461385.1.
3D structure databases
SMR	P33772. Positions 57-278.
ModBase	Search...
Proteomic databases
PRIDE	P33772.
Genome annotation databases
GeneID	1253972.
GenomeReviews	Gene locus STM2450 in contig AE006468_GR.
KEGG	stm:STM2450.
NMPDR	fig|99287.1.peg.2366.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG656726.
OMA	DRENRAD.
Enzyme and pathway databases
BioCyc	STYP99287:STM2450-MONOMER.
BRENDA	3.5.1.28. 2.
Family and domain databases
InterPro	IPR002508. CW_Hdrlase/autolysin_cat. IPR006311. TAT_signal. [Graphical view]
Gene3D	G3DSA:3.40.630.40. Cell_wall_OHase/autolysin_cat. 1 hit.
Pfam	PF01520. Amidase_3. 1 hit. [Graphical view]
SMART	SM00646. Ami_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMIA_SALTY

Accession

Primary (citable) accession number: P33772

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)