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P33767 (OSTB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1

Short name=Oligosaccharyl transferase subunit WBP1
EC=2.4.99.18
Alternative name(s):
Oligosaccharyl transferase subunit beta
Gene names
Name:WBP1
Ordered Locus Names:YEL002C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length430 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Catalytic activity

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. WBP1, SWP1 and OST2 probably form a subcomplex. May interact directly with OST2, OST3, OST5, OST6, WBP1 AND SWP1. Interacts with SEC61, SBH1 and SSS1. Ref.5 Ref.6 Ref.12 Ref.13

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Domain

The cytoplasmic C-terminal domain contains a functional dilysine-retrieval motif, which is involved in the retrograde Golgi-to-ER transport of the protein.

Miscellaneous

Present with 14900 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the DDOST 48 kDa subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 430410Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1
PRO_0000021961

Regions

Topological domain21 – 393373Lumenal Potential
Transmembrane394 – 41421Helical; Potential
Topological domain415 – 43016Cytoplasmic Potential

Amino acid modifications

Glycosylation601N-linked (GlcNAc...) Potential
Glycosylation3321N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P33767 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 495DA76BE8A9B29A

FASTA43049,392
        10         20         30         40         50         60 
MRTDWNFFFC ILLQAIFVVG TQTSRTLVLY DQSTEPLEEY SVYLKDLEQR NYKLEYLDIN 

        70         80         90        100        110        120 
STSTTVDLYD KEQRLFDNII VFPTKGGKNL ARQIPVKQLI KFFENEGNIL CMSSPGAVPN 

       130        140        150        160        170        180 
TIRLFLNELG IYPSPKGHVI RDYFSPSSEE LVVSSNHLLN KYVYNARKSE DFVFGESSAA 

       190        200        210        220        230        240 
LLENREQIVP ILNAPRTSFT ESKGKCNSWT SGSQGFLVVG FQNLNNARLV WIGSSDFLKN 

       250        260        270        280        290        300 
KNQDSNQEFA KELLKWTFNE KSVIKSVHAV HSHADGTSYD EEPYKIKDKV IYSVGFSEWN 

       310        320        330        340        350        360 
GEEWLPHIAD DIQFELRQVD PYYRLTLSPS GNDSETQYYT TGEFILPDRH GVFTFLTDYR 

       370        380        390        400        410        420 
KIGLSFTTDK DVKAIRHLAN DEYPRSWEIS NSWVYISAIC GVIVAWIFFV VSFVTTSSVG 

       430 
KKLETFKKTN 

« Hide

References

« Hide 'large scale' references
[1]"An essential 45 kDa yeast transmembrane protein reacts with anti-nuclear pore antibodies: purification of the protein, immunolocalization and cloning of the gene."
Te Heesen S., Rauhut R., Aebersold R., Abelson J., Aebi M., Clark M.W.
Eur. J. Cell Biol. 56:8-18(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The yeast WBP1 is essential for oligosaccharyl transferase activity in vivo and in vitro."
Te Heesen S., Janetzky B., Lehle L., Aebi M.
EMBO J. 11:2071-2075(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides."
Kelleher D.J., Gilmore R.
J. Biol. Chem. 269:12908-12917(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[6]"The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p."
Karaoglu D., Kelleher D.J., Gilmore R.
J. Biol. Chem. 272:32513-32520(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
[7]"The oligosaccharyltransferase complex from yeast."
Knauer R., Lehle L.
Biochim. Biophys. Acta 1426:259-273(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit."
Schwarz M., Knauer R., Lehle L.
FEBS Lett. 579:6564-6568(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[10]"The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast."
Spirig U., Bodmer D., Wacker M., Burda P., Aebi M.
Glycobiology 15:1396-1406(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[11]"Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons."
Yan A., Lennarz W.J.
Glycobiology 15:1407-1415(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
[12]"Subunits of the translocon interact with components of the oligosaccharyl transferase complex."
Chavan M., Yan A., Lennarz W.J.
J. Biol. Chem. 280:22917-22924(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SEC61; SBH1 AND SSS1.
[13]"Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions."
Yan A., Wu E., Lennarz W.J.
Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OST2; OST3; OST5; OST6; WBP1 AND SWP1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61388 Genomic DNA. Translation: CAA43660.1.
U18530 Genomic DNA. Translation: AAB64479.1.
BK006939 Genomic DNA. Translation: DAA07649.1.
PIRS20187.
RefSeqNP_010914.3. NM_001178817.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4J86X-ray1.48C/D425-430[»]
ProteinModelPortalP33767.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36729. 284 interactions.
DIPDIP-676N.
IntActP33767. 44 interactions.
MINTMINT-616984.
STRING4932.YEL002C.

Proteomic databases

PaxDbP33767.
PeptideAtlasP33767.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL002C; YEL002C; YEL002C.
GeneID856716.
KEGGsce:YEL002C.

Organism-specific databases

CYGDYEL002c.
SGDS000000728. WBP1.

Phylogenomic databases

eggNOGNOG263094.
GeneTreeENSGT00390000017294.
HOGENOMHOG000246553.
KOK12670.
OMAVPILNAP.
OrthoDBEOG7BS4KJ.

Enzyme and pathway databases

BioCycYEAST:YEL002C-MONOMER.
BRENDA2.4.1.119. 984.
UniPathwayUPA00378.

Gene expression databases

GenevestigatorP33767.

Family and domain databases

InterProIPR005013. WBP1.
[Graphical view]
PANTHERPTHR10830. PTHR10830. 1 hit.
PfamPF03345. DDOST_48kD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982799.
PROP33767.

Entry information

Entry nameOSTB_YEAST
AccessionPrimary (citable) accession number: P33767
Secondary accession number(s): D3DLP5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways