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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1

Gene

WBP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Miscellaneous

Present with 14900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • transferase activity, transferring glycosyl groups Source: SGD

GO - Biological processi

  • protein N-linked glycosylation Source: SGD
  • protein N-linked glycosylation via asparagine Source: InterPro

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YEL002C-MONOMER.
YEAST:YEL002C-MONOMER.
BRENDAi2.4.99.18. 984.
ReactomeiR-SCE-6798695. Neutrophil degranulation.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase subunit WBP1
Alternative name(s):
Oligosaccharyl transferase subunit beta
Gene namesi
Name:WBP1
Ordered Locus Names:YEL002C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL002C.
SGDiS000000728. WBP1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 393LumenalSequence analysisAdd BLAST373
Transmembranei394 – 414HelicalSequence analysisAdd BLAST21
Topological domaini415 – 430CytoplasmicSequence analysisAdd BLAST16

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000002196121 – 430Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1Add BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi332N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP33767.
PaxDbiP33767.
PRIDEiP33767.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. WBP1, SWP1 and OST2 probably form a subcomplex. May interact directly with OST2, OST3, OST5, OST6, WBP1 AND SWP1. Interacts with SEC61, SBH1 and SSS1.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36729. 462 interactors.
DIPiDIP-676N.
ELMiP33767.
IntActiP33767. 47 interactors.
MINTiP33767.
STRINGi4932.YEL002C.

Structurei

Secondary structure

1430
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 31Combined sources4
Turni32 – 34Combined sources3
Helixi38 – 40Combined sources3
Helixi41 – 49Combined sources9
Beta strandi56 – 59Combined sources4
Beta strandi68 – 74Combined sources7
Beta strandi77 – 81Combined sources5
Helixi89 – 93Combined sources5
Helixi96 – 103Combined sources8
Beta strandi108 – 112Combined sources5
Helixi120 – 128Combined sources9
Beta strandi131 – 133Combined sources3
Beta strandi139 – 142Combined sources4
Beta strandi146 – 154Combined sources9
Helixi155 – 157Combined sources3
Turni161 – 163Combined sources3
Beta strandi171 – 177Combined sources7
Beta strandi180 – 182Combined sources3
Beta strandi186 – 190Combined sources5
Beta strandi196 – 201Combined sources6
Beta strandi203 – 205Combined sources3
Turni212 – 214Combined sources3
Beta strandi216 – 222Combined sources7
Beta strandi228 – 232Combined sources5
Beta strandi237 – 239Combined sources3
Beta strandi244 – 247Combined sources4
Helixi248 – 257Combined sources10
Turni258 – 260Combined sources3
Beta strandi264 – 266Combined sources3
Beta strandi270 – 273Combined sources4
Turni279 – 281Combined sources3
Beta strandi285 – 287Combined sources3
Beta strandi289 – 299Combined sources11
Beta strandi304 – 306Combined sources3
Beta strandi313 – 326Combined sources14
Beta strandi337 – 340Combined sources4
Beta strandi351 – 359Combined sources9
Beta strandi367 – 376Combined sources10
Beta strandi380 – 383Combined sources4
Turni386 – 388Combined sources3
Beta strandi389 – 391Combined sources3
Helixi393 – 415Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4J86X-ray1.48C/D425-430[»]
6C26electron microscopy3.50B1-430[»]
6EZNelectron microscopy3.30G1-430[»]
ProteinModelPortaliP33767.
SMRiP33767.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The cytoplasmic C-terminal domain contains a functional dilysine-retrieval motif, which is involved in the retrograde Golgi-to-ER transport of the protein.

Sequence similaritiesi

Belongs to the DDOST 48 kDa subunit family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000017294.
HOGENOMiHOG000246553.
InParanoidiP33767.
KOiK12670.
OMAiAFSMMIG.
OrthoDBiEOG092C28Y9.

Family and domain databases

InterProiView protein in InterPro
IPR005013. DDOST_48_kDa_subunit.
PANTHERiPTHR10830. PTHR10830. 1 hit.
PfamiView protein in Pfam
PF03345. DDOST_48kD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33767-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTDWNFFFC ILLQAIFVVG TQTSRTLVLY DQSTEPLEEY SVYLKDLEQR
60 70 80 90 100
NYKLEYLDIN STSTTVDLYD KEQRLFDNII VFPTKGGKNL ARQIPVKQLI
110 120 130 140 150
KFFENEGNIL CMSSPGAVPN TIRLFLNELG IYPSPKGHVI RDYFSPSSEE
160 170 180 190 200
LVVSSNHLLN KYVYNARKSE DFVFGESSAA LLENREQIVP ILNAPRTSFT
210 220 230 240 250
ESKGKCNSWT SGSQGFLVVG FQNLNNARLV WIGSSDFLKN KNQDSNQEFA
260 270 280 290 300
KELLKWTFNE KSVIKSVHAV HSHADGTSYD EEPYKIKDKV IYSVGFSEWN
310 320 330 340 350
GEEWLPHIAD DIQFELRQVD PYYRLTLSPS GNDSETQYYT TGEFILPDRH
360 370 380 390 400
GVFTFLTDYR KIGLSFTTDK DVKAIRHLAN DEYPRSWEIS NSWVYISAIC
410 420 430
GVIVAWIFFV VSFVTTSSVG KKLETFKKTN
Length:430
Mass (Da):49,392
Last modified:February 1, 1994 - v1
Checksum:i495DA76BE8A9B29A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61388 Genomic DNA. Translation: CAA43660.1.
U18530 Genomic DNA. Translation: AAB64479.1.
BK006939 Genomic DNA. Translation: DAA07649.1.
PIRiS20187.
RefSeqiNP_010914.3. NM_001178817.3.

Genome annotation databases

EnsemblFungiiYEL002C; YEL002C; YEL002C.
GeneIDi856716.
KEGGisce:YEL002C.

Similar proteinsi

Entry informationi

Entry nameiOSTB_YEAST
AccessioniPrimary (citable) accession number: P33767
Secondary accession number(s): D3DLP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome