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P33767

- OSTB_YEAST

UniProt

P33767 - OSTB_YEAST

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Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1
Gene
WBP1, YEL002C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi

GO - Molecular functioni

  1. dolichyl-diphosphooligosaccharide-protein glycotransferase activity Source: InterPro
  2. protein binding Source: IntAct

GO - Biological processi

  1. mitotic cell cycle Source: SGD
  2. protein N-linked glycosylation Source: SGD
  3. protein N-linked glycosylation via asparagine Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YEL002C-MONOMER.
BRENDAi2.4.1.119. 984.
ReactomeiREACT_189235. Advanced glycosylation endproduct receptor signaling.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase subunit WBP1
Alternative name(s):
Oligosaccharyl transferase subunit beta
Gene namesi
Name:WBP1
Ordered Locus Names:YEL002C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL002c.
SGDiS000000728. WBP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 393373Lumenal Reviewed prediction
Add
BLAST
Transmembranei394 – 41421Helical; Reviewed prediction
Add
BLAST
Topological domaini415 – 43016Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. endoplasmic reticulum membrane Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
  4. nuclear envelope Source: SGD
  5. oligosaccharyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed prediction
Add
BLAST
Chaini21 – 430410Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1
PRO_0000021961Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi60 – 601N-linked (GlcNAc...) Reviewed prediction
Glycosylationi332 – 3321N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP33767.
PaxDbiP33767.
PeptideAtlasiP33767.

Expressioni

Gene expression databases

GenevestigatoriP33767.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. WBP1, SWP1 and OST2 probably form a subcomplex. May interact directly with OST2, OST3, OST5, OST6, WBP1 AND SWP1. Interacts with SEC61, SBH1 and SSS1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
COP1P536223EBI-12658,EBI-4860
SBH1P528702EBI-12658,EBI-16410
SEC61P329152EBI-12658,EBI-16400
SSS1P351792EBI-12658,EBI-16406
STT3P390073EBI-12658,EBI-18447
SWP1Q027954EBI-12658,EBI-12666

Protein-protein interaction databases

BioGridi36729. 284 interactions.
DIPiDIP-676N.
IntActiP33767. 44 interactions.
MINTiMINT-616984.
STRINGi4932.YEL002C.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4J86X-ray1.48C/D425-430[»]
ProteinModelPortaliP33767.

Family & Domainsi

Domaini

The cytoplasmic C-terminal domain contains a functional dilysine-retrieval motif, which is involved in the retrograde Golgi-to-ER transport of the protein.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG263094.
GeneTreeiENSGT00390000017294.
HOGENOMiHOG000246553.
KOiK12670.
OMAiHVIFFAP.
OrthoDBiEOG7BS4KJ.

Family and domain databases

InterProiIPR005013. WBP1.
[Graphical view]
PANTHERiPTHR10830. PTHR10830. 1 hit.
PfamiPF03345. DDOST_48kD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33767-1 [UniParc]FASTAAdd to Basket

« Hide

MRTDWNFFFC ILLQAIFVVG TQTSRTLVLY DQSTEPLEEY SVYLKDLEQR    50
NYKLEYLDIN STSTTVDLYD KEQRLFDNII VFPTKGGKNL ARQIPVKQLI 100
KFFENEGNIL CMSSPGAVPN TIRLFLNELG IYPSPKGHVI RDYFSPSSEE 150
LVVSSNHLLN KYVYNARKSE DFVFGESSAA LLENREQIVP ILNAPRTSFT 200
ESKGKCNSWT SGSQGFLVVG FQNLNNARLV WIGSSDFLKN KNQDSNQEFA 250
KELLKWTFNE KSVIKSVHAV HSHADGTSYD EEPYKIKDKV IYSVGFSEWN 300
GEEWLPHIAD DIQFELRQVD PYYRLTLSPS GNDSETQYYT TGEFILPDRH 350
GVFTFLTDYR KIGLSFTTDK DVKAIRHLAN DEYPRSWEIS NSWVYISAIC 400
GVIVAWIFFV VSFVTTSSVG KKLETFKKTN 430
Length:430
Mass (Da):49,392
Last modified:February 1, 1994 - v1
Checksum:i495DA76BE8A9B29A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61388 Genomic DNA. Translation: CAA43660.1.
U18530 Genomic DNA. Translation: AAB64479.1.
BK006939 Genomic DNA. Translation: DAA07649.1.
PIRiS20187.
RefSeqiNP_010914.3. NM_001178817.3.

Genome annotation databases

EnsemblFungiiYEL002C; YEL002C; YEL002C.
GeneIDi856716.
KEGGisce:YEL002C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61388 Genomic DNA. Translation: CAA43660.1 .
U18530 Genomic DNA. Translation: AAB64479.1 .
BK006939 Genomic DNA. Translation: DAA07649.1 .
PIRi S20187.
RefSeqi NP_010914.3. NM_001178817.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4J86 X-ray 1.48 C/D 425-430 [» ]
ProteinModelPortali P33767.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36729. 284 interactions.
DIPi DIP-676N.
IntActi P33767. 44 interactions.
MINTi MINT-616984.
STRINGi 4932.YEL002C.

Proteomic databases

MaxQBi P33767.
PaxDbi P33767.
PeptideAtlasi P33767.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL002C ; YEL002C ; YEL002C .
GeneIDi 856716.
KEGGi sce:YEL002C.

Organism-specific databases

CYGDi YEL002c.
SGDi S000000728. WBP1.

Phylogenomic databases

eggNOGi NOG263094.
GeneTreei ENSGT00390000017294.
HOGENOMi HOG000246553.
KOi K12670.
OMAi HVIFFAP.
OrthoDBi EOG7BS4KJ.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci YEAST:YEL002C-MONOMER.
BRENDAi 2.4.1.119. 984.
Reactomei REACT_189235. Advanced glycosylation endproduct receptor signaling.

Miscellaneous databases

NextBioi 982799.
PROi P33767.

Gene expression databases

Genevestigatori P33767.

Family and domain databases

InterProi IPR005013. WBP1.
[Graphical view ]
PANTHERi PTHR10830. PTHR10830. 1 hit.
Pfami PF03345. DDOST_48kD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An essential 45 kDa yeast transmembrane protein reacts with anti-nuclear pore antibodies: purification of the protein, immunolocalization and cloning of the gene."
    Te Heesen S., Rauhut R., Aebersold R., Abelson J., Aebi M., Clark M.W.
    Eur. J. Cell Biol. 56:8-18(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The yeast WBP1 is essential for oligosaccharyl transferase activity in vivo and in vitro."
    Te Heesen S., Janetzky B., Lehle L., Aebi M.
    EMBO J. 11:2071-2075(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides."
    Kelleher D.J., Gilmore R.
    J. Biol. Chem. 269:12908-12917(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
  6. "The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p."
    Karaoglu D., Kelleher D.J., Gilmore R.
    J. Biol. Chem. 272:32513-32520(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
  7. "The oligosaccharyltransferase complex from yeast."
    Knauer R., Lehle L.
    Biochim. Biophys. Acta 1426:259-273(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit."
    Schwarz M., Knauer R., Lehle L.
    FEBS Lett. 579:6564-6568(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
  10. "The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast."
    Spirig U., Bodmer D., Wacker M., Burda P., Aebi M.
    Glycobiology 15:1396-1406(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
  11. "Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons."
    Yan A., Lennarz W.J.
    Glycobiology 15:1407-1415(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
  12. "Subunits of the translocon interact with components of the oligosaccharyl transferase complex."
    Chavan M., Yan A., Lennarz W.J.
    J. Biol. Chem. 280:22917-22924(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SEC61; SBH1 AND SSS1.
  13. "Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions."
    Yan A., Wu E., Lennarz W.J.
    Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OST2; OST3; OST5; OST6; WBP1 AND SWP1.

Entry informationi

Entry nameiOSTB_YEAST
AccessioniPrimary (citable) accession number: P33767
Secondary accession number(s): D3DLP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 14900 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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