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P33767

- OSTB_YEAST

UniProt

P33767 - OSTB_YEAST

Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1

Gene

WBP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

    Catalytic activityi

    Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

    Pathwayi

    GO - Molecular functioni

    1. dolichyl-diphosphooligosaccharide-protein glycotransferase activity Source: InterPro
    2. protein binding Source: IntAct

    GO - Biological processi

    1. mitotic cell cycle Source: SGD
    2. protein N-linked glycosylation Source: SGD
    3. protein N-linked glycosylation via asparagine Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:YEL002C-MONOMER.
    BRENDAi2.4.1.119. 984.
    ReactomeiREACT_189235. Advanced glycosylation endproduct receptor signaling.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 (EC:2.4.99.18)
    Short name:
    Oligosaccharyl transferase subunit WBP1
    Alternative name(s):
    Oligosaccharyl transferase subunit beta
    Gene namesi
    Name:WBP1
    Ordered Locus Names:YEL002C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYEL002c.
    SGDiS000000728. WBP1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. endoplasmic reticulum membrane Source: SGD
    3. integral component of membrane Source: UniProtKB-KW
    4. nuclear envelope Source: SGD
    5. oligosaccharyltransferase complex Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 430410Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1PRO_0000021961Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi332 – 3321N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP33767.
    PaxDbiP33767.
    PeptideAtlasiP33767.

    Expressioni

    Gene expression databases

    GenevestigatoriP33767.

    Interactioni

    Subunit structurei

    Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. WBP1, SWP1 and OST2 probably form a subcomplex. May interact directly with OST2, OST3, OST5, OST6, WBP1 AND SWP1. Interacts with SEC61, SBH1 and SSS1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    COP1P536223EBI-12658,EBI-4860
    SBH1P528702EBI-12658,EBI-16410
    SEC61P329152EBI-12658,EBI-16400
    SSS1P351792EBI-12658,EBI-16406
    STT3P390073EBI-12658,EBI-18447
    SWP1Q027954EBI-12658,EBI-12666

    Protein-protein interaction databases

    BioGridi36729. 284 interactions.
    DIPiDIP-676N.
    IntActiP33767. 44 interactions.
    MINTiMINT-616984.
    STRINGi4932.YEL002C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4J86X-ray1.48C/D425-430[»]
    ProteinModelPortaliP33767.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini21 – 393373LumenalSequence AnalysisAdd
    BLAST
    Topological domaini415 – 43016CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei394 – 41421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    The cytoplasmic C-terminal domain contains a functional dilysine-retrieval motif, which is involved in the retrograde Golgi-to-ER transport of the protein.

    Sequence similaritiesi

    Belongs to the DDOST 48 kDa subunit family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG263094.
    GeneTreeiENSGT00390000017294.
    HOGENOMiHOG000246553.
    KOiK12670.
    OMAiHVIFFAP.
    OrthoDBiEOG7BS4KJ.

    Family and domain databases

    InterProiIPR005013. WBP1.
    [Graphical view]
    PANTHERiPTHR10830. PTHR10830. 1 hit.
    PfamiPF03345. DDOST_48kD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33767-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRTDWNFFFC ILLQAIFVVG TQTSRTLVLY DQSTEPLEEY SVYLKDLEQR    50
    NYKLEYLDIN STSTTVDLYD KEQRLFDNII VFPTKGGKNL ARQIPVKQLI 100
    KFFENEGNIL CMSSPGAVPN TIRLFLNELG IYPSPKGHVI RDYFSPSSEE 150
    LVVSSNHLLN KYVYNARKSE DFVFGESSAA LLENREQIVP ILNAPRTSFT 200
    ESKGKCNSWT SGSQGFLVVG FQNLNNARLV WIGSSDFLKN KNQDSNQEFA 250
    KELLKWTFNE KSVIKSVHAV HSHADGTSYD EEPYKIKDKV IYSVGFSEWN 300
    GEEWLPHIAD DIQFELRQVD PYYRLTLSPS GNDSETQYYT TGEFILPDRH 350
    GVFTFLTDYR KIGLSFTTDK DVKAIRHLAN DEYPRSWEIS NSWVYISAIC 400
    GVIVAWIFFV VSFVTTSSVG KKLETFKKTN 430
    Length:430
    Mass (Da):49,392
    Last modified:February 1, 1994 - v1
    Checksum:i495DA76BE8A9B29A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61388 Genomic DNA. Translation: CAA43660.1.
    U18530 Genomic DNA. Translation: AAB64479.1.
    BK006939 Genomic DNA. Translation: DAA07649.1.
    PIRiS20187.
    RefSeqiNP_010914.3. NM_001178817.3.

    Genome annotation databases

    EnsemblFungiiYEL002C; YEL002C; YEL002C.
    GeneIDi856716.
    KEGGisce:YEL002C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61388 Genomic DNA. Translation: CAA43660.1 .
    U18530 Genomic DNA. Translation: AAB64479.1 .
    BK006939 Genomic DNA. Translation: DAA07649.1 .
    PIRi S20187.
    RefSeqi NP_010914.3. NM_001178817.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4J86 X-ray 1.48 C/D 425-430 [» ]
    ProteinModelPortali P33767.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36729. 284 interactions.
    DIPi DIP-676N.
    IntActi P33767. 44 interactions.
    MINTi MINT-616984.
    STRINGi 4932.YEL002C.

    Proteomic databases

    MaxQBi P33767.
    PaxDbi P33767.
    PeptideAtlasi P33767.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YEL002C ; YEL002C ; YEL002C .
    GeneIDi 856716.
    KEGGi sce:YEL002C.

    Organism-specific databases

    CYGDi YEL002c.
    SGDi S000000728. WBP1.

    Phylogenomic databases

    eggNOGi NOG263094.
    GeneTreei ENSGT00390000017294.
    HOGENOMi HOG000246553.
    KOi K12670.
    OMAi HVIFFAP.
    OrthoDBi EOG7BS4KJ.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci YEAST:YEL002C-MONOMER.
    BRENDAi 2.4.1.119. 984.
    Reactomei REACT_189235. Advanced glycosylation endproduct receptor signaling.

    Miscellaneous databases

    NextBioi 982799.
    PROi P33767.

    Gene expression databases

    Genevestigatori P33767.

    Family and domain databases

    InterProi IPR005013. WBP1.
    [Graphical view ]
    PANTHERi PTHR10830. PTHR10830. 1 hit.
    Pfami PF03345. DDOST_48kD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An essential 45 kDa yeast transmembrane protein reacts with anti-nuclear pore antibodies: purification of the protein, immunolocalization and cloning of the gene."
      Te Heesen S., Rauhut R., Aebersold R., Abelson J., Aebi M., Clark M.W.
      Eur. J. Cell Biol. 56:8-18(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The yeast WBP1 is essential for oligosaccharyl transferase activity in vivo and in vitro."
      Te Heesen S., Janetzky B., Lehle L., Aebi M.
      EMBO J. 11:2071-2075(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides."
      Kelleher D.J., Gilmore R.
      J. Biol. Chem. 269:12908-12917(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
    6. "The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p."
      Karaoglu D., Kelleher D.J., Gilmore R.
      J. Biol. Chem. 272:32513-32520(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE COMPLEX.
    7. "The oligosaccharyltransferase complex from yeast."
      Knauer R., Lehle L.
      Biochim. Biophys. Acta 1426:259-273(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON OLIGOSACCHARYL TRANSFERASE.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit."
      Schwarz M., Knauer R., Lehle L.
      FEBS Lett. 579:6564-6568(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
    10. "The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast."
      Spirig U., Bodmer D., Wacker M., Burda P., Aebi M.
      Glycobiology 15:1396-1406(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
    11. "Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons."
      Yan A., Lennarz W.J.
      Glycobiology 15:1407-1415(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF OLIGOSACCHARYL TRANSFERASE COMPLEXES.
    12. "Subunits of the translocon interact with components of the oligosaccharyl transferase complex."
      Chavan M., Yan A., Lennarz W.J.
      J. Biol. Chem. 280:22917-22924(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SEC61; SBH1 AND SSS1.
    13. "Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions."
      Yan A., Wu E., Lennarz W.J.
      Proc. Natl. Acad. Sci. U.S.A. 102:7121-7126(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH OST2; OST3; OST5; OST6; WBP1 AND SWP1.

    Entry informationi

    Entry nameiOSTB_YEAST
    AccessioniPrimary (citable) accession number: P33767
    Secondary accession number(s): D3DLP5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 14900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3