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Reviewed, UniProtKB/Swiss-Prot P33765 (AA3R_HUMAN)

Last modified January 19, 2010. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Adenosine A3 receptor
Gene names
Name: ADORA3
ORF Names: UNQ1931/PRO4406
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Phosphorylation on Ser-317 may be crucial for rapid desensitization By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P33765-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P33765-2)

The sequence of this isoform differs from the canonical sequence as follows:
     118-318: YKRVTTHRRI...LDTSIEKNSE → FRIPGLPGCI...PKEMAPTEQM

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Adenosine A3 receptor
PRO_0000069010

Regions

Topological domain1 – 1414Extracellular By similarity
Transmembrane15 – 37231 By similarity
Topological domain38 – 4811Cytoplasmic By similarity
Transmembrane49 – 72242 By similarity
Topological domain73 – 8412Extracellular By similarity
Transmembrane85 – 106223 By similarity
Topological domain107 – 12620Cytoplasmic By similarity
Transmembrane127 – 148224 By similarity
Topological domain149 – 17729Extracellular By similarity
Transmembrane178 – 198215 By similarity
Topological domain199 – 23133Cytoplasmic By similarity
Transmembrane232 – 255246 By similarity
Topological domain256 – 2616Extracellular By similarity
Transmembrane262 – 284237 By similarity
Topological domain285 – 31834Cytoplasmic By similarity

Amino acid modifications

Modified residue3171Phosphoserine By similarity
Lipidation3031S-palmitoyl cysteine Potential
Glycosylation31N-linked (GlcNAc...) Potential
Glycosylation41N-linked (GlcNAc...) Potential
Glycosylation121N-linked (GlcNAc...) Potential
Disulfide bond83 ↔ 166 By similarity

Natural variations

Alternative sequence118 – 318201YKRVT…EKNSE → FRIPGLPGCILSFQLKVCFL PVMWLFILLSLALISDAMVM DEKVKRSFVLDTASAICNYN AHYKNHPKYWCRGYFRDYCN IIAFSPNSTNHVALRDTGNQ LIVTMSCLTKEDTGWYWCGI QRDFARDDMDFTELIVTDDK GTLANDFWSGKDLSGNKTRS CKAPKVVRKADRSRTSILII CILITGLGIISVISHLTKRR RSQRNRRVGNTLKPFSRVLT PKEMAPTEQM in isoform 2.
VSP_013320
Natural variant1051A → T in a colorectal cancer sample; somatic mutation. Ref.10
VAR_035755
Natural variant2481I → L: dbSNP rs35511654. Ref.3
VAR_049366
Natural variant2661M → K: dbSNP rs2800889.
VAR_049367

Experimental info

Sequence conflict71A → T in AAA16365. Ref.1
Sequence conflict1751D → V Ref.9

Secondary structure

1 ...................................................... 318
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 690E67986130FC28

FASTA31836,185
        10         20         30         40         50         60 
MPNNSTALSL ANVTYITMEI FIGLCAIVGN VLVICVVKLN PSLQTTTFYF IVSLALADIA 

        70         80         90        100        110        120 
VGVLVMPLAI VVSLGITIHF YSCLFMTCLL LIFTHASIMS LLAIAVDRYL RVKLTVRYKR 

       130        140        150        160        170        180 
VTTHRRIWLA LGLCWLVSFL VGLTPMFGWN MKLTSEYHRN VTFLSCQFVS VMRMDYMVYF 

       190        200        210        220        230        240 
SFLTWIFIPL VVMCAIYLDI FYIIRNKLSL NLSNSKETGA FYGREFKTAK SLFLVLFLFA 

       250        260        270        280        290        300 
LSWLPLSIIN CIIYFNGEVP QLVLYMGILL SHANSMMNPI VYAYKIKKFK ETYLLILKAC 

       310 
VVCHPSDSLD TSIEKNSE

« Hide

Isoform 2.

Checksum: 7F4974135F4F04A6
Show »

FASTA34738,976

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References

« Hide 'large scale' references
[1]"cDNA cloning and sequence analysis of the human A3 adenosine receptor."
Sajjadi F.G., Firestein G.S.
Biochim. Biophys. Acta 1179:105-107(1993) [PubMed: 8399349] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Heart.
[2]"Molecular cloning and characterization of the human A3 adenosine receptor."
Salvatore C.A., Jacobson M.A., Taylor H.E., Linden J., Johnson R.G.
Proc. Natl. Acad. Sci. U.S.A. 90:10365-10369(1993) [PubMed: 8234299] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[3]Meyerehof W., Hamm B., Schoenrock C., Fehr S., Wulfsen I., Richter D.
Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT LEU-248.
Tissue: Testis.
[4]Atkinson M.R., Townsend-Nicholson A., Nicholl J.K., Sutherland G.R., Schofield P.R.
Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Testis.
[5]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[6]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[7]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed: 16710414] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain, Lung and Testis.
[9]"Molecular phylogenetics and the origins of placental mammals."
Murphy W.J., Eizirik E., Johnson W.E., Zhang Y.-P., Ryder O.A., O'Brien S.J.
Nature 409:614-618(2001) [PubMed: 11214319] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 166-272 (ISOFORM 1).
[10]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed: 16959974] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-105.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L20463 mRNA. Translation: AAA16365.1.
L22607 mRNA. Translation: AAA35949.1.
X76981 mRNA. Translation: CAA54288.1.
L77730, L77729 Genomic DNA. Translation: AAB02790.1.
AY136749 mRNA. Translation: AAN01275.1.
AY358644 mRNA. Translation: AAQ89007.1.
AL390195 Genomic DNA. Translation: CAC36045.1.
AL390195 Genomic DNA. Translation: CAM21993.1.
BC029831 mRNA. Translation: AAH29831.1.
AY011231 Genomic DNA. Translation: AAG35155.1.
IPIIPI00016753.
IPI00419658.
PIRS38511.
RefSeqNP_000668.1.
NP_065734.5.
UniGeneHs.281342

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OEAmodel-A1-298[»]
1R7Nmodel-A1-318[»]
SMRP33765. Positions 12-318.
ModBaseSearch...

Protein-protein interaction databases

STRINGP33765.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP33765.

Proteomic databases

PRIDEP33765.

Genome annotation databases

EnsemblENST00000241356; ENSP00000241356; ENSG00000121933; Homo sapiens. [Genome view]
GeneID140.
KEGGhsa:140.
UCSCuc001ebh.2. human.

Organism-specific databases

CTD140.
GeneCardsGC01M111827.
H-InvDBHIX0000881.
HGNCHGNC:268. ADORA3.
MIM600445. gene.
PharmGKBPA24589.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG17768.
HOVERGENP33765.
OMAMVYFSFF.

Enzyme and pathway databases

ReactomeREACT_14797. Signaling by GPCR.

Gene expression databases

ArrayExpressP33765.
BgeeP33765.
CleanExHS_ADORA3.
GenevestigatorP33765.
GermOnlineENSG00000121933. Homo sapiens.

Family and domain databases

InterProIPR000276. 7TM_GPCR_Rhodpsn.
IPR000466. Adeno_A3_rcpt.
IPR001634. Adenosn_rcpt.
IPR017452. GPCR_Rhodpsn_supfam.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00555. ADENOSINEA3R.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00640. Adenosine.
DB01223. Aminophylline.
NextBio553.
SOURCESearch...

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Entry information

Entry nameAA3R_HUMAN
AccessionPrimary (citable) accession number: P33765
Secondary accession number(s): A2A3P4, Q6UWU0, Q9BYZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1996
Last modified: January 19, 2010
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents