Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P33765

- AA3R_HUMAN

UniProt

P33765 - AA3R_HUMAN

Protein

Adenosine receptor A3

Gene

ADORA3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction.

    GO - Molecular functioni

    1. G-protein coupled adenosine receptor activity Source: Ensembl

    GO - Biological processi

    1. activation of adenylate cyclase activity Source: ProtInc
    2. histamine secretion by mast cell Source: Ensembl
    3. inflammatory response Source: ProtInc
    4. positive regulation of calcium-mediated signaling Source: Ensembl
    5. positive regulation of inflammatory response Source: Ensembl
    6. positive regulation of leukocyte migration Source: Ensembl
    7. positive regulation of mast cell degranulation Source: Ensembl
    8. positive regulation of mucus secretion Source: Ensembl
    9. positive regulation of phosphatidylinositol 3-kinase signaling Source: Ensembl
    10. regulation of heart contraction Source: ProtInc
    11. response to wounding Source: ProtInc
    12. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_18288. Adenosine P1 receptors.
    REACT_19231. G alpha (i) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenosine receptor A3
    Gene namesi
    Name:ADORA3
    ORF Names:UNQ1931/PRO4406
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:268. ADORA3.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: ProtInc
    2. mast cell granule Source: GOC
    3. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24589.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 318318Adenosine receptor A3PRO_0000069010Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi3 – 31N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi4 – 41N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi12 – 121N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi83 ↔ 166PROSITE-ProRule annotation
    Lipidationi303 – 3031S-palmitoyl cysteineSequence Analysis

    Post-translational modificationi

    Phosphorylation on Ser-317 may be crucial for rapid desensitization.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiP33765.
    PRIDEiP33765.

    PTM databases

    PhosphoSiteiP33765.

    Expressioni

    Gene expression databases

    ArrayExpressiP33765.
    BgeeiP33765.
    CleanExiHS_ADORA3.
    GenevestigatoriP33765.

    Organism-specific databases

    HPAiHPA028509.
    HPA028566.
    HPA028568.

    Interactioni

    Protein-protein interaction databases

    BioGridi106650. 2 interactions.
    STRINGi9606.ENSP00000358730.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OEAmodel-A1-298[»]
    1R7Nmodel-A1-318[»]
    ProteinModelPortaliP33765.
    SMRiP33765. Positions 10-302.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1414ExtracellularBy similarityAdd
    BLAST
    Topological domaini38 – 4811CytoplasmicBy similarityAdd
    BLAST
    Topological domaini73 – 8412ExtracellularBy similarityAdd
    BLAST
    Topological domaini107 – 12620CytoplasmicBy similarityAdd
    BLAST
    Topological domaini149 – 17729ExtracellularBy similarityAdd
    BLAST
    Topological domaini199 – 23133CytoplasmicBy similarityAdd
    BLAST
    Topological domaini256 – 2616ExtracellularBy similarity
    Topological domaini285 – 31834CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 3723Helical; Name=1By similarityAdd
    BLAST
    Transmembranei49 – 7224Helical; Name=2By similarityAdd
    BLAST
    Transmembranei85 – 10622Helical; Name=3By similarityAdd
    BLAST
    Transmembranei127 – 14822Helical; Name=4By similarityAdd
    BLAST
    Transmembranei178 – 19821Helical; Name=5By similarityAdd
    BLAST
    Transmembranei232 – 25524Helical; Name=6By similarityAdd
    BLAST
    Transmembranei262 – 28423Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG133889.
    HOGENOMiHOG000015770.
    HOVERGENiHBG106962.
    KOiK04268.
    OMAiQEVARMQ.
    PhylomeDBiP33765.
    TreeFamiTF334441.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000466. Adeno_A3_rcpt.
    IPR001634. Adenosn_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00555. ADENOSINEA3R.
    PR00424. ADENOSINER.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P33765-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPNNSTALSL ANVTYITMEI FIGLCAIVGN VLVICVVKLN PSLQTTTFYF    50
    IVSLALADIA VGVLVMPLAI VVSLGITIHF YSCLFMTCLL LIFTHASIMS 100
    LLAIAVDRYL RVKLTVRYKR VTTHRRIWLA LGLCWLVSFL VGLTPMFGWN 150
    MKLTSEYHRN VTFLSCQFVS VMRMDYMVYF SFLTWIFIPL VVMCAIYLDI 200
    FYIIRNKLSL NLSNSKETGA FYGREFKTAK SLFLVLFLFA LSWLPLSIIN 250
    CIIYFNGEVP QLVLYMGILL SHANSMMNPI VYAYKIKKFK ETYLLILKAC 300
    VVCHPSDSLD TSIEKNSE 318
    Length:318
    Mass (Da):36,185
    Last modified:February 1, 1996 - v2
    Checksum:i690E67986130FC28
    GO
    Isoform 2 (identifier: P33765-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         118-318: YKRVTTHRRI...LDTSIEKNSE → FRIPGLPGCI...PKEMAPTEQM

    Show »
    Length:347
    Mass (Da):38,976
    Checksum:i7F4974135F4F04A6
    GO
    Isoform 3 (identifier: Q6P2N6-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry Q6P2N6.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:266
    Mass (Da):30,327
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71A → T in AAA16365. (PubMed:8399349)Curated
    Sequence conflicti175 – 1751D → V(PubMed:11214319)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti105 – 1051A → T in a colorectal cancer sample; somatic mutation. 1 Publication
    VAR_035755
    Natural varianti248 – 2481I → L.1 Publication
    Corresponds to variant rs35511654 [ dbSNP | Ensembl ].
    VAR_049366
    Natural varianti266 – 2661M → K.
    Corresponds to variant rs2800889 [ dbSNP | Ensembl ].
    VAR_049367

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei118 – 318201YKRVT…EKNSE → FRIPGLPGCILSFQLKVCFL PVMWLFILLSLALISDAMVM DEKVKRSFVLDTASAICNYN AHYKNHPKYWCRGYFRDYCN IIAFSPNSTNHVALRDTGNQ LIVTMSCLTKEDTGWYWCGI QRDFARDDMDFTELIVTDDK GTLANDFWSGKDLSGNKTRS CKAPKVVRKADRSRTSILII CILITGLGIISVISHLTKRR RSQRNRRVGNTLKPFSRVLT PKEMAPTEQM in isoform 2. 1 PublicationVSP_013320Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20463 mRNA. Translation: AAA16365.1.
    L22607 mRNA. Translation: AAA35949.1.
    X76981 mRNA. Translation: CAA54288.1.
    L77730, L77729 Genomic DNA. Translation: AAB02790.1.
    AY136749 mRNA. Translation: AAN01275.1.
    AY358644 mRNA. Translation: AAQ89007.1.
    AL390195 Genomic DNA. Translation: CAC36045.1.
    AL390195 Genomic DNA. Translation: CAM21993.1.
    BC029831 mRNA. Translation: AAH29831.1.
    AY011231 Genomic DNA. Translation: AAG35155.1.
    CCDSiCCDS838.1. [P33765-2]
    CCDS839.1. [P33765-1]
    PIRiS38511.
    RefSeqiNP_000668.1. NM_000677.3. [P33765-1]
    NP_065734.5. NM_020683.6. [P33765-2]
    UniGeneiHs.281342.

    Genome annotation databases

    EnsembliENST00000241356; ENSP00000241356; ENSG00000121933. [P33765-1]
    ENST00000369716; ENSP00000358730; ENSG00000121933. [P33765-2]
    GeneIDi140.
    KEGGihsa:140.
    UCSCiuc001ebf.3. human. [P33765-2]
    uc001ebh.4. human. [P33765-1]

    Polymorphism databases

    DMDMi1351831.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20463 mRNA. Translation: AAA16365.1 .
    L22607 mRNA. Translation: AAA35949.1 .
    X76981 mRNA. Translation: CAA54288.1 .
    L77730 , L77729 Genomic DNA. Translation: AAB02790.1 .
    AY136749 mRNA. Translation: AAN01275.1 .
    AY358644 mRNA. Translation: AAQ89007.1 .
    AL390195 Genomic DNA. Translation: CAC36045.1 .
    AL390195 Genomic DNA. Translation: CAM21993.1 .
    BC029831 mRNA. Translation: AAH29831.1 .
    AY011231 Genomic DNA. Translation: AAG35155.1 .
    CCDSi CCDS838.1. [P33765-2 ]
    CCDS839.1. [P33765-1 ]
    PIRi S38511.
    RefSeqi NP_000668.1. NM_000677.3. [P33765-1 ]
    NP_065734.5. NM_020683.6. [P33765-2 ]
    UniGenei Hs.281342.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OEA model - A 1-298 [» ]
    1R7N model - A 1-318 [» ]
    ProteinModelPortali P33765.
    SMRi P33765. Positions 10-302.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 106650. 2 interactions.
    STRINGi 9606.ENSP00000358730.

    Chemistry

    BindingDBi P33765.
    ChEMBLi CHEMBL2095195.
    DrugBanki DB00640. Adenosine.
    DB01223. Aminophylline.
    GuidetoPHARMACOLOGYi 21.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P33765.

    Polymorphism databases

    DMDMi 1351831.

    Proteomic databases

    PaxDbi P33765.
    PRIDEi P33765.

    Protocols and materials databases

    DNASUi 140.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000241356 ; ENSP00000241356 ; ENSG00000121933 . [P33765-1 ]
    ENST00000369716 ; ENSP00000358730 ; ENSG00000121933 . [P33765-2 ]
    GeneIDi 140.
    KEGGi hsa:140.
    UCSCi uc001ebf.3. human. [P33765-2 ]
    uc001ebh.4. human. [P33765-1 ]

    Organism-specific databases

    CTDi 140.
    GeneCardsi GC01M112025.
    HGNCi HGNC:268. ADORA3.
    HPAi HPA028509.
    HPA028566.
    HPA028568.
    MIMi 600445. gene.
    neXtProti NX_P33765.
    PharmGKBi PA24589.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG133889.
    HOGENOMi HOG000015770.
    HOVERGENi HBG106962.
    KOi K04268.
    OMAi QEVARMQ.
    PhylomeDBi P33765.
    TreeFami TF334441.

    Enzyme and pathway databases

    Reactomei REACT_18288. Adenosine P1 receptors.
    REACT_19231. G alpha (i) signalling events.

    Miscellaneous databases

    GeneWikii Adenosine_A3_receptor.
    GenomeRNAii 140.
    NextBioi 553.
    PROi P33765.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P33765.
    Bgeei P33765.
    CleanExi HS_ADORA3.
    Genevestigatori P33765.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000466. Adeno_A3_rcpt.
    IPR001634. Adenosn_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00555. ADENOSINEA3R.
    PR00424. ADENOSINER.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and sequence analysis of the human A3 adenosine receptor."
      Sajjadi F.G., Firestein G.S.
      Biochim. Biophys. Acta 1179:105-107(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Heart.
    2. "Molecular cloning and characterization of the human A3 adenosine receptor."
      Salvatore C.A., Jacobson M.A., Taylor H.E., Linden J., Johnson R.G.
      Proc. Natl. Acad. Sci. U.S.A. 90:10365-10369(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. Meyerehof W., Hamm B., Schoenrock C., Fehr S., Wulfsen I., Richter D.
      Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT LEU-248.
      Tissue: Testis.
    4. Atkinson M.R., Townsend-Nicholson A., Nicholl J.K., Sutherland G.R., Schofield P.R.
      Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Testis.
    5. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    7. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain, Lung and Testis.
    9. "Molecular phylogenetics and the origins of placental mammals."
      Murphy W.J., Eizirik E., Johnson W.E., Zhang Y.-P., Ryder O.A., O'Brien S.J.
      Nature 409:614-618(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 166-272 (ISOFORM 1).
    10. Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-105.

    Entry informationi

    Entry nameiAA3R_HUMAN
    AccessioniPrimary (citable) accession number: P33765
    Secondary accession number(s): A2A3P4, Q6UWU0, Q9BYZ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 145 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3