Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P33765 (AA3R_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 143. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenosine receptor A3
Gene names
Name:ADORA3
ORF Names:UNQ1931/PRO4406
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Phosphorylation on Ser-317 may be crucial for rapid desensitization By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of adenylate cyclase activity

Traceable author statement PubMed 9380026. Source: ProtInc

histamine secretion by mast cell

Inferred from electronic annotation. Source: Ensembl

inflammatory response

Traceable author statement PubMed 9164961. Source: ProtInc

positive regulation of calcium-mediated signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of inflammatory response

Inferred from electronic annotation. Source: Ensembl

positive regulation of leukocyte migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of mast cell degranulation

Inferred from electronic annotation. Source: Ensembl

positive regulation of mucus secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of phosphatidylinositol 3-kinase signaling

Inferred from electronic annotation. Source: Ensembl

regulation of heart contraction

Traceable author statement PubMed 9837869. Source: ProtInc

response to wounding

Traceable author statement PubMed 9837869. Source: ProtInc

signal transduction

Traceable author statement Ref.1PubMed 9380026. Source: ProtInc

   Cellular_componentintegral component of plasma membrane

Traceable author statement Ref.1. Source: ProtInc

mast cell granule

Inferred from electronic annotation. Source: GOC

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionG-protein coupled adenosine receptor activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P33765-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P33765-2)

The sequence of this isoform differs from the canonical sequence as follows:
     118-318: YKRVTTHRRI...LDTSIEKNSE → FRIPGLPGCI...PKEMAPTEQM
Isoform 3 (identifier: Q6P2N6-1)

The sequence of this isoform can be found in the external entry Q6P2N6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Adenosine receptor A3
PRO_0000069010

Regions

Topological domain1 – 1414Extracellular By similarity
Transmembrane15 – 3723Helical; Name=1; By similarity
Topological domain38 – 4811Cytoplasmic By similarity
Transmembrane49 – 7224Helical; Name=2; By similarity
Topological domain73 – 8412Extracellular By similarity
Transmembrane85 – 10622Helical; Name=3; By similarity
Topological domain107 – 12620Cytoplasmic By similarity
Transmembrane127 – 14822Helical; Name=4; By similarity
Topological domain149 – 17729Extracellular By similarity
Transmembrane178 – 19821Helical; Name=5; By similarity
Topological domain199 – 23133Cytoplasmic By similarity
Transmembrane232 – 25524Helical; Name=6; By similarity
Topological domain256 – 2616Extracellular By similarity
Transmembrane262 – 28423Helical; Name=7; By similarity
Topological domain285 – 31834Cytoplasmic By similarity

Amino acid modifications

Lipidation3031S-palmitoyl cysteine Potential
Glycosylation31N-linked (GlcNAc...) Potential
Glycosylation41N-linked (GlcNAc...) Potential
Glycosylation121N-linked (GlcNAc...) Potential
Disulfide bond83 ↔ 166 By similarity

Natural variations

Alternative sequence118 – 318201YKRVT…EKNSE → FRIPGLPGCILSFQLKVCFL PVMWLFILLSLALISDAMVM DEKVKRSFVLDTASAICNYN AHYKNHPKYWCRGYFRDYCN IIAFSPNSTNHVALRDTGNQ LIVTMSCLTKEDTGWYWCGI QRDFARDDMDFTELIVTDDK GTLANDFWSGKDLSGNKTRS CKAPKVVRKADRSRTSILII CILITGLGIISVISHLTKRR RSQRNRRVGNTLKPFSRVLT PKEMAPTEQM in isoform 2.
VSP_013320
Natural variant1051A → T in a colorectal cancer sample; somatic mutation. Ref.10
VAR_035755
Natural variant2481I → L. Ref.3
Corresponds to variant rs35511654 [ dbSNP | Ensembl ].
VAR_049366
Natural variant2661M → K.
Corresponds to variant rs2800889 [ dbSNP | Ensembl ].
VAR_049367

Experimental info

Sequence conflict71A → T in AAA16365. Ref.1
Sequence conflict1751D → V Ref.9

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 690E67986130FC28

FASTA31836,185
        10         20         30         40         50         60 
MPNNSTALSL ANVTYITMEI FIGLCAIVGN VLVICVVKLN PSLQTTTFYF IVSLALADIA 

        70         80         90        100        110        120 
VGVLVMPLAI VVSLGITIHF YSCLFMTCLL LIFTHASIMS LLAIAVDRYL RVKLTVRYKR 

       130        140        150        160        170        180 
VTTHRRIWLA LGLCWLVSFL VGLTPMFGWN MKLTSEYHRN VTFLSCQFVS VMRMDYMVYF 

       190        200        210        220        230        240 
SFLTWIFIPL VVMCAIYLDI FYIIRNKLSL NLSNSKETGA FYGREFKTAK SLFLVLFLFA 

       250        260        270        280        290        300 
LSWLPLSIIN CIIYFNGEVP QLVLYMGILL SHANSMMNPI VYAYKIKKFK ETYLLILKAC 

       310 
VVCHPSDSLD TSIEKNSE 

« Hide

Isoform 2 [UniParc].

Checksum: 7F4974135F4F04A6
Show »

FASTA34738,976
Isoform 3 [UniParc].

See Q6P2N6.

References

« Hide 'large scale' references
[1]"cDNA cloning and sequence analysis of the human A3 adenosine receptor."
Sajjadi F.G., Firestein G.S.
Biochim. Biophys. Acta 1179:105-107(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Heart.
[2]"Molecular cloning and characterization of the human A3 adenosine receptor."
Salvatore C.A., Jacobson M.A., Taylor H.E., Linden J., Johnson R.G.
Proc. Natl. Acad. Sci. U.S.A. 90:10365-10369(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[3]Meyerehof W., Hamm B., Schoenrock C., Fehr S., Wulfsen I., Richter D.
Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT LEU-248.
Tissue: Testis.
[4]Atkinson M.R., Townsend-Nicholson A., Nicholl J.K., Sutherland G.R., Schofield P.R.
Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Testis.
[5]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[6]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[7]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain, Lung and Testis.
[9]"Molecular phylogenetics and the origins of placental mammals."
Murphy W.J., Eizirik E., Johnson W.E., Zhang Y.-P., Ryder O.A., O'Brien S.J.
Nature 409:614-618(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 166-272 (ISOFORM 1).
[10]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-105.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L20463 mRNA. Translation: AAA16365.1.
L22607 mRNA. Translation: AAA35949.1.
X76981 mRNA. Translation: CAA54288.1.
L77730, L77729 Genomic DNA. Translation: AAB02790.1.
AY136749 mRNA. Translation: AAN01275.1.
AY358644 mRNA. Translation: AAQ89007.1.
AL390195 Genomic DNA. Translation: CAC36045.1.
AL390195 Genomic DNA. Translation: CAM21993.1.
BC029831 mRNA. Translation: AAH29831.1.
AY011231 Genomic DNA. Translation: AAG35155.1.
CCDSCCDS838.1. [P33765-2]
CCDS839.1. [P33765-1]
PIRS38511.
RefSeqNP_000668.1. NM_000677.3. [P33765-1]
NP_065734.5. NM_020683.6. [P33765-2]
UniGeneHs.281342.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OEAmodel-A1-298[»]
1R7Nmodel-A1-318[»]
ProteinModelPortalP33765.
SMRP33765. Positions 10-302.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid106650. 2 interactions.
STRING9606.ENSP00000358730.

Chemistry

BindingDBP33765.
ChEMBLCHEMBL2095195.
DrugBankDB00640. Adenosine.
DB01223. Aminophylline.
GuidetoPHARMACOLOGY21.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP33765.

Polymorphism databases

DMDM1351831.

Proteomic databases

PaxDbP33765.
PRIDEP33765.

Protocols and materials databases

DNASU140.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000241356; ENSP00000241356; ENSG00000121933. [P33765-1]
ENST00000369716; ENSP00000358730; ENSG00000121933. [P33765-2]
GeneID140.
KEGGhsa:140.
UCSCuc001ebf.3. human. [P33765-2]
uc001ebh.4. human. [P33765-1]

Organism-specific databases

CTD140.
GeneCardsGC01M112025.
HGNCHGNC:268. ADORA3.
HPAHPA028509.
HPA028566.
HPA028568.
MIM600445. gene.
neXtProtNX_P33765.
PharmGKBPA24589.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG133889.
HOGENOMHOG000015770.
HOVERGENHBG106962.
KOK04268.
OMAQEVARMQ.
PhylomeDBP33765.
TreeFamTF334441.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP33765.
BgeeP33765.
CleanExHS_ADORA3.
GenevestigatorP33765.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000466. Adeno_A3_rcpt.
IPR001634. Adenosn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00555. ADENOSINEA3R.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiAdenosine_A3_receptor.
GenomeRNAi140.
NextBio553.
PROP33765.
SOURCESearch...

Entry information

Entry nameAA3R_HUMAN
AccessionPrimary (citable) accession number: P33765
Secondary accession number(s): A2A3P4, Q6UWU0, Q9BYZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries