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P33764 (S10A3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein S100-A3
Alternative name(s):
Protein S-100E
S100 calcium-binding protein A3
Gene names
Name:S100A3
Synonyms:S100E
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length101 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds both calcium and zinc. May be involved in calcium-dependent cuticle cell differentiation, hair shaft and hair cuticular barrier formation. Ref.9

Subunit structure

Homodimer and homotetramer for the citrullinated form. Ref.9

Subcellular location

Cytoplasm Ref.9.

Tissue specificity

Skin specific, specifically expressed at the inner endocuticle of hair fibers. Ref.8

Post-translational modification

More than half of the arginine residues undergo citrullination by PAD1 and PAD2. Arg-51 is specifically citrullinated by PAD3 and promotes tetramerization.

Sequence similarities

Belongs to the S-100 family.

Contains 2 EF-hand domains.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   DomainRepeat
   LigandCalcium
Metal-binding
Zinc
   PTMAcetylation
Citrullination
Disulfide bond
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from direct assay. Source: HPA

nucleolus

Inferred from direct assay. Source: HPA

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 101100Protein S100-A3
PRO_0000143972

Regions

Domain12 – 4736EF-hand 1
Domain50 – 8536EF-hand 2
Calcium binding20 – 33141; low affinity Potential
Calcium binding63 – 74122; high affinity Potential

Sites

Metal binding831Zinc
Metal binding861Zinc
Metal binding871Zinc
Metal binding931Zinc

Amino acid modifications

Modified residue21N-acetylalanine Ref.8
Modified residue511Citrulline; by PAD3
Disulfide bond30 ↔ 68 Ref.11
Disulfide bond81 ↔ 99 Ref.11

Natural variations

Natural variant31R → K.
Corresponds to variant rs36022742 [ dbSNP | Ensembl ].
VAR_061047

Experimental info

Mutagenesis301C → A: Abolishes calcium binding; when associated with Ala-68. Ref.11
Mutagenesis681C → A: Abolishes calcium binding; when associated with Ala-30. Ref.11
Mutagenesis811C → A: Increases affinity for calcium; when associated with Ala-99. Ref.11
Mutagenesis991C → A: Increases affinity for calcium; when associated with Ala-81. Ref.11

Secondary structure

................. 101
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P33764 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: ABCAF4B7E5F2E0A1

FASTA10111,713
        10         20         30         40         50         60 
MARPLEQAVA AIVCTFQEYA GRCGDKYKLC QAELKELLQK ELATWTPTEF RECDYNKFMS 

        70         80         90        100 
VLDTNKDCEV DFVEYVRSLA CLCLYCHEYF KDCPSEPPCS Q 

« Hide

References

« Hide 'large scale' references
[1]"Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E."
Engelkamp D., Schaefer B.W., Mattei M.-G., Erne P., Heizmann C.W.
Proc. Natl. Acad. Sci. U.S.A. 90:6547-6551(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[7]"Probing the structure of the human Ca2+- and Zn2+-binding protein S100A3: spectroscopic investigations of its transition metal ion complexes, and three-dimensional structural model."
Fritz G., Heizmann C.W., Kroneck P.M.H.
Biochim. Biophys. Acta 1448:264-276(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[8]"Characterization of the cysteine-rich calcium-binding S100A3 protein from human hair cuticles."
Kizawa K., Troxler H., Kleinert P., Inoue T., Toyoda M., Morohashi M., Heizmann C.W.
Biochem. Biophys. Res. Commun. 299:857-862(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, TISSUE SPECIFICITY.
[9]"Specific citrullination causes assembly of a globular S100A3 homotetramer: a putative Ca2+ modulator matures human hair cuticle."
Kizawa K., Takahara H., Troxler H., Kleinert P., Mochida U., Heizmann C.W.
J. Biol. Chem. 283:5004-5013(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, CITRULLINATION AT ARG-51, SUBCELLULAR LOCATION.
[10]"Metal-free MIRAS phasing: structure of apo-S100A3."
Mittl P.R., Fritz G., Sargent D.F., Richmond T.J., Heizmann C.W., Grutter M.G.
Acta Crystallogr. D 58:1255-1261(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
[11]"Refined crystal structures of human Ca(2+)/Zn(2+)-binding S100A3 protein characterized by two disulfide bridges."
Unno M., Kawasaki T., Takahara H., Heizmann C.W., Kizawa K.
J. Mol. Biol. 408:477-490(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS), DISULFIDE BONDS, ZINC-BINDING SITES, MUTAGENESIS OF CYS-30; CYS-68; CSY-81 AND CYS-99.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z18948 mRNA. Translation: CAA79471.1.
Z18950 Genomic DNA. Translation: CAA79473.1.
BT006955 mRNA. Translation: AAP35601.1.
CR542163 mRNA. Translation: CAG46960.1.
CR542185 mRNA. Translation: CAG46982.1.
BX470102 Genomic DNA. Translation: CAI14755.1.
CH471121 Genomic DNA. Translation: EAW53306.1.
CH471121 Genomic DNA. Translation: EAW53307.1.
BC012893 mRNA. Translation: AAH12893.1.
CCDSCCDS1043.1.
PIRC48219.
S70326.
RefSeqNP_002951.1. NM_002960.1.
UniGeneHs.557609.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KSOX-ray1.70A/B1-101[»]
3NSIX-ray2.15A/B1-101[»]
3NSKX-ray1.55A/B1-101[»]
3NSLX-ray1.50A/B/C/D/E/F2-101[»]
3NSOX-ray1.45A/B1-101[»]
ProteinModelPortalP33764.
SMRP33764. Positions 2-94.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112182. 2 interactions.
MINTMINT-4714713.
STRING9606.ENSP00000357701.

Polymorphism databases

DMDM464729.

Proteomic databases

MaxQBP33764.
PaxDbP33764.
PeptideAtlasP33764.
PRIDEP33764.

Protocols and materials databases

DNASU6274.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000368712; ENSP00000357701; ENSG00000188015.
ENST00000368713; ENSP00000357702; ENSG00000188015.
GeneID6274.
KEGGhsa:6274.
UCSCuc001fca.1. human.

Organism-specific databases

CTD6274.
GeneCardsGC01M153519.
H-InvDBHIX0116376.
HGNCHGNC:10493. S100A3.
HPAHPA042674.
MIM176992. gene.
neXtProtNX_P33764.
PharmGKBPA34905.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40471.
HOGENOMHOG000246968.
HOVERGENHBG001479.
InParanoidP33764.
OMACEVDFAE.
OrthoDBEOG779P19.
PhylomeDBP33764.
TreeFamTF332727.

Gene expression databases

BgeeP33764.
CleanExHS_S100A3.
GenevestigatorP33764.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
IPR028488. S100A3.
[Graphical view]
PANTHERPTHR11639:SF12. PTHR11639:SF12. 1 hit.
PfamPF01023. S_100. 1 hit.
[Graphical view]
PROSITEPS00303. S100_CABP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP33764.
GeneWikiS100A3.
GenomeRNAi6274.
NextBio24351.
PROP33764.
SOURCESearch...

Entry information

Entry nameS10A3_HUMAN
AccessionPrimary (citable) accession number: P33764
Secondary accession number(s): D3DV51, Q6FGE4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM