P33764 (S10A3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein S100-A3

Alternative name(s):
Protein S-100E
S100 calcium-binding protein A3

Gene names

Name:	S100A3
Synonyms:	S100E

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds both calcium and zinc. Probably binds 2 zinc ions per molecule. May be involved in calcium-dependent cuticle cell differentiation and hair shaft formation.
Subunit structure	Homodimer.
Tissue specificity	Skin specific.
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	Calcium Metal-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 101

101

Protein S100-A3

PRO_0000143972

Regions

Domain

12 – 47

36

EF-hand 1

Domain

50 – 85

36

EF-hand 2

Calcium binding

20 – 33

14

1; low affinity Potential

Calcium binding

63 – 74

12

2; high affinity Potential

Sites

Metal binding

81

1

Zinc 1 Potential

Metal binding

83

1

Zinc 1 Potential

Metal binding

83

1

Zinc 2 Potential

Metal binding

86

1

Zinc 1 Potential

Metal binding

86

1

Zinc 2 Potential

Metal binding

87

1

Zinc 1 Potential

Metal binding

93

1

Zinc 2 Potential

Metal binding

99

1

Zinc 2 Potential

Natural variations

Natural variant

3

1

R → K.
Corresponds to variant rs36022742 [ dbSNP | Ensembl ].

VAR_061047

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

101

Helix Strand Turn

Details...

Helix

4 – 20

17

Beta strand

22 – 24

3

Helix

31 – 41

11

Turn

42 – 44

3

Helix

52 – 64

13

Turn

65 – 67

3

Helix

72 – 86

15

Helix

88 – 90

3

Sequences

	Sequence		Length	Mass (Da)
	P33764 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: ABCAF4B7E5F2E0A1	FASTA	101	11,713
	10 20 30 40 50 60 MARPLEQAVA AIVCTFQEYA GRCGDKYKLC QAELKELLQK ELATWTPTEF RECDYNKFMS 70 80 90 100 VLDTNKDCEV DFVEYVRSLA CLCLYCHEYF KDCPSEPPCS Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E." Engelkamp D., Schaefer B.W., Mattei M.-G., Erne P., Heizmann C.W. Proc. Natl. Acad. Sci. U.S.A. 90:6547-6551(1993) [PubMed: 8341667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[7]	"Probing the structure of the human Ca2+- and Zn2+-binding protein S100A3: spectroscopic investigations of its transition metal ion complexes, and three-dimensional structural model." Fritz G., Heizmann C.W., Kroneck P.M.H. Biochim. Biophys. Acta 1448:264-276(1998) [PubMed: 9920417] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z18948 mRNA. Translation: CAA79471.1.
Z18950 Genomic DNA. Translation: CAA79473.1.
BT006955 mRNA. Translation: AAP35601.1.
CR542163 mRNA. Translation: CAG46960.1.
CR542185 mRNA. Translation: CAG46982.1.
BX470102 Genomic DNA. Translation: CAI14755.1.
CH471121 Genomic DNA. Translation: EAW53306.1.
CH471121 Genomic DNA. Translation: EAW53307.1.
BC012893 mRNA. Translation: AAH12893.1.

IPI

IPI00016752.

PIR

C48219.
S70326.

RefSeq

NP_002951.1. NM_002960.1.

UniGene

Hs.557609.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KSO	X-ray	1.70	A/B	1-101	[»]
3NSI	X-ray	2.15	A/B	1-101	[»]
3NSK	X-ray	1.55	A/B	1-101	[»]
3NSL	X-ray	1.50	A/B/C/D/E/F	2-101	[»]
3NSO	X-ray	1.45	A/B	1-101	[»]

ProteinModelPortal

P33764.

SMR

P33764. Positions 2-94.

ModBase

Search...

Protein-protein interaction databases

STRING

P33764.

Polymorphism databases

DMDM

464729.

Proteomic databases

PeptideAtlas

P33764.

PRIDE

P33764.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000368712; ENSP00000357701; ENSG00000188015.
ENST00000368713; ENSP00000357702; ENSG00000188015.

GeneID

6274.

KEGG

hsa:6274.

UCSC

uc001fca.1. human.

Organism-specific databases

CTD

6274.

GeneCards

GC01M153519.

H-InvDB

HIX0001082.
HIX0116376.

HGNC

HGNC:10493. S100A3.

HPA

HPA042674.

MIM

176992. gene.

neXtProt

NX_P33764.

PharmGKB

PA34905.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG21225.

GeneTree

ENSGT00600000084162.

HOGENOM

HBG716817.

HOVERGEN

HBG001479.

InParanoid

P33764.

OMA

GDKHKLC.

OrthoDB

EOG43210N.

PhylomeDB

P33764.

Gene expression databases

Bgee

P33764.

CleanEx

HS_S100A3.

Genevestigator

P33764.

GermOnline

ENSG00000188015. Homo sapiens.

Family and domain databases

InterPro

IPR011992. EF-hand-like_dom.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

Pfam

PF01023. S_100. 1 hit.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. False negative.
PS50222. EF_HAND_2. False negative.
PS00303. S100_CABP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

24351.

SOURCE

Search...

Entry information

Entry name

S10A3_HUMAN

Accession

Primary (citable) accession number: P33764
Secondary accession number(s): D3DV51, Q6FGE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.