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P33764

- S10A3_HUMAN

UniProt

P33764 - S10A3_HUMAN

Protein

Protein S100-A3

Gene

S100A3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds both calcium and zinc. May be involved in calcium-dependent cuticle cell differentiation, hair shaft and hair cuticular barrier formation.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi83 – 831Zinc
    Metal bindingi86 – 861Zinc
    Metal bindingi87 – 871Zinc
    Metal bindingi93 – 931Zinc

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi20 – 33141; low affinitySequence AnalysisAdd
    BLAST
    Calcium bindingi63 – 74122; high affinitySequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. zinc ion binding Source: InterPro

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein S100-A3
    Alternative name(s):
    Protein S-100E
    S100 calcium-binding protein A3
    Gene namesi
    Name:S100A3
    Synonyms:S100E
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:10493. S100A3.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. nucleolus Source: HPA

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi30 – 301C → A: Abolishes calcium binding; when associated with Ala-68. 1 Publication
    Mutagenesisi68 – 681C → A: Abolishes calcium binding; when associated with Ala-30. 1 Publication
    Mutagenesisi81 – 811C → A: Increases affinity for calcium; when associated with Ala-99. 1 Publication
    Mutagenesisi99 – 991C → A: Increases affinity for calcium; when associated with Ala-81. 1 Publication

    Organism-specific databases

    PharmGKBiPA34905.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 101100Protein S100-A3PRO_0000143972Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Disulfide bondi30 ↔ 681 Publication
    Modified residuei51 – 511Citrulline; by PAD31 Publication
    Disulfide bondi81 ↔ 991 Publication

    Post-translational modificationi

    More than half of the arginine residues undergo citrullination by PAD1 and PAD2. Arg-51 is specifically citrullinated by PAD3 and promotes tetramerization.1 Publication

    Keywords - PTMi

    Acetylation, Citrullination, Disulfide bond

    Proteomic databases

    MaxQBiP33764.
    PaxDbiP33764.
    PeptideAtlasiP33764.
    PRIDEiP33764.

    Expressioni

    Tissue specificityi

    Skin specific, specifically expressed at the inner endocuticle of hair fibers.1 Publication

    Gene expression databases

    BgeeiP33764.
    CleanExiHS_S100A3.
    GenevestigatoriP33764.

    Organism-specific databases

    HPAiHPA042674.

    Interactioni

    Subunit structurei

    Homodimer and homotetramer for the citrullinated form.1 Publication

    Protein-protein interaction databases

    BioGridi112182. 2 interactions.
    MINTiMINT-4714713.
    STRINGi9606.ENSP00000357701.

    Structurei

    Secondary structure

    1
    101
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 2017
    Beta strandi22 – 243
    Beta strandi28 – 303
    Helixi31 – 4111
    Turni42 – 443
    Turni49 – 513
    Helixi52 – 6413
    Turni65 – 673
    Beta strandi68 – 714
    Helixi72 – 8615
    Helixi88 – 903

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KSOX-ray1.70A/B1-101[»]
    3NSIX-ray2.15A/B1-101[»]
    3NSKX-ray1.55A/B1-101[»]
    3NSLX-ray1.50A/B/C/D/E/F2-101[»]
    3NSOX-ray1.45A/B1-101[»]
    ProteinModelPortaliP33764.
    SMRiP33764. Positions 2-94.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP33764.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 4736EF-hand 1Add
    BLAST
    Domaini50 – 8536EF-hand 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the S-100 family.Curated
    Contains 2 EF-hand domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG40471.
    HOGENOMiHOG000246968.
    HOVERGENiHBG001479.
    InParanoidiP33764.
    OMAiCEVDFAE.
    OrthoDBiEOG779P19.
    PhylomeDBiP33764.
    TreeFamiTF332727.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR001751. S100/CaBP-9k_CS.
    IPR013787. S100_Ca-bd_sub.
    IPR028488. S100A3.
    [Graphical view]
    PANTHERiPTHR11639:SF12. PTHR11639:SF12. 1 hit.
    PfamiPF01023. S_100. 1 hit.
    [Graphical view]
    PROSITEiPS00303. S100_CABP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33764-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARPLEQAVA AIVCTFQEYA GRCGDKYKLC QAELKELLQK ELATWTPTEF    50
    RECDYNKFMS VLDTNKDCEV DFVEYVRSLA CLCLYCHEYF KDCPSEPPCS 100
    Q 101
    Length:101
    Mass (Da):11,713
    Last modified:February 1, 1994 - v1
    Checksum:iABCAF4B7E5F2E0A1
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti3 – 31R → K.
    Corresponds to variant rs36022742 [ dbSNP | Ensembl ].
    VAR_061047

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z18948 mRNA. Translation: CAA79471.1.
    Z18950 Genomic DNA. Translation: CAA79473.1.
    BT006955 mRNA. Translation: AAP35601.1.
    CR542163 mRNA. Translation: CAG46960.1.
    CR542185 mRNA. Translation: CAG46982.1.
    BX470102 Genomic DNA. Translation: CAI14755.1.
    CH471121 Genomic DNA. Translation: EAW53306.1.
    CH471121 Genomic DNA. Translation: EAW53307.1.
    BC012893 mRNA. Translation: AAH12893.1.
    CCDSiCCDS1043.1.
    PIRiC48219.
    S70326.
    RefSeqiNP_002951.1. NM_002960.1.
    UniGeneiHs.557609.

    Genome annotation databases

    EnsembliENST00000368712; ENSP00000357701; ENSG00000188015.
    ENST00000368713; ENSP00000357702; ENSG00000188015.
    GeneIDi6274.
    KEGGihsa:6274.
    UCSCiuc001fca.1. human.

    Polymorphism databases

    DMDMi464729.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z18948 mRNA. Translation: CAA79471.1 .
    Z18950 Genomic DNA. Translation: CAA79473.1 .
    BT006955 mRNA. Translation: AAP35601.1 .
    CR542163 mRNA. Translation: CAG46960.1 .
    CR542185 mRNA. Translation: CAG46982.1 .
    BX470102 Genomic DNA. Translation: CAI14755.1 .
    CH471121 Genomic DNA. Translation: EAW53306.1 .
    CH471121 Genomic DNA. Translation: EAW53307.1 .
    BC012893 mRNA. Translation: AAH12893.1 .
    CCDSi CCDS1043.1.
    PIRi C48219.
    S70326.
    RefSeqi NP_002951.1. NM_002960.1.
    UniGenei Hs.557609.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KSO X-ray 1.70 A/B 1-101 [» ]
    3NSI X-ray 2.15 A/B 1-101 [» ]
    3NSK X-ray 1.55 A/B 1-101 [» ]
    3NSL X-ray 1.50 A/B/C/D/E/F 2-101 [» ]
    3NSO X-ray 1.45 A/B 1-101 [» ]
    ProteinModelPortali P33764.
    SMRi P33764. Positions 2-94.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112182. 2 interactions.
    MINTi MINT-4714713.
    STRINGi 9606.ENSP00000357701.

    Polymorphism databases

    DMDMi 464729.

    Proteomic databases

    MaxQBi P33764.
    PaxDbi P33764.
    PeptideAtlasi P33764.
    PRIDEi P33764.

    Protocols and materials databases

    DNASUi 6274.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000368712 ; ENSP00000357701 ; ENSG00000188015 .
    ENST00000368713 ; ENSP00000357702 ; ENSG00000188015 .
    GeneIDi 6274.
    KEGGi hsa:6274.
    UCSCi uc001fca.1. human.

    Organism-specific databases

    CTDi 6274.
    GeneCardsi GC01M153519.
    H-InvDB HIX0116376.
    HGNCi HGNC:10493. S100A3.
    HPAi HPA042674.
    MIMi 176992. gene.
    neXtProti NX_P33764.
    PharmGKBi PA34905.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG40471.
    HOGENOMi HOG000246968.
    HOVERGENi HBG001479.
    InParanoidi P33764.
    OMAi CEVDFAE.
    OrthoDBi EOG779P19.
    PhylomeDBi P33764.
    TreeFami TF332727.

    Miscellaneous databases

    EvolutionaryTracei P33764.
    GeneWikii S100A3.
    GenomeRNAii 6274.
    NextBioi 24351.
    PROi P33764.
    SOURCEi Search...

    Gene expression databases

    Bgeei P33764.
    CleanExi HS_S100A3.
    Genevestigatori P33764.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR001751. S100/CaBP-9k_CS.
    IPR013787. S100_Ca-bd_sub.
    IPR028488. S100A3.
    [Graphical view ]
    PANTHERi PTHR11639:SF12. PTHR11639:SF12. 1 hit.
    Pfami PF01023. S_100. 1 hit.
    [Graphical view ]
    PROSITEi PS00303. S100_CABP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E."
      Engelkamp D., Schaefer B.W., Mattei M.-G., Erne P., Heizmann C.W.
      Proc. Natl. Acad. Sci. U.S.A. 90:6547-6551(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    7. "Probing the structure of the human Ca2+- and Zn2+-binding protein S100A3: spectroscopic investigations of its transition metal ion complexes, and three-dimensional structural model."
      Fritz G., Heizmann C.W., Kroneck P.M.H.
      Biochim. Biophys. Acta 1448:264-276(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    8. "Characterization of the cysteine-rich calcium-binding S100A3 protein from human hair cuticles."
      Kizawa K., Troxler H., Kleinert P., Inoue T., Toyoda M., Morohashi M., Heizmann C.W.
      Biochem. Biophys. Res. Commun. 299:857-862(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, TISSUE SPECIFICITY.
    9. "Specific citrullination causes assembly of a globular S100A3 homotetramer: a putative Ca2+ modulator matures human hair cuticle."
      Kizawa K., Takahara H., Troxler H., Kleinert P., Mochida U., Heizmann C.W.
      J. Biol. Chem. 283:5004-5013(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, CITRULLINATION AT ARG-51, SUBCELLULAR LOCATION.
    10. Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
    11. "Refined crystal structures of human Ca(2+)/Zn(2+)-binding S100A3 protein characterized by two disulfide bridges."
      Unno M., Kawasaki T., Takahara H., Heizmann C.W., Kizawa K.
      J. Mol. Biol. 408:477-490(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS), DISULFIDE BONDS, ZINC-BINDING SITES, MUTAGENESIS OF CYS-30; CYS-68; CSY-81 AND CYS-99.

    Entry informationi

    Entry nameiS10A3_HUMAN
    AccessioniPrimary (citable) accession number: P33764
    Secondary accession number(s): D3DV51, Q6FGE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3