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Protein

Peroxisomal ATPase PEX6

Gene

PEX6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the peroxisomal protein import machinery. Together with PEX1, mediates the ATP-dependent relocation and recycling of the peroxisomal targeting signal-1 (PTS1) import receptor PEX5 from the peroxisomal membrane to the cytosol, where it is then available for another round of protein import into the organelle.3 Publications

Miscellaneous

Present with 1630 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi772 – 779ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • protein heterodimerization activity Source: SGD

GO - Biological processi

  • protein import into peroxisome matrix, receptor recycling Source: SGD
  • replicative cell aging Source: SGD

Keywordsi

Biological processPeroxisome biogenesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33313-MONOMER

Protein family/group databases

TCDBi3.A.20.1.5 the peroxisomal protein importer (ppi) family

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal ATPase PEX6
Alternative name(s):
Peroxin-6
Peroxisomal assembly protein 8
Peroxisome biosynthesis protein PAS8
Gene namesi
Name:PEX6
Synonyms:PAS8
Ordered Locus Names:YNL329C
ORF Names:N0310
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL329C
SGDiS000005273 PEX6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi489K → A in PEX6pA1; decreased binding to PEX15. 3 Publications1
Mutagenesisi778K → A in PEX6pA2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes. 3 Publications1
Mutagenesisi831D → Q in PEX6pB2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000846211 – 1030Peroxisomal ATPase PEX6Add BLAST1030

Proteomic databases

MaxQBiP33760
PaxDbiP33760
PRIDEiP33760

Interactioni

Subunit structurei

Interacts with PEX1; forms a high-molecular-mass heterooligomer in the cytosol. Interacts with PEX15; anchors PEX1-PEX6 heterooligomers to the peroxisomal membrane and mediates their association with the peroxisomal importomer.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein heterodimerization activity Source: SGD

Protein-protein interaction databases

BioGridi35512, 82 interactors
DIPiDIP-2621N
IntActiP33760, 15 interactors
MINTiP33760
STRINGi4932.YNL329C

Structurei

3D structure databases

ProteinModelPortaliP33760
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni478 – 683AAA-cassette D1Add BLAST206
Regioni767 – 956AAA-cassette D2Add BLAST190

Domaini

AAA-cassette D1 is required for interaction with PEX1. ATP-binding in AAA-cassette D1 is required for attachment to PEX15. ATP-binding and hydrolysis in AAA-cassette D2 is required for release from PEX15 and proper function in PEX5 dislocation.

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074953
HOGENOMiHOG000248247
InParanoidiP33760
KOiK13339
OMAiITSAMEN
OrthoDBiEOG092C0WBQ

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequencei

Sequence statusi: Complete.

P33760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKASLTFSLS GIYAPCSISR DIYLEYGDKK AECLYGTIRL PQYGPGCTPG
60 70 80 90 100
KIVHCVLDDS LPFCSIVVPS KLFGFMPTQP TMDFCYFEPI LDNVVPVLDS
110 120 130 140 150
VTFLINEQLY SKLMDLPQEM QQIQFLHYKY NINSMETVVH SRDILTSGLC
160 170 180 190 200
QILNCSPFPQ GLVDFTETQL ILVNDTEQKL SALKYANEDE EYALPKIGTN
210 220 230 240 250
SALSIDLESL PCTISRDLLR PAPHINDDNS IYAFTDAETL LRLDVTSGSF
260 270 280 290 300
ITVSNMGCVR LVKLFVLLLP NGFKKRTIYA PPKIIASFPD CSVVTISKSN
310 320 330 340 350
IGHTDIPIAN QVFISRVGGW LQSQKCFQNI ILTTLKKFFS ESKRILCQND
360 370 380 390 400
LIPIAFDSSM ADLNIAEEND ESDDEDELGQ YYKNDSLVWF FVTSAELDCF
410 420 430 440 450
SKDNSHFIID PNRTKLITTN ITNRRPLPLS RSNLQRYYGF AETFYYDLHI
460 470 480 490 500
FPYVRQLVNI LETSFNCSQR GITLNASVLL HSTTNNVGKA TMVRFASKYL
510 520 530 540 550
GIHLLEIDCL SLTSNSRQLD STSKIIGYIR AKCENVLPYA SPAVIFLAHL
560 570 580 590 600
DSILLDVNAN QDPEAIKLQK SINFEMSKLL DDFTFKFPGT TFVGSVNNID
610 620 630 640 650
NVPSSFRSHM RFEILVPVPS EAQRLRIFQW YLSSHELNRD VQQKVPVSYM
660 670 680 690 700
DNISFSSLSS YSAGLTPLDI KSIVETARMT ATARFYQESK KCGWLPQSIL
710 720 730 740 750
ITQEDLSKAT SKARNEFSVS IGAPQIPNVT WDDIGGIDFV KGEILDTIDM
760 770 780 790 800
PLKHPELFTS GMKKRSGILF YGPPGTGKTL MAKAIATNFS LNFFSVKGPE
810 820 830 840 850
LLNMYIGESE ANVRRVFQKA REAKPCVIFF DEIDSVAPKR GNQGDSGGVM
860 870 880 890 900
DRIVSQLLAE LDGMSTDADG VFVIGATNRP DLLDEALLRP GRFDKLLYLG
910 920 930 940 950
IPDTDTKQLN ILEALTRKFV LDNDVKLIEL AKLCPFNYTG ADFYALCSDA
960 970 980 990 1000
MLNAMSRIAR MVEKKVSQHN ELTGENISTR RWFDKIATKE DTKVVVKMED
1010 1020 1030
FLKAQEQLTP SVSRAELNHY EAVRANFEGA
Length:1,030
Mass (Da):115,571
Last modified:February 1, 1994 - v1
Checksum:i736FECB57BBFF8F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20789 Unassigned DNA Translation: AAA16574.1
Z46259 Genomic DNA Translation: CAA86369.1
Z71605 Genomic DNA Translation: CAA96261.1
X83226 Genomic DNA Translation: CAA58229.1
BK006947 Genomic DNA Translation: DAA10234.1
PIRiS43211
RefSeqiNP_014070.1, NM_001183167.1

Genome annotation databases

EnsemblFungiiYNL329C; YNL329C; YNL329C
GeneIDi855387
KEGGisce:YNL329C

Similar proteinsi

Entry informationi

Entry nameiPEX6_YEAST
AccessioniPrimary (citable) accession number: P33760
Secondary accession number(s): D6W0L8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health