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P33760

- PEX6_YEAST

UniProt

P33760 - PEX6_YEAST

Protein

Peroxisomal ATPase PEX6

Gene

PEX6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Component of the peroxisomal protein import machinery. Together with PEX1, mediates the ATP-dependent relocation and recycling of the peroxisomal targeting signal-1 (PTS1) import receptor PEX5 from the peroxisomal membrane to the cytosol, where it is then available for another round of protein import into the organelle.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi772 – 7798ATPSequence Analysis

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. protein heterodimerization activity Source: SGD

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. protein import into peroxisome matrix, receptor recycling Source: SGD
    3. replicative cell aging Source: SGD

    Keywords - Biological processi

    Peroxisome biogenesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33313-MONOMER.

    Protein family/group databases

    TCDBi3.A.20.1.5. the peroxisomal protein importer (ppi) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal ATPase PEX6
    Alternative name(s):
    Peroxin-6
    Peroxisomal assembly protein 8
    Peroxisome biosynthesis protein PAS8
    Gene namesi
    Name:PEX6
    Synonyms:PAS8
    Ordered Locus Names:YNL329C
    ORF Names:N0310
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL329c.
    SGDiS000005273. PEX6.

    Subcellular locationi

    Cytoplasm. Peroxisome membrane; Peripheral membrane protein; Cytoplasmic side
    Note: Shuttles between the cytoplasm and the peroxisomal membrane.

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. peroxisomal membrane Source: UniProtKB-SubCell
    3. peroxisome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Peroxisome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi489 – 4891K → A in PEX6pA1; decreased binding to PEX15. 3 Publications
    Mutagenesisi778 – 7781K → A in PEX6pA2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes. 3 Publications
    Mutagenesisi831 – 8311D → Q in PEX6pB2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes. 3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10301030Peroxisomal ATPase PEX6PRO_0000084621Add
    BLAST

    Proteomic databases

    MaxQBiP33760.
    PaxDbiP33760.
    PeptideAtlasiP33760.

    Expressioni

    Gene expression databases

    GenevestigatoriP33760.

    Interactioni

    Subunit structurei

    Interacts with PEX1; forms a high-molcular-mass heterooligomer in the cytosol. Interacts with PEX15; anchors PEX1-PEX6 heterooligomers to the peroxisomal membrane and mediates their association with the peroxisomal importomer.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PEX1P2400416EBI-13178,EBI-13155
    PEX14P531126EBI-13178,EBI-13212
    PEX15Q0821514EBI-13178,EBI-31849

    Protein-protein interaction databases

    BioGridi35512. 44 interactions.
    DIPiDIP-2621N.
    IntActiP33760. 10 interactions.
    MINTiMINT-423224.
    STRINGi4932.YNL329C.

    Structurei

    3D structure databases

    ProteinModelPortaliP33760.
    SMRiP33760. Positions 478-1014.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni478 – 683206AAA-cassette D1Add
    BLAST
    Regioni767 – 956190AAA-cassette D2Add
    BLAST

    Domaini

    AAA-cassette D1 is required for interaction with PEX1. ATP-binding in AAA-cassette D1 is required for attachment to PEX15. ATP-binding and hydrolysis in AAA-cassette D2 is required for release from PEX15 and proper function in PEX5 dislocation.

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Phylogenomic databases

    eggNOGiCOG0464.
    GeneTreeiENSGT00550000074953.
    HOGENOMiHOG000248247.
    KOiK13339.
    OMAiITSAMEN.
    OrthoDBiEOG7N37N0.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33760-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKASLTFSLS GIYAPCSISR DIYLEYGDKK AECLYGTIRL PQYGPGCTPG     50
    KIVHCVLDDS LPFCSIVVPS KLFGFMPTQP TMDFCYFEPI LDNVVPVLDS 100
    VTFLINEQLY SKLMDLPQEM QQIQFLHYKY NINSMETVVH SRDILTSGLC 150
    QILNCSPFPQ GLVDFTETQL ILVNDTEQKL SALKYANEDE EYALPKIGTN 200
    SALSIDLESL PCTISRDLLR PAPHINDDNS IYAFTDAETL LRLDVTSGSF 250
    ITVSNMGCVR LVKLFVLLLP NGFKKRTIYA PPKIIASFPD CSVVTISKSN 300
    IGHTDIPIAN QVFISRVGGW LQSQKCFQNI ILTTLKKFFS ESKRILCQND 350
    LIPIAFDSSM ADLNIAEEND ESDDEDELGQ YYKNDSLVWF FVTSAELDCF 400
    SKDNSHFIID PNRTKLITTN ITNRRPLPLS RSNLQRYYGF AETFYYDLHI 450
    FPYVRQLVNI LETSFNCSQR GITLNASVLL HSTTNNVGKA TMVRFASKYL 500
    GIHLLEIDCL SLTSNSRQLD STSKIIGYIR AKCENVLPYA SPAVIFLAHL 550
    DSILLDVNAN QDPEAIKLQK SINFEMSKLL DDFTFKFPGT TFVGSVNNID 600
    NVPSSFRSHM RFEILVPVPS EAQRLRIFQW YLSSHELNRD VQQKVPVSYM 650
    DNISFSSLSS YSAGLTPLDI KSIVETARMT ATARFYQESK KCGWLPQSIL 700
    ITQEDLSKAT SKARNEFSVS IGAPQIPNVT WDDIGGIDFV KGEILDTIDM 750
    PLKHPELFTS GMKKRSGILF YGPPGTGKTL MAKAIATNFS LNFFSVKGPE 800
    LLNMYIGESE ANVRRVFQKA REAKPCVIFF DEIDSVAPKR GNQGDSGGVM 850
    DRIVSQLLAE LDGMSTDADG VFVIGATNRP DLLDEALLRP GRFDKLLYLG 900
    IPDTDTKQLN ILEALTRKFV LDNDVKLIEL AKLCPFNYTG ADFYALCSDA 950
    MLNAMSRIAR MVEKKVSQHN ELTGENISTR RWFDKIATKE DTKVVVKMED 1000
    FLKAQEQLTP SVSRAELNHY EAVRANFEGA 1030
    Length:1,030
    Mass (Da):115,571
    Last modified:February 1, 1994 - v1
    Checksum:i736FECB57BBFF8F8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20789 Unassigned DNA. Translation: AAA16574.1.
    Z46259 Genomic DNA. Translation: CAA86369.1.
    Z71605 Genomic DNA. Translation: CAA96261.1.
    X83226 Genomic DNA. Translation: CAA58229.1.
    BK006947 Genomic DNA. Translation: DAA10234.1.
    PIRiS43211.
    RefSeqiNP_014070.1. NM_001183167.1.

    Genome annotation databases

    EnsemblFungiiYNL329C; YNL329C; YNL329C.
    GeneIDi855387.
    KEGGisce:YNL329C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20789 Unassigned DNA. Translation: AAA16574.1 .
    Z46259 Genomic DNA. Translation: CAA86369.1 .
    Z71605 Genomic DNA. Translation: CAA96261.1 .
    X83226 Genomic DNA. Translation: CAA58229.1 .
    BK006947 Genomic DNA. Translation: DAA10234.1 .
    PIRi S43211.
    RefSeqi NP_014070.1. NM_001183167.1.

    3D structure databases

    ProteinModelPortali P33760.
    SMRi P33760. Positions 478-1014.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35512. 44 interactions.
    DIPi DIP-2621N.
    IntActi P33760. 10 interactions.
    MINTi MINT-423224.
    STRINGi 4932.YNL329C.

    Protein family/group databases

    TCDBi 3.A.20.1.5. the peroxisomal protein importer (ppi) family.

    Proteomic databases

    MaxQBi P33760.
    PaxDbi P33760.
    PeptideAtlasi P33760.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL329C ; YNL329C ; YNL329C .
    GeneIDi 855387.
    KEGGi sce:YNL329C.

    Organism-specific databases

    CYGDi YNL329c.
    SGDi S000005273. PEX6.

    Phylogenomic databases

    eggNOGi COG0464.
    GeneTreei ENSGT00550000074953.
    HOGENOMi HOG000248247.
    KOi K13339.
    OMAi ITSAMEN.
    OrthoDBi EOG7N37N0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33313-MONOMER.

    Miscellaneous databases

    NextBioi 979190.
    PROi P33760.

    Gene expression databases

    Genevestigatori P33760.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 2 hits.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the PAS8 gene, the product of which is essential for biogenesis of peroxisomes in Saccharomyces cerevisiae."
      Voorn-Brouwer T., van der Leij I., Hemrika W., Distel B., Tabak H.F.
      Biochim. Biophys. Acta 1216:325-328(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
      Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
      Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "An 8.2 kb DNA segment from chromosome XIV carries the RPD3 and PAS8 genes as well as the Saccharomyces cerevisiae homologue of the thiamine-repressed nmt1 gene and a chromosome III-duplicated gene for a putative aryl-alcohol dehydrogenase."
      van Dyck L., Pascual-Ahuir A., Purnelle B., Goffeau A.
      Yeast 11:987-991(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 142-1030.
      Strain: ATCC 96604 / S288c / FY1679.
    6. "Pex15p of Saccharomyces cerevisiae provides a molecular basis for recruitment of the AAA peroxin Pex6p to peroxisomal membranes."
      Birschmann I., Stroobants A.K., van den Berg M., Schaefer A., Rosenkranz K., Kunau W.-H., Tabak H.F.
      Mol. Biol. Cell 14:2226-2236(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PEX15, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-489; LYS-778 AND ASP-831.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p."
      Birschmann I., Rosenkranz K., Erdmann R., Kunau W.-H.
      FEBS J. 272:47-58(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PEX1, MUTAGENESIS OF LYS-489; LYS-778 AND ASP-831.
    10. "Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol."
      Platta H.W., Grunau S., Rosenkranz K., Girzalsky W., Erdmann R.
      Nat. Cell Biol. 7:817-822(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PEX1, MUTAGENESIS OF LYS-489; LYS-778 AND ASP-831.
    11. "Functional association of the AAA complex and the peroxisomal importomer."
      Rosenkranz K., Birschmann I., Grunau S., Girzalsky W., Kunau W.-H., Erdmann R.
      FEBS J. 273:3804-3815(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPEX6_YEAST
    AccessioniPrimary (citable) accession number: P33760
    Secondary accession number(s): D6W0L8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1630 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3