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P33760 (PEX6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peroxisomal ATPase PEX6
Alternative name(s):
Peroxin-6
Peroxisomal assembly protein 8
Peroxisome biosynthesis protein PAS8
Gene names
Name:PEX6
Synonyms:PAS8
Ordered Locus Names:YNL329C
ORF Names:N0310
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1030 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the peroxisomal protein import machinery. Together with PEX1, mediates the ATP-dependent relocation and recycling of the peroxisomal targeting signal-1 (PTS1) import receptor PEX5 from the peroxisomal membrane to the cytosol, where it is then available for another round of protein import into the organelle. Ref.9 Ref.10 Ref.11

Subunit structure

Interacts with PEX1; forms a high-molcular-mass heterooligomer in the cytosol. Interacts with PEX15; anchors PEX1-PEX6 heterooligomers to the peroxisomal membrane and mediates their association with the peroxisomal importomer. Ref.6 Ref.9 Ref.10

Subcellular location

Cytoplasm. Peroxisome membrane; Peripheral membrane protein; Cytoplasmic side. Note: Shuttles between the cytoplasm and the peroxisomal membrane. Ref.6 Ref.7

Domain

AAA-cassette D1 is required for interaction with PEX1. ATP-binding in AAA-cassette D1 is required for attachment to PEX15. ATP-binding and hydrolysis in AAA-cassette D2 is required for release from PEX15 and proper function in PEX5 dislocation.

Miscellaneous

Present with 1630 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the AAA ATPase family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10301030Peroxisomal ATPase PEX6
PRO_0000084621

Regions

Nucleotide binding772 – 7798ATP Potential
Region478 – 683206AAA-cassette D1
Region767 – 956190AAA-cassette D2

Experimental info

Mutagenesis4891K → A in PEX6pA1; decreased binding to PEX15. Ref.6 Ref.9 Ref.10
Mutagenesis7781K → A in PEX6pA2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes. Ref.6 Ref.9 Ref.10
Mutagenesis8311D → Q in PEX6pB2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes. Ref.6 Ref.9 Ref.10

Sequences

Sequence LengthMass (Da)Tools
P33760 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 736FECB57BBFF8F8

FASTA1,030115,571
        10         20         30         40         50         60 
MKASLTFSLS GIYAPCSISR DIYLEYGDKK AECLYGTIRL PQYGPGCTPG KIVHCVLDDS 

        70         80         90        100        110        120 
LPFCSIVVPS KLFGFMPTQP TMDFCYFEPI LDNVVPVLDS VTFLINEQLY SKLMDLPQEM 

       130        140        150        160        170        180 
QQIQFLHYKY NINSMETVVH SRDILTSGLC QILNCSPFPQ GLVDFTETQL ILVNDTEQKL 

       190        200        210        220        230        240 
SALKYANEDE EYALPKIGTN SALSIDLESL PCTISRDLLR PAPHINDDNS IYAFTDAETL 

       250        260        270        280        290        300 
LRLDVTSGSF ITVSNMGCVR LVKLFVLLLP NGFKKRTIYA PPKIIASFPD CSVVTISKSN 

       310        320        330        340        350        360 
IGHTDIPIAN QVFISRVGGW LQSQKCFQNI ILTTLKKFFS ESKRILCQND LIPIAFDSSM 

       370        380        390        400        410        420 
ADLNIAEEND ESDDEDELGQ YYKNDSLVWF FVTSAELDCF SKDNSHFIID PNRTKLITTN 

       430        440        450        460        470        480 
ITNRRPLPLS RSNLQRYYGF AETFYYDLHI FPYVRQLVNI LETSFNCSQR GITLNASVLL 

       490        500        510        520        530        540 
HSTTNNVGKA TMVRFASKYL GIHLLEIDCL SLTSNSRQLD STSKIIGYIR AKCENVLPYA 

       550        560        570        580        590        600 
SPAVIFLAHL DSILLDVNAN QDPEAIKLQK SINFEMSKLL DDFTFKFPGT TFVGSVNNID 

       610        620        630        640        650        660 
NVPSSFRSHM RFEILVPVPS EAQRLRIFQW YLSSHELNRD VQQKVPVSYM DNISFSSLSS 

       670        680        690        700        710        720 
YSAGLTPLDI KSIVETARMT ATARFYQESK KCGWLPQSIL ITQEDLSKAT SKARNEFSVS 

       730        740        750        760        770        780 
IGAPQIPNVT WDDIGGIDFV KGEILDTIDM PLKHPELFTS GMKKRSGILF YGPPGTGKTL 

       790        800        810        820        830        840 
MAKAIATNFS LNFFSVKGPE LLNMYIGESE ANVRRVFQKA REAKPCVIFF DEIDSVAPKR 

       850        860        870        880        890        900 
GNQGDSGGVM DRIVSQLLAE LDGMSTDADG VFVIGATNRP DLLDEALLRP GRFDKLLYLG 

       910        920        930        940        950        960 
IPDTDTKQLN ILEALTRKFV LDNDVKLIEL AKLCPFNYTG ADFYALCSDA MLNAMSRIAR 

       970        980        990       1000       1010       1020 
MVEKKVSQHN ELTGENISTR RWFDKIATKE DTKVVVKMED FLKAQEQLTP SVSRAELNHY 

      1030 
EAVRANFEGA 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the PAS8 gene, the product of which is essential for biogenesis of peroxisomes in Saccharomyces cerevisiae."
Voorn-Brouwer T., van der Leij I., Hemrika W., Distel B., Tabak H.F.
Biochim. Biophys. Acta 1216:325-328(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1676.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"An 8.2 kb DNA segment from chromosome XIV carries the RPD3 and PAS8 genes as well as the Saccharomyces cerevisiae homologue of the thiamine-repressed nmt1 gene and a chromosome III-duplicated gene for a putative aryl-alcohol dehydrogenase."
van Dyck L., Pascual-Ahuir A., Purnelle B., Goffeau A.
Yeast 11:987-991(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 142-1030.
Strain: ATCC 96604 / S288c / FY1679.
[6]"Pex15p of Saccharomyces cerevisiae provides a molecular basis for recruitment of the AAA peroxin Pex6p to peroxisomal membranes."
Birschmann I., Stroobants A.K., van den Berg M., Schaefer A., Rosenkranz K., Kunau W.-H., Tabak H.F.
Mol. Biol. Cell 14:2226-2236(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PEX15, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-489; LYS-778 AND ASP-831.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p."
Birschmann I., Rosenkranz K., Erdmann R., Kunau W.-H.
FEBS J. 272:47-58(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PEX1, MUTAGENESIS OF LYS-489; LYS-778 AND ASP-831.
[10]"Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol."
Platta H.W., Grunau S., Rosenkranz K., Girzalsky W., Erdmann R.
Nat. Cell Biol. 7:817-822(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PEX1, MUTAGENESIS OF LYS-489; LYS-778 AND ASP-831.
[11]"Functional association of the AAA complex and the peroxisomal importomer."
Rosenkranz K., Birschmann I., Grunau S., Girzalsky W., Kunau W.-H., Erdmann R.
FEBS J. 273:3804-3815(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L20789 Unassigned DNA. Translation: AAA16574.1.
Z46259 Genomic DNA. Translation: CAA86369.1.
Z71605 Genomic DNA. Translation: CAA96261.1.
X83226 Genomic DNA. Translation: CAA58229.1.
BK006947 Genomic DNA. Translation: DAA10234.1.
PIRS43211.
RefSeqNP_014070.1. NM_001183167.1.

3D structure databases

ProteinModelPortalP33760.
SMRP33760. Positions 478-1014.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35512. 43 interactions.
DIPDIP-2621N.
IntActP33760. 10 interactions.
MINTMINT-423224.
STRING4932.YNL329C.

Protein family/group databases

TCDB3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Proteomic databases

MaxQBP33760.
PaxDbP33760.
PeptideAtlasP33760.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL329C; YNL329C; YNL329C.
GeneID855387.
KEGGsce:YNL329C.

Organism-specific databases

CYGDYNL329c.
SGDS000005273. PEX6.

Phylogenomic databases

eggNOGCOG0464.
GeneTreeENSGT00550000074953.
HOGENOMHOG000248247.
KOK13339.
OMAITSAMEN.
OrthoDBEOG7N37N0.

Enzyme and pathway databases

BioCycYEAST:G3O-33313-MONOMER.

Gene expression databases

GenevestigatorP33760.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00004. AAA. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979190.
PROP33760.

Entry information

Entry namePEX6_YEAST
AccessionPrimary (citable) accession number: P33760
Secondary accession number(s): D6W0L8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families