P33760 (PEX6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal ATPase PEX6

Alternative name(s):
Peroxin-6
Peroxisomal assembly protein 8
Peroxisome biosynthesis protein PAS8

Gene names

Name:	PEX6
Synonyms:	PAS8
Ordered Locus Names:	YNL329C
ORF Names:	N0310

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1030 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the peroxisomal protein import machinery. Together with PEX1, mediates the ATP-dependent relocation and recycling of the peroxisomal targeting signal-1 (PTS1) import receptor PEX5 from the peroxisomal membrane to the cytosol, where it is then available for another round of protein import into the organelle. Ref.9 Ref.10 Ref.11
Subunit structure	Interacts with PEX1; forms a high-molcular-mass heterooligomer in the cytosol. Interacts with PEX15; anchors PEX1-PEX6 heterooligomers to the peroxisomal membrane and mediates their association with the peroxisomal importomer. Ref.6 Ref.9 Ref.10
Subcellular location	Cytoplasm. Peroxisome membrane; Peripheral membrane protein; Cytoplasmic side. Note: Shuttles between the cytoplasm and the peroxisomal membrane. Ref.6 Ref.7
Domain	AAA-cassette D1 is required for interaction with PEX1. ATP-binding in AAA-cassette D1 is required for attachment to PEX15. ATP-binding and hydrolysis in AAA-cassette D2 is required for release from PEX15 and proper function in PEX5 dislocation.
Miscellaneous	Present with 1630 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the AAA ATPase family.

Ontologies

Keywords
Biological process	Peroxisome biogenesis
Cellular component	Cytoplasm Membrane Peroxisome
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein import into peroxisome matrix, receptor recycling Inferred from mutant phenotype Ref.10. Source: SGD replicative cell aging Inferred from genetic interaction. Source: SGD
Cellular component	cytosol Inferred from direct assay Ref.6. Source: SGD membrane fraction Inferred from direct assay Ref.11. Source: SGD peroxisomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell soluble fraction Inferred from direct assay Ref.6Ref.11. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from mutant phenotype Ref.9. Source: SGD protein heterodimerization activity Inferred from mutant phenotype Ref.9. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PEX1	P24004	3	EBI-13178,EBI-13155

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1030	1030	Peroxisomal ATPase PEX6		PRO_0000084621
Regions
	Nucleotide binding	772 – 779	8	ATP Potential
	Region	478 – 683	206	AAA-cassette D1
	Region	767 – 956	190	AAA-cassette D2
Experimental info
	Mutagenesis	489	1	K → A in PEX6pA1; decreased binding to PEX15. Ref.6 Ref.9 Ref.10
	Mutagenesis	778	1	K → A in PEX6pA2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes. Ref.6 Ref.9 Ref.10
	Mutagenesis	831	1	D → Q in PEX6pB2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes. Ref.6 Ref.9 Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P33760 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 736FECB57BBFF8F8	FASTA	1,030	115,571
	10 20 30 40 50 60 MKASLTFSLS GIYAPCSISR DIYLEYGDKK AECLYGTIRL PQYGPGCTPG KIVHCVLDDS 70 80 90 100 110 120 LPFCSIVVPS KLFGFMPTQP TMDFCYFEPI LDNVVPVLDS VTFLINEQLY SKLMDLPQEM 130 140 150 160 170 180 QQIQFLHYKY NINSMETVVH SRDILTSGLC QILNCSPFPQ GLVDFTETQL ILVNDTEQKL 190 200 210 220 230 240 SALKYANEDE EYALPKIGTN SALSIDLESL PCTISRDLLR PAPHINDDNS IYAFTDAETL 250 260 270 280 290 300 LRLDVTSGSF ITVSNMGCVR LVKLFVLLLP NGFKKRTIYA PPKIIASFPD CSVVTISKSN 310 320 330 340 350 360 IGHTDIPIAN QVFISRVGGW LQSQKCFQNI ILTTLKKFFS ESKRILCQND LIPIAFDSSM 370 380 390 400 410 420 ADLNIAEEND ESDDEDELGQ YYKNDSLVWF FVTSAELDCF SKDNSHFIID PNRTKLITTN 430 440 450 460 470 480 ITNRRPLPLS RSNLQRYYGF AETFYYDLHI FPYVRQLVNI LETSFNCSQR GITLNASVLL 490 500 510 520 530 540 HSTTNNVGKA TMVRFASKYL GIHLLEIDCL SLTSNSRQLD STSKIIGYIR AKCENVLPYA 550 560 570 580 590 600 SPAVIFLAHL DSILLDVNAN QDPEAIKLQK SINFEMSKLL DDFTFKFPGT TFVGSVNNID 610 620 630 640 650 660 NVPSSFRSHM RFEILVPVPS EAQRLRIFQW YLSSHELNRD VQQKVPVSYM DNISFSSLSS 670 680 690 700 710 720 YSAGLTPLDI KSIVETARMT ATARFYQESK KCGWLPQSIL ITQEDLSKAT SKARNEFSVS 730 740 750 760 770 780 IGAPQIPNVT WDDIGGIDFV KGEILDTIDM PLKHPELFTS GMKKRSGILF YGPPGTGKTL 790 800 810 820 830 840 MAKAIATNFS LNFFSVKGPE LLNMYIGESE ANVRRVFQKA REAKPCVIFF DEIDSVAPKR 850 860 870 880 890 900 GNQGDSGGVM DRIVSQLLAE LDGMSTDADG VFVIGATNRP DLLDEALLRP GRFDKLLYLG 910 920 930 940 950 960 IPDTDTKQLN ILEALTRKFV LDNDVKLIEL AKLCPFNYTG ADFYALCSDA MLNAMSRIAR 970 980 990 1000 1010 1020 MVEKKVSQHN ELTGENISTR RWFDKIATKE DTKVVVKMED FLKAQEQLTP SVSRAELNHY 1030 EAVRANFEGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the PAS8 gene, the product of which is essential for biogenesis of peroxisomes in Saccharomyces cerevisiae." Voorn-Brouwer T., van der Leij I., Hemrika W., Distel B., Tabak H.F. Biochim. Biophys. Acta 1216:325-328(1993) [PubMed: 8241279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:567-572(1995) [PubMed: 7645347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"An 8.2 kb DNA segment from chromosome XIV carries the RPD3 and PAS8 genes as well as the Saccharomyces cerevisiae homologue of the thiamine-repressed nmt1 gene and a chromosome III-duplicated gene for a putative aryl-alcohol dehydrogenase." van Dyck L., Pascual-Ahuir A., Purnelle B., Goffeau A. Yeast 11:987-991(1995) [PubMed: 8533474] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 142-1030. Strain: ATCC 96604 / S288c / FY1679.
[6]	"Pex15p of Saccharomyces cerevisiae provides a molecular basis for recruitment of the AAA peroxin Pex6p to peroxisomal membranes." Birschmann I., Stroobants A.K., van den Berg M., Schaefer A., Rosenkranz K., Kunau W.-H., Tabak H.F. Mol. Biol. Cell 14:2226-2236(2003) [PubMed: 12808025] [Abstract] Cited for: INTERACTION WITH PEX15, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-489; LYS-778 AND ASP-831.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p." Birschmann I., Rosenkranz K., Erdmann R., Kunau W.-H. FEBS J. 272:47-58(2005) [PubMed: 15634331] [Abstract] Cited for: FUNCTION, INTERACTION WITH PEX1, MUTAGENESIS OF LYS-489; LYS-778 AND ASP-831.
[10]	"Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol." Platta H.W., Grunau S., Rosenkranz K., Girzalsky W., Erdmann R. Nat. Cell Biol. 7:817-822(2005) [PubMed: 16007078] [Abstract] Cited for: FUNCTION, INTERACTION WITH PEX1, MUTAGENESIS OF LYS-489; LYS-778 AND ASP-831.
[11]	"Functional association of the AAA complex and the peroxisomal importomer." Rosenkranz K., Birschmann I., Grunau S., Girzalsky W., Kunau W.-H., Erdmann R. FEBS J. 273:3804-3815(2006) [PubMed: 16911527] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L20789 Unassigned DNA. Translation: AAA16574.1. Z46259 Genomic DNA. Translation: CAA86369.1. Z71605 Genomic DNA. Translation: CAA96261.1. X83226 Genomic DNA. Translation: CAA58229.1. BK006947 Genomic DNA. Translation: DAA10234.1.
PIR	S43211.
RefSeq	NP_014070.1. NM_001183167.1.
3D structure databases
ProteinModelPortal	P33760.
SMR	P33760. Positions 476-501, 589-617, 723-1010.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2621N.
IntAct	P33760. 4 interactions.
MINT	MINT-423224.
STRING	P33760.
Proteomic databases
PeptideAtlas	P33760.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL329C; YNL329C; YNL329C.
GeneID	855387.
KEGG	sce:YNL329C.
NMPDR	fig|4932.3.peg.5132.
Organism-specific databases
CYGD	YNL329c.
SGD	S000005273. PEX6.
Phylogenomic databases
eggNOG	fuNOG06798.
HOGENOM	HBG396289.
OMA	FTGADFY.
OrthoDB	EOG48PQTF.
Gene expression databases
ArrayExpress	P33760.
Genevestigator	P33760.
GermOnline	YNL329C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. [Graphical view]
KO	K13339.
Pfam	PF00004. AAA. 2 hits. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979190.

Entry information

Entry name

PEX6_YEAST

Accession

Primary (citable) accession number: P33760
Secondary accession number(s): D6W0L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program