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P33760

- PEX6_YEAST

UniProt

P33760 - PEX6_YEAST

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Protein

Peroxisomal ATPase PEX6

Gene
PEX6, PAS8, YNL329C, N0310
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the peroxisomal protein import machinery. Together with PEX1, mediates the ATP-dependent relocation and recycling of the peroxisomal targeting signal-1 (PTS1) import receptor PEX5 from the peroxisomal membrane to the cytosol, where it is then available for another round of protein import into the organelle.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi772 – 7798ATP Reviewed prediction

GO - Molecular functioni

  1. ATPase activity Source: SGD
  2. ATP binding Source: UniProtKB-KW
  3. protein binding Source: IntAct
  4. protein heterodimerization activity Source: SGD

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. protein import into peroxisome matrix, receptor recycling Source: SGD
  3. replicative cell aging Source: SGD
Complete GO annotation...

Keywords - Biological processi

Peroxisome biogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33313-MONOMER.

Protein family/group databases

TCDBi3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal ATPase PEX6
Alternative name(s):
Peroxin-6
Peroxisomal assembly protein 8
Peroxisome biosynthesis protein PAS8
Gene namesi
Name:PEX6
Synonyms:PAS8
Ordered Locus Names:YNL329C
ORF Names:N0310
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL329c.
SGDiS000005273. PEX6.

Subcellular locationi

Cytoplasm. Peroxisome membrane; Peripheral membrane protein; Cytoplasmic side
Note: Shuttles between the cytoplasm and the peroxisomal membrane.2 Publications

GO - Cellular componenti

  1. cytosol Source: SGD
  2. peroxisomal membrane Source: UniProtKB-SubCell
  3. peroxisome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi489 – 4891K → A in PEX6pA1; decreased binding to PEX15. 3 Publications
Mutagenesisi778 – 7781K → A in PEX6pA2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes. 3 Publications
Mutagenesisi831 – 8311D → Q in PEX6pB2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes. 3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10301030Peroxisomal ATPase PEX6PRO_0000084621Add
BLAST

Proteomic databases

MaxQBiP33760.
PaxDbiP33760.
PeptideAtlasiP33760.

Expressioni

Gene expression databases

GenevestigatoriP33760.

Interactioni

Subunit structurei

Interacts with PEX1; forms a high-molcular-mass heterooligomer in the cytosol. Interacts with PEX15; anchors PEX1-PEX6 heterooligomers to the peroxisomal membrane and mediates their association with the peroxisomal importomer.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PEX1P2400416EBI-13178,EBI-13155
PEX14P531126EBI-13178,EBI-13212
PEX15Q0821514EBI-13178,EBI-31849

Protein-protein interaction databases

BioGridi35512. 44 interactions.
DIPiDIP-2621N.
IntActiP33760. 10 interactions.
MINTiMINT-423224.
STRINGi4932.YNL329C.

Structurei

3D structure databases

ProteinModelPortaliP33760.
SMRiP33760. Positions 478-1014.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni478 – 683206AAA-cassette D1Add
BLAST
Regioni767 – 956190AAA-cassette D2Add
BLAST

Domaini

AAA-cassette D1 is required for interaction with PEX1. ATP-binding in AAA-cassette D1 is required for attachment to PEX15. ATP-binding and hydrolysis in AAA-cassette D2 is required for release from PEX15 and proper function in PEX5 dislocation.

Sequence similaritiesi

Belongs to the AAA ATPase family.

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00550000074953.
HOGENOMiHOG000248247.
KOiK13339.
OMAiITSAMEN.
OrthoDBiEOG7N37N0.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33760-1 [UniParc]FASTAAdd to Basket

« Hide

MKASLTFSLS GIYAPCSISR DIYLEYGDKK AECLYGTIRL PQYGPGCTPG     50
KIVHCVLDDS LPFCSIVVPS KLFGFMPTQP TMDFCYFEPI LDNVVPVLDS 100
VTFLINEQLY SKLMDLPQEM QQIQFLHYKY NINSMETVVH SRDILTSGLC 150
QILNCSPFPQ GLVDFTETQL ILVNDTEQKL SALKYANEDE EYALPKIGTN 200
SALSIDLESL PCTISRDLLR PAPHINDDNS IYAFTDAETL LRLDVTSGSF 250
ITVSNMGCVR LVKLFVLLLP NGFKKRTIYA PPKIIASFPD CSVVTISKSN 300
IGHTDIPIAN QVFISRVGGW LQSQKCFQNI ILTTLKKFFS ESKRILCQND 350
LIPIAFDSSM ADLNIAEEND ESDDEDELGQ YYKNDSLVWF FVTSAELDCF 400
SKDNSHFIID PNRTKLITTN ITNRRPLPLS RSNLQRYYGF AETFYYDLHI 450
FPYVRQLVNI LETSFNCSQR GITLNASVLL HSTTNNVGKA TMVRFASKYL 500
GIHLLEIDCL SLTSNSRQLD STSKIIGYIR AKCENVLPYA SPAVIFLAHL 550
DSILLDVNAN QDPEAIKLQK SINFEMSKLL DDFTFKFPGT TFVGSVNNID 600
NVPSSFRSHM RFEILVPVPS EAQRLRIFQW YLSSHELNRD VQQKVPVSYM 650
DNISFSSLSS YSAGLTPLDI KSIVETARMT ATARFYQESK KCGWLPQSIL 700
ITQEDLSKAT SKARNEFSVS IGAPQIPNVT WDDIGGIDFV KGEILDTIDM 750
PLKHPELFTS GMKKRSGILF YGPPGTGKTL MAKAIATNFS LNFFSVKGPE 800
LLNMYIGESE ANVRRVFQKA REAKPCVIFF DEIDSVAPKR GNQGDSGGVM 850
DRIVSQLLAE LDGMSTDADG VFVIGATNRP DLLDEALLRP GRFDKLLYLG 900
IPDTDTKQLN ILEALTRKFV LDNDVKLIEL AKLCPFNYTG ADFYALCSDA 950
MLNAMSRIAR MVEKKVSQHN ELTGENISTR RWFDKIATKE DTKVVVKMED 1000
FLKAQEQLTP SVSRAELNHY EAVRANFEGA 1030
Length:1,030
Mass (Da):115,571
Last modified:February 1, 1994 - v1
Checksum:i736FECB57BBFF8F8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L20789 Unassigned DNA. Translation: AAA16574.1.
Z46259 Genomic DNA. Translation: CAA86369.1.
Z71605 Genomic DNA. Translation: CAA96261.1.
X83226 Genomic DNA. Translation: CAA58229.1.
BK006947 Genomic DNA. Translation: DAA10234.1.
PIRiS43211.
RefSeqiNP_014070.1. NM_001183167.1.

Genome annotation databases

EnsemblFungiiYNL329C; YNL329C; YNL329C.
GeneIDi855387.
KEGGisce:YNL329C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L20789 Unassigned DNA. Translation: AAA16574.1 .
Z46259 Genomic DNA. Translation: CAA86369.1 .
Z71605 Genomic DNA. Translation: CAA96261.1 .
X83226 Genomic DNA. Translation: CAA58229.1 .
BK006947 Genomic DNA. Translation: DAA10234.1 .
PIRi S43211.
RefSeqi NP_014070.1. NM_001183167.1.

3D structure databases

ProteinModelPortali P33760.
SMRi P33760. Positions 478-1014.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35512. 44 interactions.
DIPi DIP-2621N.
IntActi P33760. 10 interactions.
MINTi MINT-423224.
STRINGi 4932.YNL329C.

Protein family/group databases

TCDBi 3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Proteomic databases

MaxQBi P33760.
PaxDbi P33760.
PeptideAtlasi P33760.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL329C ; YNL329C ; YNL329C .
GeneIDi 855387.
KEGGi sce:YNL329C.

Organism-specific databases

CYGDi YNL329c.
SGDi S000005273. PEX6.

Phylogenomic databases

eggNOGi COG0464.
GeneTreei ENSGT00550000074953.
HOGENOMi HOG000248247.
KOi K13339.
OMAi ITSAMEN.
OrthoDBi EOG7N37N0.

Enzyme and pathway databases

BioCyci YEAST:G3O-33313-MONOMER.

Miscellaneous databases

NextBioi 979190.
PROi P33760.

Gene expression databases

Genevestigatori P33760.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 2 hits.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00674. AAA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the PAS8 gene, the product of which is essential for biogenesis of peroxisomes in Saccharomyces cerevisiae."
    Voorn-Brouwer T., van der Leij I., Hemrika W., Distel B., Tabak H.F.
    Biochim. Biophys. Acta 1216:325-328(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
    Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
    Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "An 8.2 kb DNA segment from chromosome XIV carries the RPD3 and PAS8 genes as well as the Saccharomyces cerevisiae homologue of the thiamine-repressed nmt1 gene and a chromosome III-duplicated gene for a putative aryl-alcohol dehydrogenase."
    van Dyck L., Pascual-Ahuir A., Purnelle B., Goffeau A.
    Yeast 11:987-991(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 142-1030.
    Strain: ATCC 96604 / S288c / FY1679.
  6. "Pex15p of Saccharomyces cerevisiae provides a molecular basis for recruitment of the AAA peroxin Pex6p to peroxisomal membranes."
    Birschmann I., Stroobants A.K., van den Berg M., Schaefer A., Rosenkranz K., Kunau W.-H., Tabak H.F.
    Mol. Biol. Cell 14:2226-2236(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PEX15, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-489; LYS-778 AND ASP-831.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p."
    Birschmann I., Rosenkranz K., Erdmann R., Kunau W.-H.
    FEBS J. 272:47-58(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PEX1, MUTAGENESIS OF LYS-489; LYS-778 AND ASP-831.
  10. "Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol."
    Platta H.W., Grunau S., Rosenkranz K., Girzalsky W., Erdmann R.
    Nat. Cell Biol. 7:817-822(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PEX1, MUTAGENESIS OF LYS-489; LYS-778 AND ASP-831.
  11. "Functional association of the AAA complex and the peroxisomal importomer."
    Rosenkranz K., Birschmann I., Grunau S., Girzalsky W., Kunau W.-H., Erdmann R.
    FEBS J. 273:3804-3815(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPEX6_YEAST
AccessioniPrimary (citable) accession number: P33760
Secondary accession number(s): D6W0L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1630 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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