Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (uracil(54)-C(5))-methyltransferase

Gene

TRM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation.2 Publications

Caution

Was originally thought to be an endo-exonuclease.1 Publication
It is uncertain whether Met-1, Met-38, Met-71, Met-77 or Met-82 is the initiator.Curated

Catalytic activityi

S-adenosyl-L-methionine + uracil(54) in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil(54) in tRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei461S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei496S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei517S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei564S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei591NucleophilePROSITE-ProRule annotation1
Active sitei631Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • tRNA modification Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-32025-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (uracil(54)-C(5))-methyltransferase (EC:2.1.1.35)
Alternative name(s):
Transfer RNA methyltransferase 2
tRNA(m5U54)-methyltransferase
Gene namesi
Name:TRM2
Synonyms:NUC2, NUD1, RNC1
Ordered Locus Names:YKR056W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR056W
SGDiS000001764 TRM2

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi498G → D: Unable to methylate tRNA. 1 Publication1
Mutagenesisi591C → A: Unable to methylate tRNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001620591 – 639tRNA (uracil(54)-C(5))-methyltransferaseAdd BLAST639

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei92PhosphoserineCombined sources1
Modified residuei93PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33753
PaxDbiP33753
PRIDEiP33753

PTM databases

iPTMnetiP33753

Interactioni

Protein-protein interaction databases

BioGridi34187, 96 interactors
MINTiP33753
STRINGi4932.YKR056W

Structurei

3D structure databases

ProteinModelPortaliP33753
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini163 – 228TRAMPROSITE-ProRule annotationAdd BLAST66

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 12Poly-Phe8

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063723
HOGENOMiHOG000029869
InParanoidiP33753
KOiK15331
OMAiGFWQVHR
OrthoDBiEOG092C156C

Family and domain databases

InterProiView protein in InterPro
IPR030390 MeTrfase_TrmA_AS
IPR030391 MeTrfase_TrmA_CS
IPR012340 NA-bd_OB-fold
IPR029063 SAM-dependent_MTases
IPR002792 TRAM_dom
IPR025795 tRNA_(uracil-5-)_MeTrfase
IPR010280 U5_MeTrfase_fam
PANTHERiPTHR11061 PTHR11061, 1 hit
PfamiView protein in Pfam
PF01938 TRAM, 1 hit
PF05958 tRNA_U5-meth_tr, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51687 SAM_MT_RNA_M5U, 1 hit
PS50926 TRAM, 1 hit
PS01230 TRMA_1, 1 hit
PS01231 TRMA_2, 1 hit

Sequencei

Sequence statusi: Complete.

P33753-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEQFEFSFF FFENSDNKVK YKAHLISSIK RWSIITCMRC FWTVQKSIFK
60 70 80 90 100
ARFFACRNFV KKHNYKLIST MTGSTEMVPP TMKHTVDNKR LSSPLTDSGN
110 120 130 140 150
RRTKKPKLRK YKAKKVETTS PMGVLEFEVN DLLKSQNLSR EQVLNDVTSI
160 170 180 190 200
LNDKSSTDGP IVLQYHREVK NVKVLEITSN GNGLALIDNP VETEKKQVVI
210 220 230 240 250
IPFGLPGDVV NIKVFKTHPY YVESDLLDVV EKSPMRRDDL IRDKYFGKSS
260 270 280 290 300
GSQLEFLTYD DQLELKRKTI MNAYKFFAPR LVAEKLLPPF DTTVASPLQF
310 320 330 340 350
GYRTKITPHF DMPKRKQKEL SVRPPLGFGQ KGRPQWRKDT LDIGGHGSIL
360 370 380 390 400
DIDECVLATE VLNKGLTNER RKFEQEFKNY KKGATILLRE NTTILDPSKP
410 420 430 440 450
TLEQLTEEAS RDENGDISYV EVEDKKNNVR LAKTCVTNPR QIVTEYVDGY
460 470 480 490 500
TFNFSAGEFF QNNNSILPIV TKYVRDNLQA PAKGDDNKTK FLVDAYCGSG
510 520 530 540 550
LFSICSSKGV DKVIGVEISA DSVSFAEKNA KANGVENCRF IVGKAEKLFE
560 570 580 590 600
SIDTPSENTS VILDPPRKGC DELFLKQLAA YNPAKIIYIS CNVHSQARDV
610 620 630
EYFLKETENG SAHQIESIRG FDFFPQTHHV ESVCIMKRI
Length:639
Mass (Da):72,852
Last modified:July 25, 2006 - v2
Checksum:iE7EC5B1378F7C361
GO

Sequence cautioni

The sequence CAA78500 differs from that shown. Cloning artifact which fused this protein with RHO4.Curated
The sequence CAA81021 differs from that shown. Reason: Frameshift at position 582.Curated
The sequence CAA82134 differs from that shown. Reason: Frameshift at position 582.Curated
The sequence CAC01942 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24H → Y in CAA78500 (PubMed:1408836).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25734 Genomic DNA Translation: CAA81021.1 Frameshift.
AJ250970 Genomic DNA Translation: CAC01942.1 Different initiation.
Z28281 Genomic DNA Translation: CAA82134.1 Frameshift.
Z14126 Genomic DNA Translation: CAA78500.1 Sequence problems.
AY260897 Genomic DNA Translation: AAP21765.1
BK006944 Genomic DNA Translation: DAA09207.1
PIRiS37744
RefSeqiNP_012982.2, NM_001179846.1

Genome annotation databases

EnsemblFungiiYKR056W; YKR056W; YKR056W
GeneIDi853930
KEGGisce:YKR056W

Similar proteinsi

Entry informationi

Entry nameiTRM2_YEAST
AccessioniPrimary (citable) accession number: P33753
Secondary accession number(s): D6VXB7
, P30618, Q86ZR6, Q9HGQ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 25, 2006
Last modified: May 23, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health