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P33752 (CTFA_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Butyrate--acetoacetate CoA-transferase subunit A

Short name=Coat A
EC=2.8.3.9
Alternative name(s):
Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit A
Gene names
Name:ctfA
Ordered Locus Names:CA_P0163
Encoded onPlasmid pSOL1
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length218 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation.

Catalytic activity

Butanoyl-CoA + acetoacetate = butanoate + acetoacetyl-CoA.

Subunit structure

Heterodimer.

Induction

Induced during switch to solvent production.

Sequence similarities

Belongs to the 3-oxoacid CoA-transferase subunit A family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 218218Butyrate--acetoacetate CoA-transferase subunit A
PRO_0000157914

Regions

Region24 – 307CoA-binding Potential

Experimental info

Sequence conflict1511V → I in AAB53234. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P33752 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 4E83A17413D19CE1

FASTA21823,641
        10         20         30         40         50         60 
MNSKIIRFEN LRSFFKDGMT IMIGGFLNCG TPTKLIDFLV NLNIKNLTII SNDTCYPNTG 

        70         80         90        100        110        120 
IGKLISNNQV KKLIASYIGS NPDTGKKLFN NELEVELSPQ GTLVERIRAG GSGLGGVLTK 

       130        140        150        160        170        180 
TGLGTLIEKG KKKISINGTE YLLELPLTAD VALIKGSIVD EAGNTFYKGT TKNFNPYMAM 

       190        200        210 
AAKTVIVEAE NLVSCEKLEK EKAMTPGVLI NYIVKEPA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis."
Fischer R.J., Helms J., Duerre P.
J. Bacteriol. 175:6959-6969(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]"Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824."
Petersen D.J., Cary J.W., Vanderleyden J., Bennett G.N.
Gene 123:93-97(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20.
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[3]Petersen D.J., Bennett G.N.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO C-TERMINUS.
[4]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[5]"Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824."
Nair R.V., Bennett G.N., Papoutsakis E.T.
J. Bacteriol. 176:871-885(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
[6]"Cloning and expression of Clostridium acetobutylicum ATCC 824 acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase in Escherichia coli."
Cary J.W., Petersen D.J., Papoutsakis E.T., Bennett G.N.
Appl. Environ. Microbiol. 56:1576-1583(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-14.
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X72831 Genomic DNA. Translation: CAA51345.1.
M93363 Genomic DNA. Translation: AAB53234.1.
AE001438 Genomic DNA. Translation: AAK76908.1.
L14817 Genomic DNA. No translation available.
PIRB49346.
RefSeqNP_149326.1. NC_001988.2.

3D structure databases

ProteinModelPortalP33752.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_P0163.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK76908; AAK76908; CA_P0163.
GeneID1116168.
KEGGcac:CA_P0163.
PATRIC32034212. VBICloAce74127_0164.

Phylogenomic databases

eggNOGCOG1788.
HOGENOMHOG000221244.
KOK01034.
OMANKAGRNF.
OrthoDBEOG6HMXJG.
ProtClustDBCLSK865492.

Enzyme and pathway databases

BioCycCACE272562:GJIH-4008-MONOMER.
MetaCyc:COATACLOS-MONOMER.
SABIO-RKP33752.

Family and domain databases

InterProIPR012792. 3-oxoacid_CoA-transf_A.
IPR004165. CoA_trans_fam_I.
IPR004163. CoA_transf_BS.
[Graphical view]
PANTHERPTHR13707. PTHR13707. 1 hit.
PfamPF01144. CoA_trans. 1 hit.
[Graphical view]
SMARTSM00882. CoA_trans. 1 hit.
[Graphical view]
TIGRFAMsTIGR02429. pcaI_scoA_fam. 1 hit.
PROSITEPS01273. COA_TRANSF_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCTFA_CLOAB
AccessionPrimary (citable) accession number: P33752
Secondary accession number(s): O06494
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families