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P33752

- CTFA_CLOAB

UniProt

P33752 - CTFA_CLOAB

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Protein

Butyrate--acetoacetate CoA-transferase subunit A

Gene

ctfA

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation.

Catalytic activityi

Butanoyl-CoA + acetoacetate = butanoate + acetoacetyl-CoA.

GO - Molecular functioni

  1. butyrate-acetoacetate CoA-transferase activity Source: UniProtKB-EC
  2. CoA-transferase activity Source: MENGO
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciCACE272562:GJIH-4008-MONOMER.
MetaCyc:COATACLOS-MONOMER.
SABIO-RKP33752.

Names & Taxonomyi

Protein namesi
Recommended name:
Butyrate--acetoacetate CoA-transferase subunit A (EC:2.8.3.9)
Short name:
Coat A
Alternative name(s):
Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit A
Gene namesi
Name:ctfA
Ordered Locus Names:CA_P0163
Encoded oniPlasmid pSOL10 Publication
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000814: Plasmid pSOL1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 218218Butyrate--acetoacetate CoA-transferase subunit APRO_0000157914Add
BLAST

Expressioni

Inductioni

Induced during switch to solvent production.

Interactioni

Subunit structurei

Heterodimer.

Protein-protein interaction databases

STRINGi272562.CA_P0163.

Structurei

3D structure databases

ProteinModelPortaliP33752.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 307CoA-bindingSequence Analysis

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1788.
HOGENOMiHOG000221244.
KOiK01034.
OMAiTLIASHI.
OrthoDBiEOG6HMXJG.

Family and domain databases

InterProiIPR012792. 3-oxoacid_CoA-transf_A.
IPR004165. CoA_trans_fam_I.
IPR004163. CoA_transf_BS.
[Graphical view]
PANTHERiPTHR13707. PTHR13707. 1 hit.
PfamiPF01144. CoA_trans. 1 hit.
[Graphical view]
SMARTiSM00882. CoA_trans. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02429. pcaI_scoA_fam. 1 hit.
PROSITEiPS01273. COA_TRANSF_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33752-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSKIIRFEN LRSFFKDGMT IMIGGFLNCG TPTKLIDFLV NLNIKNLTII
60 70 80 90 100
SNDTCYPNTG IGKLISNNQV KKLIASYIGS NPDTGKKLFN NELEVELSPQ
110 120 130 140 150
GTLVERIRAG GSGLGGVLTK TGLGTLIEKG KKKISINGTE YLLELPLTAD
160 170 180 190 200
VALIKGSIVD EAGNTFYKGT TKNFNPYMAM AAKTVIVEAE NLVSCEKLEK
210
EKAMTPGVLI NYIVKEPA
Length:218
Mass (Da):23,641
Last modified:February 1, 1994 - v1
Checksum:i4E83A17413D19CE1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511V → I in AAB53234. (PubMed:8423010)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72831 Genomic DNA. Translation: CAA51345.1.
M93363 Genomic DNA. Translation: AAB53234.1.
AE001438 Genomic DNA. Translation: AAK76908.1.
L14817 Genomic DNA. No translation available.
PIRiB49346.
RefSeqiNP_149326.1. NC_001988.2.
WP_010890847.1. NC_001988.2.

Genome annotation databases

EnsemblBacteriaiAAK76908; AAK76908; CA_P0163.
GeneIDi1116168.
KEGGicac:CA_P0163.
PATRICi32034212. VBICloAce74127_0164.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72831 Genomic DNA. Translation: CAA51345.1 .
M93363 Genomic DNA. Translation: AAB53234.1 .
AE001438 Genomic DNA. Translation: AAK76908.1 .
L14817 Genomic DNA. No translation available.
PIRi B49346.
RefSeqi NP_149326.1. NC_001988.2.
WP_010890847.1. NC_001988.2.

3D structure databases

ProteinModelPortali P33752.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272562.CA_P0163.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK76908 ; AAK76908 ; CA_P0163 .
GeneIDi 1116168.
KEGGi cac:CA_P0163.
PATRICi 32034212. VBICloAce74127_0164.

Phylogenomic databases

eggNOGi COG1788.
HOGENOMi HOG000221244.
KOi K01034.
OMAi TLIASHI.
OrthoDBi EOG6HMXJG.

Enzyme and pathway databases

BioCyci CACE272562:GJIH-4008-MONOMER.
MetaCyc:COATACLOS-MONOMER.
SABIO-RK P33752.

Family and domain databases

InterProi IPR012792. 3-oxoacid_CoA-transf_A.
IPR004165. CoA_trans_fam_I.
IPR004163. CoA_transf_BS.
[Graphical view ]
PANTHERi PTHR13707. PTHR13707. 1 hit.
Pfami PF01144. CoA_trans. 1 hit.
[Graphical view ]
SMARTi SM00882. CoA_trans. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02429. pcaI_scoA_fam. 1 hit.
PROSITEi PS01273. COA_TRANSF_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis."
    Fischer R.J., Helms J., Duerre P.
    J. Bacteriol. 175:6959-6969(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  2. "Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824."
    Petersen D.J., Cary J.W., Vanderleyden J., Bennett G.N.
    Gene 123:93-97(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20.
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  3. Petersen D.J., Bennett G.N.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  5. "Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824."
    Nair R.V., Bennett G.N., Papoutsakis E.T.
    J. Bacteriol. 176:871-885(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
  6. "Cloning and expression of Clostridium acetobutylicum ATCC 824 acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase in Escherichia coli."
    Cary J.W., Petersen D.J., Papoutsakis E.T., Bennett G.N.
    Appl. Environ. Microbiol. 56:1576-1583(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-14.
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiCTFA_CLOAB
AccessioniPrimary (citable) accession number: P33752
Secondary accession number(s): O06494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 1, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3