P33752 (CTFA_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Butyrate--acetoacetate CoA-transferase subunit A
Short name=Coat A
EC=2.8.3.9

Alternative name(s):
Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit A

Gene names

Name:	ctfA
Ordered Locus Names:	CA_P0163

Encoded on

Plasmid pSOL1

Organism

Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]

Taxonomic identifier

272562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium acetobutylicum

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation.
Catalytic activity	Butanoyl-CoA + acetoacetate = butanoate + acetoacetyl-CoA.
Subunit structure	Heterodimer.
Induction	Induced during switch to solvent production.
Sequence similarities	Belongs to the 3-oxoacid CoA-transferase subunit A family.

Ontologies

Keywords
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Plasmid Reference proteome
Gene Ontology (GO)
Molecular_function	butyrate-acetoacetate CoA-transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Butyrate--acetoacetate CoA-transferase subunit A		PRO_0000157914
Regions
	Region	24 – 30	7	CoA-binding Potential
Experimental info
	Sequence conflict	151	1	V → I in AAB53234. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P33752 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 4E83A17413D19CE1	FASTA	218	23,641
	10 20 30 40 50 60 MNSKIIRFEN LRSFFKDGMT IMIGGFLNCG TPTKLIDFLV NLNIKNLTII SNDTCYPNTG 70 80 90 100 110 120 IGKLISNNQV KKLIASYIGS NPDTGKKLFN NELEVELSPQ GTLVERIRAG GSGLGGVLTK 130 140 150 160 170 180 TGLGTLIEKG KKKISINGTE YLLELPLTAD VALIKGSIVD EAGNTFYKGT TKNFNPYMAM 190 200 210 AAKTVIVEAE NLVSCEKLEK EKAMTPGVLI NYIVKEPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis." Fischer R.J., Helms J., Duerre P. J. Bacteriol. 175:6959-6969(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824." Petersen D.J., Cary J.W., Vanderleyden J., Bennett G.N. Gene 123:93-97(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[3]	Petersen D.J., Bennett G.N. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO C-TERMINUS.
[4]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[5]	"Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824." Nair R.V., Bennett G.N., Papoutsakis E.T. J. Bacteriol. 176:871-885(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
[6]	"Cloning and expression of Clostridium acetobutylicum ATCC 824 acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase in Escherichia coli." Cary J.W., Petersen D.J., Papoutsakis E.T., Bennett G.N. Appl. Environ. Microbiol. 56:1576-1583(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-14. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72831 Genomic DNA. Translation: CAA51345.1. M93363 Genomic DNA. Translation: AAB53234.1. AE001438 Genomic DNA. Translation: AAK76908.1. L14817 Genomic DNA. No translation available.
PIR	B49346.
RefSeq	NP_149326.1. NC_001988.2.
3D structure databases
ProteinModelPortal	P33752.
ModBase	Search...
Protein-protein interaction databases
STRING	272562.CA_P0163.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK76908; AAK76908; CA_P0163.
GeneID	1116168.
KEGG	cac:CA_P0163.
PATRIC	32034212. VBICloAce74127_0164.
Phylogenomic databases
eggNOG	COG1788.
HOGENOM	HOG000221244.
KO	K01036.
OMA	VDTGVGP.
ProtClustDB	CLSK865492.
Enzyme and pathway databases
BioCyc	CACE272562:GJIH-4008-MONOMER. MetaCyc:COATACLOS-MONOMER.
SABIO-RK	P33752.
Family and domain databases
InterPro	IPR012792. 3-oxoacid_CoA-transf_A. IPR004165. CoA_trans_fam_I. IPR004163. CoA_transf_BS. [Graphical view]
PANTHER	PTHR13707. PTHR13707. 1 hit.
Pfam	PF01144. CoA_trans. 1 hit. [Graphical view]
SMART	SM00882. CoA_trans. 1 hit. [Graphical view]
TIGRFAMs	TIGR02429. pcaI_scoA_fam. 1 hit.
PROSITE	PS01273. COA_TRANSF_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTFA_CLOAB

Accession

Primary (citable) accession number: P33752
Secondary accession number(s): O06494

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program