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P33752

- CTFA_CLOAB

UniProt

P33752 - CTFA_CLOAB

Protein

Butyrate--acetoacetate CoA-transferase subunit A

Gene

ctfA

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation.

    Catalytic activityi

    Butanoyl-CoA + acetoacetate = butanoate + acetoacetyl-CoA.

    GO - Molecular functioni

    1. butyrate-acetoacetate CoA-transferase activity Source: UniProtKB-EC
    2. CoA-transferase activity Source: MENGO

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BioCyciCACE272562:GJIH-4008-MONOMER.
    MetaCyc:COATACLOS-MONOMER.
    SABIO-RKP33752.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Butyrate--acetoacetate CoA-transferase subunit A (EC:2.8.3.9)
    Short name:
    Coat A
    Alternative name(s):
    Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit A
    Gene namesi
    Name:ctfA
    Ordered Locus Names:CA_P0163
    Encoded oniPlasmid pSOL10 Publication
    OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
    Taxonomic identifieri272562 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000814: Plasmid pSOL1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 218218Butyrate--acetoacetate CoA-transferase subunit APRO_0000157914Add
    BLAST

    Expressioni

    Inductioni

    Induced during switch to solvent production.

    Interactioni

    Subunit structurei

    Heterodimer.

    Protein-protein interaction databases

    STRINGi272562.CA_P0163.

    Structurei

    3D structure databases

    ProteinModelPortaliP33752.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni24 – 307CoA-bindingSequence Analysis

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1788.
    HOGENOMiHOG000221244.
    KOiK01034.
    OMAiTLIASHI.
    OrthoDBiEOG6HMXJG.

    Family and domain databases

    InterProiIPR012792. 3-oxoacid_CoA-transf_A.
    IPR004165. CoA_trans_fam_I.
    IPR004163. CoA_transf_BS.
    [Graphical view]
    PANTHERiPTHR13707. PTHR13707. 1 hit.
    PfamiPF01144. CoA_trans. 1 hit.
    [Graphical view]
    SMARTiSM00882. CoA_trans. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02429. pcaI_scoA_fam. 1 hit.
    PROSITEiPS01273. COA_TRANSF_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33752-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSKIIRFEN LRSFFKDGMT IMIGGFLNCG TPTKLIDFLV NLNIKNLTII    50
    SNDTCYPNTG IGKLISNNQV KKLIASYIGS NPDTGKKLFN NELEVELSPQ 100
    GTLVERIRAG GSGLGGVLTK TGLGTLIEKG KKKISINGTE YLLELPLTAD 150
    VALIKGSIVD EAGNTFYKGT TKNFNPYMAM AAKTVIVEAE NLVSCEKLEK 200
    EKAMTPGVLI NYIVKEPA 218
    Length:218
    Mass (Da):23,641
    Last modified:February 1, 1994 - v1
    Checksum:i4E83A17413D19CE1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti151 – 1511V → I in AAB53234. (PubMed:8423010)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72831 Genomic DNA. Translation: CAA51345.1.
    M93363 Genomic DNA. Translation: AAB53234.1.
    AE001438 Genomic DNA. Translation: AAK76908.1.
    L14817 Genomic DNA. No translation available.
    PIRiB49346.
    RefSeqiNP_149326.1. NC_001988.2.
    WP_010890847.1. NC_001988.2.

    Genome annotation databases

    EnsemblBacteriaiAAK76908; AAK76908; CA_P0163.
    GeneIDi1116168.
    KEGGicac:CA_P0163.
    PATRICi32034212. VBICloAce74127_0164.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72831 Genomic DNA. Translation: CAA51345.1 .
    M93363 Genomic DNA. Translation: AAB53234.1 .
    AE001438 Genomic DNA. Translation: AAK76908.1 .
    L14817 Genomic DNA. No translation available.
    PIRi B49346.
    RefSeqi NP_149326.1. NC_001988.2.
    WP_010890847.1. NC_001988.2.

    3D structure databases

    ProteinModelPortali P33752.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272562.CA_P0163.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK76908 ; AAK76908 ; CA_P0163 .
    GeneIDi 1116168.
    KEGGi cac:CA_P0163.
    PATRICi 32034212. VBICloAce74127_0164.

    Phylogenomic databases

    eggNOGi COG1788.
    HOGENOMi HOG000221244.
    KOi K01034.
    OMAi TLIASHI.
    OrthoDBi EOG6HMXJG.

    Enzyme and pathway databases

    BioCyci CACE272562:GJIH-4008-MONOMER.
    MetaCyc:COATACLOS-MONOMER.
    SABIO-RK P33752.

    Family and domain databases

    InterProi IPR012792. 3-oxoacid_CoA-transf_A.
    IPR004165. CoA_trans_fam_I.
    IPR004163. CoA_transf_BS.
    [Graphical view ]
    PANTHERi PTHR13707. PTHR13707. 1 hit.
    Pfami PF01144. CoA_trans. 1 hit.
    [Graphical view ]
    SMARTi SM00882. CoA_trans. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02429. pcaI_scoA_fam. 1 hit.
    PROSITEi PS01273. COA_TRANSF_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis."
      Fischer R.J., Helms J., Duerre P.
      J. Bacteriol. 175:6959-6969(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
    2. "Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824."
      Petersen D.J., Cary J.W., Vanderleyden J., Bennett G.N.
      Gene 123:93-97(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20.
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
    3. Petersen D.J., Bennett G.N.
      Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
    5. "Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824."
      Nair R.V., Bennett G.N., Papoutsakis E.T.
      J. Bacteriol. 176:871-885(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
    6. "Cloning and expression of Clostridium acetobutylicum ATCC 824 acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase in Escherichia coli."
      Cary J.W., Petersen D.J., Papoutsakis E.T., Bennett G.N.
      Appl. Environ. Microbiol. 56:1576-1583(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-14.
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

    Entry informationi

    Entry nameiCTFA_CLOAB
    AccessioniPrimary (citable) accession number: P33752
    Secondary accession number(s): O06494
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3