P33751 (PAD1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylacrylic acid decarboxylase
Short name=PAD
EC=4.1.1.-

Gene names

Name:	PAD1
Synonyms:	POF1
Ordered Locus Names:	YDR538W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Confers resistance to cinnamic acid.
Cofactor	Binds 1 FMN per subunit By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 1750 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the polyprenyl p-hydroxybenzoate / phenylacrylic acid decarboxylases family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FMN
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cinnamic acid catabolic process Inferred from mutant phenotype PubMed 20471595Ref.1. Source: SGD
Cellular_component	mitochondrion Inferred from direct assay PubMed 14562095PubMed 17054397. Source: SGD
Molecular_function	carboxy-lyase activity Inferred from mutant phenotype PubMed 11693915Ref.1. Source: SGD mRNA binding Inferred from direct assay PubMed 20844764. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Phenylacrylic acid decarboxylase		PRO_0000134984
Regions
	Nucleotide binding	63 – 65	3	FMN By similarity
	Nucleotide binding	140 – 143	4	FMN By similarity
Sites
	Binding site	89	1	FMN By similarity
	Binding site	175	1	FMN By similarity
Experimental info
	Sequence conflict	76	1	V → L in AAA20484. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P33751 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 9EC09F1197D5C97D	FASTA	242	26,733
	10 20 30 40 50 60 MLLFPRRTNI AFFKTTGIFA NFPLLGRTIT TSPSFLTHKL SKEVTRASTS PPRPKRIVVA 70 80 90 100 110 120 ITGATGVALG IRLLQVLKEL SVETHLVISK WGAATMKYET DWEPHDVAAL ATKTYSVRDV 130 140 150 160 170 180 SACISSGSFQ HDGMIVVPCS MKSLAAIRIG FTEDLITRAA DVSIKENRKL LLVTRETPLS 190 200 210 220 230 240 SIHLENMLSL CRAGVIIFPP VPAFYTRPKS LHDLLEQSVG RILDCFGIHA DTFPRWEGIK SK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PAD1 encodes phenylacrylic acid decarboxylase which confers resistance to cinnamic acid in Saccharomyces cerevisiae." Clausen M., Lamb C.J., Megnet R., Doerner P.W. Gene 142:107-112(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L09263 Genomic DNA. Translation: AAA20484.1. U43834 Genomic DNA. Translation: AAB64980.1. BK006938 Genomic DNA. Translation: DAA12367.1.
PIR	S62017.
RefSeq	NP_010827.3. NM_001180846.3.
3D structure databases
ProteinModelPortal	P33751.
SMR	P33751. Positions 56-228.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5454N.
IntAct	P33751. 2 interactions.
MINT	MINT-503534.
STRING	4932.YDR538W.
Proteomic databases
PaxDb	P33751.
PeptideAtlas	P33751.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR538W; YDR538W; YDR538W.
GeneID	852150.
KEGG	sce:YDR538W. sce:YDR542W.
Organism-specific databases
CYGD	YDR538w.
SGD	S000002946. PAD1.
Phylogenomic databases
eggNOG	COG0163.
HOGENOM	HOG000225437.
KO	K12468.
OMA	TPWAGIR.
OrthoDB	EOG41K2NH.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-623.
Gene expression databases
ArrayExpress	P33751.
Genevestigator	P33751.
GermOnline	YDR538W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.1950. 1 hit.
InterPro	IPR003382. Flavoprotein. IPR004507. PAD1. [Graphical view]
Pfam	PF02441. Flavoprotein. 1 hit. [Graphical view]
SUPFAM	SSF52507. Flavoprotein. 1 hit.
TIGRFAMs	TIGR00421. ubiX_pad. 1 hit.
ProtoNet	Search...
Other
NextBio	970576.

Entry information

Entry name

PAD1_YEAST

Accession

Primary (citable) accession number: P33751
Secondary accession number(s): D6VTF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program