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Protein

Zinc finger protein MSN2

Gene

MSN2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Positive transcriptional factor that acts as a component of the stress responsive system. Recognizes and binds to the stress response element (STRE) which is involved in the response to various forms of stress (heat, oxidative, osmotic, etc.). Involved in the regulation of the CTT1, DDR2, HSP12 genes. May be regulated via WHI2-PSR1 complex phosphatase activity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri647 – 66519C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri676 – 69823C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • age-dependent response to oxidative stress involved in chronological cell aging Source: SGD
  • cellular response to blue light Source: SGD
  • cellular response to methylmercury Source: SGD
  • chromatin remodeling Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to acidic pH Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to a hypotonic environment Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to alkaline pH Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to cold Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to ethanol Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to freezing Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to glucose starvation Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to heat stress Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to hydrogen peroxide Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to hydrostatic pressure Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to increased salt Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to nitrosative stress Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to zinc ion starvation Source: SGD
  • regulation of replicative cell aging by regulation of transcription from RNA polymerase II promoter in response to caloric restriction Source: SGD
  • replicative cell aging Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32742-MONOMER.
ReactomeiR-SCE-3214841. PKMTs methylate histone lysines.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein MSN2
Alternative name(s):
Multicopy suppressor of SNF1 protein 2
Gene namesi
Name:MSN2
Ordered Locus Names:YMR037C
ORF Names:YM9532.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR037C.
SGDiS000004640. MSN2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 704704Zinc finger protein MSN2PRO_0000046810Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei288 – 2881PhosphoserineCombined sources
Modified residuei304 – 3041PhosphoserineCombined sources
Modified residuei451 – 4511PhosphoserineCombined sources
Modified residuei582 – 5821PhosphoserineCombined sources
Modified residuei633 – 6331PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33748.
PeptideAtlasiP33748.

PTM databases

iPTMnetiP33748.

Interactioni

Subunit structurei

Interacts with WHI2.1 Publication

Protein-protein interaction databases

BioGridi35209. 124 interactions.
DIPiDIP-8185N.
IntActiP33748. 63 interactions.
MINTiMINT-1758175.

Structurei

3D structure databases

ProteinModelPortaliP33748.
SMRiP33748. Positions 613-694.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 109109Asp-rich (acidic)Add
BLAST
Compositional biasi260 – 27920Asp-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri647 – 66519C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri676 – 69823C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000063676.
InParanoidiP33748.
KOiK09468.
OrthoDBiEOG7K0ZMS.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVDHDFNSE DILFPIESMS SIQYVENNNP NNINNDVIPY SLDIKNTVLD
60 70 80 90 100
SADLNDIQNQ ETSLNLGLPP LSFDSPLPVT ETIPSTTDNS LHLKADSNKN
110 120 130 140 150
RDARTIENDS EIKSTNNASG SGANQYTTLT SPYPMNDILY NMNNPLQSPS
160 170 180 190 200
PSSVPQNPTI NPPINTASNE TNLSPQTSNG NETLISPRAQ QHTSIKDNRL
210 220 230 240 250
SLPNGANSNL FIDTNPNNLN EKLRNQLNSD TNSYSNSISN SNSNSTGNLN
260 270 280 290 300
SSYFNSLNID SMLDDYVSSD LLLNDDDDDT NLSRRRFSDV ITNQFPSMTN
310 320 330 340 350
SRNSISHSLD LWNHPKINPS NRNTNLNITT NSTSSSNASP NTTTMNANAD
360 370 380 390 400
SNIAGNPKNN DATIDNELTQ ILNEYNMNFN DNLGTSTSGK NKSACPSSFD
410 420 430 440 450
ANAMTKINPS QQLQQQLNRV QHKQLTSSHN NSSTNMKSFN SDLYSRRQRA
460 470 480 490 500
SLPIIDDSLS YDLVNKQDED PKNDMLPNSN LSSSQQFIKP SMILSDNASV
510 520 530 540 550
IAKVATTGLS NDMPFLTEEG EQNANSTPNF DLSITQMNMA PLSPASSSST
560 570 580 590 600
SLATNHFYHH FPQQGHHTMN SKIGSSLRRR KSAVPLMGTV PLTNQQNNIS
610 620 630 640 650
SSSVNSTGNG AGVTKERRPS YRRKSMTPSR RSSVVIESTK ELEEKPFHCH
660 670 680 690 700
ICPKSFKRSE HLKRHVRSVH SNERPFACHI CDKKFSRSDN LSQHIKTHKK

HGDI
Length:704
Mass (Da):77,861
Last modified:February 1, 1994 - v1
Checksum:iEDF6F07446819DF1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08838 Genomic DNA. Translation: AAA34806.1.
Z48502 Genomic DNA. Translation: CAA88403.1.
BK006946 Genomic DNA. Translation: DAA09936.1.
PIRiS39004.
RefSeqiNP_013751.1. NM_001182534.1.

Genome annotation databases

EnsemblFungiiYMR037C; YMR037C; YMR037C.
GeneIDi855053.
KEGGisce:YMR037C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08838 Genomic DNA. Translation: AAA34806.1.
Z48502 Genomic DNA. Translation: CAA88403.1.
BK006946 Genomic DNA. Translation: DAA09936.1.
PIRiS39004.
RefSeqiNP_013751.1. NM_001182534.1.

3D structure databases

ProteinModelPortaliP33748.
SMRiP33748. Positions 613-694.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35209. 124 interactions.
DIPiDIP-8185N.
IntActiP33748. 63 interactions.
MINTiMINT-1758175.

PTM databases

iPTMnetiP33748.

Proteomic databases

MaxQBiP33748.
PeptideAtlasiP33748.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR037C; YMR037C; YMR037C.
GeneIDi855053.
KEGGisce:YMR037C.

Organism-specific databases

EuPathDBiFungiDB:YMR037C.
SGDiS000004640. MSN2.

Phylogenomic databases

GeneTreeiENSGT00530000063676.
InParanoidiP33748.
KOiK09468.
OrthoDBiEOG7K0ZMS.

Enzyme and pathway databases

BioCyciYEAST:G3O-32742-MONOMER.
ReactomeiR-SCE-3214841. PKMTs methylate histone lysines.

Miscellaneous databases

PROiP33748.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two homologous zinc finger genes identified by multicopy suppression in a SNF1 protein kinase mutant of Saccharomyces cerevisiae."
    Estruch F., Carlson M.
    Mol. Cell. Biol. 13:3872-3881(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE)."
    Martinez-Pastor M.T., Marchler G., Schueller C., Marchler-Bauer A., Ruis H., Estruch F.
    EMBO J. 15:2227-2235(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Yeast Whi2 and Psr1-phosphatase form a complex and regulate STRE-mediated gene expression."
    Kaida D., Yashiroda H., Toh-e A., Kikuchi Y.
    Genes Cells 7:543-552(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WHI2, PHOSPHORYLATION.
  6. "Oscillatory nucleocytoplasmic shuttling of the general stress response transcriptional activators Msn2 and Msn4 in Saccharomyces cerevisiae."
    Jacquet M., Renault G., Lallet S., De Mey J., Goldbeter A.
    J. Cell Biol. 161:497-505(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOCYTOPLASMIC SHUTTLING.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304 AND SER-633, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288 AND SER-451, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288; SER-304; SER-451; SER-582 AND SER-633, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMSN2_YEAST
AccessioniPrimary (citable) accession number: P33748
Secondary accession number(s): D6VZL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 8, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 125 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.