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Protein

Aldehyde-alcohol dehydrogenase

Gene

adhE

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has both aldehyde and alcohol dehydrogenase activities. Can use acetaldehyde, butyraldehyde, butanol and ethanol.1 Publication

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.1 Publication
An aldehyde + NAD+ + H2O = a carboxylate + NADH.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei244By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi420 – 425NADSequence analysis6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:AADCLOS-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde-alcohol dehydrogenase1 Publication
Short name:
AAD1 Publication
Including the following 2 domains:
Alcohol dehydrogenase1 Publication (EC:1.1.1.11 Publication)
Short name:
ADH1 Publication
Aldehyde dehydrogenase1 Publication (EC:1.2.1.31 Publication)
Short name:
ALDH1 Publication
Gene namesi
Name:adhE1 Publication
Synonyms:aad1 Publication
Ordered Locus Names:CA_P0162
Encoded oniPlasmid pSOL10 Publication
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000814 Componenti: Plasmid pSOL1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878391 – 862Aldehyde-alcohol dehydrogenaseAdd BLAST862

Expressioni

Inductioni

Induced during switch to solvent production.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP33744.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the aldehyde dehydrogenase family.Curated
In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.Curated

Phylogenomic databases

HOGENOMiHOG000025256.
KOiK04072.
OMAiVELNEPF.

Family and domain databases

Gene3Di3.40.309.10. 2 hits.
3.40.605.10. 1 hit.
InterProiIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012079. Bifunc_Ald-ADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
PF00465. Fe-ADH. 1 hit.
[Graphical view]
PIRSFiPIRSF000111. ALDH_ADH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00913. ADH_IRON_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVTTVKELD EKLKVIKEAQ KKFSCYSQEM VDEIFRNAAM AAIDARIELA
60 70 80 90 100
KAAVLETGMG LVEDKVIKNH FAGEYIYNKY KDEKTCGIIE RNEPYGITKI
110 120 130 140 150
AEPIGVVAAI IPVTNPTSTT IFKSLISLKT RNGIFFSPHP RAKKSTILAA
160 170 180 190 200
KTILDAAVKS GAPENIIGWI DEPSIELTQY LMQKADITLA TGGPSLVKSA
210 220 230 240 250
YSSGKPAIGV GPGNTPVIID ESAHIKMAVS SIILSKTYDN GVICASEQSV
260 270 280 290 300
IVLKSIYNKV KDEFQERGAY IIKKNELDKV REVIFKDGSV NPKIVGQSAY
310 320 330 340 350
TIAAMAGIKV PKTTRILIGE VTSLGEEEPF AHEKLSPVLA MYEADNFDDA
360 370 380 390 400
LKKAVTLINL GGLGHTSGIY ADEIKARDKI DRFSSAMKTV RTFVNIPTSQ
410 420 430 440 450
GASGDLYNFR IPPSFTLGCG FWGGNSVSEN VGPKHLLNIK TVAERRENML
460 470 480 490 500
WFRVPHKVYF KFGCLQFALK DLKDLKKKRA FIVTDSDPYN LNYVDSIIKI
510 520 530 540 550
LEHLDIDFKV FNKVGREADL KTIKKATEEM SSFMPDTIIA LGGTPEMSSA
560 570 580 590 600
KLMWVLYEHP EVKFEDLAIK FMDIRKRIYT FPKLGKKAML VAITTSAGSG
610 620 630 640 650
SEVTPFALVT DNNTGNKYML ADYEMTPNMA IVDAELMMKM PKGLTAYSGI
660 670 680 690 700
DALVNSIEAY TSVYASEYTN GLALEAIRLI FKYLPEAYKN GRTNEKAREK
710 720 730 740 750
MAHASTMAGM ASANAFLGLC HSMAIKLSSE HNIPSGIANA LLIEEVIKFN
760 770 780 790 800
AVDNPVKQAP CPQYKYPNTI FRYARIADYI KLGGNTDEEK VDLLINKIHE
810 820 830 840 850
LKKALNIPTS IKDAGVLEEN FYSSLDRISE LALDDQCTGA NPRFPLTSEI
860
KEMYINCFKK QP
Length:862
Mass (Da):95,321
Last modified:August 29, 2001 - v3
Checksum:iBE09E32B28DD08B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72831 Genomic DNA. Translation: CAA51344.1.
L14817 Genomic DNA. Translation: AAD04638.1.
AE001438 Genomic DNA. Translation: AAK76907.1.
PIRiA49346.
RefSeqiNP_149325.1. NC_001988.2.
WP_010890846.1. NC_001988.2.

Genome annotation databases

EnsemblBacteriaiAAK76907; AAK76907; CA_P0162.
GeneIDi1116167.
KEGGicac:CA_P0162.
PATRICi32034210. VBICloAce74127_0163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72831 Genomic DNA. Translation: CAA51344.1.
L14817 Genomic DNA. Translation: AAD04638.1.
AE001438 Genomic DNA. Translation: AAK76907.1.
PIRiA49346.
RefSeqiNP_149325.1. NC_001988.2.
WP_010890846.1. NC_001988.2.

3D structure databases

ProteinModelPortaliP33744.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK76907; AAK76907; CA_P0162.
GeneIDi1116167.
KEGGicac:CA_P0162.
PATRICi32034210. VBICloAce74127_0163.

Phylogenomic databases

HOGENOMiHOG000025256.
KOiK04072.
OMAiVELNEPF.

Enzyme and pathway databases

BioCyciMetaCyc:AADCLOS-MONOMER.

Family and domain databases

Gene3Di3.40.309.10. 2 hits.
3.40.605.10. 1 hit.
InterProiIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012079. Bifunc_Ald-ADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
PF00465. Fe-ADH. 1 hit.
[Graphical view]
PIRSFiPIRSF000111. ALDH_ADH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00913. ADH_IRON_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADHE_CLOAB
AccessioniPrimary (citable) accession number: P33744
Secondary accession number(s): Q45808, Q45809
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 29, 2001
Last modified: November 2, 2016
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.