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P33744 (ADHE_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aldehyde-alcohol dehydrogenase

Including the following 2 domains:

  1. Alcohol dehydrogenase
    Short name=ADH
    EC=1.1.1.1
  2. Acetaldehyde dehydrogenase [acetylating]
    Short name=ACDH
    EC=1.2.1.10
Gene names
Name:adhE
Synonyms:aad
Ordered Locus Names:CA_P0162
Encoded onPlasmid pSOL1
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length862 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This enzyme has probably two activities: ADH, and ACDH.

Catalytic activity

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH.

Induction

Induced during switch to solvent production.

Sequence similarities

In the N-terminal section; belongs to the aldehyde dehydrogenase family.

In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 862862Aldehyde-alcohol dehydrogenase
PRO_0000087839

Regions

Nucleotide binding420 – 4256NAD Potential

Sites

Active site2441 By similarity

Sequences

Sequence LengthMass (Da)Tools
P33744 [UniParc].

Last modified August 29, 2001. Version 3.
Checksum: BE09E32B28DD08B0

FASTA86295,321
        10         20         30         40         50         60 
MKVTTVKELD EKLKVIKEAQ KKFSCYSQEM VDEIFRNAAM AAIDARIELA KAAVLETGMG 

        70         80         90        100        110        120 
LVEDKVIKNH FAGEYIYNKY KDEKTCGIIE RNEPYGITKI AEPIGVVAAI IPVTNPTSTT 

       130        140        150        160        170        180 
IFKSLISLKT RNGIFFSPHP RAKKSTILAA KTILDAAVKS GAPENIIGWI DEPSIELTQY 

       190        200        210        220        230        240 
LMQKADITLA TGGPSLVKSA YSSGKPAIGV GPGNTPVIID ESAHIKMAVS SIILSKTYDN 

       250        260        270        280        290        300 
GVICASEQSV IVLKSIYNKV KDEFQERGAY IIKKNELDKV REVIFKDGSV NPKIVGQSAY 

       310        320        330        340        350        360 
TIAAMAGIKV PKTTRILIGE VTSLGEEEPF AHEKLSPVLA MYEADNFDDA LKKAVTLINL 

       370        380        390        400        410        420 
GGLGHTSGIY ADEIKARDKI DRFSSAMKTV RTFVNIPTSQ GASGDLYNFR IPPSFTLGCG 

       430        440        450        460        470        480 
FWGGNSVSEN VGPKHLLNIK TVAERRENML WFRVPHKVYF KFGCLQFALK DLKDLKKKRA 

       490        500        510        520        530        540 
FIVTDSDPYN LNYVDSIIKI LEHLDIDFKV FNKVGREADL KTIKKATEEM SSFMPDTIIA 

       550        560        570        580        590        600 
LGGTPEMSSA KLMWVLYEHP EVKFEDLAIK FMDIRKRIYT FPKLGKKAML VAITTSAGSG 

       610        620        630        640        650        660 
SEVTPFALVT DNNTGNKYML ADYEMTPNMA IVDAELMMKM PKGLTAYSGI DALVNSIEAY 

       670        680        690        700        710        720 
TSVYASEYTN GLALEAIRLI FKYLPEAYKN GRTNEKAREK MAHASTMAGM ASANAFLGLC 

       730        740        750        760        770        780 
HSMAIKLSSE HNIPSGIANA LLIEEVIKFN AVDNPVKQAP CPQYKYPNTI FRYARIADYI 

       790        800        810        820        830        840 
KLGGNTDEEK VDLLINKIHE LKKALNIPTS IKDAGVLEEN FYSSLDRISE LALDDQCTGA 

       850        860 
NPRFPLTSEI KEMYINCFKK QP 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis."
Fischer R.J., Helms J., Duerre P.
J. Bacteriol. 175:6959-6969(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]"Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824."
Nair R.V., Bennett G.N., Papoutsakis E.T.
J. Bacteriol. 176:871-885(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[3]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X72831 Genomic DNA. Translation: CAA51344.1.
L14817 Genomic DNA. Translation: AAD04638.1.
AE001438 Genomic DNA. Translation: AAK76907.1.
PIRA49346.
RefSeqNP_149325.1. NC_001988.2.

3D structure databases

ProteinModelPortalP33744.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_P0162.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK76907; AAK76907; CA_P0162.
GeneID1116167.
KEGGcac:CA_P0162.
PATRIC32034210. VBICloAce74127_0163.

Phylogenomic databases

eggNOGCOG1012.
HOGENOMHOG000025256.
KOK04072.
OMANAVEQAC.
OrthoDBEOG6TFCQS.
ProtClustDBPRK13805.

Enzyme and pathway databases

BioCycCACE272562:GJIH-4007-MONOMER.
MetaCyc:AADCLOS-MONOMER.

Family and domain databases

Gene3D3.40.309.10. 2 hits.
3.40.605.10. 1 hit.
InterProIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012079. Bifunc_Ald/ADH.
[Graphical view]
PfamPF00171. Aldedh. 1 hit.
PF00465. Fe-ADH. 1 hit.
[Graphical view]
PIRSFPIRSF000111. ALDH_ADH. 1 hit.
SUPFAMSSF53720. SSF53720. 1 hit.
PROSITEPS00913. ADH_IRON_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameADHE_CLOAB
AccessionPrimary (citable) accession number: P33744
Secondary accession number(s): Q45808, Q45809
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 29, 2001
Last modified: February 19, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families