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Protein

Aldehyde-alcohol dehydrogenase

Gene

adhE

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme has probably two activities: ADH, and ACDH.

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.
Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei244 – 2441By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi420 – 4256NADSequence Analysis

GO - Molecular functioni

  1. acetaldehyde dehydrogenase (acetylating) activity Source: UniProtKB-EC
  2. alcohol dehydrogenase (NAD) activity Source: CACAO
  3. metal ion binding Source: InterPro

GO - Biological processi

  1. alcohol metabolic process Source: InterPro
  2. carbon utilization Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciCACE272562:GJIH-4007-MONOMER.
MetaCyc:AADCLOS-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde-alcohol dehydrogenase
Including the following 2 domains:
Alcohol dehydrogenase (EC:1.1.1.1)
Short name:
ADH
Acetaldehyde dehydrogenase [acetylating] (EC:1.2.1.10)
Short name:
ACDH
Gene namesi
Name:adhE
Synonyms:aad
Ordered Locus Names:CA_P0162
Encoded oniPlasmid pSOL10 Publication
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000814 Componenti: Plasmid pSOL1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 862862Aldehyde-alcohol dehydrogenasePRO_0000087839Add
BLAST

Expressioni

Inductioni

Induced during switch to solvent production.

Interactioni

Protein-protein interaction databases

STRINGi272562.CA_P0162.

Structurei

3D structure databases

ProteinModelPortaliP33744.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the aldehyde dehydrogenase family.Curated
In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000025256.
KOiK04072.
OMAiVELNEPF.
OrthoDBiEOG6TFCQS.

Family and domain databases

Gene3Di3.40.309.10. 2 hits.
3.40.605.10. 1 hit.
InterProiIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012079. Bifunc_Ald-ADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
PF00465. Fe-ADH. 1 hit.
[Graphical view]
PIRSFiPIRSF000111. ALDH_ADH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00913. ADH_IRON_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVTTVKELD EKLKVIKEAQ KKFSCYSQEM VDEIFRNAAM AAIDARIELA
60 70 80 90 100
KAAVLETGMG LVEDKVIKNH FAGEYIYNKY KDEKTCGIIE RNEPYGITKI
110 120 130 140 150
AEPIGVVAAI IPVTNPTSTT IFKSLISLKT RNGIFFSPHP RAKKSTILAA
160 170 180 190 200
KTILDAAVKS GAPENIIGWI DEPSIELTQY LMQKADITLA TGGPSLVKSA
210 220 230 240 250
YSSGKPAIGV GPGNTPVIID ESAHIKMAVS SIILSKTYDN GVICASEQSV
260 270 280 290 300
IVLKSIYNKV KDEFQERGAY IIKKNELDKV REVIFKDGSV NPKIVGQSAY
310 320 330 340 350
TIAAMAGIKV PKTTRILIGE VTSLGEEEPF AHEKLSPVLA MYEADNFDDA
360 370 380 390 400
LKKAVTLINL GGLGHTSGIY ADEIKARDKI DRFSSAMKTV RTFVNIPTSQ
410 420 430 440 450
GASGDLYNFR IPPSFTLGCG FWGGNSVSEN VGPKHLLNIK TVAERRENML
460 470 480 490 500
WFRVPHKVYF KFGCLQFALK DLKDLKKKRA FIVTDSDPYN LNYVDSIIKI
510 520 530 540 550
LEHLDIDFKV FNKVGREADL KTIKKATEEM SSFMPDTIIA LGGTPEMSSA
560 570 580 590 600
KLMWVLYEHP EVKFEDLAIK FMDIRKRIYT FPKLGKKAML VAITTSAGSG
610 620 630 640 650
SEVTPFALVT DNNTGNKYML ADYEMTPNMA IVDAELMMKM PKGLTAYSGI
660 670 680 690 700
DALVNSIEAY TSVYASEYTN GLALEAIRLI FKYLPEAYKN GRTNEKAREK
710 720 730 740 750
MAHASTMAGM ASANAFLGLC HSMAIKLSSE HNIPSGIANA LLIEEVIKFN
760 770 780 790 800
AVDNPVKQAP CPQYKYPNTI FRYARIADYI KLGGNTDEEK VDLLINKIHE
810 820 830 840 850
LKKALNIPTS IKDAGVLEEN FYSSLDRISE LALDDQCTGA NPRFPLTSEI
860
KEMYINCFKK QP
Length:862
Mass (Da):95,321
Last modified:August 29, 2001 - v3
Checksum:iBE09E32B28DD08B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72831 Genomic DNA. Translation: CAA51344.1.
L14817 Genomic DNA. Translation: AAD04638.1.
AE001438 Genomic DNA. Translation: AAK76907.1.
PIRiA49346.
RefSeqiNP_149325.1. NC_001988.2.
WP_010890846.1. NC_001988.2.

Genome annotation databases

EnsemblBacteriaiAAK76907; AAK76907; CA_P0162.
GeneIDi1116167.
KEGGicac:CA_P0162.
PATRICi32034210. VBICloAce74127_0163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72831 Genomic DNA. Translation: CAA51344.1.
L14817 Genomic DNA. Translation: AAD04638.1.
AE001438 Genomic DNA. Translation: AAK76907.1.
PIRiA49346.
RefSeqiNP_149325.1. NC_001988.2.
WP_010890846.1. NC_001988.2.

3D structure databases

ProteinModelPortaliP33744.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272562.CA_P0162.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK76907; AAK76907; CA_P0162.
GeneIDi1116167.
KEGGicac:CA_P0162.
PATRICi32034210. VBICloAce74127_0163.

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000025256.
KOiK04072.
OMAiVELNEPF.
OrthoDBiEOG6TFCQS.

Enzyme and pathway databases

BioCyciCACE272562:GJIH-4007-MONOMER.
MetaCyc:AADCLOS-MONOMER.

Family and domain databases

Gene3Di3.40.309.10. 2 hits.
3.40.605.10. 1 hit.
InterProiIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR012079. Bifunc_Ald-ADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
PF00465. Fe-ADH. 1 hit.
[Graphical view]
PIRSFiPIRSF000111. ALDH_ADH. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00913. ADH_IRON_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis."
    Fischer R.J., Helms J., Duerre P.
    J. Bacteriol. 175:6959-6969(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  2. "Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824."
    Nair R.V., Bennett G.N., Papoutsakis E.T.
    J. Bacteriol. 176:871-885(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiADHE_CLOAB
AccessioniPrimary (citable) accession number: P33744
Secondary accession number(s): Q45808, Q45809
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 29, 2001
Last modified: January 7, 2015
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.