P33744 (ADHE_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aldehyde-alcohol dehydrogenase

Including the following 2 domains:

1. Alcohol dehydrogenase
   Short name=ADH
   EC=1.1.1.1
2. Acetaldehyde dehydrogenase [acetylating]
   Short name=ACDH
   EC=1.2.1.10

Gene names

Name:	adhE
Synonyms:	aad
Ordered Locus Names:	CA_P0162

Encoded on

Plasmid pSOL1

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	862 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This enzyme has probably two activities: ADH, and ACDH.
Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH. Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH.
Induction	Induced during switch to solvent production.
Sequence similarities	In the N-terminal section; belongs to the aldehyde dehydrogenase family. In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme Plasmid Reference proteome
Gene Ontology (GO)
Biological process	alcohol metabolic process Inferred from electronic annotation. Source: InterPro carbon utilization Inferred from electronic annotation. Source: InterPro
Molecular function	acetaldehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: EC alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 862	862	Aldehyde-alcohol dehydrogenase		PRO_0000087839
Regions
	Nucleotide binding	420 – 425	6	NAD Potential
Sites
	Active site	244	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33744 [UniParc]. Last modified August 29, 2001. Version 3. Checksum: BE09E32B28DD08B0	FASTA	862	95,321
	10 20 30 40 50 60 MKVTTVKELD EKLKVIKEAQ KKFSCYSQEM VDEIFRNAAM AAIDARIELA KAAVLETGMG 70 80 90 100 110 120 LVEDKVIKNH FAGEYIYNKY KDEKTCGIIE RNEPYGITKI AEPIGVVAAI IPVTNPTSTT 130 140 150 160 170 180 IFKSLISLKT RNGIFFSPHP RAKKSTILAA KTILDAAVKS GAPENIIGWI DEPSIELTQY 190 200 210 220 230 240 LMQKADITLA TGGPSLVKSA YSSGKPAIGV GPGNTPVIID ESAHIKMAVS SIILSKTYDN 250 260 270 280 290 300 GVICASEQSV IVLKSIYNKV KDEFQERGAY IIKKNELDKV REVIFKDGSV NPKIVGQSAY 310 320 330 340 350 360 TIAAMAGIKV PKTTRILIGE VTSLGEEEPF AHEKLSPVLA MYEADNFDDA LKKAVTLINL 370 380 390 400 410 420 GGLGHTSGIY ADEIKARDKI DRFSSAMKTV RTFVNIPTSQ GASGDLYNFR IPPSFTLGCG 430 440 450 460 470 480 FWGGNSVSEN VGPKHLLNIK TVAERRENML WFRVPHKVYF KFGCLQFALK DLKDLKKKRA 490 500 510 520 530 540 FIVTDSDPYN LNYVDSIIKI LEHLDIDFKV FNKVGREADL KTIKKATEEM SSFMPDTIIA 550 560 570 580 590 600 LGGTPEMSSA KLMWVLYEHP EVKFEDLAIK FMDIRKRIYT FPKLGKKAML VAITTSAGSG 610 620 630 640 650 660 SEVTPFALVT DNNTGNKYML ADYEMTPNMA IVDAELMMKM PKGLTAYSGI DALVNSIEAY 670 680 690 700 710 720 TSVYASEYTN GLALEAIRLI FKYLPEAYKN GRTNEKAREK MAHASTMAGM ASANAFLGLC 730 740 750 760 770 780 HSMAIKLSSE HNIPSGIANA LLIEEVIKFN AVDNPVKQAP CPQYKYPNTI FRYARIADYI 790 800 810 820 830 840 KLGGNTDEEK VDLLINKIHE LKKALNIPTS IKDAGVLEEN FYSSLDRISE LALDDQCTGA 850 860 NPRFPLTSEI KEMYINCFKK QP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis." Fischer R.J., Helms J., Duerre P. J. Bacteriol. 175:6959-6969(1993) [PubMed: 8226639] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824." Nair R.V., Bennett G.N., Papoutsakis E.T. J. Bacteriol. 176:871-885(1994) [PubMed: 8300540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[3]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72831 Genomic DNA. Translation: CAA51344.1. L14817 Genomic DNA. Translation: AAD04638.1. AE001438 Genomic DNA. Translation: AAK76907.1.
PIR	A49346.
RefSeq	NP_149325.1. NC_001988.2.
3D structure databases
ProteinModelPortal	P33744.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1116167.
GenomeReviews	Gene locus CA_P0162 in contig AE001438_GR.
KEGG	cac:CA_P0162.
NMPDR	fig|272562.1.peg.160.
PATRIC	32034210. VBICloAce74127_0163.
Phylogenomic databases
HOGENOM	HBG285847.
PhylomeDB	P33744.
ProtClustDB	PRK13805.
Enzyme and pathway databases
BioCyc	CACE272562:CAP0162-MONOMER. MetaCyc:AADCLOS-MONOMER.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR012079. Bifunc_Ald/ADH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 2 hits. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
KO	K04072.
Pfam	PF00171. Aldedh. 1 hit. PF00465. Fe-ADH. 1 hit. [Graphical view]
PIRSF	PIRSF000111. ALDH_ADH. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADHE_CLOAB

Accession

Primary (citable) accession number: P33744
Secondary accession number(s): Q45808, Q45809

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	August 29, 2001
Last modified:	January 25, 2012
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program