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P33744

- ADHE_CLOAB

UniProt

P33744 - ADHE_CLOAB

Protein

Aldehyde-alcohol dehydrogenase

Gene

adhE

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 3 (29 Aug 2001)
      Previous versions | rss
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    Functioni

    This enzyme has probably two activities: ADH, and ACDH.

    Catalytic activityi

    An alcohol + NAD+ = an aldehyde or ketone + NADH.
    Acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei244 – 2441By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi420 – 4256NADSequence Analysis

    GO - Molecular functioni

    1. acetaldehyde dehydrogenase (acetylating) activity Source: UniProtKB-EC
    2. alcohol dehydrogenase (NAD) activity Source: CACAO
    3. metal ion binding Source: InterPro

    GO - Biological processi

    1. alcohol metabolic process Source: InterPro
    2. carbon utilization Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciCACE272562:GJIH-4007-MONOMER.
    MetaCyc:AADCLOS-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aldehyde-alcohol dehydrogenase
    Including the following 2 domains:
    Alcohol dehydrogenase (EC:1.1.1.1)
    Short name:
    ADH
    Acetaldehyde dehydrogenase [acetylating] (EC:1.2.1.10)
    Short name:
    ACDH
    Gene namesi
    Name:adhE
    Synonyms:aad
    Ordered Locus Names:CA_P0162
    Encoded oniPlasmid pSOL10 Publication
    OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
    Taxonomic identifieri272562 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000814: Plasmid pSOL1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 862862Aldehyde-alcohol dehydrogenasePRO_0000087839Add
    BLAST

    Expressioni

    Inductioni

    Induced during switch to solvent production.

    Interactioni

    Protein-protein interaction databases

    STRINGi272562.CA_P0162.

    Structurei

    3D structure databases

    ProteinModelPortaliP33744.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    In the N-terminal section; belongs to the aldehyde dehydrogenase family.Curated
    In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiCOG1012.
    HOGENOMiHOG000025256.
    KOiK04072.
    OMAiTHICNEG.
    OrthoDBiEOG6TFCQS.

    Family and domain databases

    Gene3Di3.40.309.10. 2 hits.
    3.40.605.10. 1 hit.
    InterProiIPR001670. ADH_Fe.
    IPR018211. ADH_Fe_CS.
    IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR012079. Bifunc_Ald-ADH.
    [Graphical view]
    PfamiPF00171. Aldedh. 1 hit.
    PF00465. Fe-ADH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000111. ALDH_ADH. 1 hit.
    SUPFAMiSSF53720. SSF53720. 1 hit.
    PROSITEiPS00913. ADH_IRON_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33744-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVTTVKELD EKLKVIKEAQ KKFSCYSQEM VDEIFRNAAM AAIDARIELA    50
    KAAVLETGMG LVEDKVIKNH FAGEYIYNKY KDEKTCGIIE RNEPYGITKI 100
    AEPIGVVAAI IPVTNPTSTT IFKSLISLKT RNGIFFSPHP RAKKSTILAA 150
    KTILDAAVKS GAPENIIGWI DEPSIELTQY LMQKADITLA TGGPSLVKSA 200
    YSSGKPAIGV GPGNTPVIID ESAHIKMAVS SIILSKTYDN GVICASEQSV 250
    IVLKSIYNKV KDEFQERGAY IIKKNELDKV REVIFKDGSV NPKIVGQSAY 300
    TIAAMAGIKV PKTTRILIGE VTSLGEEEPF AHEKLSPVLA MYEADNFDDA 350
    LKKAVTLINL GGLGHTSGIY ADEIKARDKI DRFSSAMKTV RTFVNIPTSQ 400
    GASGDLYNFR IPPSFTLGCG FWGGNSVSEN VGPKHLLNIK TVAERRENML 450
    WFRVPHKVYF KFGCLQFALK DLKDLKKKRA FIVTDSDPYN LNYVDSIIKI 500
    LEHLDIDFKV FNKVGREADL KTIKKATEEM SSFMPDTIIA LGGTPEMSSA 550
    KLMWVLYEHP EVKFEDLAIK FMDIRKRIYT FPKLGKKAML VAITTSAGSG 600
    SEVTPFALVT DNNTGNKYML ADYEMTPNMA IVDAELMMKM PKGLTAYSGI 650
    DALVNSIEAY TSVYASEYTN GLALEAIRLI FKYLPEAYKN GRTNEKAREK 700
    MAHASTMAGM ASANAFLGLC HSMAIKLSSE HNIPSGIANA LLIEEVIKFN 750
    AVDNPVKQAP CPQYKYPNTI FRYARIADYI KLGGNTDEEK VDLLINKIHE 800
    LKKALNIPTS IKDAGVLEEN FYSSLDRISE LALDDQCTGA NPRFPLTSEI 850
    KEMYINCFKK QP 862
    Length:862
    Mass (Da):95,321
    Last modified:August 29, 2001 - v3
    Checksum:iBE09E32B28DD08B0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72831 Genomic DNA. Translation: CAA51344.1.
    L14817 Genomic DNA. Translation: AAD04638.1.
    AE001438 Genomic DNA. Translation: AAK76907.1.
    PIRiA49346.
    RefSeqiNP_149325.1. NC_001988.2.
    WP_010890846.1. NC_001988.2.

    Genome annotation databases

    EnsemblBacteriaiAAK76907; AAK76907; CA_P0162.
    GeneIDi1116167.
    KEGGicac:CA_P0162.
    PATRICi32034210. VBICloAce74127_0163.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72831 Genomic DNA. Translation: CAA51344.1 .
    L14817 Genomic DNA. Translation: AAD04638.1 .
    AE001438 Genomic DNA. Translation: AAK76907.1 .
    PIRi A49346.
    RefSeqi NP_149325.1. NC_001988.2.
    WP_010890846.1. NC_001988.2.

    3D structure databases

    ProteinModelPortali P33744.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272562.CA_P0162.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK76907 ; AAK76907 ; CA_P0162 .
    GeneIDi 1116167.
    KEGGi cac:CA_P0162.
    PATRICi 32034210. VBICloAce74127_0163.

    Phylogenomic databases

    eggNOGi COG1012.
    HOGENOMi HOG000025256.
    KOi K04072.
    OMAi THICNEG.
    OrthoDBi EOG6TFCQS.

    Enzyme and pathway databases

    BioCyci CACE272562:GJIH-4007-MONOMER.
    MetaCyc:AADCLOS-MONOMER.

    Family and domain databases

    Gene3Di 3.40.309.10. 2 hits.
    3.40.605.10. 1 hit.
    InterProi IPR001670. ADH_Fe.
    IPR018211. ADH_Fe_CS.
    IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR012079. Bifunc_Ald-ADH.
    [Graphical view ]
    Pfami PF00171. Aldedh. 1 hit.
    PF00465. Fe-ADH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000111. ALDH_ADH. 1 hit.
    SUPFAMi SSF53720. SSF53720. 1 hit.
    PROSITEi PS00913. ADH_IRON_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis."
      Fischer R.J., Helms J., Duerre P.
      J. Bacteriol. 175:6959-6969(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
    2. "Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824."
      Nair R.V., Bennett G.N., Papoutsakis E.T.
      J. Bacteriol. 176:871-885(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

    Entry informationi

    Entry nameiADHE_CLOAB
    AccessioniPrimary (citable) accession number: P33744
    Secondary accession number(s): Q45808, Q45809
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: August 29, 2001
    Last modified: October 1, 2014
    This is version 97 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Plasmid, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3