P33727 (ARSB_FELCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arylsulfatase B
Short name=ASB
EC=3.1.6.12

Alternative name(s):
N-acetylgalactosamine-4-sulfatase
Short name=G4S

Gene names

Name:

ARSB

Organism

Felis catus (Cat) (Felis silvestris catus)

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of the 4-sulfate groups of the N-acetyl-D-galactosamine 4-sulfate units of chondroitin sulfate and dermatan sulfate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Homodimer.
Subcellular location	Lysosome.
Post-translational modification	The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity By similarity.
Involvement in disease	Note=Defects in ARSB are the cause of mucopolysaccharidosis type VI (MPS-VI). MPS-VI has been described in Siamese cats.
Sequence similarities	Belongs to the sulfatase family.

Ontologies

Keywords
Cellular component	Lysosome
Disease	Mucopolysaccharidosis
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylgalactosamine-4-sulfatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 41	41	Potential
	Chain	42 – 535	494	Arylsulfatase B		PRO_0000033422
Sites
	Active site	149	1	By similarity
	Metal binding	55	1	Calcium By similarity
	Metal binding	56	1	Calcium By similarity
	Metal binding	93	1	Calcium; via 3-oxoalanine By similarity
	Metal binding	302	1	Calcium By similarity
	Metal binding	303	1	Calcium By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	320	1	Substrate By similarity
Amino acid modifications
	Modified residue	93	1	3-oxoalanine (Cys) By similarity
	Glycosylation	190	1	N-linked (GlcNAc...) Potential
	Glycosylation	281	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	428	1	N-linked (GlcNAc...) Potential
	Glycosylation	460	1	N-linked (GlcNAc...) Potential
	Disulfide bond	119 ↔ 523		By similarity
	Disulfide bond	123 ↔ 157		By similarity
	Disulfide bond	183 ↔ 194		By similarity
	Disulfide bond	407 ↔ 449		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33727 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 43A527886A9983C4	FASTA	535	59,753
	10 20 30 40 50 60 MGRRGAASLP RGPSPRRPLL PGVLPLLLRL LLLPSRPGAG AGADRPPHLV FVLADDLGWN 70 80 90 100 110 120 DVSFHGSNIR TPHLDELAAG GVLLDNYYTQ PLCTPSRSQL LTGRYQIHTG LQHQIIWPCQ 130 140 150 160 170 180 PSCVPLDEKL LPQLLKEAGY TTHMVGKWHL GMYRKECLPT RRGFDTYFGY LLGSEDYYSH 190 200 210 220 230 240 ERCALIDSLN VTRCALDFRD GEQVATGYKN MYSTNIFTER ATALITSHPP EKPLFLYLAL 250 260 270 280 290 300 QSVHEPLQVP EEYLKPYDFI QDKNRHYYAG MVSLMDEAVG NVTAALKSHG LWNNTVFIFS 310 320 330 340 350 360 TDNGGQTLAG GNNWPLRGRK WSLWEGGIRG VGFVASPLLK QKGVKNRELI HISDWLPTLV 370 380 390 400 410 420 KLARGSTKGT KPLDGFDVWK TISEGSPSPR KELLHNIDPN FVDISPCPGK SLAPAKDDSS 430 440 450 460 470 480 HPAYLAFNTS LHAAIRHGNW KLLTGYPGCG CWFPPPSPYN DSAIPSSDPP TKTLWPFDID 490 500 510 520 530 QDPEERHDLS RDYPHIVEQL LSRLQFYHKH SVPVHFPAQD PRCDPKGTGA WGPWV

References

[1]	"Feline arylsulfatase B (ARSB): isolation and expression of the cDNA, comparison with human ARSB, and gene localization to feline chromosome A1." Jackson C.E., Yuhki N., Desnick R.J., Haskins M.E., O'Brien S.J., Schuchman E.H. Genomics 14:403-411(1992) [PubMed: 1427856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S48472 mRNA. Translation: AAB23941.1.
PIR	A44475.
RefSeq	NP_001135731.1. NM_001142259.1.
3D structure databases
ProteinModelPortal	P33727.
SMR	P33727. Positions 45-535.
ModBase	Search...
Protein-protein interaction databases
STRING	P33727.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100216331.
Organism-specific databases
CTD	411.
Phylogenomic databases
eggNOG	maNOG08183.
HOVERGEN	HBG004282.
Family and domain databases
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR017850. Alkaline_phosphatase_core. IPR000917. Sulfatase. IPR024607. Sulfatase_CS. [Graphical view]
Gene3D	G3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
Pfam	PF00884. Sulfatase. 1 hit. [Graphical view]
SUPFAM	SSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITE	PS00523. SULFATASE_1. 1 hit. PS00149. SULFATASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARSB_FELCA

Accession

Primary (citable) accession number: P33727

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	October 19, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program