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Reviewed, UniProtKB/Swiss-Prot P33727 (ARSB_FELCA)

Last modified January 19, 2010. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Arylsulfatase B
      Short name=ASB
    EC=3.1.6.12
Alternative name(s):
    N-acetylgalactosamine-4-sulfatase
      Short name=G4S
Gene names
Name: ARSB
OrganismFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifier9685 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

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Protein attributes

Sequence length535 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

Hydrolysis of the 4-sulfate groups of the N-acetyl-D-galactosamine 4-sulfate units of chondroitin sulfate and dermatan sulfate.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subunit structure

Homodimer.

Subcellular location

Lysosome.

Post-translational modification

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity By similarity.

Involvement in disease

Defects in ARSB are the cause of mucopolysaccharidosis type VI (MPS-VI). MPS-VI has been described in Siamese cats.

Sequence similarities

Belongs to the sulfatase family.

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Ontologies

Keywords
   Cellular componentLysosome
   DiseaseMucopolysaccharidosis
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionN-acetylgalactosamine-4-sulfatase activity

Inferred from electronic annotation. Source: EC

calcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4141 Potential
Chain42 – 535494Arylsulfatase B
PRO_0000033422

Sites

Active site1491 By similarity
Metal binding551Calcium By similarity
Metal binding561Calcium By similarity
Metal binding931Calcium; via 3-oxoalanine By similarity
Metal binding3021Calcium By similarity
Metal binding3031Calcium By similarity
Binding site1471Substrate By similarity
Binding site2441Substrate By similarity
Binding site3201Substrate By similarity

Amino acid modifications

Modified residue9313-oxoalanine (Cys) By similarity
Glycosylation1901N-linked (GlcNAc...) Potential
Glycosylation2811N-linked (GlcNAc...) Potential
Glycosylation2931N-linked (GlcNAc...) Potential
Glycosylation4281N-linked (GlcNAc...) Potential
Glycosylation4601N-linked (GlcNAc...) Potential
Disulfide bond119 ↔ 523 By similarity
Disulfide bond123 ↔ 157 By similarity
Disulfide bond183 ↔ 194 By similarity
Disulfide bond407 ↔ 449 By similarity

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Sequences

Sequence LengthMass (Da)Tools
P33727-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 43A527886A9983C4

FASTA53559,753
        10         20         30         40         50         60 
MGRRGAASLP RGPSPRRPLL PGVLPLLLRL LLLPSRPGAG AGADRPPHLV FVLADDLGWN 

        70         80         90        100        110        120 
DVSFHGSNIR TPHLDELAAG GVLLDNYYTQ PLCTPSRSQL LTGRYQIHTG LQHQIIWPCQ 

       130        140        150        160        170        180 
PSCVPLDEKL LPQLLKEAGY TTHMVGKWHL GMYRKECLPT RRGFDTYFGY LLGSEDYYSH 

       190        200        210        220        230        240 
ERCALIDSLN VTRCALDFRD GEQVATGYKN MYSTNIFTER ATALITSHPP EKPLFLYLAL 

       250        260        270        280        290        300 
QSVHEPLQVP EEYLKPYDFI QDKNRHYYAG MVSLMDEAVG NVTAALKSHG LWNNTVFIFS 

       310        320        330        340        350        360 
TDNGGQTLAG GNNWPLRGRK WSLWEGGIRG VGFVASPLLK QKGVKNRELI HISDWLPTLV 

       370        380        390        400        410        420 
KLARGSTKGT KPLDGFDVWK TISEGSPSPR KELLHNIDPN FVDISPCPGK SLAPAKDDSS 

       430        440        450        460        470        480 
HPAYLAFNTS LHAAIRHGNW KLLTGYPGCG CWFPPPSPYN DSAIPSSDPP TKTLWPFDID 

       490        500        510        520        530 
QDPEERHDLS RDYPHIVEQL LSRLQFYHKH SVPVHFPAQD PRCDPKGTGA WGPWV

« Hide

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References

[1]"Feline arylsulfatase B (ARSB): isolation and expression of the cDNA, comparison with human ARSB, and gene localization to feline chromosome A1."
Jackson C.E., Yuhki N., Desnick R.J., Haskins M.E., O'Brien S.J., Schuchman E.H.
Genomics 14:403-411(1992) [PubMed: 1427856] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		S48472 mRNA. Translation: AAB23941.1.
PIRA44475.
RefSeqNP_001135731.1.

3D structure databases

SMRP33727. Positions 45-535.
ModBaseSearch...

Protein-protein interaction databases

STRINGP33727.

Genome annotation databases

GeneID100216331.

Organism-specific databases

CTD100216331.

Phylogenomic databases

eggNOGmaNOG08183.
HOVERGENP33727.

Enzyme and pathway databases

BRENDA3.1.6.12. 273949.

Family and domain databases

InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR000917. Sulfatase.
[Graphical view]
Gene3DG3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
PfamPF00884. Sulfatase. 1 hit.
[Graphical view]
PROSITEPS00523. SULFATASE_1. 1 hit.
PS00149. SULFATASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameARSB_FELCA
AccessionPrimary (citable) accession number: P33727
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 19, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents