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P33726 (PDE6B_CANFA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta

Short name=GMP-PDE beta
EC=3.1.4.35
Gene names
Name:PDE6B
Synonyms:PDBS, PDEB
OrganismCanis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]
Taxonomic identifier9615 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis

Protein attributes

Sequence length856 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.

Catalytic activity

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Subunit structure

Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

Subcellular location

Membrane; Lipid-anchor.

Involvement in disease

Irish setter dogs affected with rod/cone dysplasia (RCD1) contain a nonsense mutation in the gene that gives rise to a protein of 807 AA lacking 49 AA in the C-terminal.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Contains 2 GAF domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 853852Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
PRO_0000023346
Propeptide854 – 8563Removed in mature form By similarity
PRO_0000023347

Regions

Domain71 – 220150GAF 1
Domain252 – 429178GAF 2

Sites

Active site5571Proton donor By similarity
Metal binding5611Divalent metal cation 1 By similarity
Metal binding5971Divalent metal cation 1 By similarity
Metal binding5981Divalent metal cation 1 By similarity
Metal binding5981Divalent metal cation 2 By similarity
Metal binding7181Divalent metal cation 1 By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Lipidation8531S-geranylgeranyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
P33726 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: AC9D03F64D1BA132

FASTA85698,462
        10         20         30         40         50         60 
MSLSEEQVQH FLDQNPDFTD QYFGKTLSPE HVAGACGDGQ PTDCASFREL CQVEESAALF 

        70         80         90        100        110        120 
ELVQDMQESV NMERVVFKIL RRLCTILRAD RCSLFMYRQR NGVAELATRL FSVQPGSALE 

       130        140        150        160        170        180 
DCLVPPDSEI VFPLDIGVVG HVAQTKKMVN VQDVTECPHF SPFADELTGY ETRNILATPI 

       190        200        210        220        230        240 
MNGKDVVAVI MALNKLDGPC FTSEDEDVFL KYLNFGTLNL KIYHLSYLHN CETRRGQVLL 

       250        260        270        280        290        300 
WSANKVFEEL TDIERQFHKA FYTVRAYLNC DRYSVGLLDM TKEKEFFDVW PVLMGEAQPY 

       310        320        330        340        350        360 
SGPRTPDGRE IVFYKVIDYI LHGKEDIKVI PSPPADHWAL ASGLPTYVAE SGFICNIMNT 

       370        380        390        400        410        420 
AADEMFTFQE GPLDDSGWVI KNVLSMPIVN KKEEIVGVAT FYNRKDGKPF DEQDEVLMES 

       430        440        450        460        470        480 
LTQFLGWSVL NTDTYDKMNK LENRKDIAQD MVLYHVRCDK DEIQLILPTR ERLGKEPADC 

       490        500        510        520        530        540 
EEDELGILLK EVLPGPSKFD IYEFHFSDLE CTELELVKCG IQMYYELGVV RKFQIPQEVL 

       550        560        570        580        590        600 
VRFLFSVSKG YRRITYHNWR HGFNVAQTMF TLLTTGKLKS YYTDLEAFAM VTAGLCHDID 

       610        620        630        640        650        660 
HRGTNNLYQM KSQNPLAKLH GSSILERHHL EFGKFLLSEE TLNIYQNLNR RQHEHVIHLM 

       670        680        690        700        710        720 
DIAIIATDLA LYFKKRTMFQ KIVDESKNYE DRKSWVEYLS LETTRKEIVM AMMMTACDLS 

       730        740        750        760        770        780 
AITKPWEVQS KVALLVAAEF WEQGDLERTV LDQQPIPMMD RNKAAELPKL QVGFIDFVCT 

       790        800        810        820        830        840 
FVYKEFSRFH EEILPMFDRL QNNRKEWKAL ADEYEAKLKA LEEEKQQQED RTTAKKAGTE 

       850 
ICNGGPAPKS STCCIL 

« Hide

References

[1]"Irish setter dogs affected with rod/cone dysplasia contain a nonsense mutation in the rod cGMP phosphodiesterase beta-subunit gene."
Suber M.L., Pittler S.J., Qin N., Wright G.C., Holcombe V., Lee R.H., Craft C.M., Lolley R.N., Baehr W.B., Hurwitz R.L.
Proc. Natl. Acad. Sci. U.S.A. 90:3968-3972(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Red setter.
Tissue: Retina.
[2]Clements P.J.
Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Boxer X Doberman.
Tissue: Retina.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z23014 mRNA. Translation: CAA80557.1.
L13262 mRNA. Translation: AAA30882.1. Sequence problems.
PIRA47451.
RefSeqNP_001002934.1. NM_001002934.1.
UniGeneCfa.3793.

3D structure databases

ProteinModelPortalP33726.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9615.ENSCAFP00000031337.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSCAFT00000026577; ENSCAFP00000024683; ENSCAFG00000016782.
GeneID399653.
KEGGcfa:399653.

Organism-specific databases

CTD5158.

Phylogenomic databases

eggNOGNOG242608.
GeneTreeENSGT00750000117253.
HOGENOMHOG000007069.
HOVERGENHBG053539.
InParanoidP33726.
KOK13756.
OMAGVVKKFQ.
OrthoDBEOG7BGHK1.
TreeFamTF316499.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMSSF55781. SSF55781. 3 hits.
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20816613.

Entry information

Entry namePDE6B_CANFA
AccessionPrimary (citable) accession number: P33726
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families