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P33726

- PDE6B_CANFA

UniProt

P33726 - PDE6B_CANFA

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Protein
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
Gene
PDE6B, PDBS, PDEB
Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei557 – 5571Proton donor By similarity
Metal bindingi561 – 5611Divalent metal cation 1 By similarity
Metal bindingi597 – 5971Divalent metal cation 1 By similarity
Metal bindingi598 – 5981Divalent metal cation 1 By similarity
Metal bindingi598 – 5981Divalent metal cation 2 By similarity
Metal bindingi718 – 7181Divalent metal cation 1 By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. GMP metabolic process Source: Ensembl
  2. cytosolic calcium ion homeostasis Source: Ensembl
  3. detection of light stimulus Source: Ensembl
  4. retina development in camera-type eye Source: Ensembl
  5. signal transduction Source: InterPro
  6. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_174228. Activation of the phototransduction cascade.
REACT_174305. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_175273. Ca2+ pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta (EC:3.1.4.35)
Short name:
GMP-PDE beta
Gene namesi
Name:PDE6B
Synonyms:PDBS, PDEB
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Chromosome 3

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Irish setter dogs affected with rod/cone dysplasia (RCD1) contain a nonsense mutation in the gene that gives rise to a protein of 807 AA lacking 49 AA in the C-terminal.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 853852Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
PRO_0000023346Add
BLAST
Propeptidei854 – 8563Removed in mature form By similarity
PRO_0000023347

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Lipidationi853 – 8531S-geranylgeranyl cysteine By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Prenylation

Interactioni

Subunit structurei

Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000031337.

Structurei

3D structure databases

ProteinModelPortaliP33726.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini71 – 220150GAF 1
Add
BLAST
Domaini252 – 429178GAF 2
Add
BLAST

Sequence similaritiesi

Contains 2 GAF domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG242608.
GeneTreeiENSGT00750000117253.
HOGENOMiHOG000007069.
HOVERGENiHBG053539.
InParanoidiP33726.
KOiK13756.
OMAiGVVKKFQ.
OrthoDBiEOG7BGHK1.
TreeFamiTF316499.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33726-1 [UniParc]FASTAAdd to Basket

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MSLSEEQVQH FLDQNPDFTD QYFGKTLSPE HVAGACGDGQ PTDCASFREL    50
CQVEESAALF ELVQDMQESV NMERVVFKIL RRLCTILRAD RCSLFMYRQR 100
NGVAELATRL FSVQPGSALE DCLVPPDSEI VFPLDIGVVG HVAQTKKMVN 150
VQDVTECPHF SPFADELTGY ETRNILATPI MNGKDVVAVI MALNKLDGPC 200
FTSEDEDVFL KYLNFGTLNL KIYHLSYLHN CETRRGQVLL WSANKVFEEL 250
TDIERQFHKA FYTVRAYLNC DRYSVGLLDM TKEKEFFDVW PVLMGEAQPY 300
SGPRTPDGRE IVFYKVIDYI LHGKEDIKVI PSPPADHWAL ASGLPTYVAE 350
SGFICNIMNT AADEMFTFQE GPLDDSGWVI KNVLSMPIVN KKEEIVGVAT 400
FYNRKDGKPF DEQDEVLMES LTQFLGWSVL NTDTYDKMNK LENRKDIAQD 450
MVLYHVRCDK DEIQLILPTR ERLGKEPADC EEDELGILLK EVLPGPSKFD 500
IYEFHFSDLE CTELELVKCG IQMYYELGVV RKFQIPQEVL VRFLFSVSKG 550
YRRITYHNWR HGFNVAQTMF TLLTTGKLKS YYTDLEAFAM VTAGLCHDID 600
HRGTNNLYQM KSQNPLAKLH GSSILERHHL EFGKFLLSEE TLNIYQNLNR 650
RQHEHVIHLM DIAIIATDLA LYFKKRTMFQ KIVDESKNYE DRKSWVEYLS 700
LETTRKEIVM AMMMTACDLS AITKPWEVQS KVALLVAAEF WEQGDLERTV 750
LDQQPIPMMD RNKAAELPKL QVGFIDFVCT FVYKEFSRFH EEILPMFDRL 800
QNNRKEWKAL ADEYEAKLKA LEEEKQQQED RTTAKKAGTE ICNGGPAPKS 850
STCCIL 856
Length:856
Mass (Da):98,462
Last modified:February 1, 1994 - v1
Checksum:iAC9D03F64D1BA132
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z23014 mRNA. Translation: CAA80557.1.
L13262 mRNA. Translation: AAA30882.1. Sequence problems.
PIRiA47451.
RefSeqiNP_001002934.1. NM_001002934.1.
UniGeneiCfa.3793.

Genome annotation databases

EnsembliENSCAFT00000026577; ENSCAFP00000024683; ENSCAFG00000016782.
GeneIDi399653.
KEGGicfa:399653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z23014 mRNA. Translation: CAA80557.1 .
L13262 mRNA. Translation: AAA30882.1 . Sequence problems.
PIRi A47451.
RefSeqi NP_001002934.1. NM_001002934.1.
UniGenei Cfa.3793.

3D structure databases

ProteinModelPortali P33726.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9615.ENSCAFP00000031337.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSCAFT00000026577 ; ENSCAFP00000024683 ; ENSCAFG00000016782 .
GeneIDi 399653.
KEGGi cfa:399653.

Organism-specific databases

CTDi 5158.

Phylogenomic databases

eggNOGi NOG242608.
GeneTreei ENSGT00750000117253.
HOGENOMi HOG000007069.
HOVERGENi HBG053539.
InParanoidi P33726.
KOi K13756.
OMAi GVVKKFQ.
OrthoDBi EOG7BGHK1.
TreeFami TF316499.

Enzyme and pathway databases

Reactomei REACT_174228. Activation of the phototransduction cascade.
REACT_174305. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_175273. Ca2+ pathway.

Miscellaneous databases

NextBioi 20816613.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProi IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view ]
SUPFAMi SSF55781. SSF55781. 3 hits.
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Irish setter dogs affected with rod/cone dysplasia contain a nonsense mutation in the rod cGMP phosphodiesterase beta-subunit gene."
    Suber M.L., Pittler S.J., Qin N., Wright G.C., Holcombe V., Lee R.H., Craft C.M., Lolley R.N., Baehr W.B., Hurwitz R.L.
    Proc. Natl. Acad. Sci. U.S.A. 90:3968-3972(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Red setter.
    Tissue: Retina.
  2. Clements P.J.
    Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Boxer X Doberman.
    Tissue: Retina.

Entry informationi

Entry nameiPDE6B_CANFA
AccessioniPrimary (citable) accession number: P33726
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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