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Protein

Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta

Gene

PDE6B

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei557 – 5571Proton donorBy similarity
Metal bindingi561 – 5611Divalent metal cation 1By similarity
Metal bindingi597 – 5971Divalent metal cation 1By similarity
Metal bindingi598 – 5981Divalent metal cation 1By similarity
Metal bindingi598 – 5981Divalent metal cation 2By similarity
Metal bindingi718 – 7181Divalent metal cation 1By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_313434. Activation of the phototransduction cascade.
REACT_324552. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_346553. Ca2+ pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta (EC:3.1.4.35)
Short name:
GMP-PDE beta
Gene namesi
Name:PDE6B
Synonyms:PDBS, PDEB
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Irish setter dogs affected with rod/cone dysplasia (RCD1) contain a nonsense mutation in the gene that gives rise to a protein of 807 AA lacking 49 AA in the C-terminal.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 853852Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit betaPRO_0000023346Add
BLAST
Propeptidei854 – 8563Removed in mature formBy similarityPRO_0000023347

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Lipidationi853 – 8531S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Prenylation

Interactioni

Subunit structurei

Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000024683.

Structurei

3D structure databases

ProteinModelPortaliP33726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini71 – 220150GAF 1Add
BLAST
Domaini252 – 429178GAF 2Add
BLAST

Sequence similaritiesi

Contains 2 GAF domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG242608.
GeneTreeiENSGT00760000119066.
HOGENOMiHOG000007069.
HOVERGENiHBG053539.
InParanoidiP33726.
KOiK13756.
OMAiGVVKKFQ.
OrthoDBiEOG7BGHK1.
TreeFamiTF316499.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSEEQVQH FLDQNPDFTD QYFGKTLSPE HVAGACGDGQ PTDCASFREL
60 70 80 90 100
CQVEESAALF ELVQDMQESV NMERVVFKIL RRLCTILRAD RCSLFMYRQR
110 120 130 140 150
NGVAELATRL FSVQPGSALE DCLVPPDSEI VFPLDIGVVG HVAQTKKMVN
160 170 180 190 200
VQDVTECPHF SPFADELTGY ETRNILATPI MNGKDVVAVI MALNKLDGPC
210 220 230 240 250
FTSEDEDVFL KYLNFGTLNL KIYHLSYLHN CETRRGQVLL WSANKVFEEL
260 270 280 290 300
TDIERQFHKA FYTVRAYLNC DRYSVGLLDM TKEKEFFDVW PVLMGEAQPY
310 320 330 340 350
SGPRTPDGRE IVFYKVIDYI LHGKEDIKVI PSPPADHWAL ASGLPTYVAE
360 370 380 390 400
SGFICNIMNT AADEMFTFQE GPLDDSGWVI KNVLSMPIVN KKEEIVGVAT
410 420 430 440 450
FYNRKDGKPF DEQDEVLMES LTQFLGWSVL NTDTYDKMNK LENRKDIAQD
460 470 480 490 500
MVLYHVRCDK DEIQLILPTR ERLGKEPADC EEDELGILLK EVLPGPSKFD
510 520 530 540 550
IYEFHFSDLE CTELELVKCG IQMYYELGVV RKFQIPQEVL VRFLFSVSKG
560 570 580 590 600
YRRITYHNWR HGFNVAQTMF TLLTTGKLKS YYTDLEAFAM VTAGLCHDID
610 620 630 640 650
HRGTNNLYQM KSQNPLAKLH GSSILERHHL EFGKFLLSEE TLNIYQNLNR
660 670 680 690 700
RQHEHVIHLM DIAIIATDLA LYFKKRTMFQ KIVDESKNYE DRKSWVEYLS
710 720 730 740 750
LETTRKEIVM AMMMTACDLS AITKPWEVQS KVALLVAAEF WEQGDLERTV
760 770 780 790 800
LDQQPIPMMD RNKAAELPKL QVGFIDFVCT FVYKEFSRFH EEILPMFDRL
810 820 830 840 850
QNNRKEWKAL ADEYEAKLKA LEEEKQQQED RTTAKKAGTE ICNGGPAPKS

STCCIL
Length:856
Mass (Da):98,462
Last modified:February 1, 1994 - v1
Checksum:iAC9D03F64D1BA132
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23014 mRNA. Translation: CAA80557.1.
L13262 mRNA. Translation: AAA30882.1. Sequence problems.
PIRiA47451.
RefSeqiNP_001002934.1. NM_001002934.1.
UniGeneiCfa.3793.

Genome annotation databases

EnsembliENSCAFT00000026577; ENSCAFP00000024683; ENSCAFG00000016782.
GeneIDi399653.
KEGGicfa:399653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z23014 mRNA. Translation: CAA80557.1.
L13262 mRNA. Translation: AAA30882.1. Sequence problems.
PIRiA47451.
RefSeqiNP_001002934.1. NM_001002934.1.
UniGeneiCfa.3793.

3D structure databases

ProteinModelPortaliP33726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000024683.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000026577; ENSCAFP00000024683; ENSCAFG00000016782.
GeneIDi399653.
KEGGicfa:399653.

Organism-specific databases

CTDi5158.

Phylogenomic databases

eggNOGiNOG242608.
GeneTreeiENSGT00760000119066.
HOGENOMiHOG000007069.
HOVERGENiHBG053539.
InParanoidiP33726.
KOiK13756.
OMAiGVVKKFQ.
OrthoDBiEOG7BGHK1.
TreeFamiTF316499.

Enzyme and pathway databases

ReactomeiREACT_313434. Activation of the phototransduction cascade.
REACT_324552. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_346553. Ca2+ pathway.

Miscellaneous databases

NextBioi20816613.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Irish setter dogs affected with rod/cone dysplasia contain a nonsense mutation in the rod cGMP phosphodiesterase beta-subunit gene."
    Suber M.L., Pittler S.J., Qin N., Wright G.C., Holcombe V., Lee R.H., Craft C.M., Lolley R.N., Baehr W.B., Hurwitz R.L.
    Proc. Natl. Acad. Sci. U.S.A. 90:3968-3972(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Red setter.
    Tissue: Retina.
  2. Clements P.J.
    Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Boxer X Doberman.
    Tissue: Retina.

Entry informationi

Entry nameiPDE6B_CANFA
AccessioniPrimary (citable) accession number: P33726
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 24, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.