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P33726

- PDE6B_CANFA

UniProt

P33726 - PDE6B_CANFA

Protein

Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta

Gene

PDE6B

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.

    Catalytic activityi

    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei557 – 5571Proton donorBy similarity
    Metal bindingi561 – 5611Divalent metal cation 1By similarity
    Metal bindingi597 – 5971Divalent metal cation 1By similarity
    Metal bindingi598 – 5981Divalent metal cation 1By similarity
    Metal bindingi598 – 5981Divalent metal cation 2By similarity
    Metal bindingi718 – 7181Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cytosolic calcium ion homeostasis Source: Ensembl
    2. detection of light stimulus Source: Ensembl
    3. GMP metabolic process Source: Ensembl
    4. retina development in camera-type eye Source: Ensembl
    5. signal transduction Source: InterPro
    6. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    cGMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_174228. Activation of the phototransduction cascade.
    REACT_174305. Inactivation, recovery and regulation of the phototransduction cascade.
    REACT_175273. Ca2+ pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta (EC:3.1.4.35)
    Short name:
    GMP-PDE beta
    Gene namesi
    Name:PDE6B
    Synonyms:PDBS, PDEB
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Chromosome 3

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Irish setter dogs affected with rod/cone dysplasia (RCD1) contain a nonsense mutation in the gene that gives rise to a protein of 807 AA lacking 49 AA in the C-terminal.

    Keywords - Diseasei

    Disease mutation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 853852Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit betaPRO_0000023346Add
    BLAST
    Propeptidei854 – 8563Removed in mature formBy similarityPRO_0000023347

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Lipidationi853 – 8531S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Prenylation

    Interactioni

    Subunit structurei

    Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

    Protein-protein interaction databases

    STRINGi9615.ENSCAFP00000031337.

    Structurei

    3D structure databases

    ProteinModelPortaliP33726.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini71 – 220150GAF 1Add
    BLAST
    Domaini252 – 429178GAF 2Add
    BLAST

    Sequence similaritiesi

    Contains 2 GAF domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG242608.
    GeneTreeiENSGT00750000117253.
    HOGENOMiHOG000007069.
    HOVERGENiHBG053539.
    InParanoidiP33726.
    KOiK13756.
    OMAiGVVKKFQ.
    OrthoDBiEOG7BGHK1.
    TreeFamiTF316499.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 3 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33726-1 [UniParc]FASTAAdd to Basket

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    MSLSEEQVQH FLDQNPDFTD QYFGKTLSPE HVAGACGDGQ PTDCASFREL    50
    CQVEESAALF ELVQDMQESV NMERVVFKIL RRLCTILRAD RCSLFMYRQR 100
    NGVAELATRL FSVQPGSALE DCLVPPDSEI VFPLDIGVVG HVAQTKKMVN 150
    VQDVTECPHF SPFADELTGY ETRNILATPI MNGKDVVAVI MALNKLDGPC 200
    FTSEDEDVFL KYLNFGTLNL KIYHLSYLHN CETRRGQVLL WSANKVFEEL 250
    TDIERQFHKA FYTVRAYLNC DRYSVGLLDM TKEKEFFDVW PVLMGEAQPY 300
    SGPRTPDGRE IVFYKVIDYI LHGKEDIKVI PSPPADHWAL ASGLPTYVAE 350
    SGFICNIMNT AADEMFTFQE GPLDDSGWVI KNVLSMPIVN KKEEIVGVAT 400
    FYNRKDGKPF DEQDEVLMES LTQFLGWSVL NTDTYDKMNK LENRKDIAQD 450
    MVLYHVRCDK DEIQLILPTR ERLGKEPADC EEDELGILLK EVLPGPSKFD 500
    IYEFHFSDLE CTELELVKCG IQMYYELGVV RKFQIPQEVL VRFLFSVSKG 550
    YRRITYHNWR HGFNVAQTMF TLLTTGKLKS YYTDLEAFAM VTAGLCHDID 600
    HRGTNNLYQM KSQNPLAKLH GSSILERHHL EFGKFLLSEE TLNIYQNLNR 650
    RQHEHVIHLM DIAIIATDLA LYFKKRTMFQ KIVDESKNYE DRKSWVEYLS 700
    LETTRKEIVM AMMMTACDLS AITKPWEVQS KVALLVAAEF WEQGDLERTV 750
    LDQQPIPMMD RNKAAELPKL QVGFIDFVCT FVYKEFSRFH EEILPMFDRL 800
    QNNRKEWKAL ADEYEAKLKA LEEEKQQQED RTTAKKAGTE ICNGGPAPKS 850
    STCCIL 856
    Length:856
    Mass (Da):98,462
    Last modified:February 1, 1994 - v1
    Checksum:iAC9D03F64D1BA132
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z23014 mRNA. Translation: CAA80557.1.
    L13262 mRNA. Translation: AAA30882.1. Sequence problems.
    PIRiA47451.
    RefSeqiNP_001002934.1. NM_001002934.1.
    UniGeneiCfa.3793.

    Genome annotation databases

    EnsembliENSCAFT00000026577; ENSCAFP00000024683; ENSCAFG00000016782.
    GeneIDi399653.
    KEGGicfa:399653.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z23014 mRNA. Translation: CAA80557.1 .
    L13262 mRNA. Translation: AAA30882.1 . Sequence problems.
    PIRi A47451.
    RefSeqi NP_001002934.1. NM_001002934.1.
    UniGenei Cfa.3793.

    3D structure databases

    ProteinModelPortali P33726.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9615.ENSCAFP00000031337.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSCAFT00000026577 ; ENSCAFP00000024683 ; ENSCAFG00000016782 .
    GeneIDi 399653.
    KEGGi cfa:399653.

    Organism-specific databases

    CTDi 5158.

    Phylogenomic databases

    eggNOGi NOG242608.
    GeneTreei ENSGT00750000117253.
    HOGENOMi HOG000007069.
    HOVERGENi HBG053539.
    InParanoidi P33726.
    KOi K13756.
    OMAi GVVKKFQ.
    OrthoDBi EOG7BGHK1.
    TreeFami TF316499.

    Enzyme and pathway databases

    Reactomei REACT_174228. Activation of the phototransduction cascade.
    REACT_174305. Inactivation, recovery and regulation of the phototransduction cascade.
    REACT_175273. Ca2+ pathway.

    Miscellaneous databases

    NextBioi 20816613.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProi IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55781. SSF55781. 3 hits.
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Irish setter dogs affected with rod/cone dysplasia contain a nonsense mutation in the rod cGMP phosphodiesterase beta-subunit gene."
      Suber M.L., Pittler S.J., Qin N., Wright G.C., Holcombe V., Lee R.H., Craft C.M., Lolley R.N., Baehr W.B., Hurwitz R.L.
      Proc. Natl. Acad. Sci. U.S.A. 90:3968-3972(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Red setter.
      Tissue: Retina.
    2. Clements P.J.
      Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Boxer X Doberman.
      Tissue: Retina.

    Entry informationi

    Entry nameiPDE6B_CANFA
    AccessioniPrimary (citable) accession number: P33726
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3