Reviewed, UniProtKB/Swiss-Prot P33726 (PDE6B_CANFA)

Names and origin

Protein names

Recommended name:
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
      Short name=GMP-PDE beta
    EC=3.1.4.35

Gene names

Name:	PDE6B
Synonyms:	PDBS, PDEB

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	856 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Subunit structure	Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.
Subcellular location	Membrane; Lipid-anchor.
Involvement in disease	Irish setter dogs affected with rod/cone dysplasia (RCD1) contain a nonsense mutation in the gene that gives rise to a protein of 807 AA lacking 49 AA in the C-terminal.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Disease	Disease mutation
Domain	Repeat
Ligand	cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Prenylation
Gene Ontology (GO)
Biological process	response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 853	853	Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta		PRO_0000023346
	Propeptide	854 – 856	3	Removed in mature form By similarity		PRO_0000023347
Regions
	Domain	71 – 220	150	GAF 1
	Domain	252 – 429	178	GAF 2
Amino acid modifications
	Lipidation	853	1	S-geranylgeranyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33726-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: AC9D03F64D1BA132	FASTA	856	98,462
	10 20 30 40 50 60 MSLSEEQVQH FLDQNPDFTD QYFGKTLSPE HVAGACGDGQ PTDCASFREL CQVEESAALF 70 80 90 100 110 120 ELVQDMQESV NMERVVFKIL RRLCTILRAD RCSLFMYRQR NGVAELATRL FSVQPGSALE 130 140 150 160 170 180 DCLVPPDSEI VFPLDIGVVG HVAQTKKMVN VQDVTECPHF SPFADELTGY ETRNILATPI 190 200 210 220 230 240 MNGKDVVAVI MALNKLDGPC FTSEDEDVFL KYLNFGTLNL KIYHLSYLHN CETRRGQVLL 250 260 270 280 290 300 WSANKVFEEL TDIERQFHKA FYTVRAYLNC DRYSVGLLDM TKEKEFFDVW PVLMGEAQPY 310 320 330 340 350 360 SGPRTPDGRE IVFYKVIDYI LHGKEDIKVI PSPPADHWAL ASGLPTYVAE SGFICNIMNT 370 380 390 400 410 420 AADEMFTFQE GPLDDSGWVI KNVLSMPIVN KKEEIVGVAT FYNRKDGKPF DEQDEVLMES 430 440 450 460 470 480 LTQFLGWSVL NTDTYDKMNK LENRKDIAQD MVLYHVRCDK DEIQLILPTR ERLGKEPADC 490 500 510 520 530 540 EEDELGILLK EVLPGPSKFD IYEFHFSDLE CTELELVKCG IQMYYELGVV RKFQIPQEVL 550 560 570 580 590 600 VRFLFSVSKG YRRITYHNWR HGFNVAQTMF TLLTTGKLKS YYTDLEAFAM VTAGLCHDID 610 620 630 640 650 660 HRGTNNLYQM KSQNPLAKLH GSSILERHHL EFGKFLLSEE TLNIYQNLNR RQHEHVIHLM 670 680 690 700 710 720 DIAIIATDLA LYFKKRTMFQ KIVDESKNYE DRKSWVEYLS LETTRKEIVM AMMMTACDLS 730 740 750 760 770 780 AITKPWEVQS KVALLVAAEF WEQGDLERTV LDQQPIPMMD RNKAAELPKL QVGFIDFVCT 790 800 810 820 830 840 FVYKEFSRFH EEILPMFDRL QNNRKEWKAL ADEYEAKLKA LEEEKQQQED RTTAKKAGTE 850 ICNGGPAPKS STCCIL

References

[1]

"Irish setter dogs affected with rod/cone dysplasia contain a nonsense mutation in the rod cGMP phosphodiesterase beta-subunit gene." Suber M.L., Pittler S.J., Qin N., Wright G.C., Holcombe V., Lee R.H., Craft C.M., Lolley R.N., Baehr W.B., Hurwitz R.L. Proc. Natl. Acad. Sci. U.S.A. 90:3968-3972(1993) [PubMed: 8387203] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Red setter. Tissue: Retina.

[2]

Clements P.J. Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Boxer X Doberman. Tissue: Retina.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z23014 mRNA. Translation: CAA80557.1. L13262 mRNA. Translation: AAA30882.1. Sequence problems.
PIR	A47451.
RefSeq	NP_001002934.1.
UniGene	Cfa.3793
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P33726.
Genome annotation databases
Ensembl	ENSCAFT00000036012; ENSCAFP00000031337; ENSCAFG00000016782; Canis familiaris. [Genome view]
GeneID	399653.
KEGG	cfa:399653.
Organism-specific databases
CTD	399653.
Phylogenomic databases
HOVERGEN	P33726.
Enzyme and pathway databases
BRENDA	3.1.4.35. 463.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6B_CANFA

Accession

Primary (citable) accession number: P33726

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	October 13, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)