P33724 (CAV1_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Caveolin-1

Alternative name(s):
Vesicular integral-membrane protein VIP21

Gene names

Name:	CAV1
Synonyms:	CAV

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
Subunit structure	Homooligomer. Interacts with BMX, BTK, GLIPR2, NOSTRIN, SNAP25 and syntaxin By similarity. Interacts (via the N-terminus) with DPP4; the interaction is direct By similarity. Interacts with CTNNB1, CDH1 and JUP. Ref.5 Ref.8
Subcellular location	Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane › caveola; Peripheral membrane protein. Membrane raft By similarity. Note: Colocalized with DPP4 in membrane rafts By similarity. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Ref.4 Ref.8
Post-translational modification	Phosphorylation of isoform Beta on serine residues is constitutive. Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress By similarity.
Sequence similarities	Belongs to the caveolin family.

Ontologies

Keywords
Cellular component	Cell membrane Golgi apparatus Membrane
Coding sequence diversity	Alternative initiation
PTM	Acetylation Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade Inferred from electronic annotation. Source: Compara T cell costimulation Inferred from sequence or structural similarity. Source: UniProtKB angiogenesis Inferred from electronic annotation. Source: Compara calcium ion transport Inferred from electronic annotation. Source: Compara caveola assembly Inferred from electronic annotation. Source: Compara caveolin-mediated endocytosis Inferred from electronic annotation. Source: Compara cellular response to hyperoxia Inferred from electronic annotation. Source: Compara cellular response to starvation Inferred from electronic annotation. Source: Compara cholesterol homeostasis Inferred from electronic annotation. Source: Compara cytosolic calcium ion homeostasis Inferred from electronic annotation. Source: Compara inactivation of MAPK activity Inferred from electronic annotation. Source: Compara lactation Inferred from electronic annotation. Source: Compara lipid storage Inferred from electronic annotation. Source: Compara mammary gland involution Inferred from electronic annotation. Source: Compara membrane depolarization Inferred from electronic annotation. Source: Compara negative regulation of BMP signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of anoikis Inferred from electronic annotation. Source: Compara negative regulation of canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of cytokine-mediated signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of endothelial cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of epithelial cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of nitric oxide biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of nitric-oxide synthase activity Inferred from electronic annotation. Source: Compara negative regulation of peptidyl-serine phosphorylation Inferred from electronic annotation. Source: Compara negative regulation of protein binding Inferred from electronic annotation. Source: Compara negative regulation of protein ubiquitination Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara negative regulation of tyrosine phosphorylation of Stat5 protein Inferred from electronic annotation. Source: Compara nitric oxide homeostasis Inferred from electronic annotation. Source: Compara positive regulation of calcium ion transport into cytosol Inferred from electronic annotation. Source: Compara positive regulation of canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of extrinsic apoptotic signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of intrinsic apoptotic signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of metalloenzyme activity Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-serine phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of vasoconstriction Inferred from electronic annotation. Source: Compara protein homooligomerization Inferred from electronic annotation. Source: Compara protein localization Inferred from electronic annotation. Source: Compara receptor internalization involved in canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara regulation of blood coagulation Inferred from electronic annotation. Source: Compara regulation of fatty acid metabolic process Inferred from electronic annotation. Source: Compara regulation of smooth muscle contraction Inferred from electronic annotation. Source: Compara regulation of the force of heart contraction by chemical signal Inferred from electronic annotation. Source: Compara response to calcium ion Inferred from electronic annotation. Source: Compara response to estrogen stimulus Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara response to ischemia Inferred from electronic annotation. Source: Compara response to progesterone stimulus Inferred from electronic annotation. Source: Compara skeletal muscle tissue development Inferred from electronic annotation. Source: Compara triglyceride metabolic process Inferred from electronic annotation. Source: Compara vasculogenesis Inferred from electronic annotation. Source: Compara vasoconstriction Inferred from electronic annotation. Source: Compara vesicle organization Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell acrosomal membrane Inferred from electronic annotation. Source: Compara apical plasma membrane Inferred from electronic annotation. Source: Compara basolateral plasma membrane Inferred from electronic annotation. Source: Compara caveola Inferred from direct assay Ref.4. Source: UniProtKB cell cortex Inferred from electronic annotation. Source: Compara endoplasmic reticulum Inferred from electronic annotation. Source: Compara endosome Inferred from sequence or structural similarity. Source: UniProtKB integral to plasma membrane Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara protein complex Inferred from electronic annotation. Source: Compara
Molecular_function	peptidase activator activity Inferred from electronic annotation. Source: Compara structural molecule activity Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CTNNB1	P35222	5	EBI-79998,EBI-491549	From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Alpha (identifier: P33724-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: P33724-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 178	177	Caveolin-1		PRO_0000004762
Regions
	Topological domain	2 – 104	103	Cytoplasmic Potential
	Intramembrane	105 – 125	21	Helical; Potential
	Topological domain	126 – 178	53	Cytoplasmic Potential
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	5	1	N6-acetyllysine By similarity
	Modified residue	6	1	Phosphotyrosine By similarity
	Modified residue	14	1	Phosphotyrosine; by ABL1 By similarity
	Modified residue	25	1	Phosphotyrosine By similarity
	Modified residue	37	1	Phosphoserine By similarity
	Modified residue	42	1	Phosphotyrosine By similarity
	Lipidation	133	1	S-palmitoyl cysteine Ref.6
	Lipidation	143	1	S-palmitoyl cysteine Ref.6
	Lipidation	156	1	S-palmitoyl cysteine Ref.6
Natural variations
	Alternative sequence	1 – 31	31	Missing in isoform Beta.		VSP_018691

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha [UniParc]. Last modified February 1, 1994. Version 1. Checksum: DD77CE4D436E508D	FASTA	178	20,606
	10 20 30 40 50 60 MSGGKYVDSE GHLYTVPIRE QGNIYKPNNK AMAEEMSEKQ VYDAHTKEID LVNRDPKHLN 70 80 90 100 110 120 DDVVKIDFED VIAEPEGTHS FDGIWKASFT TFTVTKYWFY RLLSALFGIP MALIWGIYFA 130 140 150 160 170 ILSFLHIWAV VPCIKSFLIE IQCISRVYSI YVHTFCDPFF EAVGKIFSNI RINMQKET
	Isoform Beta [UniParc]. Checksum: E32E90B50FFC5B1F	FASTA	147	17,157
	10 20 30 40 50 60 MAEEMSEKQV YDAHTKEIDL VNRDPKHLND DVVKIDFEDV IAEPEGTHSF DGIWKASFTT 70 80 90 100 110 120 FTVTKYWFYR LLSALFGIPM ALIWGIYFAI LSFLHIWAVV PCIKSFLIEI QCISRVYSIY 130 140 VHTFCDPFFE AVGKIFSNIR INMQKET

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"VIP21, a 21-kD membrane protein is an integral component of trans-Golgi-network-derived transport vesicles." Kurzchalia T.V., Dupree P., Parton R.G., Kellner R., Virta H., Lehnert M., Simons K. J. Cell Biol. 118:1003-1014(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 48-74 AND 166-176. Tissue: Epithelium.
[2]	"Signal transducing molecules and glycosyl-phosphatidylinositol-linked proteins form a caveolin-rich insoluble complex in MDCK cells." Sargiacomo M., Sudol M., Tang Z., Lisanti M.P. J. Cell Biol. 122:789-807(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Cocker spaniel. Tissue: Kidney.
[3]	"Comparative analyses of multi-species sequences from targeted genomic regions." Thomas J.W., Touchman J.W., Blakesley R.W., Bouffard G.G., Beckstrom-Sternberg S.M., Margulies E.H., Blanchette M., Siepel A.C., Thomas P.J., McDowell J.C., Maskeri B., Hansen N.F., Schwartz M.S., Weber R.J., Kent W.J., Karolchik D., Bruen T.C., Bevan R. , Cutler D.J., Schwartz S., Elnitski L., Idol J.R., Prasad A.B., Lee-Lin S.-Q., Maduro V.V.B., Summers T.J., Portnoy M.E., Dietrich N.L., Akhter N., Ayele K., Benjamin B., Cariaga K., Brinkley C.P., Brooks S.Y., Granite S., Guan X., Gupta J., Haghighi P., Ho S.-L., Huang M.C., Karlins E., Laric P.L., Legaspi R., Lim M.J., Maduro Q.L., Masiello C.A., Mastrian S.D., McCloskey J.C., Pearson R., Stantripop S., Tiongson E.E., Tran J.T., Tsurgeon C., Vogt J.L., Walker M.A., Wetherby K.D., Wiggins L.S., Young A.C., Zhang L.-H., Osoegawa K., Zhu B., Zhao B., Shu C.L., De Jong P.J., Lawrence C.E., Smit A.F., Chakravarti A., Haussler D., Green P., Miller W., Green E.D. Nature 424:788-793(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Caveolae and sorting in the trans-Golgi network of epithelial cells." Dupree P., Parton R.G., Raposo G., Kurzchalia T.V., Simons K. EMBO J. 12:1597-1605(1993) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Oligomeric structure of caveolin: implications for caveolae membrane organization." Sargiacomo M., Scherer P.E., Tang Z., Kubler E., Song K.S., Sanders M.C., Lisanti M.P. Proc. Natl. Acad. Sci. U.S.A. 92:9407-9411(1995) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
[6]	"Caveolin is palmitoylated on multiple cysteine residues. Palmitoylation is not necessary for localization of caveolin to caveolae." Dietzen D.J., Hastings W.R., Lublin D.M. J. Biol. Chem. 270:6838-6842(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PALMITOYLATION AT CYS-133; CYS-143 AND CYS-156.
[7]	"Caveolin isoforms differ in their N-terminal protein sequence and subcellular distribution. Identification and epitope mapping of an isoform-specific monoclonal antibody probe." Scherer P.E., Tang Z., Chun M., Sargiacomo M., Lodish H.F., Lisanti M.P. J. Biol. Chem. 270:16395-16401(1995) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE INITIATION.
[8]	"Caveolin-1 expression inhibits Wnt/beta-catenin/Lef-1 signaling by recruiting beta-catenin to caveolae membrane domains." Galbiati F., Volonte D., Brown A.M., Weinstein D.E., Ben-Ze'ev A., Pestell R.G., Lisanti M.P. J. Biol. Chem. 275:23368-23377(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CTNNB1; CDH1 AND JUP, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z12161 Genomic DNA. Translation: CAA78151.1. U47060 mRNA. Translation: AAA87050.1. DP000236 Genomic DNA. Translation: AAR16266.1.
PIR	A43419.
RefSeq	NP_001003296.1. NM_001003296.1.
UniGene	Cfa.3850.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P33724. 5 interactions.
Proteomic databases
PaxDb	P33724.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCAFT00000005462; ENSCAFP00000005060; ENSCAFG00000003404.
GeneID	403980.
KEGG	cfa:403980.
Organism-specific databases
CTD	857.
Phylogenomic databases
eggNOG	NOG86001.
GeneTree	ENSGT00390000014924.
HOGENOM	HOG000036550.
HOVERGEN	HBG003422.
InParanoid	P33724.
KO	K06278.
OMA	VHTFCDP.
OrthoDB	EOG4JQ3ZP.
Family and domain databases
InterPro	IPR001612. Caveolin. IPR015504. Caveolin_1. IPR018361. Caveolin_CS. [Graphical view]
PANTHER	PTHR10844. PTHR10844. 1 hit. PTHR10844:SF5. PTHR10844:SF5. 1 hit.
Pfam	PF01146. Caveolin. 1 hit. [Graphical view]
PROSITE	PS01210. CAVEOLIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20817470.

Entry information

Entry name

CAV1_CANFA

Accession

Primary (citable) accession number: P33724
Secondary accession number(s): A0M8U8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program