P33712 (IGF1_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor I
Short name=IGF-I

Alternative name(s):
Somatomedin

Gene names

Name:	IGF1
Synonyms:	IGFIA

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake By similarity.
Subcellular location	Secreted.
Polymorphism	The domestic dog exhibits greater diversity in body size than any other terrestrial vertebrate. A major quantitative trait locus (QTL) on chromosome 15 influences size variation within a single breed. In particular, a single-nucleotide polymorphism haplotype in IGF1 (synonymous SNP in exon 3) is common to all small breeds (less than 9 kg) and nearly absent from giant breeds (more than 30 kg), suggesting that the same causal sequence variant is a major contributor to body size in all small dogs.
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of release of cytochrome c from mitochondria Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of glucose import Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of glycogen biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	extracellular space Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Propeptide	? – 48		By similarity		PRO_0000330685
	Chain	49 – 118	70	Insulin-like growth factor I		PRO_0000015650
	Propeptide	119 – 153	35	E peptide		PRO_0000015651
Regions
	Region	49 – 77	29	B
	Region	78 – 89	12	C
	Region	90 – 110	21	A
	Region	111 – 118	8	D
Amino acid modifications
	Disulfide bond	54 ↔ 96		By similarity
	Disulfide bond	66 ↔ 109		By similarity
	Disulfide bond	95 ↔ 100		By similarity
Experimental info
	Sequence conflict	150 – 153	4	NYRM → TY in L08254. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P33712 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 19D16DBF9151C0EF	FASTA	153	16,970
	10 20 30 40 50 60 MGKISSLPTQ LFKCCFCDFL KVKMHTVSSS HLFYLALCLL TFPSPATAGP ETLCGAELVD 70 80 90 100 110 120 ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSARS 130 140 150 VRAQRHTDMP KAQKEVHLKN ASRGSAGNKN YRM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Staten N.R. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Synovium.
[2]	"Sequence of a cDNA encoding dog insulin-like growth factor I." Delafontaine P., Lou H., Harrison D.G., Bernstein K.E. Gene 130:305-306(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-151.
[3]	"A single IGF1 allele is a major determinant of small size in dogs." Sutter N.B., Bustamante C.D., Chase K., Gray M.M., Zhao K., Zhu L., Padhukasahasram B., Karlins E., Davis S., Jones P.G., Quignon P., Johnson G.S., Parker H.G., Fretwell N., Mosher D.S., Lawler D.F., Satyaraj E., Nordborg M. , Lark K.G., Wayne R.K., Ostrander E.A. Science 316:112-115(2007) [PubMed] [Europe PMC] [Abstract] Cited for: POLYMORPHISM.

Web resources

Protein Spotlight

A dog's life - Issue 92 of March 2008

Cross-references

Sequence databases
EMBL GenBank DDBJ	DN380306 mRNA. No translation available. L08254 mRNA. No translation available.
PIR	PN0622.
RefSeq	XP_853117.1. XM_848024.2.
UniGene	Cfa.3888.
3D structure databases
ProteinModelPortal	P33712.
SMR	P33712. Positions 49-118.
ModBase	Search...
Protein-protein interaction databases
STRING	9615.ENSCAFP00000010834.
Proteomic databases
PaxDb	P33712.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCAFT00000011690; ENSCAFP00000010833; ENSCAFG00000007304.
GeneID	610255.
KEGG	cfa:610255.
Organism-specific databases
CTD	3479.
Phylogenomic databases
eggNOG	NOG39886.
GeneTree	ENSGT00530000063856.
HOGENOM	HOG000233362.
HOVERGEN	HBG006137.
KO	K05459.
OMA	HTVSYIH.
OrthoDB	EOG4NGGNT.
Family and domain databases
Gene3D	1.10.100.10. 1 hit.
InterPro	IPR022341. IGF-I. IPR016179. Insulin-like. IPR022350. Insulin-like_growth_factor. IPR022353. Insulin_CS. IPR022352. Insulin_family. [Graphical view]
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR02002. INSLNLIKEGF. PR02005. INSLNLIKEGF1. PR00276. INSULINFAMLY.
SMART	SM00078. IlGF. 1 hit. [Graphical view]
SUPFAM	SSF56994. Insulin-like. 1 hit.
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20895820.

Entry information

Entry name

IGF1_CANFA

Accession

Primary (citable) accession number: P33712

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	April 29, 2008
Last modified:	April 3, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program