Reviewed, UniProtKB/Swiss-Prot P33712 (IGF1_CANFA)

Names and origin

Protein names

Recommended name:
    Insulin-like growth factor I
      Short name=IGF-I
Alternative name(s):
    Somatomedin

Gene names

Name:	IGF1
Synonyms:	IGFIA

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.
Subcellular location	Secreted.
Polymorphism	The domestic dog exhibits greater diversity in body size than any other terrestrial vertebrate. A major quantitative trait locus (QTL) on chromosome 15 influences size variation within a single breed. In particular, a single-nucleotide polymorphism haplotype in IGF1 (synonymous SNP in exon 3) is common to all small breeds (less than 9 kg) and nearly absent from giant breeds (more than 30 kg), suggesting that the same causal sequence variant is a major contributor to body size in all small dogs.
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – ?		Potential
	Propeptide	? – 48		By similarity
	Chain	49 – 118	70	Insulin-like growth factor I
	Propeptide	119 – 153	35	E peptide
Regions
	Region	49 – 77	29	B
	Region	78 – 89	12	C
	Region	90 – 110	21	A
	Region	111 – 118	8	D
Amino acid modifications
	Disulfide bond	54 ↔ 96		By similarity
	Disulfide bond	66 ↔ 109		By similarity
	Disulfide bond	95 ↔ 100		By similarity
Experimental info
	Sequence conflict	150 – 153	4	NYRM → TY in L08254. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P33712-1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 19D16DBF9151C0EF	FASTA	153	16,970
	10 20 30 40 50 60 MGKISSLPTQ LFKCCFCDFL KVKMHTVSSS HLFYLALCLL TFPSPATAGP ETLCGAELVD 70 80 90 100 110 120 ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSARS 130 140 150 VRAQRHTDMP KAQKEVHLKN ASRGSAGNKN YRM

References

[1]	Staten N.R. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Synovium.
[2]	"Sequence of a cDNA encoding dog insulin-like growth factor I." Delafontaine P., Lou H., Harrison D.G., Bernstein K.E. Gene 130:305-306(1993) [PubMed: 8359700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-151.
[3]	"A single IGF1 allele is a major determinant of small size in dogs." Sutter N.B., Bustamante C.D., Chase K., Gray M.M., Zhao K., Zhu L., Padhukasahasram B., Karlins E., Davis S., Jones P.G., Quignon P., Johnson G.S., Parker H.G., Fretwell N., Mosher D.S., Lawler D.F., Satyaraj E., Nordborg M. , Lark K.G., Wayne R.K., Ostrander E.A. Science 316:112-115(2007) [PubMed: 17412960] [Abstract] Cited for: POLYMORPHISM.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

A dog's life - Issue 92 of March 2008

Cross-references

Sequence databases
EMBLGenBankDDBJ	DN380306 mRNA. No translation available. L08254 mRNA. No translation available.
PIR	PN0622.
RefSeq	XP_853117.1.
3D structure databases
HSSP	HSSP built from PDB template 2GF1 based on UniProtKB P01343.
SMR	P33712. Positions 20-89.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCAFG00000007304. Canis familiaris. [Contig view]
GeneID	610255.
KEGG	cfa:610255.
Phylogenomic databases
HOVERGEN	P33712.
Family and domain databases
InterPro	IPR004825. Ins/IGF/relaxin. [Graphical view]
Gene3D	G3DSA:1.10.100.10. Ins/IGF/relaxin. 1 hit.
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR00276. INSULINA. PR00277. INSULINB.
ProDom	PD001048. Bombyxin. 1 hit. PD015667. Mollusc_ins. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00078. IlGF. 1 hit. [Graphical view]
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

IGF1_CANFA

Accession

Primary (citable) accession number: P33712

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	April 29, 2008
Last modified:	September 2, 2008
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)