Reviewed, UniProtKB/Swiss-Prot P33712 (IGF1_CANFA)

Names and origin

Protein names

Recommended name:
    Insulin-like growth factor I
      Short name=IGF-I
Alternative name(s):
    Somatomedin

Gene names

Name:	IGF1
Synonyms:	IGFIA

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.
Subcellular location	Secreted.
Polymorphism	The domestic dog exhibits greater diversity in body size than any other terrestrial vertebrate. A major quantitative trait locus (QTL) on chromosome 15 influences size variation within a single breed. In particular, a single-nucleotide polymorphism haplotype in IGF1 (synonymous SNP in exon 3) is common to all small breeds (less than 9 kg) and nearly absent from giant breeds (more than 30 kg), suggesting that the same causal sequence variant is a major contributor to body size in all small dogs.
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor
PTM	Disulfide bond
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW hormone activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Propeptide	? – 48		By similarity		PRO_0000330685
	Chain	49 – 118	70	Insulin-like growth factor I		PRO_0000015650
	Propeptide	119 – 153	35	E peptide		PRO_0000015651
Regions
	Region	49 – 77	29	B
	Region	78 – 89	12	C
	Region	90 – 110	21	A
	Region	111 – 118	8	D
Amino acid modifications
	Disulfide bond	54 ↔ 96		By similarity
	Disulfide bond	66 ↔ 109		By similarity
	Disulfide bond	95 ↔ 100		By similarity
Experimental info
	Sequence conflict	150 – 153	4	NYRM → TY in L08254. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P33712-1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 19D16DBF9151C0EF	FASTA	153	16,970
	10 20 30 40 50 60 MGKISSLPTQ LFKCCFCDFL KVKMHTVSSS HLFYLALCLL TFPSPATAGP ETLCGAELVD 70 80 90 100 110 120 ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSARS 130 140 150 VRAQRHTDMP KAQKEVHLKN ASRGSAGNKN YRM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Staten N.R. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Synovium.
[2]	"Sequence of a cDNA encoding dog insulin-like growth factor I." Delafontaine P., Lou H., Harrison D.G., Bernstein K.E. Gene 130:305-306(1993) [PubMed: 8359700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-151.
[3]	"A single IGF1 allele is a major determinant of small size in dogs." Sutter N.B., Bustamante C.D., Chase K., Gray M.M., Zhao K., Zhu L., Padhukasahasram B., Karlins E., Davis S., Jones P.G., Quignon P., Johnson G.S., Parker H.G., Fretwell N., Mosher D.S., Lawler D.F., Satyaraj E., Nordborg M. , Lark K.G., Wayne R.K., Ostrander E.A. Science 316:112-115(2007) [PubMed: 17412960] [Abstract] Cited for: POLYMORPHISM.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

A dog's life - Issue 92 of March 2008

Cross-references

Sequence databases
EMBL GenBank DDBJ	DN380306 mRNA. No translation available. L08254 mRNA. No translation available.
PIR	PN0622.
RefSeq	XP_853117.1.
UniGene	Cfa.3888
3D structure databases
SMR	P33712. Positions 49-118.
ModBase	Search...
Protein-protein interaction databases
STRING	P33712.
Genome annotation databases
Ensembl	ENSCAFT00000011690; ENSCAFP00000010833; ENSCAFG00000007304; Canis familiaris. [Genome view]
GeneID	610255.
KEGG	cfa:610255.
Organism-specific databases
CTD	610255.
Phylogenomic databases
eggNOG	maNOG19426.
HOVERGEN	P33712.
Family and domain databases
InterPro	IPR004825. Ins/IGF/relaxin. IPR016179. Insulin-like. [Graphical view]
Gene3D	G3DSA:1.10.100.10. Ins/IGF/relaxin. 1 hit.
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR00277. INSULINB.
SMART	SM00078. IlGF. 1 hit. [Graphical view]
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IGF1_CANFA

Accession

Primary (citable) accession number: P33712

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	April 29, 2008
Last modified:	January 19, 2010
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)