Reviewed, UniProtKB/Swiss-Prot P33696 (EXON_RHIME)

Names and origin

Protein names

Recommended name:
    UTP--glucose-1-phosphate uridylyltransferase
    EC=2.7.7.9
Alternative name(s):
    UDP-glucose pyrophosphorylase
      Short name=UDPGP
    Alpha-D-glucosyl-1-phosphate uridylyltransferase
    Uridine diphosphoglucose pyrophosphorylase

Gene names

Name:	exoN
Ordered Locus Names:	RB1085
ORF Names:	SMb20960

Encoded on

Plasmid pSymB (megaplasmid 2)

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.
Pathway	Carbohydrate metabolism; nucleotide-sugar metabolism.
Sequence similarities	Belongs to the UDPGP type 2 family.

Ontologies

Keywords
Biological process	Exopolysaccharide synthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	polysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	UTP--glucose-1-phosphate uridylyltransferase		PRO_0000201347
Experimental info
	Sequence conflict	275 – 301	27	ARADI…RIRAA → LVRTSAISFSNPSATWFCLT KAASGRHKRRLPAVLRDCRN KDRRRR Ref.1
	Sequence conflict	275 – 301	27	ARADI…RIRAA → LVRTSAISFSNPSATWFCLT KAASGRHKRRLPAVLRDCRN KDRRRR Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P33696-1 [UniParc]. Last modified October 24, 2001. Version 2. Checksum: 03AE86FB658E9532	FASTA	301	32,584
	10 20 30 40 50 60 MDRVRTVRKA VIPVAGNGTR FLPATKAVPK EMLTIVDRPV VQYAVDEARQ AGIEHIVFVT 70 80 90 100 110 120 SRNKQVIEDH FDDAPELISS LSRSGKSAQI SELEAMLPAA GSVSFTRQQA PLGLGHAVWC 130 140 150 160 170 180 ARDLIGDEPF ALLLPDMVSF GARGCVAGLM DLYHEVGGNV VGVEQCAPEE ASKYGIVGKG 190 200 210 220 230 240 ETVRHGFSVT EMVEKPAAGK APSNYYLNGR YILQPEIFSI LAHQTRGAGN EIQLTDGMLT 250 260 270 280 290 300 LSQSQSFHAH PYEGRTFDCG SKQGFIEANV AFALARADIG DIVFESVRDM VLSHESRIRA A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Family of glycosyl transferases needed for the synthesis of succinoglycan by Rhizobium meliloti." Glucksmann M.A., Reuber T.L., Walker G.C. J. Bacteriol. 175:7033-7044(1993) [PubMed: 8226645] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1021.
[2]	"Genes needed for the modification, polymerization, export, and processing of succinoglycan by Rhizobium meliloti: a model for succinoglycan biosynthesis." Glucksmann M.A., Reuber T.L., Walker G.C. J. Bacteriol. 175:7045-7055(1993) [PubMed: 8226646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1021.
[3]	"Identification and analysis of the Rhizobium meliloti exoAMONP genes involved in exopolysaccharide biosynthesis and mapping of promoters located on the exoHKLAMONP fragment." Becker A., Kleickmann A., Keller M., Arnold W., Puehler A. Mol. Gen. Genet. 241:367-379(1993) [PubMed: 8246891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RCR2011 / SU47.
[4]	"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti." Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A. Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed: 11481431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L20758 Unassigned DNA. Translation: AAA16043.1. Z22636 Genomic DNA. Translation: CAA80348.1. AL591985 Genomic DNA. Translation: CAC49485.1.
PIR	D49349. E95977.
RefSeq	NP_437625.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1237416.
GenomeReviews	Gene locus RB1085 in contig AL591985_GR.
KEGG	sme:SM_b20960.
NMPDR	fig|266834.1.peg.5720.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P33696.
OMA	ELENTEP.
Enzyme and pathway databases
BioCyc	SMEL266834:SMB20960-MON.
BRENDA	2.7.7.9. 142.
Family and domain databases
InterPro	IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EXON_RHIME

Accession

Primary (citable) accession number: P33696

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	October 24, 2001
Last modified:	November 3, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)