Reviewed, UniProtKB/Swiss-Prot P33682 (GUN1_STRHA)

Names and origin

Protein names

Recommended name:
    Endoglucanase 1
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta-glucanase 1
    Cellulase I
    CEL1
    CMCase I

Gene names

Name:

celA1

Organism

Streptomyces halstedii

Taxonomic identifier

1944 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Implicated in the mechanism of induction exerted by cellobiose.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 6 (cellulase B) family.
Caution	It is uncertain whether Met-1 or Met-9 is the initiator.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.1
	Chain	28 – 321	294	Endoglucanase 1		PRO_0000007906
Sites
	Active site	110	1	By similarity
	Active site	149	1	Proton donor By similarity
	Active site	295	1	Nucleophile By similarity
Amino acid modifications
	Disulfide bond	112 ↔ 156		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33682-1 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: DC5E39EF3CCD114C	FASTA	321	33,695
	10 20 30 40 50 60 MSRKLRTLMA ALCALPLAFA AAPPAHAADP TTMTNGFYAD PDSSASRWAA ANPGDGRAAA 70 80 90 100 110 120 INASIANTPM ARWFGSWSGA IGTAAGAYAG AADGRDKLPI LVAYNIYNRD YCGGHSAGGA 130 140 150 160 170 180 ASPSAYADWI ARFAGGIAAR PAVVILEPDS LGDYGCMNPA QIDEREAMLT NALVQFNRQA 190 200 210 220 230 240 PNTWVYMDAG NPRWADAATM ARRLHEAGLR QAHGFSLNVS NYITTAENTA YGNAVNNELA 250 260 270 280 290 300 ARYGYTKPFV VDTSRNGNGS NGEWCNPSGR RIGTPTRTGG GAEMLLWIKT PGESDGNCGV 310 320 GSGSTAGQFL PEVAYKMIYG Y

References

[1]

"Cloning and nucleotide sequence of celA1, and endo-beta-1,4-glucanase-encoding gene from Streptomyces halstedii JM8." Fernandez-Abalos J.M., Sanchez P., Coll-Fresno P.M., Villanueva J.R., Perez P., Santamaria R.I. J. Bacteriol. 174:6368-6376(1992) [PubMed: 1400190] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-33. Strain: JM8 / CECT3310.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z12157 Genomic DNA. Translation: CAA78145.1.
3D structure databases
HSSP	HSSP built from PDB template 1TML based on UniProtKB P26222.
ModBase	Search...
Protein family/group databases
CAZy	GH6. Glycoside Hydrolase Family 6.
Enzyme and pathway databases
BRENDA	3.2.1.4. 119240.
Family and domain databases
InterPro	IPR016288. Beta_cellobiohydrolase. IPR001524. Glyco_hydro_6_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.40. Glyco_hydro_6. 1 hit.
Pfam	PF01341. Glyco_hydro_6. 1 hit. [Graphical view]
PIRSF	PIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTS	PR00733. GLHYDRLASE6.
ProDom	PD003733. Glyco_hydro_6. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00655. GLYCOSYL_HYDROL_F6_1. False negative. PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN1_STRHA

Accession

Primary (citable) accession number: P33682

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)