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P33682 (GUN1_STRHA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 1

EC=3.2.1.4
Alternative name(s):
CEL1
CMCase I
Cellulase I
Endo-1,4-beta-glucanase 1
Gene names
Name:celA1
OrganismStreptomyces halstedii
Taxonomic identifier1944 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in the mechanism of induction exerted by cellobiose.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 6 (cellulase B) family.

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.1
Chain28 – 321294Endoglucanase 1
PRO_0000007906

Sites

Active site1101 By similarity
Active site1491Proton donor By similarity
Active site2951Nucleophile By similarity

Amino acid modifications

Disulfide bond112 ↔ 156 By similarity

Sequences

Sequence LengthMass (Da)Tools
P33682 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: DC5E39EF3CCD114C

FASTA32133,695
        10         20         30         40         50         60 
MSRKLRTLMA ALCALPLAFA AAPPAHAADP TTMTNGFYAD PDSSASRWAA ANPGDGRAAA 

        70         80         90        100        110        120 
INASIANTPM ARWFGSWSGA IGTAAGAYAG AADGRDKLPI LVAYNIYNRD YCGGHSAGGA 

       130        140        150        160        170        180 
ASPSAYADWI ARFAGGIAAR PAVVILEPDS LGDYGCMNPA QIDEREAMLT NALVQFNRQA 

       190        200        210        220        230        240 
PNTWVYMDAG NPRWADAATM ARRLHEAGLR QAHGFSLNVS NYITTAENTA YGNAVNNELA 

       250        260        270        280        290        300 
ARYGYTKPFV VDTSRNGNGS NGEWCNPSGR RIGTPTRTGG GAEMLLWIKT PGESDGNCGV 

       310        320 
GSGSTAGQFL PEVAYKMIYG Y 

« Hide

References

[1]"Cloning and nucleotide sequence of celA1, and endo-beta-1,4-glucanase-encoding gene from Streptomyces halstedii JM8."
Fernandez-Abalos J.M., Sanchez P., Coll-Fresno P.M., Villanueva J.R., Perez P., Santamaria R.I.
J. Bacteriol. 174:6368-6376(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-33.
Strain: JM8 / CECT3310.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z12157 Genomic DNA. Translation: CAA78145.1.

3D structure databases

ProteinModelPortalP33682.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH6. Glycoside Hydrolase Family 6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.40. 1 hit.
InterProIPR016288. Beta_cellobiohydrolase.
IPR001524. Glyco_hydro_6_CS.
[Graphical view]
PfamPF01341. Glyco_hydro_6. 1 hit.
[Graphical view]
PIRSFPIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTSPR00733. GLHYDRLASE6.
SUPFAMSSF51989. SSF51989. 1 hit.
PROSITEPS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN1_STRHA
AccessionPrimary (citable) accession number: P33682
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 1, 1994
Last modified: February 19, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries