P33681 (CD80_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
T-lymphocyte activation antigen CD80

Alternative name(s):
Activation B7-1 antigen
BB1
CTLA-4 counter-receptor B7.1
Short name=B7
CD_antigen=CD80

Gene names

Name:	CD80
Synonyms:	CD28LG, CD28LG1, LAB7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
Subunit structure	Interacts with adenovirus subgroup B fiber proteins and acts as a receptor for these viruses. Ref.6
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed on activated B-cells, macrophages and dendritic cells.
Sequence similarities	Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Host-virus interaction
Cellular component	Membrane
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	T cell activation Inferred by curator PubMed 9915850. Source: UniProtKB T cell costimulation Traceable author statement. Source: Reactome cell-cell signaling Inferred by curator PubMed 9915850. Source: UniProtKB intracellular signal transduction Non-traceable author statement PubMed 9915850. Source: UniProtKB positive regulation of T-helper 1 cell differentiation Non-traceable author statement PubMed 9915850. Source: UniProtKB positive regulation of alpha-beta T cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process Non-traceable author statement PubMed 9915850. Source: UniProtKB positive regulation of interleukin-2 biosynthetic process Non-traceable author statement PubMed 9915850. Source: UniProtKB positive regulation of peptidyl-tyrosine phosphorylation Inferred from direct assay PubMed 9915850. Source: UniProtKB positive regulation of signal transduction Non-traceable author statement PubMed 9915850. Source: UniProtKB positive regulation of transcription, DNA-dependent Non-traceable author statement PubMed 9915850. Source: UniProtKB virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	external side of plasma membrane Inferred from electronic annotation. Source: Compara integral to membrane Inferred by curator PubMed 9915850. Source: UniProtKB plasma membrane Traceable author statement. Source: Reactome
Molecular_function	coreceptor activity Non-traceable author statement PubMed 9915850. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CD274	Q9NZQ7	2	EBI-1031024,EBI-4314282
CTLA4	P16410	3	EBI-1031024,EBI-1030991
NGFR	P08138	3	EBI-1031024,EBI-1387782

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 34

34

Ref.4

Chain

35 – 288

254

T-lymphocyte activation antigen CD80

PRO_0000014547

Regions

Topological domain

35 – 242

208

Extracellular Potential

Transmembrane

243 – 263

21

Helical; Potential

Topological domain

264 – 288

25

Cytoplasmic Potential

Domain

35 – 135

101

Ig-like V-type

Domain

145 – 230

86

Ig-like C2-type

Amino acid modifications

Glycosylation

53

1

N-linked (GlcNAc...) Potential

Glycosylation

89

1

N-linked (GlcNAc...) Potential

Glycosylation

98

1

N-linked (GlcNAc...) Potential

Glycosylation

186

1

N-linked (GlcNAc...)

Glycosylation

207

1

N-linked (GlcNAc...)

Glycosylation

211

1

N-linked (GlcNAc...) Potential

Glycosylation

226

1

N-linked (GlcNAc...)

Glycosylation

232

1

N-linked (GlcNAc...) Potential

Disulfide bond

50 ↔ 116

Disulfide bond

162 ↔ 216

Secondary structure

1

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288

Helix Strand Turn

Details...

Beta strand

37 – 41

5

Beta strand

46 – 48

3

Helix

58 – 61

4

Beta strand

63 – 68

6

Beta strand

71 – 77

7

Beta strand

80 – 83

4

Helix

85 – 88

4

Beta strand

91 – 95

5

Turn

96 – 99

4

Beta strand

100 – 103

4

Helix

108 – 110

3

Beta strand

112 – 120

9

Beta strand

127 – 139

13

Beta strand

146 – 151

6

Beta strand

157 – 169

13

Beta strand

171 – 179

9

Beta strand

185 – 191

7

Turn

193 – 195

3

Beta strand

198 – 207

10

Beta strand

212 – 220

9

Beta strand

225 – 231

7

Sequences

	Sequence		Length	Mass (Da)
	P33681 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: BA453EE34528B1F4	FASTA	288	33,048
	10 20 30 40 50 60 MGHTRRQGTS PSKCPYLNFF QLLVLAGLSH FCSGVIHVTK EVKEVATLSC GHNVSVEELA 70 80 90 100 110 120 QTRIYWQKEK KMVLTMMSGD MNIWPEYKNR TIFDITNNLS IVILALRPSD EGTYECVVLK 130 140 150 160 170 180 YEKDAFKREH LAEVTLSVKA DFPTPSISDF EIPTSNIRRI ICSTSGGFPE PHLSWLENGE 190 200 210 220 230 240 ELNAINTTVS QDPETELYAV SSKLDFNMTT NHSFMCLIKY GHLRVNQTFN WNTTKQEHFP 250 260 270 280 DNLLPSWAIT LISVNGIFVI CCLTYCFAPR CRERRRNERL RRESVRPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells." Freeman G.J., Freedman A.S., Segil J.M., Lee G., Whitman J.F., Nadler L.M. J. Immunol. 143:2714-2722(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lymphoid tissue.
[2]	"Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7." Selvakumar A., Mohanraj B.K., Eddy R.L., Shows T.B., White P.C., Dupont B. Immunogenetics 36:175-181(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7." Freeman G.J., Gray G.S., Gimmi C.D., Lombard D.B., Zhou L.-J., White M., Fingeroth J.D., Gribben J.G., Nadler L.M. J. Exp. Med. 174:625-631(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 35-38.
[5]	"CD80 (B7) and CD86 (B70) provide similar costimulatory signals for T cell proliferation, cytokine production, and generation of CTL." Lanier L.L., O'Fallon S., Somoza C., Phillips J.H., Linsley P.S., Okumura K., Ito D., Azuma M. J. Immunol. 154:97-105(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Members of adenovirus species B utilize CD80 and CD86 as cellular attachment receptors." Short J.J., Vasu C., Holterman M.J., Curiel D.T., Pereboev A. Virus Res. 122:144-153(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ADENOVIRUS SUBGROUP B FIBER PROTEINS.
[7]	"Structure and dimerization of a soluble form of B7-1." Ikemizu S., Gilbert R.J., Fennelly J.A., Collins A.V., Harlos K., Jones E.Y., Stuart D.I., Davis S.J. Immunity 12:51-60(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 35-234.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M27533 mRNA. Translation: AAA36045.1.
M83077 , M83072, M83073, M83074 Genomic DNA. Translation: AAA58390.1.
BC042665 mRNA. Translation: AAH42665.1.

IPI

IPI00015914.

PIR

A45803. I54495.

RefSeq

NP_005182.1. NM_005191.3.

UniGene

Hs.838.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DR9	X-ray	3.00	A	35-233	[»]
1I8L	X-ray	3.00	A/B	35-242	[»]

ProteinModelPortal

P33681.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6044N.

IntAct

P33681. 4 interactions.

STRING

9606.ENSP00000264246.

PTM databases

PhosphoSite

P33681.

Polymorphism databases

DMDM

461606.

Proteomic databases

PaxDb

P33681.

PRIDE

P33681.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000264246; ENSP00000264246; ENSG00000121594.
ENST00000478182; ENSP00000418364; ENSG00000121594.

GeneID

941.

KEGG

hsa:941.

UCSC

uc003ecq.3. human.

Organism-specific databases

CTD

941.

GeneCards

GC03M119243.

HGNC

HGNC:1700. CD80.

HPA

CAB025368.

MIM

112203. gene.

neXtProt

NX_P33681.

PharmGKB

PA26239.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG42168.

HOGENOM

HOG000036959.

HOVERGEN

HBG000261.

InParanoid

P33681.

KO

K05412.

OMA

VRIYWQK.

OrthoDB

EOG45HRZB.

PhylomeDB

P33681.

Enzyme and pathway databases

Pathway_Interaction_DB

il12_stat4pathway. IL12 signaling mediated by STAT4.
tcrpathway. TCR signaling in naive CD4+ T cells.
cd8tcrpathway. TCR signaling in naive CD8+ T cells.

Reactome

REACT_111102. Signal Transduction.
REACT_116125. Disease.
REACT_6900. Immune System.

Gene expression databases

ArrayExpress

P33681.

Bgee

P33681.

CleanEx

HS_CD80.

Genevestigator

P33681.

GermOnline

ENSG00000121594. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 2 hits.

InterPro

IPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]

Pfam

PF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]

SMART

SM00409. IG. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB01281. Abatacept.

EvolutionaryTrace

P33681.

GenomeRNAi

941.

NextBio

3898.

SOURCE

Search...

Entry information

Entry name

CD80_HUMAN

Accession

Primary (citable) accession number: P33681

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 137 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.