Reviewed, UniProtKB/Swiss-Prot P33677 (FDH_PICAN)

Names and origin

Protein names

Recommended name:
    Formate dehydrogenase
    EC=1.2.1.2
Alternative name(s):
    NAD-dependent formate dehydrogenase
      Short name=FDH

Gene names

Name:

FMDH

Organism

Pichia angusta (Yeast) (Hansenula polymorpha)

Taxonomic identifier

4905 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Formate + NAD+ = CO2 + NADH.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Probable
	Chain	2 – 362	361	Formate dehydrogenase		PRO_0000076025
Sites
	Active site	258	1	By similarity
	Active site	311	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33677-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0037BFD132FC055B	FASTA	362	39,911
	10 20 30 40 50 60 MKVVLVLYDA GKHAQDEERL YGCTENALGI RDWLEKQGHD VVVTSDKEGQ NSVLEKNISD 70 80 90 100 110 120 ADVIISTPFH PAYITKERID KAKKLKLLVV AGVGSDHIDL DYINQSGRDI SVLEVTGSNV 130 140 150 160 170 180 VSVAEHVVMT MLVLVRNFVP AHEQIISGGW NVAEIAKDSF DIEGKVIATI GAGRIGYRVL 190 200 210 220 230 240 ERLVAFNPKE LLYYDYQSLS KEAEEKVGAR RVHDIKELVA QADIVTINCP LHAGSKGLVN 250 260 270 280 290 300 AELLKHFKKG AWLVNTARGA ICVAEDVAAA VKSGQLRGYG GDVWFPQPAP KDHPWRSMAN 310 320 330 340 350 360 KYGAGNAMTP HYSGSVIDAQ VRYAQGTKNI LESFFTQKFD YRPQDIILLN GKYKTKSYGA DK

References

[1]	"DNA-molecules coding for FMDH control regions and structured gene for a protein having FMDH-activity and their uses." Hollenberg C.P., Janowicz Z. Patent number EP0299108, 18-JAN-1989 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	A06214 Unassigned DNA. Translation: CAA00531.1.
3D structure databases
HSSP	HSSP built from PDB template 2NAD based on UniProtKB P33160.
SMR	P33677. Positions 2-353.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.2. 276392.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. [Graphical view]
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDH_PICAN

Accession

Primary (citable) accession number: P33677

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)