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Protein

Chitosanase

Gene

csn

Organism
Bacillus circulans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Aids in the defense against invading fungal pathogens by degrading their cell wall chitosan.

Catalytic activityi

Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei79Proton donorPROSITE-ProRule annotation1
Active sitei97NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17652.
BRENDAi3.2.1.132. 649.

Protein family/group databases

CAZyiGH46. Glycoside Hydrolase Family 46.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitosanase (EC:3.2.1.132)
Gene namesi
Name:csn
OrganismiBacillus circulans
Taxonomic identifieri1397 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

DrugBankiDB01296. Glucosamine.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 42Add BLAST42
ChainiPRO_000001220643 – 301ChitosanaseAdd BLAST259

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi92 ↔ 166

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1301
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi45 – 48Combined sources4
Helixi51 – 61Combined sources11
Helixi65 – 80Combined sources16
Helixi85 – 90Combined sources6
Beta strandi96 – 99Combined sources4
Turni104 – 107Combined sources4
Beta strandi115 – 117Combined sources3
Helixi125 – 130Combined sources6
Helixi138 – 144Combined sources7
Beta strandi149 – 152Combined sources4
Beta strandi155 – 158Combined sources4
Helixi162 – 170Combined sources9
Helixi171 – 174Combined sources4
Helixi176 – 189Combined sources14
Helixi191 – 199Combined sources9
Helixi206 – 219Combined sources14
Beta strandi221 – 224Combined sources4
Helixi228 – 233Combined sources6
Helixi241 – 256Combined sources16
Turni259 – 261Combined sources3
Helixi266 – 279Combined sources14
Helixi289 – 295Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QGIX-ray1.60A43-301[»]
2D05X-ray2.00A43-301[»]
ProteinModelPortaliP33673.
SMRiP33673.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33673.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 46 family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd00978. chitosanase_glyco_hydro_46. 1 hit.
Gene3Di3.30.386.10. 1 hit.
InterProiIPR000400. Glyco_hydro_46.
IPR023099. Glyco_hydro_46_N.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF01374. Glyco_hydro_46. 1 hit.
[Graphical view]
PIRSFiPIRSF036551. Chitosanase. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS60000. CHITOSANASE_46_80. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHMSNARPSK SRTKFLLAFL CFTLMASLFG ATALFGPSKA AAASPDDNFS
60 70 80 90 100
PETLQFLRNN TGLDGEQWNN IMKLINKPEQ DDLNWIKYYG YCEDIEDERG
110 120 130 140 150
YTIGLFGATT GGSRDTHPDG PDLFKAYDAA KGASNPSADG ALKRLGINGK
160 170 180 190 200
MKGSILEIKD SEKVFCGKIK KLQNDAAWRK AMWETFYNVY IRYSVEQARQ
210 220 230 240 250
RGFTSAVTIG SFVDTALNQG ATGGSDTLQG LLARSGSSSN EKTFMKNFHA
260 270 280 290 300
KRTLVVDTNK YNKPPNGKNR VKQWDTLVDM GKMNLKNVDS EIAQVTDWEM

K
Length:301
Mass (Da):33,426
Last modified:March 1, 2004 - v2
Checksum:i5B67FB8FF1D1F763
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10624 Genomic DNA. Translation: BAA01474.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10624 Genomic DNA. Translation: BAA01474.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QGIX-ray1.60A43-301[»]
2D05X-ray2.00A43-301[»]
ProteinModelPortaliP33673.
SMRiP33673.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB01296. Glucosamine.

Protein family/group databases

CAZyiGH46. Glycoside Hydrolase Family 46.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17652.
BRENDAi3.2.1.132. 649.

Miscellaneous databases

EvolutionaryTraceiP33673.

Family and domain databases

CDDicd00978. chitosanase_glyco_hydro_46. 1 hit.
Gene3Di3.30.386.10. 1 hit.
InterProiIPR000400. Glyco_hydro_46.
IPR023099. Glyco_hydro_46_N.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF01374. Glyco_hydro_46. 1 hit.
[Graphical view]
PIRSFiPIRSF036551. Chitosanase. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS60000. CHITOSANASE_46_80. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHIS_BACCI
AccessioniPrimary (citable) accession number: P33673
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: March 1, 2004
Last modified: November 2, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.