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Protein

Proteasome subunit beta type-3

Gene

PSMB3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-BTA-1169091. Activation of NF-kappaB in B cells.
R-BTA-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-BTA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-BTA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-BTA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-BTA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-BTA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-BTA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-BTA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-BTA-195253. Degradation of beta-catenin by the destruction complex.
R-BTA-202424. Downstream TCR signaling.
R-BTA-2467813. Separation of Sister Chromatids.
R-BTA-2871837. FCERI mediated NF-kB activation.
R-BTA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-BTA-350562. Regulation of ornithine decarboxylase (ODC).
R-BTA-382556. ABC-family proteins mediated transport.
R-BTA-446652. Interleukin-1 signaling.
R-BTA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-BTA-4608870. Asymmetric localization of PCP proteins.
R-BTA-4641257. Degradation of AXIN.
R-BTA-4641258. Degradation of DVL.
R-BTA-5358346. Hedgehog ligand biogenesis.
R-BTA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-BTA-5607764. CLEC7A (Dectin-1) signaling.
R-BTA-5610780. Degradation of GLI1 by the proteasome.
R-BTA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-BTA-5632684. Hedgehog 'on' state.
R-BTA-5658442. Regulation of RAS by GAPs.
R-BTA-5668541. TNFR2 non-canonical NF-kB pathway.
R-BTA-5676590. NIK-->noncanonical NF-kB signaling.
R-BTA-5687128. MAPK6/MAPK4 signaling.
R-BTA-5689603. UCH proteinases.
R-BTA-5689880. Ub-specific processing proteases.
R-BTA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-BTA-68949. Orc1 removal from chromatin.
R-BTA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-BTA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-BTA-69481. G2/M Checkpoints.
R-BTA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-BTA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-BTA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-BTA-8941858. Regulation of RUNX3 expression and activity.
R-BTA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiT01.983.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-3 (EC:3.4.25.1By similarity)
Alternative name(s):
Proteasome chain 13
Proteasome component C10-II
Proteasome theta chain
Gene namesi
Name:PSMB3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001480562 – 205Proteasome subunit beta type-3Add BLAST204

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei77N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP33672.
PeptideAtlasiP33672.
PRIDEiP33672.

Expressioni

Gene expression databases

BgeeiENSBTAG00000003830.
ExpressionAtlasiP33672. baseline.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7.1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004984.

Structurei

Secondary structure

1205
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 15Combined sources6
Beta strandi17 – 25Combined sources9
Beta strandi28 – 30Combined sources3
Beta strandi33 – 37Combined sources5
Beta strandi42 – 44Combined sources3
Beta strandi49 – 52Combined sources4
Helixi57 – 77Combined sources21
Beta strandi78 – 80Combined sources3
Helixi84 – 96Combined sources13
Turni97 – 100Combined sources4
Beta strandi108 – 112Combined sources5
Beta strandi119 – 124Combined sources6
Beta strandi130 – 132Combined sources3
Beta strandi134 – 140Combined sources7
Helixi143 – 153Combined sources11
Helixi160 – 174Combined sources15
Helixi175 – 177Combined sources3
Beta strandi185 – 200Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IRUX-ray2.75J/X1-205[»]
ProteinModelPortaliP33672.
SMRiP33672.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33672.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0180. Eukaryota.
ENOG410XSC8. LUCA.
GeneTreeiENSGT00550000074820.
HOGENOMiHOG000090523.
HOVERGENiHBG004446.
InParanoidiP33672.
KOiK02735.
OMAiEQLFGMC.
OrthoDBiEOG091G0JTX.
TreeFamiTF106216.

Family and domain databases

CDDicd03759. proteasome_beta_type_3. 1 hit.
Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR033811. Proteasome_beta_3.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
PANTHERiPTHR11599:SF70. PTHR11599:SF70. 1 hit.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33672-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIMSYNGGA VMAMKGKNCV AIAADRRFGI QAQMVTTDFQ KIFPMGDRLY
60 70 80 90 100
IGLAGLATDV QTVAQRLKFR LNLYELKEGR QIKPYTLMSM VANLLYEKRF
110 120 130 140 150
GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCTEQMYGMC
160 170 180 190 200
ESLWEPNMDP EHLFETISQA MLNAVDRDAV SGMGVIVHII EKDKITTRTL

KARMD
Length:205
Mass (Da):22,993
Last modified:June 13, 2006 - v3
Checksum:i62551E484D5042FD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89S → C AA sequence (PubMed:1510924).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102554 mRNA. Translation: AAI02555.1.
PIRiA42762.
B42762.
C42762.
D42762.
G42762.
RefSeqiNP_001029768.1. NM_001034596.2.
UniGeneiBt.44351.

Genome annotation databases

EnsembliENSBTAT00000004984; ENSBTAP00000004984; ENSBTAG00000003830.
GeneIDi533874.
KEGGibta:533874.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPSB3_BOVIN
AccessioniPrimary (citable) accession number: P33672
Secondary accession number(s): Q3T059
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 13, 2006
Last modified: June 7, 2017
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families