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Protein

tRNA 2-selenouridine synthase

Gene

selU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA.1 Publication

Catalytic activityi

5-methylaminomethyl-2-thiouridine + selenophosphate = 5-methylaminomethyl-2-selenouridine + phosphate.

Absorptioni

Abs(max)=258 nm

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei97 – 971S-selanylcysteine intermediateCurated

GO - Molecular functioni

  • tRNA 2-selenouridine synthase activity Source: EcoCyc

GO - Biological processi

  • tRNA seleno-modification Source: InterPro
  • tRNA wobble uridine modification Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Selenium

Enzyme and pathway databases

BioCyciEcoCyc:EG11768-MONOMER.
ECOL316407:JW0491-MONOMER.
MetaCyc:EG11768-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA 2-selenouridine synthase (EC:2.9.1.-)
Alternative name(s):
Selenophosphate-dependent tRNA 2-selenouridine synthase
Gene namesi
Name:selU
Synonyms:ybbB
Ordered Locus Names:b0503, JW0491
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11768. selU.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi96 – 961C → S: No change in activity. 1 Publication
Mutagenesisi97 – 971C → S in vivo, abolishes 2-selenouridine synthase activity. In vitro, reduces activity by only 50%. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364tRNA 2-selenouridine synthasePRO_0000210859Add
BLAST

Proteomic databases

PaxDbiP33667.
PRIDEiP33667.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4259870. 6 interactions.
IntActiP33667. 2 interactions.
STRINGi511145.b0503.

Structurei

3D structure databases

ProteinModelPortaliP33667.
SMRiP33667. Positions 11-134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 137124RhodaneseAdd
BLAST

Sequence similaritiesi

Belongs to the 2-selenouridine synthase family.Curated
Contains 1 rhodanese domain.Curated

Phylogenomic databases

eggNOGiENOG4105CR7. Bacteria.
COG2603. LUCA.
HOGENOMiHOG000260000.
InParanoidiP33667.
KOiK06917.
OMAiLHRGWIE.
OrthoDBiEOG6M3PB3.
PhylomeDBiP33667.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
HAMAPiMF_01622. tRNA_sel_U_synth.
InterProiIPR001763. Rhodanese-like_dom.
IPR017582. tRNA_2-selenouridine_synthase.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 2 hits.
TIGRFAMsiTIGR03167. tRNA_sel_U_synt. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33667-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQERHTEQDY RALLIADTPI IDVRAPIEFE HGAMPAAINL PLMNNDERAA
60 70 80 90 100
VGTCYKQQGS DAALALGHKL VAGEIRQQRM DAWRAACLQN PQGILCCARG
110 120 130 140 150
GQRSHIVQSW LHAAGIDYPL VEGGYKALRQ TAIQATIELA QKPIVLIGGC
160 170 180 190 200
TGSGKTLLVQ QQPNGVDLEG LARHRGSAFG RTLQPQLSQA SFENLLAAEM
210 220 230 240 250
LKTDARQNLR LWVLEDESRM IGSNHLPECL RERMTQAAIA VVEDPFEIRL
260 270 280 290 300
ERLNEEYFLR MHHDFTHAYG DEQGWQEYCE YLHHGLSAIK RRLGLQRYNE
310 320 330 340 350
LAARLDAALT TQLTTGSTDG HLAWLVPLLE EYYDPMYRYQ LEKKAEKVVF
360
RGEWAEVAEW VKAR
Length:364
Mass (Da):41,111
Last modified:November 1, 1997 - v2
Checksum:i8C0F244BA86B6C4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82664 Genomic DNA. Translation: AAB40256.1.
U00096 Genomic DNA. Translation: AAC73605.1.
AP009048 Genomic DNA. Translation: BAE76281.1.
X60999 Genomic DNA. Translation: CAA43313.1.
PIRiF64781.
RefSeqiNP_415036.1. NC_000913.3.
WP_001157938.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73605; AAC73605; b0503.
BAE76281; BAE76281; BAE76281.
GeneIDi947063.
KEGGiecj:JW0491.
eco:b0503.
PATRICi32116165. VBIEscCol129921_0524.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82664 Genomic DNA. Translation: AAB40256.1.
U00096 Genomic DNA. Translation: AAC73605.1.
AP009048 Genomic DNA. Translation: BAE76281.1.
X60999 Genomic DNA. Translation: CAA43313.1.
PIRiF64781.
RefSeqiNP_415036.1. NC_000913.3.
WP_001157938.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP33667.
SMRiP33667. Positions 11-134.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259870. 6 interactions.
IntActiP33667. 2 interactions.
STRINGi511145.b0503.

Proteomic databases

PaxDbiP33667.
PRIDEiP33667.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73605; AAC73605; b0503.
BAE76281; BAE76281; BAE76281.
GeneIDi947063.
KEGGiecj:JW0491.
eco:b0503.
PATRICi32116165. VBIEscCol129921_0524.

Organism-specific databases

EchoBASEiEB1718.
EcoGeneiEG11768. selU.

Phylogenomic databases

eggNOGiENOG4105CR7. Bacteria.
COG2603. LUCA.
HOGENOMiHOG000260000.
InParanoidiP33667.
KOiK06917.
OMAiLHRGWIE.
OrthoDBiEOG6M3PB3.
PhylomeDBiP33667.

Enzyme and pathway databases

BioCyciEcoCyc:EG11768-MONOMER.
ECOL316407:JW0491-MONOMER.
MetaCyc:EG11768-MONOMER.

Miscellaneous databases

PROiP33667.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
HAMAPiMF_01622. tRNA_sel_U_synth.
InterProiIPR001763. Rhodanese-like_dom.
IPR017582. tRNA_2-selenouridine_synthase.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 2 hits.
TIGRFAMsiTIGR03167. tRNA_sel_U_synt. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The RhsD-E subfamily of Escherichia coli K-12."
    Sadosky A.B., Gray J.A., Hill C.W.
    Nucleic Acids Res. 19:7177-7183(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 268-364.
    Strain: K12.
  5. "Functional diversity of the rhodanese homology domain: the Escherichia coli ybbB gene encodes a selenophosphate-dependent tRNA 2-selenouridine synthase."
    Wolfe M.D., Ahmed F., Lacourciere G.M., Lauhon C.T., Stadtman T.C., Larson T.J.
    J. Biol. Chem. 279:1801-1809(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF CYS-96 AND CYS-97.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiSELU_ECOLI
AccessioniPrimary (citable) accession number: P33667
Secondary accession number(s): P77709, Q2MBS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: January 20, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.