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Protein

Chitosanase

Gene

csn

Organism
Streptomyces sp. (strain N174)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Aids in the defense against invading fungal pathogens by degrading their cell wall chitosan.

Catalytic activityi

Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei62Proton donor1
Active sitei80Nucleophile1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.132. 1284.
SABIO-RKP33665.

Protein family/group databases

CAZyiGH46. Glycoside Hydrolase Family 46.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitosanase (EC:3.2.1.132)
Gene namesi
Name:csn
Synonyms:chs
OrganismiStreptomyces sp. (strain N174)
Taxonomic identifieri69019 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi62E → D or Q: Drastic reduction of activity. 1 Publication1
Mutagenesisi80D → E or N: Drastic reduction of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 401 PublicationAdd BLAST40
ChainiPRO_000001220941 – 278ChitosanaseAdd BLAST238

Structurei

Secondary structure

1278
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi44 – 46Combined sources3
Helixi48 – 62Combined sources15
Beta strandi63 – 66Combined sources4
Helixi68 – 71Combined sources4
Beta strandi75 – 77Combined sources3
Beta strandi79 – 82Combined sources4
Beta strandi84 – 86Combined sources3
Turni87 – 90Combined sources4
Turni93 – 95Combined sources3
Helixi97 – 108Combined sources12
Helixi115 – 117Combined sources3
Helixi118 – 124Combined sources7
Helixi134 – 144Combined sources11
Helixi148 – 161Combined sources14
Helixi163 – 172Combined sources10
Helixi177 – 190Combined sources14
Beta strandi192 – 195Combined sources4
Helixi199 – 209Combined sources11
Helixi213 – 215Combined sources3
Helixi219 – 236Combined sources18
Helixi244 – 247Combined sources4
Helixi250 – 255Combined sources6
Beta strandi265 – 269Combined sources5
Beta strandi272 – 276Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CHKX-ray2.40A/B41-278[»]
ProteinModelPortaliP33665.
SMRiP33665.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33665.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 46 family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd00978. chitosanase_glyco_hydro_46. 1 hit.
Gene3Di3.30.386.10. 1 hit.
InterProiIPR000400. Glyco_hydro_46.
IPR023099. Glyco_hydro_46_N.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF01374. Glyco_hydro_46. 1 hit.
[Graphical view]
PIRSFiPIRSF036551. Chitosanase. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS60000. CHITOSANASE_46_80. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33665-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSQHRTARI ALAVVLTAIP ASLATAGVGY ASTQASTAVK AGAGLDDPHK
60 70 80 90 100
KEIAMELVSS AENSSLDWKA QYKYIEDIGD GRGYTGGIIG FCSGTGDMLE
110 120 130 140 150
LVQHYTDLEP GNILAKYLPA LKKVNGSASH SGLGTPFTKD WATAAKDTVF
160 170 180 190 200
QQAQNDERDR VYFDPAVSQA KADGLRALGQ FAYYDAIVMH GPGNDPTSFG
210 220 230 240 250
GIRKTAMKKA RTPAQGGDET TYLNAFLDAR KAAMLTEAAH DDTSRVDTEQ
260 270
RVFLKAGNLD LNPPLKWKTY GDPYVINS
Length:278
Mass (Da):29,835
Last modified:February 1, 1994 - v1
Checksum:iAEB36286AA9AF95F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07779 Genomic DNA. Translation: AAA19865.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07779 Genomic DNA. Translation: AAA19865.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CHKX-ray2.40A/B41-278[»]
ProteinModelPortaliP33665.
SMRiP33665.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH46. Glycoside Hydrolase Family 46.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.2.1.132. 1284.
SABIO-RKP33665.

Miscellaneous databases

EvolutionaryTraceiP33665.

Family and domain databases

CDDicd00978. chitosanase_glyco_hydro_46. 1 hit.
Gene3Di3.30.386.10. 1 hit.
InterProiIPR000400. Glyco_hydro_46.
IPR023099. Glyco_hydro_46_N.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF01374. Glyco_hydro_46. 1 hit.
[Graphical view]
PIRSFiPIRSF036551. Chitosanase. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS60000. CHITOSANASE_46_80. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHIS_STRSN
AccessioniPrimary (citable) accession number: P33665
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.