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Protein

Ferrous iron transport protein B

Gene

feoB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-driven Fe2+ uptake system.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTPPROSITE-ProRule annotation
Nucleotide bindingi35 – 406GTPPROSITE-ProRule annotation
Nucleotide bindingi56 – 594GTPPROSITE-ProRule annotation
Nucleotide bindingi91 – 944GTPPROSITE-ProRule annotation
Nucleotide bindingi120 – 1234GTP

GO - Molecular functioni

  • ferrous iron transmembrane transporter activity Source: EcoCyc
  • GTP binding Source: EcoCyc
  • identical protein binding Source: IntAct

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • ferrous iron transmembrane transport Source: GOC
  • ferrous iron transport Source: EcoCyc
  • iron ion homeostasis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

GTP-binding, Iron, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:FEOB-MONOMER.
ECOL316407:JW3372-MONOMER.
MetaCyc:FEOB-MONOMER.

Protein family/group databases

TCDBi9.A.8.1.1. the ferrous iron uptake (feob) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferrous iron transport protein B
Gene namesi
Name:feoB
Ordered Locus Names:b3409, JW3372
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12102. feoB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 101101PeriplasmicSequence analysisAdd
BLAST
Transmembranei102 – 12221HelicalSequence analysisAdd
BLAST
Topological domaini123 – 281159CytoplasmicSequence analysisAdd
BLAST
Transmembranei282 – 30221HelicalSequence analysisAdd
BLAST
Topological domaini303 – 3086PeriplasmicSequence analysis
Transmembranei309 – 32921HelicalSequence analysisAdd
BLAST
Topological domaini330 – 3334CytoplasmicSequence analysis
Transmembranei334 – 35421HelicalSequence analysisAdd
BLAST
Topological domaini355 – 38228PeriplasmicSequence analysisAdd
BLAST
Transmembranei383 – 40321HelicalSequence analysisAdd
BLAST
Topological domaini404 – 42623CytoplasmicSequence analysisAdd
BLAST
Transmembranei427 – 44721HelicalSequence analysisAdd
BLAST
Topological domaini448 – 4525PeriplasmicSequence analysis
Transmembranei453 – 47321HelicalSequence analysisAdd
BLAST
Topological domaini474 – 4818CytoplasmicSequence analysis
Transmembranei482 – 50221HelicalSequence analysisAdd
BLAST
Topological domaini503 – 51715PeriplasmicSequence analysisAdd
BLAST
Transmembranei518 – 53821HelicalSequence analysisAdd
BLAST
Topological domaini539 – 56729CytoplasmicSequence analysisAdd
BLAST
Transmembranei568 – 58821HelicalSequence analysisAdd
BLAST
Topological domaini589 – 66375PeriplasmicSequence analysisAdd
BLAST
Transmembranei664 – 68421HelicalSequence analysisAdd
BLAST
Topological domaini685 – 6906CytoplasmicSequence analysis
Transmembranei691 – 71121HelicalSequence analysisAdd
BLAST
Topological domaini712 – 72110PeriplasmicSequence analysis
Transmembranei722 – 74221HelicalSequence analysisAdd
BLAST
Topological domaini743 – 77331CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi94 – 941D → N: No effect. 1 Publication
Mutagenesisi123 – 1231D → N: Loss of guanine nucleotide specificity and Fe(2+) uptake. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 773773Ferrous iron transport protein BPRO_0000210821Add
BLAST

Proteomic databases

PaxDbiP33650.

Expressioni

Inductioni

Iron uptake is repressed by the global regulator Fur. Induced by hydroxyurea (PubMed:20005847).1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-7131497,EBI-7131497

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4261727. 22 interactions.
DIPiDIP-9591N.
MINTiMINT-7914741.
STRINGi511145.b3409.

Structurei

Secondary structure

1
773
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 107Combined sources
Helixi16 – 249Combined sources
Beta strandi28 – 325Combined sources
Beta strandi36 – 4611Combined sources
Beta strandi51 – 566Combined sources
Beta strandi66 – 683Combined sources
Helixi72 – 8312Combined sources
Beta strandi87 – 948Combined sources
Helixi95 – 973Combined sources
Helixi98 – 11114Combined sources
Beta strandi115 – 1206Combined sources
Helixi122 – 1276Combined sources
Beta strandi130 – 1323Combined sources
Helixi134 – 1418Combined sources
Beta strandi145 – 1473Combined sources
Helixi151 – 1533Combined sources
Helixi154 – 16411Combined sources
Helixi179 – 19113Combined sources
Helixi198 – 21013Combined sources
Helixi213 – 2186Combined sources
Helixi220 – 2256Combined sources
Helixi226 – 23611Combined sources
Helixi240 – 25920Combined sources
Beta strandi260 – 2623Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HYRX-ray2.20A/B/C1-270[»]
3HYTX-ray2.74A/B/C1-270[»]
3I8SX-ray1.80A/B/C1-274[»]
3I8XX-ray2.25A/B/C1-274[»]
3I92X-ray3.00A/B/C1-274[»]
4Q00X-ray2.10A/B/C1-261[»]
4Q5IX-ray2.80A/B/C/D/E/F1-270[»]
4R98X-ray2.22A/B1-270[»]
ProteinModelPortaliP33650.
SMRiP33650. Positions 1-261.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33650.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 169167FeoB-type GPROSITE-ProRule annotationAdd
BLAST

Domaini

Contains a guanine-nucleotide-specific nucleotide-binding site that shows slow GTP hydrolysis.

Sequence similaritiesi

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. FeoB GTPase (TC 9.A.8) family. [View classification]PROSITE-ProRule annotation
Contains 1 FeoB-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CE1. Bacteria.
COG0370. LUCA.
HOGENOMiHOG000054023.
InParanoidiP33650.
KOiK04759.
OMAiFVLLYMP.
OrthoDBiEOG6JQH16.
PhylomeDBiP33650.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003373. Fe2_transport_prot-B.
IPR011640. Fe2_transport_prot_B_C.
IPR011619. Fe2_transport_prot_B_N.
IPR030389. G_FEOB_dom.
IPR011642. Gate_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11649:SF37. PTHR11649:SF37. 3 hits.
PfamiPF07664. FeoB_C. 1 hit.
PF02421. FeoB_N. 1 hit.
PF07670. Gate. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00437. feoB. 1 hit.
PROSITEiPS51711. G_FEOB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33650-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLTIGLIG NPNSGKTTLF NQLTGSRQRV GNWAGVTVER KEGQFSTTDH
60 70 80 90 100
QVTLVDLPGT YSLTTISSQT SLDEQIACHY ILSGDADLLI NVVDASNLER
110 120 130 140 150
NLYLTLQLLE LGIPCIVALN MLDIAEKQNI RIEIDALSAR LGCPVIPLVS
160 170 180 190 200
TRGRGIEALK LAIDRYKANE NVELVHYAQP LLNEADSLAK VMPSDIPLKQ
210 220 230 240 250
RRWLGLQMLE GDIYSRAYAG EASQHLDAAL ARLRNEMDDP ALHIADARYQ
260 270 280 290 300
CIAAICDVVS NTLTAEPSRF TTAVDKIVLN RFLGLPIFLF VMYLMFLLAI
310 320 330 340 350
NIGGALQPLF DVGSVALFVH GIQWIGYTLH FPDWLTIFLA QGLGGGINTV
360 370 380 390 400
LPLVPQIGMM YLFLSFLEDS GYMARAAFVM DRLMQALGLP GKSFVPLIVG
410 420 430 440 450
FGCNVPSVMG ARTLDAPRER LMTIMMAPFM SCGARLAIFA VFAAAFFGQN
460 470 480 490 500
GALAVFSLYM LGIVMAVLTG LMLKYTIMRG EATPFVMELP VYHVPHVKSL
510 520 530 540 550
IIQTWQRLKG FVLRAGKVII IVSIFLSAFN SFSLSGKIVD NINDSALASV
560 570 580 590 600
SRVITPVFKP IGVHEDNWQA TVGLFTGAMA KEVVVGTLNT LYTAENIQDE
610 620 630 640 650
EFNPAEFNLG EELFSAIDET WQSLKDTFSL SVLMNPIEAS KGDGEMGTGA
660 670 680 690 700
MGVMDQKFGS AAAAYSYLIF VLLYVPCISV MGAIARESSR GWMGFSILWG
710 720 730 740 750
LNIAYSLATL FYQVASYSQH PTYSLVCILA VILFNIVVIG LLRRARSRVD
760 770
IELLATRKSV SSCCAASTTG DCH
Length:773
Mass (Da):84,474
Last modified:February 1, 1994 - v1
Checksum:i1150A14B131AB9AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71063 Genomic DNA. Translation: CAA50387.1.
U18997 Genomic DNA. Translation: AAA58207.1.
U00096 Genomic DNA. Translation: AAC76434.1.
AP009048 Genomic DNA. Translation: BAE77882.1.
AY625107 Genomic DNA. Translation: AAT42461.1.
PIRiA36932.
RefSeqiNP_417868.1. NC_000913.3.
WP_000737039.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76434; AAC76434; b3409.
BAE77882; BAE77882; BAE77882.
GeneIDi947919.
KEGGiecj:JW3372.
eco:b3409.
PATRICi32122254. VBIEscCol129921_3504.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71063 Genomic DNA. Translation: CAA50387.1.
U18997 Genomic DNA. Translation: AAA58207.1.
U00096 Genomic DNA. Translation: AAC76434.1.
AP009048 Genomic DNA. Translation: BAE77882.1.
AY625107 Genomic DNA. Translation: AAT42461.1.
PIRiA36932.
RefSeqiNP_417868.1. NC_000913.3.
WP_000737039.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HYRX-ray2.20A/B/C1-270[»]
3HYTX-ray2.74A/B/C1-270[»]
3I8SX-ray1.80A/B/C1-274[»]
3I8XX-ray2.25A/B/C1-274[»]
3I92X-ray3.00A/B/C1-274[»]
4Q00X-ray2.10A/B/C1-261[»]
4Q5IX-ray2.80A/B/C/D/E/F1-270[»]
4R98X-ray2.22A/B1-270[»]
ProteinModelPortaliP33650.
SMRiP33650. Positions 1-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261727. 22 interactions.
DIPiDIP-9591N.
MINTiMINT-7914741.
STRINGi511145.b3409.

Protein family/group databases

TCDBi9.A.8.1.1. the ferrous iron uptake (feob) family.

Proteomic databases

PaxDbiP33650.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76434; AAC76434; b3409.
BAE77882; BAE77882; BAE77882.
GeneIDi947919.
KEGGiecj:JW3372.
eco:b3409.
PATRICi32122254. VBIEscCol129921_3504.

Organism-specific databases

EchoBASEiEB2026.
EcoGeneiEG12102. feoB.

Phylogenomic databases

eggNOGiENOG4105CE1. Bacteria.
COG0370. LUCA.
HOGENOMiHOG000054023.
InParanoidiP33650.
KOiK04759.
OMAiFVLLYMP.
OrthoDBiEOG6JQH16.
PhylomeDBiP33650.

Enzyme and pathway databases

BioCyciEcoCyc:FEOB-MONOMER.
ECOL316407:JW3372-MONOMER.
MetaCyc:FEOB-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP33650.
PROiP33650.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003373. Fe2_transport_prot-B.
IPR011640. Fe2_transport_prot_B_C.
IPR011619. Fe2_transport_prot_B_N.
IPR030389. G_FEOB_dom.
IPR011642. Gate_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11649:SF37. PTHR11649:SF37. 3 hits.
PfamiPF07664. FeoB_C. 1 hit.
PF02421. FeoB_N. 1 hit.
PF07670. Gate. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00437. feoB. 1 hit.
PROSITEiPS51711. G_FEOB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the ferrous iron uptake system of Escherichia coli."
    Kammler M., Schoen C., Hantke K.
    J. Bacteriol. 175:6212-6219(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations."
    Lenski R.E., Winkworth C.L., Riley M.A.
    J. Mol. Evol. 56:498-508(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 536-704.
    Strain: B / Bc251.
  5. "The membrane protein FeoB contains an intramolecular G protein essential for Fe(II) uptake in bacteria."
    Marlovits T.C., Haase W., Herrmann C., Aller S.G., Unger V.M.
    Proc. Natl. Acad. Sci. U.S.A. 99:16243-16248(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GTP-BINDING SITES, MUTAGENESIS OF ASP-94 AND ASP-123.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia coli."
    Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J., Walker G.C.
    Mol. Cell 36:845-860(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HYDROXYUREA.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiFEOB_ECOLI
AccessioniPrimary (citable) accession number: P33650
Secondary accession number(s): Q2M774, Q6IU42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 20, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Binding of GTP is required for an efficient Fe2+ uptake.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.