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Protein

Endoribonuclease ChpB

Gene

chpB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a type II toxin-antitoxin (TA) module. ChpB is a sequence-specific mRNA and (weak) tmRNA endoribonuclease that inhibits protein synthesis and induces bacterial stasis. Cleavage is independent of the ribosome. Cleavage occurs at ACY sequences where Y is not C. The endoribonuclease activity is not as strong as that of MazF. The endoribonuclease activity (a toxin) is inhibited by its labile cognate antitoxin ChpS. Toxicity results when the levels of ChpS decrease in the cell, leading to mRNA degradation. Both ChpS and ChpB probably bind to the promoter region of the chpS-chpB operon to autoregulate their synthesis.4 Publications

Enzyme regulationi

Stimulated in vitro in a concentration-dependent fashion by extracellular death factor (EDF, a quorum sensing pentapeptide sequence NNWNN, probably produced from the zwf gene product glucose-6-phosphate 1-dehydrogenase), which is able to overcome inhibition by cognate antitoxin ChpS.1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • endoribonuclease activity Source: UniProtKB
  • endoribonuclease activity, producing 5'-phosphomonoesters Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • negative regulation of cell growth Source: EcoCyc
  • regulation of mRNA stability Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Toxin

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12096-MONOMER.
ECOL316407:JW4184-MONOMER.
MetaCyc:EG12096-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease ChpB (EC:3.1.-.-)
Alternative name(s):
Toxin ChpB
mRNA interferase ChpB
Gene namesi
Name:chpB
Synonyms:chpBK, yjfE
Ordered Locus Names:b4225, JW4184
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12096. chpB.

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002018991 – 116Endoribonuclease ChpBAdd BLAST116

Proteomic databases

PaxDbiP33647.
PRIDEiP33647.

Expressioni

Inductioni

Part of the chpS-chpB operon.1 Publication

Interactioni

Subunit structurei

Homodimer, interacts with ChpS, which inhibits the endoribonuclease activity.1 Publication

Protein-protein interaction databases

BioGridi4259311. 4 interactors.
STRINGi511145.b4225.

Structurei

3D structure databases

ProteinModelPortaliP33647.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PemK/MazF family.Curated

Phylogenomic databases

eggNOGiENOG4105MZG. Bacteria.
COG2337. LUCA.
HOGENOMiHOG000290185.
InParanoidiP33647.
KOiK18841.
OMAiMIDYIER.
PhylomeDBiP33647.

Family and domain databases

Gene3Di2.30.30.110. 1 hit.
InterProiIPR003477. PemK-like.
IPR011067. Plasmid_toxin/cell-grow_inhib.
[Graphical view]
PfamiPF02452. PemK_toxin. 1 hit.
[Graphical view]
SUPFAMiSSF50118. SSF50118. 1 hit.

Sequencei

Sequence statusi: Complete.

P33647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKKSEFERG DIVLVGFDPA SGHEQQGAGR PALVLSVQAF NQLGMTLVAP
60 70 80 90 100
ITQGGNFARY AGFSVPLHCE EGDVHGVVLV NQVRMMDLHA RLAKRIGLAA
110
DEVVEEALLR LQAVVE
Length:116
Mass (Da):12,492
Last modified:February 1, 1994 - v1
Checksum:i4D641F3E302FCF58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16451 Genomic DNA. Translation: BAA03920.1.
M14018 Genomic DNA. No translation available.
U14003 Genomic DNA. Translation: AAA97122.1.
U00096 Genomic DNA. Translation: AAC77182.1.
M23550 Unassigned DNA. No translation available.
AP009048 Genomic DNA. Translation: BAE78226.1.
PIRiD49339.
RefSeqiNP_418646.1. NC_000913.3.
WP_000239577.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77182; AAC77182; b4225.
BAE78226; BAE78226; BAE78226.
GeneIDi948747.
KEGGiecj:JW4184.
eco:b4225.
PATRICi32124025. VBIEscCol129921_4357.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16451 Genomic DNA. Translation: BAA03920.1.
M14018 Genomic DNA. No translation available.
U14003 Genomic DNA. Translation: AAA97122.1.
U00096 Genomic DNA. Translation: AAC77182.1.
M23550 Unassigned DNA. No translation available.
AP009048 Genomic DNA. Translation: BAE78226.1.
PIRiD49339.
RefSeqiNP_418646.1. NC_000913.3.
WP_000239577.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP33647.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259311. 4 interactors.
STRINGi511145.b4225.

Proteomic databases

PaxDbiP33647.
PRIDEiP33647.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77182; AAC77182; b4225.
BAE78226; BAE78226; BAE78226.
GeneIDi948747.
KEGGiecj:JW4184.
eco:b4225.
PATRICi32124025. VBIEscCol129921_4357.

Organism-specific databases

EchoBASEiEB2020.
EcoGeneiEG12096. chpB.

Phylogenomic databases

eggNOGiENOG4105MZG. Bacteria.
COG2337. LUCA.
HOGENOMiHOG000290185.
InParanoidiP33647.
KOiK18841.
OMAiMIDYIER.
PhylomeDBiP33647.

Enzyme and pathway databases

BioCyciEcoCyc:EG12096-MONOMER.
ECOL316407:JW4184-MONOMER.
MetaCyc:EG12096-MONOMER.

Miscellaneous databases

PROiP33647.

Family and domain databases

Gene3Di2.30.30.110. 1 hit.
InterProiIPR003477. PemK-like.
IPR011067. Plasmid_toxin/cell-grow_inhib.
[Graphical view]
PfamiPF02452. PemK_toxin. 1 hit.
[Graphical view]
SUPFAMiSSF50118. SSF50118. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCHPB_ECOLI
AccessioniPrimary (citable) accession number: P33647
Secondary accession number(s): Q2M680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.