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Protein

Endoribonuclease toxin ChpB

Gene

chpB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Toxic component of a type II toxin-antitoxin (TA) system. ChpB is a sequence-specific mRNA and (weak) tmRNA endoribonuclease that inhibits protein synthesis and induces bacterial stasis. Cleavage is independent of the ribosome. Cleavage occurs at ACY sequences where Y is not C. The endoribonuclease activity is not as strong as that of MazF. The endoribonuclease activity (a toxin) is inhibited by its labile cognate antitoxin ChpS. Toxicity results when the levels of ChpS decrease in the cell, leading to mRNA degradation. Both ChpS and ChpB probably bind to the promoter region of the chpS-chpB operon to autoregulate their synthesis. mRNA interferases play a role in bacterial persistence to antibiotics (PubMed:21788497).5 Publications

Enzyme regulationi

Stimulated in vitro in a concentration-dependent fashion by extracellular death factor (EDF, a quorum sensing pentapeptide sequence NNWNN, probably produced from the zwf gene product glucose-6-phosphate 1-dehydrogenase), which is able to overcome inhibition by cognate antitoxin ChpS.1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • endoribonuclease activity Source: UniProtKB
  • endoribonuclease activity, producing 5'-phosphomonoesters Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • negative regulation of cell growth Source: EcoCyc
  • regulation of mRNA stability Source: UniProtKB

Keywordsi

Molecular functionDNA-binding, Endonuclease, Hydrolase, Nuclease, RNA-binding
Biological processToxin-antitoxin system

Enzyme and pathway databases

BioCyciEcoCyc:EG12096-MONOMER
MetaCyc:EG12096-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease toxin ChpB (EC:3.1.-.-)
Alternative name(s):
Toxin ChpB
mRNA interferase ChpB
Gene namesi
Name:chpB
Synonyms:chpBK, yjfE
Ordered Locus Names:b4225, JW4184
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG12096 chpB

Pathology & Biotechi

Disruption phenotypei

No visible phenotype (PubMed:8083180, PubMed:12972253). mRNA interferases play a role in bacterial persistence to antibiotics; as 10 mRNA interferases are successively deleted reduced levels of persisters are generated (PubMed:21788497).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002018991 – 116Endoribonuclease toxin ChpBAdd BLAST116

Proteomic databases

PaxDbiP33647
PRIDEiP33647

Expressioni

Inductioni

Part of the chpS-chpB operon.1 Publication

Interactioni

Subunit structurei

Homodimer, interacts with ChpS, which inhibits the endoribonuclease activity.1 Publication

Protein-protein interaction databases

BioGridi4259311, 4 interactors
STRINGi316385.ECDH10B_4420

Structurei

3D structure databases

ProteinModelPortaliP33647
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PemK/MazF family.Curated

Phylogenomic databases

eggNOGiENOG4105MZG Bacteria
COG2337 LUCA
HOGENOMiHOG000290185
InParanoidiP33647
KOiK18841
OMAiRVISMID
PhylomeDBiP33647

Family and domain databases

Gene3Di2.30.30.110, 1 hit
InterProiView protein in InterPro
IPR003477 PemK-like
IPR011067 Plasmid_toxin/cell-grow_inhib
PfamiView protein in Pfam
PF02452 PemK_toxin, 1 hit

Sequencei

Sequence statusi: Complete.

P33647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKKSEFERG DIVLVGFDPA SGHEQQGAGR PALVLSVQAF NQLGMTLVAP
60 70 80 90 100
ITQGGNFARY AGFSVPLHCE EGDVHGVVLV NQVRMMDLHA RLAKRIGLAA
110
DEVVEEALLR LQAVVE
Length:116
Mass (Da):12,492
Last modified:February 1, 1994 - v1
Checksum:i4D641F3E302FCF58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16451 Genomic DNA Translation: BAA03920.1
M14018 Genomic DNA No translation available.
U14003 Genomic DNA Translation: AAA97122.1
U00096 Genomic DNA Translation: AAC77182.1
M23550 Unassigned DNA No translation available.
AP009048 Genomic DNA Translation: BAE78226.1
PIRiD49339
RefSeqiNP_418646.1, NC_000913.3
WP_000239577.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77182; AAC77182; b4225
BAE78226; BAE78226; BAE78226
GeneIDi948747
KEGGiecj:JW4184
eco:b4225
PATRICifig|511145.12.peg.4357

Similar proteinsi

Entry informationi

Entry nameiCHPB_ECOLI
AccessioniPrimary (citable) accession number: P33647
Secondary accession number(s): Q2M680
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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