Reviewed, UniProtKB/Swiss-Prot P33647 (CHPB_ECOLI)

Names and origin

Protein names

Recommended name:
    PemK-like protein 2

Gene names

Name:	chpB
Synonyms:	chpBK, yjfE
Ordered Locus Names:	b4225, JW4184

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in the regulation of cell growth. It acts as a growth inhibitor. Both chpS and chpB bind to the promoter region of the chpSB operon to autoregulate their synthesis.
Sequence similarities	Belongs to the pemK family.

Ontologies

Keywords
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of cell growth Ref.1 Inferred from mutant phenotype. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW endoribonuclease activity, producing 5'-phosphomonoesters Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 116	116	PemK-like protein 2		PRO_0000201899

Sequences

	Sequence		Length	Mass (Da)
	P33647-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 4D641F3E302FCF58	FASTA	116	12,492
	10 20 30 40 50 60 MVKKSEFERG DIVLVGFDPA SGHEQQGAGR PALVLSVQAF NQLGMTLVAP ITQGGNFARY 70 80 90 100 110 AGFSVPLHCE EGDVHGVVLV NQVRMMDLHA RLAKRIGLAA DEVVEEALLR LQAVVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"chpA and chpB, Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100." Masuda Y., Miyakawa K., Nishimura Y., Ohtsubo E. J. Bacteriol. 175:6850-6856(1993) [PubMed: 8226627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Structural analysis of the ileR locus of Escherichia coli K12." Weiss D.L., Johnson D.I., Weith H.L., Somerville R.L. J. Biol. Chem. 261:9966-9971(1986) [PubMed: 3525538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30. Strain: K12.
[6]	"Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12." Lahti R., Pitkaeranta T., Valve E., Ilta I., Kukko-Kalske E., Heinonen J. J. Bacteriol. 170:5901-5907(1988) [PubMed: 2848015] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-116. Strain: K12.
[7]	"Mapping and disruption of the chpB locus in Escherichia coli." Masuda Y., Ohtsubo E. J. Bacteriol. 176:5861-5863(1994) [PubMed: 8083180] [Abstract] Cited for: GENE MAPPING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16451 Genomic DNA. Translation: BAA03920.1. M14018 Genomic DNA. No translation available. U14003 Genomic DNA. Translation: AAA97122.1. U00096 Genomic DNA. Translation: AAC77182.1. M23550 Unassigned DNA. No translation available. AP009048 Genomic DNA. Translation: BAE78226.1.
PIR	D49339.
RefSeq	AP_004725.1. NP_418646.1.
3D structure databases
SMR	P33647. Positions 7-114.
ModBase	Search...
Protein-protein interaction databases
STRING	P33647.
Genome annotation databases
GeneID	948747.
GenomeReviews	Gene locus JW4184 in contig AP009048_GR. Gene locus b4225 in contig U00096_GR.
KEGG	ecj:JW4184. eco:b4225.
Organism-specific databases
EchoBASE	EB2020.
EcoGene	EG12096. chpB.
CMR	Search...
Phylogenomic databases
eggNOG	COG2337.
HOGENOM	HBG571026.
OMA	GAKRVES.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12096-MONOMER. ECOL168927:B4225-MONOMER.
Gene expression databases
Genevestigator	P33647.
Family and domain databases
InterPro	IPR003477. PemK. IPR011067. Plasmid_toxin/cell-grow_inhib. [Graphical view]
Gene3D	G3DSA:2.30.30.110. CcdB. 1 hit.
Pfam	PF02452. PemK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHPB_ECOLI

Accession

Primary (citable) accession number: P33647
Secondary accession number(s): Q2M680

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)