P33647 (CHPB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
mRNA interferase ChpB
EC=3.1.-.-

Alternative name(s):
Endoribonuclease ChpB
Toxin ChpB

Gene names

Name:	chpB
Synonyms:	chpBK, yjfE
Ordered Locus Names:	b4225, JW4184

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Toxic component of a toxin-antitoxin (TA) module. ChpB is a sequence-specific mRNA and (weak) tmRNA endoribonuclease that inhibits protein synthesis and induces bacterial stasis. Cleavage is independent of the ribosome. Cleavage occurs at ACY sequences where Y is not C. The endoribonuclease activity is not as strong as that of MazF. The endoribonuclease activity (a toxin) is inhibited by the labile cognate antitoxin ChpS. Toxicity results when the levels of ChpS decrease in the cell, leading to mRNA degradation. Both ChpS and ChpB probably bind to the promoter region of the chpSB operon to autoregulate their synthesis. Ref.1 Ref.9
Subunit structure	Homodimer, interacts with ChpS, which inhibits the endoribonuclease activity. Ref.9
Induction	Part of the chpS-chpB operon.
Disruption phenotype	No visible phenotype. Ref.7 Ref.8
Sequence similarities	Belongs to the PemK/MazF family.

Ontologies

Keywords
Ligand	DNA-binding RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease Toxin
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of cell growth Inferred from mutant phenotype Ref.1. Source: EcoCyc regulation of mRNA stability Inferred from direct assay Ref.8. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW endoribonuclease activity, producing 5'-phosphomonoesters Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 116	116	mRNA interferase ChpB		PRO_0000201899

Sequences

	Sequence		Length	Mass (Da)
	P33647 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 4D641F3E302FCF58	FASTA	116	12,492
	10 20 30 40 50 60 MVKKSEFERG DIVLVGFDPA SGHEQQGAGR PALVLSVQAF NQLGMTLVAP ITQGGNFARY 70 80 90 100 110 AGFSVPLHCE EGDVHGVVLV NQVRMMDLHA RLAKRIGLAA DEVVEEALLR LQAVVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"chpA and chpB, Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100." Masuda Y., Miyakawa K., Nishimura Y., Ohtsubo E. J. Bacteriol. 175:6850-6856(1993) [PubMed: 8226627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PROBABLE OPERON STRUCTURE. Strain: K12 / MC1000 / ATCC 39531.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Structural analysis of the ileR locus of Escherichia coli K12." Weiss D.L., Johnson D.I., Weith H.L., Somerville R.L. J. Biol. Chem. 261:9966-9971(1986) [PubMed: 3525538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30. Strain: K12.
[6]	"Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12." Lahti R., Pitkaeranta T., Valve E., Ilta I., Kukko-Kalske E., Heinonen J. J. Bacteriol. 170:5901-5907(1988) [PubMed: 2848015] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-116. Strain: K12.
[7]	"Mapping and disruption of the chpB locus in Escherichia coli." Masuda Y., Ohtsubo E. J. Bacteriol. 176:5861-5863(1994) [PubMed: 8083180] [Abstract] Cited for: GENE MAPPING, DISRUPTION PHENOTYPE. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]	"Toxin-antitoxin loci as stress-response-elements: ChpAK/MazF and ChpBK cleave translated RNAs and are counteracted by tmRNA." Christensen S.K., Pedersen K., Hansen F.G., Gerdes K. J. Mol. Biol. 332:809-819(2003) [PubMed: 12972253] [Abstract] Cited for: RNA CLEAVAGE, DISRUPTION PHENOTYPE. Strain: K12 / MG1655 / ATCC 47076.
[9]	"Model for RNA binding and the catalytic site of the RNase Kid of the bacterial parD toxin-antitoxin system." Kamphuis M.B., Bonvin A.M., Monti M.C., Lemonnier M., Munoz-Gomez A., van den Heuvel R.H., Diaz-Orejas R., Boelens R. J. Mol. Biol. 357:115-126(2006) [PubMed: 16413033] [Abstract] Cited for: FUNCTION AS AN RNA ENDORIBONUCLEASE, SUBUNIT, INTERACTION WITH CHPS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16451 Genomic DNA. Translation: BAA03920.1. M14018 Genomic DNA. No translation available. U14003 Genomic DNA. Translation: AAA97122.1. U00096 Genomic DNA. Translation: AAC77182.1. M23550 Unassigned DNA. No translation available. AP009048 Genomic DNA. Translation: BAE78226.1.
PIR	D49339.
RefSeq	NP_418646.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P33647.
SMR	P33647. Positions 7-115.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001618; EBESCP00000001618; EBESCG00000001334. EBESCT00000001619; EBESCP00000001619; EBESCG00000001334. EBESCT00000016193; EBESCP00000015484; EBESCG00000015253.
GeneID	948747.
GenomeReviews	Gene locus JW4184 in contig AP009048_GR. Gene locus b4225 in contig U00096_GR.
KEGG	ecj:JW4184. eco:b4225.
PATRIC	32124025. VBIEscCol129921_4357.
Organism-specific databases
EchoBASE	EB2020.
EcoGene	EG12096. chpB.
Phylogenomic databases
eggNOG	COG2337.
GeneTree	EBGT00050000009044.
HOGENOM	HBG571026.
OMA	VNEILAM.
PhylomeDB	P33647.
ProtClustDB	PRK09812.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12096-MONOMER. MetaCyc:EG12096-MONOMER.
Gene expression databases
Genevestigator	P33647.
Family and domain databases
InterPro	IPR003477. PemK. IPR011067. Plasmid_toxin/cell-grow_inhib. [Graphical view]
Gene3D	G3DSA:2.30.30.110. CcdB. 1 hit.
KO	K07171.
Pfam	PF02452. PemK. 1 hit. [Graphical view]
SUPFAM	SSF50118. Plasmid_toxin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CHPB_ECOLI

Accession

Primary (citable) accession number: P33647
Secondary accession number(s): Q2M680

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program