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P33647 (CHPB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
mRNA interferase ChpB

EC=3.1.-.-
Alternative name(s):
Endoribonuclease ChpB
Toxin ChpB
Gene names
Name:chpB
Synonyms:chpBK, yjfE
Ordered Locus Names:b4225, JW4184
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length116 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Toxic component of a toxin-antitoxin (TA) module. ChpB is a sequence-specific mRNA and (weak) tmRNA endoribonuclease that inhibits protein synthesis and induces bacterial stasis. Cleavage is independent of the ribosome. Cleavage occurs at ACY sequences where Y is not C. The endoribonuclease activity is not as strong as that of MazF. The endoribonuclease activity (a toxin) is inhibited by the labile cognate antitoxin ChpS. Toxicity results when the levels of ChpS decrease in the cell, leading to mRNA degradation. Both ChpS and ChpB probably bind to the promoter region of the chpSB operon to autoregulate their synthesis. Ref.1 Ref.9

Subunit structure

Homodimer, interacts with ChpS, which inhibits the endoribonuclease activity. Ref.9

Induction

Part of the chpS-chpB operon.

Disruption phenotype

No visible phenotype. Ref.7 Ref.8

Sequence similarities

Belongs to the PemK/MazF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 116116mRNA interferase ChpB
PRO_0000201899

Sequences

Sequence LengthMass (Da)Tools
P33647 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 4D641F3E302FCF58

FASTA11612,492
        10         20         30         40         50         60 
MVKKSEFERG DIVLVGFDPA SGHEQQGAGR PALVLSVQAF NQLGMTLVAP ITQGGNFARY 

        70         80         90        100        110 
AGFSVPLHCE EGDVHGVVLV NQVRMMDLHA RLAKRIGLAA DEVVEEALLR LQAVVE 

« Hide

References

« Hide 'large scale' references
[1]"chpA and chpB, Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100."
Masuda Y., Miyakawa K., Nishimura Y., Ohtsubo E.
J. Bacteriol. 175:6850-6856(1993) [PubMed: 8226627] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PROBABLE OPERON STRUCTURE.
Strain: K12 / MC1000 / ATCC 39531.
[2]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Structural analysis of the ileR locus of Escherichia coli K12."
Weiss D.L., Johnson D.I., Weith H.L., Somerville R.L.
J. Biol. Chem. 261:9966-9971(1986) [PubMed: 3525538] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
Strain: K12.
[6]"Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12."
Lahti R., Pitkaeranta T., Valve E., Ilta I., Kukko-Kalske E., Heinonen J.
J. Bacteriol. 170:5901-5907(1988) [PubMed: 2848015] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-116.
Strain: K12.
[7]"Mapping and disruption of the chpB locus in Escherichia coli."
Masuda Y., Ohtsubo E.
J. Bacteriol. 176:5861-5863(1994) [PubMed: 8083180] [Abstract]
Cited for: GENE MAPPING, DISRUPTION PHENOTYPE.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]"Toxin-antitoxin loci as stress-response-elements: ChpAK/MazF and ChpBK cleave translated RNAs and are counteracted by tmRNA."
Christensen S.K., Pedersen K., Hansen F.G., Gerdes K.
J. Mol. Biol. 332:809-819(2003) [PubMed: 12972253] [Abstract]
Cited for: RNA CLEAVAGE, DISRUPTION PHENOTYPE.
Strain: K12 / MG1655 / ATCC 47076.
[9]"Model for RNA binding and the catalytic site of the RNase Kid of the bacterial parD toxin-antitoxin system."
Kamphuis M.B., Bonvin A.M., Monti M.C., Lemonnier M., Munoz-Gomez A., van den Heuvel R.H., Diaz-Orejas R., Boelens R.
J. Mol. Biol. 357:115-126(2006) [PubMed: 16413033] [Abstract]
Cited for: FUNCTION AS AN RNA ENDORIBONUCLEASE, SUBUNIT, INTERACTION WITH CHPS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16451 Genomic DNA. Translation: BAA03920.1.
M14018 Genomic DNA. No translation available.
U14003 Genomic DNA. Translation: AAA97122.1.
U00096 Genomic DNA. Translation: AAC77182.1.
M23550 Unassigned DNA. No translation available.
AP009048 Genomic DNA. Translation: BAE78226.1.
PIRD49339.
RefSeqNP_418646.1. NC_000913.2.

3D structure databases

ProteinModelPortalP33647.
SMRP33647. Positions 7-115.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000001618; EBESCP00000001618; EBESCG00000001334.
EBESCT00000001619; EBESCP00000001619; EBESCG00000001334.
EBESCT00000016193; EBESCP00000015484; EBESCG00000015253.
GeneID948747.
GenomeReviewsGene locus JW4184 in contig AP009048_GR.
Gene locus b4225 in contig U00096_GR.
KEGGecj:JW4184.
eco:b4225.
PATRIC32124025. VBIEscCol129921_4357.

Organism-specific databases

EchoBASEEB2020.
EcoGeneEG12096. chpB.

Phylogenomic databases

eggNOGCOG2337.
GeneTreeEBGT00050000009044.
HOGENOMHBG571026.
OMAVNEILAM.
PhylomeDBP33647.
ProtClustDBPRK09812.

Enzyme and pathway databases

BioCycEcoCyc:EG12096-MONOMER.
MetaCyc:EG12096-MONOMER.

Gene expression databases

GenevestigatorP33647.

Family and domain databases

InterProIPR003477. PemK.
IPR011067. Plasmid_toxin/cell-grow_inhib.
[Graphical view]
Gene3DG3DSA:2.30.30.110. CcdB. 1 hit.
KOK07171.
PfamPF02452. PemK. 1 hit.
[Graphical view]
SUPFAMSSF50118. Plasmid_toxin. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCHPB_ECOLI
AccessionPrimary (citable) accession number: P33647
Secondary accession number(s): Q2M680
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families