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Protein

Polyphenol oxidase

Gene

yfiH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multicopper oxidase with polyphenol oxidase activity. Is able to oxidize syringaldazine and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) in vitro. The physiological substrate and role of YfiH is not known.1 Publication

Cofactori

Cu cation1 PublicationNote: Binds 4 Cu cations per subunit.1 Publication

Kineticsi

kcat is 1450 min(-1) with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid as substrate. kcat is 21720 min(-1) with syringaldazine as substrate (at pH 4.5 and 40 degrees Celsius).1 Publication
  1. KM=23 µM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (at pH 4.5 and 40 degrees Celsius)1 Publication
  2. KM=1.10 µM for syringaldazine (at pH 4.5 and 40 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 5.5-8.4. Maintains 80% activity at pH 5.0-9.0.1 Publication

    Temperature dependencei

    Optimum temperature is 44 degrees Celsius. Maintains more than 80% activity at 50 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi35Copper 3By similarity1
    Metal bindingi71Copper 1By similarity1
    Metal bindingi107Copper 1By similarity1
    Metal bindingi124Copper 1By similarity1
    Metal bindingi216Copper 2By similarity1

    GO - Molecular functioni

    • copper ion binding Source: EcoCyc
    • oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor Source: UniProtKB
    • protein homodimerization activity Source: UniProtKB

    Keywordsi

    Molecular functionOxidoreductase
    LigandCopper, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG12097-MONOMER
    MetaCyc:EG12097-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyphenol oxidase1 Publication (EC:1.10.3.-1 Publication)
    Alternative name(s):
    Multicopper oxidase YfiH1 Publication
    Gene namesi
    Name:yfiH1 PublicationImported
    Ordered Locus Names:b2593, JW2575
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12097 yfiH

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene are more sensitive to ampicillin, cephradine and cefoxitin than wild-type.1 Publication

    Chemistry databases

    DrugBankiDB04272 Citric Acid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001631631 – 243Polyphenol oxidaseAdd BLAST243

    Proteomic databases

    EPDiP33644
    PaxDbiP33644
    PRIDEiP33644

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    GO - Molecular functioni

    • protein homodimerization activity Source: UniProtKB

    Protein-protein interaction databases

    BioGridi4260619, 654 interactors
    IntActiP33644, 8 interactors
    STRINGi316385.ECDH10B_2761

    Structurei

    Secondary structure

    1243
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi15 – 20Combined sources6
    Beta strandi38 – 41Combined sources4
    Helixi44 – 57Combined sources14
    Beta strandi75 – 77Combined sources3
    Beta strandi89 – 93Combined sources5
    Beta strandi99 – 114Combined sources16
    Beta strandi120 – 125Combined sources6
    Helixi127 – 132Combined sources6
    Helixi134 – 140Combined sources7
    Helixi146 – 148Combined sources3
    Beta strandi149 – 153Combined sources5
    Turni159 – 161Combined sources3
    Helixi166 – 175Combined sources10
    Helixi177 – 182Combined sources6
    Beta strandi183 – 186Combined sources4
    Beta strandi189 – 192Combined sources4
    Helixi194 – 204Combined sources11
    Beta strandi209 – 212Combined sources4
    Turni217 – 219Combined sources3
    Turni221 – 223Combined sources3
    Helixi227 – 230Combined sources4
    Beta strandi236 – 242Combined sources7

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1Z9TX-ray1.54A1-243[»]
    ProteinModelPortaliP33644
    SMRiP33644
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP33644

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105DBW Bacteria
    COG1496 LUCA
    HOGENOMiHOG000242994
    InParanoidiP33644
    KOiK05810
    OMAiVVMTADC
    PhylomeDBiP33644

    Family and domain databases

    CDDicd16833 YfiH, 1 hit
    Gene3Di3.60.140.10, 1 hit
    InterProiView protein in InterPro
    IPR003730 Cu_polyphenol_OxRdtase
    IPR038371 Cu_polyphenol_OxRdtase_sf
    IPR011324 Cytotoxic_necrot_fac-like_cat
    PANTHERiPTHR30616 PTHR30616, 1 hit
    PfamiView protein in Pfam
    PF02578 Cu-oxidase_4, 1 hit
    SUPFAMiSSF64438 SSF64438, 1 hit
    TIGRFAMsiTIGR00726 TIGR00726, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P33644-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSKLIVPQWP QPKGVAACSS TRIGGVSLPP YDSLNLGAHC GDNPDHVEEN
    60 70 80 90 100
    RKRLFAAGNL PSKPVWLEQV HGKDVLKLTG EPYASKRADA SYSNTPGTVC
    110 120 130 140 150
    AVMTADCLPV LFCNRAGTEV AAAHAGWRGL CAGVLEETVS CFADNPENIL
    160 170 180 190 200
    AWLGPAIGPR AFEVGGEVRE AFMAVDAKAS AAFIQHGDKY LADIYQLARQ
    210 220 230 240
    RLANVGVEQI FGGDRCTYTE NETFFSYRRD KTTGRMASFI WLI
    Length:243
    Mass (Da):26,339
    Last modified:February 1, 1994 - v1
    Checksum:iD21267A5374EAC43
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti27S → T in AAA92958 (Ref. 1) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U50134 Genomic DNA Translation: AAA92958.1
    X57620 Genomic DNA No translation available.
    U00096 Genomic DNA Translation: AAC75642.1
    AP009048 Genomic DNA Translation: BAA16477.1
    M29364 Genomic DNA Translation: AAA24421.1 Sequence problems.
    PIRiD65037
    RefSeqiNP_417084.1, NC_000913.3
    WP_000040169.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC75642; AAC75642; b2593
    BAA16477; BAA16477; BAA16477
    GeneIDi947089
    KEGGiecj:JW2575
    eco:b2593
    PATRICifig|511145.12.peg.2693

    Similar proteinsi

    Entry informationi

    Entry nameiPOLOX_ECOLI
    AccessioniPrimary (citable) accession number: P33644
    Secondary accession number(s): Q46989
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: March 28, 2018
    This is version 129 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health