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P33622 (APOC3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Apolipoprotein C-III

Short name=Apo-CIII
Short name=ApoC-III
Alternative name(s):
Apolipoprotein C3
Gene names
Name:Apoc3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length99 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.

Subcellular location

Secreted.

Tissue specificity

Mainly VLDL and also in HDL. Synthesized predominantly in liver and to a lesser degree in intestine.

Sequence similarities

Belongs to the apolipoprotein C3 family.

Mass spectrometry

Molecular mass is 8830.5±0.354 Da from positions 21 - 99. Determined by ESI. Strain C57BL/6. Without methionine sulfoxide. Ref.4

Molecular mass is 8891.4 Da from positions 21 - 99. Determined by ESI. Strain BALB/c. Without methionine sulfoxide. Ref.4

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
Lipid transport
Transport
   Cellular componentChylomicron
Secreted
VLDL
   DomainSignal
   PTMGlycoprotein
Oxidation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

cellular response to glucose stimulus

Inferred from electronic annotation. Source: Ensembl

cholesterol efflux

Inferred from electronic annotation. Source: Ensembl

cholesterol homeostasis

Inferred from electronic annotation. Source: Ensembl

cholesterol metabolic process

Inferred from mutant phenotype PubMed 11590213PubMed 8089130PubMed 8864964. Source: MGI

chylomicron remnant clearance

Inferred from electronic annotation. Source: Ensembl

high-density lipoprotein particle remodeling

Inferred from electronic annotation. Source: Ensembl

inflammatory response

Inferred from electronic annotation. Source: Ensembl

lipoprotein metabolic process

Inferred from electronic annotation. Source: InterPro

lipoprotein transport

Inferred from electronic annotation. Source: Ensembl

negative regulation of cholesterol import

Inferred from electronic annotation. Source: Ensembl

negative regulation of fatty acid biosynthetic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of high-density lipoprotein particle clearance

Inferred from electronic annotation. Source: Ensembl

negative regulation of lipoprotein lipase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of low-density lipoprotein particle clearance

Inferred from electronic annotation. Source: Ensembl

negative regulation of receptor-mediated endocytosis

Inferred from electronic annotation. Source: Ensembl

negative regulation of triglyceride catabolic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of very-low-density lipoprotein particle clearance

Inferred from electronic annotation. Source: Ensembl

phospholipid efflux

Inferred from electronic annotation. Source: Ensembl

regulation of Cdc42 protein signal transduction

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to nutrient

Inferred from electronic annotation. Source: Ensembl

response to peptide hormone

Inferred from electronic annotation. Source: Ensembl

triglyceride catabolic process

Inferred from mutant phenotype PubMed 11590213. Source: MGI

triglyceride homeostasis

Inferred from electronic annotation. Source: Ensembl

triglyceride metabolic process

Inferred from mutant phenotype PubMed 11590213PubMed 8089130. Source: MGI

triglyceride mobilization

Inferred from mutant phenotype PubMed 11551841PubMed 8864964. Source: MGI

   Cellular_componentchylomicron

Inferred from electronic annotation. Source: UniProtKB-KW

cytosol

Traceable author statement. Source: Reactome

extracellular region

Traceable author statement. Source: Reactome

intermediate-density lipoprotein particle

Inferred from electronic annotation. Source: Ensembl

spherical high-density lipoprotein particle

Inferred from electronic annotation. Source: Ensembl

very-low-density lipoprotein particle

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionlipase inhibitor activity

Inferred from electronic annotation. Source: Ensembl

phospholipid binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 9979Apolipoprotein C-III
PRO_0000002033

Regions

Region68 – 9932Lipid-binding By similarity

Amino acid modifications

Modified residue631Methionine sulfoxide
Glycosylation941O-linked (GalNAc...) By similarity

Experimental info

Sequence conflict571V → A in L04150. Ref.1
Sequence conflict691F → S in BAB22448. Ref.2
Sequence conflict691F → S in BAB25563. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P33622 [UniParc].

Last modified November 7, 2003. Version 2.
Checksum: BBC1690C055ADEC7

FASTA9910,982
        10         20         30         40         50         60 
MQPRTLLTVA LLALLASARA EEVEGSLLLG SVQGYMEQAS KTVQDALSSV QESDIAVVAR 

        70         80         90 
GWMDNHFRFL KGYWSKFTDK FTGFWDSNPE DQPTPAIES 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the mouse apolipoprotein Apoa-1/Apoc-3 gene locus: genomic, mRNA, and protein sequences with comparisons to other species."
Januzzi J.L., Azrolan N., O'Connell A., Aalto-Setala K., Breslow J.L.
Genomics 14:1081-1088(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney and Small intestine.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[4]"Mass spectral analysis of the apolipoproteins on mouse high density lipoproteins. Detection of post-translational modifications."
Puppione D.L., Yam L.M., Bassilian S., Souda P., Castellani L.W., Schumaker V.N., Whitelegge J.P.
Biochim. Biophys. Acta 1764:1363-1371(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 42-68, MASS SPECTROMETRY, OXIDATION AT MET-63 TO METHIONINE SULFOXIDE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L04149 Genomic DNA. No translation available.
L04150 mRNA. No translation available.
AK002908 mRNA. Translation: BAB22448.1.
AK008260 mRNA. Translation: BAB25563.1.
BC021776 mRNA. Translation: AAH21776.1.
PIRB44364.
RefSeqNP_075603.1. NM_023114.4.
UniGeneMm.390161.

3D structure databases

ProteinModelPortalP33622.
SMRP33622. Positions 22-99.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP33622. 1 interaction.
MINTMINT-1858244.

PTM databases

PhosphoSiteP33622.

Proteomic databases

PaxDbP33622.
PRIDEP33622.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034586; ENSMUSP00000034586; ENSMUSG00000032081.
ENSMUST00000118649; ENSMUSP00000113058; ENSMUSG00000032081.
GeneID11814.
KEGGmmu:11814.

Organism-specific databases

CTD345.
MGIMGI:88055. Apoc3.

Phylogenomic databases

eggNOGNOG39866.
GeneTreeENSGT00390000015395.
HOGENOMHOG000247042.
HOVERGENHBG050549.
InParanoidP33622.
KOK08759.
PhylomeDBP33622.
TreeFamTF338209.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.
REACT_189085. Disease.

Gene expression databases

BgeeP33622.
CleanExMM_APOC3.
GenevestigatorP33622.

Family and domain databases

InterProIPR008403. Apo-CIII.
[Graphical view]
PANTHERPTHR14225. PTHR14225. 1 hit.
PfamPF05778. Apo-CIII. 1 hit.
[Graphical view]
ProDomPD010414. Apo-CIII. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other

ChiTaRSAPOC3. mouse.
NextBio279695.
PROP33622.
SOURCESearch...

Entry information

Entry nameAPOC3_MOUSE
AccessionPrimary (citable) accession number: P33622
Secondary accession number(s): Q8VC58, Q9CPP9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 7, 2003
Last modified: April 16, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot