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Protein

Apolipoprotein C-III

Gene

Apoc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Lipid transport, Transport

Keywords - Ligandi

Sialic acid

Enzyme and pathway databases

ReactomeiREACT_295031. Retinoid metabolism and transport.
REACT_314625. Chylomicron-mediated lipid transport.
REACT_326379. HDL-mediated lipid transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein C-III
Short name:
Apo-CIII
Short name:
ApoC-III
Alternative name(s):
Apolipoprotein C3
Gene namesi
Name:Apoc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88055. Apoc3.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chylomicron, Secreted, VLDL

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 9979Apolipoprotein C-IIIPRO_0000002033Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei63 – 631Methionine sulfoxide1 Publication
Glycosylationi94 – 941O-linked (GalNAc...)By similarity

Post-translational modificationi

The most abundant glycoforms are characterized by an O-linked disaccharide galactose linked to N-acetylgalactosamine (Gal-GalNAc), further modified with up to 3 sialic acid residues. Less abundant glycoforms are characterized by more complex and fucosylated glycan moieties. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.By similarity

Keywords - PTMi

Glycoprotein, Oxidation

Proteomic databases

MaxQBiP33622.
PaxDbiP33622.
PRIDEiP33622.

PTM databases

PhosphoSiteiP33622.

Expressioni

Gene expression databases

BgeeiP33622.
CleanExiMM_APOC3.
ExpressionAtlasiP33622. baseline and differential.
GenevisibleiP33622. MM.

Interactioni

Protein-protein interaction databases

IntActiP33622. 1 interaction.
MINTiMINT-1858244.
STRINGi10090.ENSMUSP00000034586.

Structurei

3D structure databases

ProteinModelPortaliP33622.
SMRiP33622. Positions 22-99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 9932Lipid-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the apolipoprotein C3 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG39866.
HOGENOMiHOG000247042.
HOVERGENiHBG050549.
InParanoidiP33622.
KOiK08759.
PhylomeDBiP33622.
TreeFamiTF338209.

Family and domain databases

InterProiIPR008403. Apo-CIII.
[Graphical view]
PANTHERiPTHR14225. PTHR14225. 1 hit.
PfamiPF05778. Apo-CIII. 1 hit.
[Graphical view]
ProDomiPD010414. Apo-CIII. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPRTLLTVA LLALLASARA EEVEGSLLLG SVQGYMEQAS KTVQDALSSV
60 70 80 90
QESDIAVVAR GWMDNHFRFL KGYWSKFTDK FTGFWDSNPE DQPTPAIES
Length:99
Mass (Da):10,982
Last modified:November 7, 2003 - v2
Checksum:iBBC1690C055ADEC7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571V → A in L04150 (PubMed:1478650).Curated
Sequence conflicti69 – 691F → S in BAB22448 (PubMed:16141072).Curated
Sequence conflicti69 – 691F → S in BAB25563 (PubMed:16141072).Curated

Mass spectrometryi

Molecular mass is 8830.5±0.354 Da from positions 21 - 99. Determined by ESI. Strain C57BL/6. Without methionine sulfoxide.1 Publication
Molecular mass is 8891.4 Da from positions 21 - 99. Determined by ESI. Strain BALB/c. Without methionine sulfoxide.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04149 Genomic DNA. No translation available.
L04150 mRNA. No translation available.
AK002908 mRNA. Translation: BAB22448.1.
AK008260 mRNA. Translation: BAB25563.1.
BC021776 mRNA. Translation: AAH21776.1.
CCDSiCCDS23141.1.
PIRiB44364.
RefSeqiNP_001276685.1. NM_001289756.1.
NP_075603.1. NM_023114.4.
UniGeneiMm.390161.

Genome annotation databases

GeneIDi11814.
KEGGimmu:11814.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04149 Genomic DNA. No translation available.
L04150 mRNA. No translation available.
AK002908 mRNA. Translation: BAB22448.1.
AK008260 mRNA. Translation: BAB25563.1.
BC021776 mRNA. Translation: AAH21776.1.
CCDSiCCDS23141.1.
PIRiB44364.
RefSeqiNP_001276685.1. NM_001289756.1.
NP_075603.1. NM_023114.4.
UniGeneiMm.390161.

3D structure databases

ProteinModelPortaliP33622.
SMRiP33622. Positions 22-99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP33622. 1 interaction.
MINTiMINT-1858244.
STRINGi10090.ENSMUSP00000034586.

PTM databases

PhosphoSiteiP33622.

Proteomic databases

MaxQBiP33622.
PaxDbiP33622.
PRIDEiP33622.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi11814.
KEGGimmu:11814.

Organism-specific databases

CTDi345.
MGIiMGI:88055. Apoc3.

Phylogenomic databases

eggNOGiNOG39866.
HOGENOMiHOG000247042.
HOVERGENiHBG050549.
InParanoidiP33622.
KOiK08759.
PhylomeDBiP33622.
TreeFamiTF338209.

Enzyme and pathway databases

ReactomeiREACT_295031. Retinoid metabolism and transport.
REACT_314625. Chylomicron-mediated lipid transport.
REACT_326379. HDL-mediated lipid transport.

Miscellaneous databases

ChiTaRSiApoc3. mouse.
NextBioi279695.
PROiP33622.
SOURCEiSearch...

Gene expression databases

BgeeiP33622.
CleanExiMM_APOC3.
ExpressionAtlasiP33622. baseline and differential.
GenevisibleiP33622. MM.

Family and domain databases

InterProiIPR008403. Apo-CIII.
[Graphical view]
PANTHERiPTHR14225. PTHR14225. 1 hit.
PfamiPF05778. Apo-CIII. 1 hit.
[Graphical view]
ProDomiPD010414. Apo-CIII. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the mouse apolipoprotein Apoa-1/Apoc-3 gene locus: genomic, mRNA, and protein sequences with comparisons to other species."
    Januzzi J.L., Azrolan N., O'Connell A., Aalto-Setala K., Breslow J.L.
    Genomics 14:1081-1088(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney and Small intestine.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  4. "Mass spectral analysis of the apolipoproteins on mouse high density lipoproteins. Detection of post-translational modifications."
    Puppione D.L., Yam L.M., Bassilian S., Souda P., Castellani L.W., Schumaker V.N., Whitelegge J.P.
    Biochim. Biophys. Acta 1764:1363-1371(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 42-68, MASS SPECTROMETRY, OXIDATION AT MET-63.

Entry informationi

Entry nameiAPOC3_MOUSE
AccessioniPrimary (citable) accession number: P33622
Secondary accession number(s): Q8VC58, Q9CPP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 7, 2003
Last modified: June 24, 2015
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.