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Protein

Apolipoprotein C-III

Gene

Apoc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei41May interact with the LDL receptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processLipid degradation, Lipid metabolism, Lipid transport, Transport
LigandSialic acid

Enzyme and pathway databases

ReactomeiR-MMU-975634. Retinoid metabolism and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein C-III
Short name:
Apo-CIII
Short name:
ApoC-III
Alternative name(s):
Apolipoprotein C3
Gene namesi
Name:Apoc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88055. Apoc3.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Chylomicron, Secreted, VLDL

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000203321 – 99Apolipoprotein C-IIIAdd BLAST79

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei63Methionine sulfoxide1 Publication1
Glycosylationi94O-linked (GalNAc...) threonineBy similarity1

Post-translational modificationi

The most abundant glycoforms are characterized by an O-linked disaccharide galactose linked to N-acetylgalactosamine (Gal-GalNAc), further modified with up to 3 sialic acid residues. Less abundant glycoforms are characterized by more complex and fucosylated glycan moieties. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.By similarity

Keywords - PTMi

Glycoprotein, Oxidation

Proteomic databases

MaxQBiP33622.
PaxDbiP33622.
PeptideAtlasiP33622.
PRIDEiP33622.

PTM databases

iPTMnetiP33622.
PhosphoSitePlusiP33622.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032081.
CleanExiMM_APOC3.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiP33622. 1 interactor.
MINTiMINT-1858244.
STRINGi10090.ENSMUSP00000034586.

Structurei

3D structure databases

ProteinModelPortaliP33622.
SMRiP33622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 99Lipid-bindingBy similarityAdd BLAST32

Sequence similaritiesi

Belongs to the apolipoprotein C3 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JE3N. Eukaryota.
ENOG4111APE. LUCA.
HOGENOMiHOG000247042.
HOVERGENiHBG050549.
InParanoidiP33622.
KOiK08759.
PhylomeDBiP33622.
TreeFamiTF338209.

Family and domain databases

InterProiView protein in InterPro
IPR008403. Apo-CIII.
PANTHERiPTHR14225. PTHR14225. 1 hit.
PfamiView protein in Pfam
PF05778. Apo-CIII. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD010414. Apo-CIII. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPRTLLTVA LLALLASARA EEVEGSLLLG SVQGYMEQAS KTVQDALSSV
60 70 80 90
QESDIAVVAR GWMDNHFRFL KGYWSKFTDK FTGFWDSNPE DQPTPAIES
Length:99
Mass (Da):10,982
Last modified:November 7, 2003 - v2
Checksum:iBBC1690C055ADEC7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57V → A in L04150 (PubMed:1478650).Curated1
Sequence conflicti69F → S in BAB22448 (PubMed:16141072).Curated1
Sequence conflicti69F → S in BAB25563 (PubMed:16141072).Curated1

Mass spectrometryi

Molecular mass is 8830.5±0.354 Da from positions 21 - 99. Determined by ESI. Strain C57BL/6. Without methionine sulfoxide.1 Publication
Molecular mass is 8891.4 Da from positions 21 - 99. Determined by ESI. Strain BALB/c. Without methionine sulfoxide.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04149 Genomic DNA. No translation available.
L04150 mRNA. No translation available.
AK002908 mRNA. Translation: BAB22448.1.
AK008260 mRNA. Translation: BAB25563.1.
BC021776 mRNA. Translation: AAH21776.1.
CCDSiCCDS23141.1.
PIRiB44364.
RefSeqiNP_001276685.1. NM_001289756.1.
NP_075603.1. NM_023114.4.
UniGeneiMm.390161.

Genome annotation databases

GeneIDi11814.
KEGGimmu:11814.
UCSCiuc033jkf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04149 Genomic DNA. No translation available.
L04150 mRNA. No translation available.
AK002908 mRNA. Translation: BAB22448.1.
AK008260 mRNA. Translation: BAB25563.1.
BC021776 mRNA. Translation: AAH21776.1.
CCDSiCCDS23141.1.
PIRiB44364.
RefSeqiNP_001276685.1. NM_001289756.1.
NP_075603.1. NM_023114.4.
UniGeneiMm.390161.

3D structure databases

ProteinModelPortaliP33622.
SMRiP33622.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP33622. 1 interactor.
MINTiMINT-1858244.
STRINGi10090.ENSMUSP00000034586.

PTM databases

iPTMnetiP33622.
PhosphoSitePlusiP33622.

Proteomic databases

MaxQBiP33622.
PaxDbiP33622.
PeptideAtlasiP33622.
PRIDEiP33622.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi11814.
KEGGimmu:11814.
UCSCiuc033jkf.1. mouse.

Organism-specific databases

CTDi345.
MGIiMGI:88055. Apoc3.

Phylogenomic databases

eggNOGiENOG410JE3N. Eukaryota.
ENOG4111APE. LUCA.
HOGENOMiHOG000247042.
HOVERGENiHBG050549.
InParanoidiP33622.
KOiK08759.
PhylomeDBiP33622.
TreeFamiTF338209.

Enzyme and pathway databases

ReactomeiR-MMU-975634. Retinoid metabolism and transport.

Miscellaneous databases

ChiTaRSiApoc3. mouse.
PROiPR:P33622.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032081.
CleanExiMM_APOC3.

Family and domain databases

InterProiView protein in InterPro
IPR008403. Apo-CIII.
PANTHERiPTHR14225. PTHR14225. 1 hit.
PfamiView protein in Pfam
PF05778. Apo-CIII. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD010414. Apo-CIII. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPOC3_MOUSE
AccessioniPrimary (citable) accession number: P33622
Secondary accession number(s): Q8VC58, Q9CPP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 7, 2003
Last modified: May 10, 2017
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.