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P33619 (KLK3_MACMU) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Prostate-specific antigen

Short name=PSA
EC=3.4.21.77
Alternative name(s):
Kallikrein-3
Semenogelase
Gene names
Name:KLK3
Synonyms:APS
OrganismMacaca mulatta (Rhesus macaque)
Taxonomic identifier9544 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum By similarity.

Catalytic activity

Preferential cleavage: -Tyr-|-Xaa-.

Enzyme regulation

Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders By similarity.

Subunit structure

Forms heterodimer with SERPINA5 By similarity.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Propeptide19 – 246Activation peptide
PRO_0000027935
Chain25 – 261237Prostate-specific antigen
PRO_0000027936

Regions

Domain25 – 258234Peptidase S1

Sites

Active site651Charge relay system By similarity
Active site1201Charge relay system By similarity
Active site2131Charge relay system By similarity

Amino acid modifications

Glycosylation1021N-linked (GlcNAc...) Potential
Disulfide bond31 ↔ 173 By similarity
Disulfide bond50 ↔ 66 By similarity
Disulfide bond152 ↔ 219 By similarity
Disulfide bond184 ↔ 198 By similarity
Disulfide bond209 ↔ 234 By similarity

Sequences

Sequence LengthMass (Da)Tools
P33619 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 8525B14B15967E5C

FASTA26128,816
        10         20         30         40         50         60 
MWVLVVFLTL SVTWIGAAPL ILSRIVGGWE CEKHSQPWQV LVASRGRAVC GGVLVHPQWV 

        70         80         90        100        110        120 
LTAAHCIRSN SVILLGRHNP YYPEDTGQVF QVSHSFPHPL YNMSLLKNRY LGPGDDSSHD 

       130        140        150        160        170        180 
LMLLRLSEPA EITDAVQVLD LPTWEPELGT TCYASGWGSI EPEEHLTPKK LQCVDLHIIS 

       190        200        210        220        230        240 
NDVCAQVHSQ KVTKFMLCAG SWMGGKSTCS GDSGGPLVCD GVLQGITSWG SQPCALPRRP 

       250        260 
SLYTKVVRYR KWIQDTIMAN P 

« Hide

References

[1]"Characterization of rhesus monkey prostate specific antigen cDNA."
Gauthier E.R., Chapdelaine P., Tremblay R.R., Dube J.Y.
Biochim. Biophys. Acta 1174:207-210(1993) [PubMed: 7689340] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X73560 mRNA. Translation: CAA51957.1.
PIRS35711.
RefSeqNP_001036241.1. NM_001042776.1.
UniGeneMmu.3785.

3D structure databases

ProteinModelPortalP33619.
SMRP33619. Positions 25-261.
ModBaseSearch...

Protein-protein interaction databases

STRINGP33619.

Protein family/group databases

MEROPSS01.162.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID719444.
KEGGmcc:719444.

Organism-specific databases

CTD354.

Phylogenomic databases

eggNOGprNOG04669.
HOVERGENHBG013304.
InParanoidP33619.
OrthoDBEOG415GF4.

Enzyme and pathway databases

BRENDA3.4.21.77. 3126.

Family and domain databases

InterProIPR009003. Pept_cys/ser_Trypsin-like.
IPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]
KOK01351.
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. Pept_Ser_Cys. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKLK3_MACMU
AccessionPrimary (citable) accession number: P33619
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families