P33619 (KLK3_MACMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prostate-specific antigen
Short name=PSA
EC=3.4.21.77

Alternative name(s):
Kallikrein-3
Semenogelase

Gene names

Name:	KLK3
Synonyms:	APS

Organism

Macaca mulatta (Rhesus macaque)

Taxonomic identifier

9544 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum By similarity.
Catalytic activity	Preferential cleavage: -Tyr-|-Xaa-.
Enzyme regulation	Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders By similarity.
Subunit structure	Forms heterodimer with SERPINA5 By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 24	6	Activation peptide		PRO_0000027935
	Chain	25 – 261	237	Prostate-specific antigen		PRO_0000027936
Regions
	Domain	25 – 258	234	Peptidase S1
Sites
	Active site	65	1	Charge relay system By similarity
	Active site	120	1	Charge relay system By similarity
	Active site	213	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 173		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	152 ↔ 219		By similarity
	Disulfide bond	184 ↔ 198		By similarity
	Disulfide bond	209 ↔ 234		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33619 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 8525B14B15967E5C	FASTA	261	28,816
	10 20 30 40 50 60 MWVLVVFLTL SVTWIGAAPL ILSRIVGGWE CEKHSQPWQV LVASRGRAVC GGVLVHPQWV 70 80 90 100 110 120 LTAAHCIRSN SVILLGRHNP YYPEDTGQVF QVSHSFPHPL YNMSLLKNRY LGPGDDSSHD 130 140 150 160 170 180 LMLLRLSEPA EITDAVQVLD LPTWEPELGT TCYASGWGSI EPEEHLTPKK LQCVDLHIIS 190 200 210 220 230 240 NDVCAQVHSQ KVTKFMLCAG SWMGGKSTCS GDSGGPLVCD GVLQGITSWG SQPCALPRRP 250 260 SLYTKVVRYR KWIQDTIMAN P

References

[1]	"Characterization of rhesus monkey prostate specific antigen cDNA." Gauthier E.R., Chapdelaine P., Tremblay R.R., Dube J.Y. Biochim. Biophys. Acta 1174:207-210(1993) [PubMed: 7689340] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X73560 mRNA. Translation: CAA51957.1.
PIR	S35711.
RefSeq	NP_001036241.1. NM_001042776.1.
UniGene	Mmu.3785.
3D structure databases
ProteinModelPortal	P33619.
SMR	P33619. Positions 25-261.
ModBase	Search...
Protein-protein interaction databases
STRING	P33619.
Protein family/group databases
MEROPS	S01.162.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	719444.
KEGG	mcc:719444.
Organism-specific databases
CTD	354.
Phylogenomic databases
eggNOG	prNOG04669.
HOVERGEN	HBG013304.
InParanoid	P33619.
OrthoDB	EOG415GF4.
Enzyme and pathway databases
BRENDA	3.4.21.77. 3126.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
KO	K01351.
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KLK3_MACMU

Accession

Primary (citable) accession number: P33619

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program