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P33610

- PRI2_MOUSE

UniProt

P33610 - PRI2_MOUSE

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Protein

DNA primase large subunit

Gene
Prim2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.

Cofactori

Binds 1 4Fe-4S cluster By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi287 – 2871Iron-sulfur (4Fe-4S) By similarity
Metal bindingi367 – 3671Iron-sulfur (4Fe-4S) By similarity
Metal bindingi384 – 3841Iron-sulfur (4Fe-4S) By similarity
Metal bindingi424 – 4241Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA primase activity Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication, Transcription

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primase large subunit (EC:2.7.7.-)
Alternative name(s):
DNA primase 58 kDa subunit
Short name:
p58
Gene namesi
Name:Prim2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:97758. Prim2.

Subcellular locationi

GO - Cellular componenti

  1. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505DNA primase large subunitPRO_0000046769Add
BLAST

Proteomic databases

MaxQBiP33610.
PaxDbiP33610.
PRIDEiP33610.

PTM databases

PhosphoSiteiP33610.

Expressioni

Gene expression databases

BgeeiP33610.
CleanExiMM_PRIM2.
GenevestigatoriP33610.

Interactioni

Subunit structurei

Heterodimer of a small subunit and a large subunit.

Protein-protein interaction databases

BioGridi202362. 1 interaction.
IntActiP33610. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliP33610.
SMRiP33610. Positions 29-253, 271-455.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2219.
GeneTreeiENSGT00390000009790.
HOGENOMiHOG000212154.
HOVERGENiHBG008257.
InParanoidiP33610.
KOiK02685.
OMAiFVRAETM.
OrthoDBiEOG7WMCJ4.
PhylomeDBiP33610.
TreeFamiTF105893.

Family and domain databases

InterProiIPR016558. DNA_primase_lsu_euk.
IPR007238. DNA_primase_lsu_euk/arc.
[Graphical view]
PfamiPF04104. DNA_primase_lrg. 1 hit.
[Graphical view]
PIRSFiPIRSF009449. DNA_primase_large_subunit. 1 hit.

Sequencei

Sequence statusi: Complete.

P33610-1 [UniParc]FASTAAdd to Basket

« Hide

MQFSGRIRKK LRLAGDQRNA SYPHSLQFYL QPPTENISLT EFENLAFDRV    50
KLLKAIENLG VSYVKGTEQY QSKLEAEIRK LKFSYRENLE DEYEPRRRDH 100
ISHFILRLAY CQSEDLRRWF IQQEMDLLRF RFSILPKDKV QSFLKDSHLH 150
FEAISDEEKT LREQDIMASS PSLSGIKLES ESVYKVPFAD ALDLFRGRKV 200
YLEDGFAYVP LKDIVAIILN EFRATLSKAL ALTARSLPAV QSDERLQPLL 250
NHLSHSYTGQ DYSTQKNTGK ISLDQIDSLS TKSFPPCMRQ LHKALRENHH 300
LRHGGRMQYG LFLKGIGLTL EQALQFWKQE FIRGKMDPDK FDKGYSYNIR 350
HSFGKEGKRT DYTPFSCMKI ILTNPPGQGD YHGCPFRHSD AELLKQKMQS 400
YKIPASGISQ ILDLVKGNHY QVACQKYFEM THNVDDCGFS LNHPNQFFFE 450
SQRILTGGKD IKKEISQPET PQHKPSTQKT RDAASALASL DSSLEMDLEG 500
LEEYF 505
Length:505
Mass (Da):58,408
Last modified:February 1, 1994 - v1
Checksum:i36A64C77669D8C24
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091A → G in BAA04203. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13545 mRNA. Translation: BAA02745.1.
D17385 mRNA. Translation: BAA04203.1.
AK076095 mRNA. Translation: BAC36179.1.
AK077760 mRNA. Translation: BAC36996.1.
AK153970 mRNA. Translation: BAE32287.1.
BC019500 mRNA. Translation: AAH19500.1.
CCDSiCCDS14862.1.
PIRiC46642.
RefSeqiNP_032948.1. NM_008922.2.
UniGeneiMm.27705.

Genome annotation databases

EnsembliENSMUST00000027312; ENSMUSP00000027312; ENSMUSG00000026134.
GeneIDi19076.
KEGGimmu:19076.
UCSCiuc007anq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13545 mRNA. Translation: BAA02745.1 .
D17385 mRNA. Translation: BAA04203.1 .
AK076095 mRNA. Translation: BAC36179.1 .
AK077760 mRNA. Translation: BAC36996.1 .
AK153970 mRNA. Translation: BAE32287.1 .
BC019500 mRNA. Translation: AAH19500.1 .
CCDSi CCDS14862.1.
PIRi C46642.
RefSeqi NP_032948.1. NM_008922.2.
UniGenei Mm.27705.

3D structure databases

ProteinModelPortali P33610.
SMRi P33610. Positions 29-253, 271-455.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202362. 1 interaction.
IntActi P33610. 4 interactions.

PTM databases

PhosphoSitei P33610.

Proteomic databases

MaxQBi P33610.
PaxDbi P33610.
PRIDEi P33610.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027312 ; ENSMUSP00000027312 ; ENSMUSG00000026134 .
GeneIDi 19076.
KEGGi mmu:19076.
UCSCi uc007anq.1. mouse.

Organism-specific databases

CTDi 5558.
MGIi MGI:97758. Prim2.

Phylogenomic databases

eggNOGi COG2219.
GeneTreei ENSGT00390000009790.
HOGENOMi HOG000212154.
HOVERGENi HBG008257.
InParanoidi P33610.
KOi K02685.
OMAi FVRAETM.
OrthoDBi EOG7WMCJ4.
PhylomeDBi P33610.
TreeFami TF105893.

Miscellaneous databases

NextBioi 295610.
PROi P33610.
SOURCEi Search...

Gene expression databases

Bgeei P33610.
CleanExi MM_PRIM2.
Genevestigatori P33610.

Family and domain databases

InterProi IPR016558. DNA_primase_lsu_euk.
IPR007238. DNA_primase_lsu_euk/arc.
[Graphical view ]
Pfami PF04104. DNA_primase_lrg. 1 hit.
[Graphical view ]
PIRSFi PIRSF009449. DNA_primase_large_subunit. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the cDNAs for the four subunits of mouse DNA polymerase alpha-primase complex and their gene expression during cell proliferation and the cell cycle."
    Miyazawa H., Izumi M., Tada S., Takada R., Masutani M., Ui M., Hanaoka F.
    J. Biol. Chem. 268:8111-8122(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 30-48; 186-212 AND 403-416.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiPRI2_MOUSE
AccessioniPrimary (citable) accession number: P33610
Secondary accession number(s): Q3U4Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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