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Protein

DNA primase large subunit

Gene

Prim2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi287Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi367Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi384Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi424Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Transferase
Biological processDNA replication, Transcription
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-MMU-174411 Polymerase switching on the C-strand of the telomere
R-MMU-174430 Telomere C-strand synthesis initiation
R-MMU-68952 DNA replication initiation
R-MMU-68962 Activation of the pre-replicative complex
R-MMU-69091 Polymerase switching
R-MMU-69166 Removal of the Flap Intermediate
R-MMU-69183 Processive synthesis on the lagging strand

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primase large subunit (EC:2.7.7.-)
Alternative name(s):
DNA primase 58 kDa subunit
Short name:
p58
Gene namesi
Name:Prim2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:97758 Prim2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000467691 – 505DNA primase large subunitAdd BLAST505

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei470PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP33610
MaxQBiP33610
PaxDbiP33610
PeptideAtlasiP33610
PRIDEiP33610

PTM databases

iPTMnetiP33610
PhosphoSitePlusiP33610

Expressioni

Gene expression databases

BgeeiENSMUSG00000026134
CleanExiMM_PRIM2
GenevisibleiP33610 MM

Interactioni

Subunit structurei

Heterodimer of a small subunit and a large subunit.

Protein-protein interaction databases

BioGridi202362, 1 interactor
CORUMiP33610
IntActiP33610, 6 interactors
STRINGi10090.ENSMUSP00000027312

Structurei

3D structure databases

ProteinModelPortaliP33610
SMRiP33610
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2267 Eukaryota
COG2219 LUCA
GeneTreeiENSGT00390000009790
HOGENOMiHOG000212154
HOVERGENiHBG008257
InParanoidiP33610
KOiK02685
OMAiDCHGCPY
OrthoDBiEOG091G08G7
PhylomeDBiP33610
TreeFamiTF105893

Family and domain databases

CDDicd07322 PriL_PriS_Eukaryotic, 1 hit
InterProiView protein in InterPro
IPR016558 DNA_primase_lsu_euk
IPR007238 DNA_primase_lsu_euk/arc
PANTHERiPTHR10537 PTHR10537, 1 hit
PfamiView protein in Pfam
PF04104 DNA_primase_lrg, 1 hit
PIRSFiPIRSF009449 DNA_primase_large_subunit, 1 hit

Sequencei

Sequence statusi: Complete.

P33610-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFSGRIRKK LRLAGDQRNA SYPHSLQFYL QPPTENISLT EFENLAFDRV
60 70 80 90 100
KLLKAIENLG VSYVKGTEQY QSKLEAEIRK LKFSYRENLE DEYEPRRRDH
110 120 130 140 150
ISHFILRLAY CQSEDLRRWF IQQEMDLLRF RFSILPKDKV QSFLKDSHLH
160 170 180 190 200
FEAISDEEKT LREQDIMASS PSLSGIKLES ESVYKVPFAD ALDLFRGRKV
210 220 230 240 250
YLEDGFAYVP LKDIVAIILN EFRATLSKAL ALTARSLPAV QSDERLQPLL
260 270 280 290 300
NHLSHSYTGQ DYSTQKNTGK ISLDQIDSLS TKSFPPCMRQ LHKALRENHH
310 320 330 340 350
LRHGGRMQYG LFLKGIGLTL EQALQFWKQE FIRGKMDPDK FDKGYSYNIR
360 370 380 390 400
HSFGKEGKRT DYTPFSCMKI ILTNPPGQGD YHGCPFRHSD AELLKQKMQS
410 420 430 440 450
YKIPASGISQ ILDLVKGNHY QVACQKYFEM THNVDDCGFS LNHPNQFFFE
460 470 480 490 500
SQRILTGGKD IKKEISQPET PQHKPSTQKT RDAASALASL DSSLEMDLEG

LEEYF
Length:505
Mass (Da):58,408
Last modified:February 1, 1994 - v1
Checksum:i36A64C77669D8C24
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109A → G in BAA04203 (PubMed:8026492).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13545 mRNA Translation: BAA02745.1
D17385 mRNA Translation: BAA04203.1
AK076095 mRNA Translation: BAC36179.1
AK077760 mRNA Translation: BAC36996.1
AK153970 mRNA Translation: BAE32287.1
BC019500 mRNA Translation: AAH19500.1
CCDSiCCDS14862.1
PIRiC46642
RefSeqiNP_032948.1, NM_008922.2
UniGeneiMm.27705

Genome annotation databases

EnsembliENSMUST00000027312; ENSMUSP00000027312; ENSMUSG00000026134
GeneIDi19076
KEGGimmu:19076
UCSCiuc007anq.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPRI2_MOUSE
AccessioniPrimary (citable) accession number: P33610
Secondary accession number(s): Q3U4Z3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 31, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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