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P33610 (PRI2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA primase large subunit

EC=2.7.7.-
Alternative name(s):
DNA primase 58 kDa subunit
Short name=p58
Gene names
Name:Prim2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.

Cofactor

Binds 1 4Fe-4S cluster By similarity.

Subunit structure

Heterodimer of a small subunit and a large subunit.

Sequence similarities

Belongs to the eukaryotic-type primase large subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 505505DNA primase large subunit
PRO_0000046769

Sites

Metal binding2871Iron-sulfur (4Fe-4S) By similarity
Metal binding3671Iron-sulfur (4Fe-4S) By similarity
Metal binding3841Iron-sulfur (4Fe-4S) By similarity
Metal binding4241Iron-sulfur (4Fe-4S) By similarity

Experimental info

Sequence conflict1091A → G in BAA04203. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P33610 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 36A64C77669D8C24

FASTA50558,408
        10         20         30         40         50         60 
MQFSGRIRKK LRLAGDQRNA SYPHSLQFYL QPPTENISLT EFENLAFDRV KLLKAIENLG 

        70         80         90        100        110        120 
VSYVKGTEQY QSKLEAEIRK LKFSYRENLE DEYEPRRRDH ISHFILRLAY CQSEDLRRWF 

       130        140        150        160        170        180 
IQQEMDLLRF RFSILPKDKV QSFLKDSHLH FEAISDEEKT LREQDIMASS PSLSGIKLES 

       190        200        210        220        230        240 
ESVYKVPFAD ALDLFRGRKV YLEDGFAYVP LKDIVAIILN EFRATLSKAL ALTARSLPAV 

       250        260        270        280        290        300 
QSDERLQPLL NHLSHSYTGQ DYSTQKNTGK ISLDQIDSLS TKSFPPCMRQ LHKALRENHH 

       310        320        330        340        350        360 
LRHGGRMQYG LFLKGIGLTL EQALQFWKQE FIRGKMDPDK FDKGYSYNIR HSFGKEGKRT 

       370        380        390        400        410        420 
DYTPFSCMKI ILTNPPGQGD YHGCPFRHSD AELLKQKMQS YKIPASGISQ ILDLVKGNHY 

       430        440        450        460        470        480 
QVACQKYFEM THNVDDCGFS LNHPNQFFFE SQRILTGGKD IKKEISQPET PQHKPSTQKT 

       490        500 
RDAASALASL DSSLEMDLEG LEEYF 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of the cDNAs for the four subunits of mouse DNA polymerase alpha-primase complex and their gene expression during cell proliferation and the cell cycle."
Miyazawa H., Izumi M., Tada S., Takada R., Masutani M., Ui M., Hanaoka F.
J. Biol. Chem. 268:8111-8122(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 30-48; 186-212 AND 403-416.
[2]"DNA replication in vitro by recombinant DNA-polymerase-alpha-primase."
Stadlbauer F., Brueckner A., Rehfuess C., Eckerskorn C., Lottspeich F., Foerster V., Tseng B.Y., Nasheuer H.-P.
Eur. J. Biochem. 222:781-793(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Thymus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13545 mRNA. Translation: BAA02745.1.
D17385 mRNA. Translation: BAA04203.1.
AK076095 mRNA. Translation: BAC36179.1.
AK077760 mRNA. Translation: BAC36996.1.
AK153970 mRNA. Translation: BAE32287.1.
BC019500 mRNA. Translation: AAH19500.1.
CCDSCCDS14862.1.
PIRC46642.
RefSeqNP_032948.1. NM_008922.2.
UniGeneMm.27705.

3D structure databases

ProteinModelPortalP33610.
SMRP33610. Positions 29-253, 271-455.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202362. 1 interaction.
IntActP33610. 4 interactions.

PTM databases

PhosphoSiteP33610.

Proteomic databases

MaxQBP33610.
PaxDbP33610.
PRIDEP33610.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027312; ENSMUSP00000027312; ENSMUSG00000026134.
GeneID19076.
KEGGmmu:19076.
UCSCuc007anq.1. mouse.

Organism-specific databases

CTD5558.
MGIMGI:97758. Prim2.

Phylogenomic databases

eggNOGCOG2219.
GeneTreeENSGT00390000009790.
HOGENOMHOG000212154.
HOVERGENHBG008257.
InParanoidP33610.
KOK02685.
OMAFVRAETM.
OrthoDBEOG7WMCJ4.
PhylomeDBP33610.
TreeFamTF105893.

Gene expression databases

BgeeP33610.
CleanExMM_PRIM2.
GenevestigatorP33610.

Family and domain databases

InterProIPR016558. DNA_primase_lsu_euk.
IPR007238. DNA_primase_lsu_euk/arc.
[Graphical view]
PfamPF04104. DNA_primase_lrg. 1 hit.
[Graphical view]
PIRSFPIRSF009449. DNA_primase_large_subunit. 1 hit.
ProtoNetSearch...

Other

NextBio295610.
PROP33610.
SOURCESearch...

Entry information

Entry namePRI2_MOUSE
AccessionPrimary (citable) accession number: P33610
Secondary accession number(s): Q3U4Z3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot