Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P33602

- NUOG_ECOLI

UniProt

P33602 - NUOG_ECOLI

Protein

NADH-quinone oxidoreductase subunit G

Gene

nuoG

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.By similarity
    Binds 3 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi34 – 341Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi45 – 451Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi48 – 481Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi67 – 671Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi99 – 991Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
    Metal bindingi103 – 1031Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi106 – 1061Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi112 – 1121Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi151 – 1511Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi154 – 1541Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi157 – 1571Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi201 – 2011Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi228 – 2281Iron-sulfur 4 (4Fe-4S)By similarity
    Metal bindingi231 – 2311Iron-sulfur 4 (4Fe-4S)By similarity
    Metal bindingi235 – 2351Iron-sulfur 4 (4Fe-4S)By similarity
    Metal bindingi263 – 2631Iron-sulfur 4 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: EcoCyc
    2. 4 iron, 4 sulfur cluster binding Source: EcoCyc
    3. electron carrier activity Source: InterPro
    4. molybdenum ion binding Source: InterPro
    5. NADH dehydrogenase (ubiquinone) activity Source: RefGenome
    6. protein binding Source: IntAct
    7. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. aerobic respiration Source: EcoCyc
    2. ATP synthesis coupled electron transport Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Enzyme and pathway databases

    BioCyciEcoCyc:NUOG-MONOMER.
    ECOL316407:JW2278-MONOMER.
    MetaCyc:NUOG-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit G (EC:1.6.99.5)
    Alternative name(s):
    NADH dehydrogenase I subunit G
    NDH-1 subunit G
    NUO7
    Gene namesi
    Name:nuoG
    Ordered Locus Names:b2283, JW2278
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12087. nuoG.

    Subcellular locationi

    Cytoplasm 1 Publication. Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. NADH dehydrogenase complex Source: EcoliWiki
    2. plasma membrane Source: EcoliWiki
    3. plasma membrane respiratory chain complex I Source: EcoCyc

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 908907NADH-quinone oxidoreductase subunit GPRO_0000118546Add
    BLAST

    Keywords - PTMi

    Quinone

    Proteomic databases

    PaxDbiP33602.
    PRIDEiP33602.

    Expressioni

    Gene expression databases

    GenevestigatoriP33602.

    Interactioni

    Subunit structurei

    Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    nuoCP335992EBI-559737,EBI-552399
    nuoEP0AFD14EBI-559737,EBI-1117136

    Protein-protein interaction databases

    BioGridi851103. 1 interaction.
    DIPiDIP-10383N.
    IntActiP33602. 31 interactions.
    MINTiMINT-1244767.
    STRINGi511145.b2283.

    Structurei

    3D structure databases

    ProteinModelPortaliP33602.
    SMRiP33602. Positions 1-234.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 83822Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini221 – 277574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 75 kDa subunit family.Curated
    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1034.
    HOGENOMiHOG000276177.
    KOiK00336.
    OMAiGYGYVNR.
    OrthoDBiEOG6CVV7G.
    PhylomeDBiP33602.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR009010. Asp_de-COase-like_dom.
    IPR012675. Beta-grasp_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    SM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF54292. SSF54292. 1 hit.
    TIGRFAMsiTIGR01973. NuoG. 1 hit.
    PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33602-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATIHVDGKE YEVNGADNLL EACLSLGLDI PYFCWHPALG SVGACRQCAV    50
    KQYQNAEDTR GRLVMSCMTP ASDGTFISID DEEAKQFRES VVEWLMTNHP 100
    HDCPVCEEGG NCHLQDMTVM TGHSFRRYRF TKRTHRNQDL GPFISHEMNR 150
    CIACYRCVRY YKDYADGTDL GVYGAHDNVY FGRPEDGTLE SEFSGNLVEI 200
    CPTGVFTDKT HSERYNRKWD MQFAPSICQQ CSIGCNISPG ERYGELRRIE 250
    NRYNGTVNHY FLCDRGRFGY GYVNLKDRPR QPVQRRGDDF ITLNAEQAMQ 300
    GAADILRQSK KVIGIGSPRA SVESNFALRE LVGEENFYTG IAHGEQERLQ 350
    LALKVLREGG IYTPALREIE SYDAVLVLGE DVTQTGARVA LAVRQAVKGK 400
    AREMAAAQKV ADWQIAAILN IGQRAKHPLF VTNVDDTRLD DIAAWTYRAP 450
    VEDQARLGFA IAHALDNSAP AVDGIEPELQ SKIDVIVQAL AGAKKPLIIS 500
    GTNAGSLEVI QAAANVAKAL KGRGADVGIT MIARSVNSMG LGIMGGGSLE 550
    EALTELETGR ADAVVVLEND LHRHASAIRV NAALAKAPLV MVVDHQRTAI 600
    MENAHLVLSA ASFAESDGTV INNEGRAQRF FQVYDPAYYD SKTVMLESWR 650
    WLHSLHSTLL SREVDWTQLD HVIDAVVAKI PELAGIKDAA PDATFRIRGQ 700
    KLAREPHRYS GRTAMRANIS VHEPRQPQDI DTMFTFSMEG NNQPTAHRSQ 750
    VPFAWAPGWN SPQAWNKFQD EVGGKLRFGD PGVRLFETSE NGLDYFTSVP 800
    ARFQPQDGKW RIAPYYHLFG SDELSQRAPV FQSRMPQPYI KLNPADAAKL 850
    GVNAGTRVSF SYDGNTVTLP VEIAEGLTAG QVGLPMGMSG IAPVLAGAHL 900
    EDLKEAQQ 908
    Length:908
    Mass (Da):100,299
    Last modified:January 23, 2007 - v4
    Checksum:i7B8626E19709D540
    GO

    Sequence cautioni

    The sequence CAA48366.1 differs from that shown. Reason: Frameshift at positions 715 and 806.
    The sequence BAA16111.2 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti188 – 1881T → Q in CAA48366. (PubMed:7690854)Curated
    Sequence conflicti210 – 2101T → K in CAA48366. (PubMed:7690854)Curated
    Sequence conflicti390 – 3901Missing in CAA48366. (PubMed:7690854)Curated
    Sequence conflicti648 – 6481S → T in CAA48366. (PubMed:7690854)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68301 Genomic DNA. Translation: CAA48366.1. Frameshift.
    U00096 Genomic DNA. Translation: AAC75343.2.
    AP009048 Genomic DNA. Translation: BAA16111.2. Different initiation.
    L25055 Unassigned DNA. Translation: AAA03538.1.
    PIRiA65000.
    RefSeqiNP_416786.4. NC_000913.3.
    YP_490523.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75343; AAC75343; b2283.
    BAA16111; BAA16111; BAA16111.
    GeneIDi12931518.
    946762.
    KEGGiecj:Y75_p2247.
    eco:b2283.
    PATRICi32119933. VBIEscCol129921_2376.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68301 Genomic DNA. Translation: CAA48366.1 . Frameshift.
    U00096 Genomic DNA. Translation: AAC75343.2 .
    AP009048 Genomic DNA. Translation: BAA16111.2 . Different initiation.
    L25055 Unassigned DNA. Translation: AAA03538.1 .
    PIRi A65000.
    RefSeqi NP_416786.4. NC_000913.3.
    YP_490523.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P33602.
    SMRi P33602. Positions 1-234.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 851103. 1 interaction.
    DIPi DIP-10383N.
    IntActi P33602. 31 interactions.
    MINTi MINT-1244767.
    STRINGi 511145.b2283.

    Protein family/group databases

    TCDBi 3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Proteomic databases

    PaxDbi P33602.
    PRIDEi P33602.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75343 ; AAC75343 ; b2283 .
    BAA16111 ; BAA16111 ; BAA16111 .
    GeneIDi 12931518.
    946762.
    KEGGi ecj:Y75_p2247.
    eco:b2283.
    PATRICi 32119933. VBIEscCol129921_2376.

    Organism-specific databases

    EchoBASEi EB2011.
    EcoGenei EG12087. nuoG.

    Phylogenomic databases

    eggNOGi COG1034.
    HOGENOMi HOG000276177.
    KOi K00336.
    OMAi GYGYVNR.
    OrthoDBi EOG6CVV7G.
    PhylomeDBi P33602.

    Enzyme and pathway databases

    BioCyci EcoCyc:NUOG-MONOMER.
    ECOL316407:JW2278-MONOMER.
    MetaCyc:NUOG-MONOMER.

    Miscellaneous databases

    PROi P33602.

    Gene expression databases

    Genevestigatori P33602.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR001041. 2Fe-2S_ferredoxin-type.
    IPR009010. Asp_de-COase-like_dom.
    IPR012675. Beta-grasp_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    SM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF54292. SSF54292. 1 hit.
    TIGRFAMsi TIGR01973. NuoG. 1 hit.
    PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I."
      Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H.
      J. Mol. Biol. 233:109-122(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / AN387.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Mutations in NADH:ubiquinone oxidoreductase of Escherichia coli affect growth on mixed amino acids."
      Pruss B.M., Nelms J.M., Park C., Wolfe A.J.
      J. Bacteriol. 176:2143-2150(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-170.
    6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
      Link A.J., Robison K., Church G.M.
      Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-13.
      Strain: K12 / EMG2.
    7. Cited for: SUBCELLULAR LOCATION.
      Strain: BL21-DE3.

    Entry informationi

    Entry nameiNUOG_ECOLI
    AccessioniPrimary (citable) accession number: P33602
    Secondary accession number(s): P76489, P78184, P78185
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 144 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3