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P33602

- NUOG_ECOLI

UniProt

P33602 - NUOG_ECOLI

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Protein

NADH-quinone oxidoreductase subunit G

Gene

nuoG

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + quinone = NAD+ + quinol.

Cofactori

Binds 1 2Fe-2S cluster per subunit.By similarity
Binds 3 4Fe-4S clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi45 – 451Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi48 – 481Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi67 – 671Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi99 – 991Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
Metal bindingi103 – 1031Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi106 – 1061Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi112 – 1121Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi151 – 1511Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi154 – 1541Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi157 – 1571Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi201 – 2011Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi228 – 2281Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi231 – 2311Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi235 – 2351Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi263 – 2631Iron-sulfur 4 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: EcoCyc
  2. 4 iron, 4 sulfur cluster binding Source: EcoCyc
  3. electron carrier activity Source: InterPro
  4. molybdenum ion binding Source: InterPro
  5. NADH dehydrogenase (ubiquinone) activity Source: RefGenome
  6. quinone binding Source: UniProtKB-KW

GO - Biological processi

  1. aerobic respiration Source: EcoCyc
  2. ATP synthesis coupled electron transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOG-MONOMER.
ECOL316407:JW2278-MONOMER.
MetaCyc:NUOG-MONOMER.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit G (EC:1.6.99.5)
Alternative name(s):
NADH dehydrogenase I subunit G
NDH-1 subunit G
NUO7
Gene namesi
Name:nuoG
Ordered Locus Names:b2283, JW2278
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12087. nuoG.

Subcellular locationi

Cytoplasm 1 Publication. Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. NADH dehydrogenase complex Source: EcoliWiki
  2. plasma membrane Source: EcoliWiki
  3. plasma membrane respiratory chain complex I Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 908907NADH-quinone oxidoreductase subunit GPRO_0000118546Add
BLAST

Keywords - PTMi

Quinone

Proteomic databases

PaxDbiP33602.
PRIDEiP33602.

Expressioni

Gene expression databases

GenevestigatoriP33602.

Interactioni

Subunit structurei

Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
nuoCP335992EBI-559737,EBI-552399
nuoEP0AFD14EBI-559737,EBI-1117136

Protein-protein interaction databases

BioGridi851103. 1 interaction.
DIPiDIP-10383N.
IntActiP33602. 31 interactions.
MINTiMINT-1244767.
STRINGi511145.b2283.

Structurei

3D structure databases

ProteinModelPortaliP33602.
SMRiP33602. Positions 1-234.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 83822Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini221 – 277574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1034.
HOGENOMiHOG000276177.
InParanoidiP33602.
KOiK00336.
OMAiGYGYVNR.
OrthoDBiEOG6CVV7G.
PhylomeDBiP33602.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR009010. Asp_de-COase-like_dom.
IPR012675. Beta-grasp_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33602-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATIHVDGKE YEVNGADNLL EACLSLGLDI PYFCWHPALG SVGACRQCAV
60 70 80 90 100
KQYQNAEDTR GRLVMSCMTP ASDGTFISID DEEAKQFRES VVEWLMTNHP
110 120 130 140 150
HDCPVCEEGG NCHLQDMTVM TGHSFRRYRF TKRTHRNQDL GPFISHEMNR
160 170 180 190 200
CIACYRCVRY YKDYADGTDL GVYGAHDNVY FGRPEDGTLE SEFSGNLVEI
210 220 230 240 250
CPTGVFTDKT HSERYNRKWD MQFAPSICQQ CSIGCNISPG ERYGELRRIE
260 270 280 290 300
NRYNGTVNHY FLCDRGRFGY GYVNLKDRPR QPVQRRGDDF ITLNAEQAMQ
310 320 330 340 350
GAADILRQSK KVIGIGSPRA SVESNFALRE LVGEENFYTG IAHGEQERLQ
360 370 380 390 400
LALKVLREGG IYTPALREIE SYDAVLVLGE DVTQTGARVA LAVRQAVKGK
410 420 430 440 450
AREMAAAQKV ADWQIAAILN IGQRAKHPLF VTNVDDTRLD DIAAWTYRAP
460 470 480 490 500
VEDQARLGFA IAHALDNSAP AVDGIEPELQ SKIDVIVQAL AGAKKPLIIS
510 520 530 540 550
GTNAGSLEVI QAAANVAKAL KGRGADVGIT MIARSVNSMG LGIMGGGSLE
560 570 580 590 600
EALTELETGR ADAVVVLEND LHRHASAIRV NAALAKAPLV MVVDHQRTAI
610 620 630 640 650
MENAHLVLSA ASFAESDGTV INNEGRAQRF FQVYDPAYYD SKTVMLESWR
660 670 680 690 700
WLHSLHSTLL SREVDWTQLD HVIDAVVAKI PELAGIKDAA PDATFRIRGQ
710 720 730 740 750
KLAREPHRYS GRTAMRANIS VHEPRQPQDI DTMFTFSMEG NNQPTAHRSQ
760 770 780 790 800
VPFAWAPGWN SPQAWNKFQD EVGGKLRFGD PGVRLFETSE NGLDYFTSVP
810 820 830 840 850
ARFQPQDGKW RIAPYYHLFG SDELSQRAPV FQSRMPQPYI KLNPADAAKL
860 870 880 890 900
GVNAGTRVSF SYDGNTVTLP VEIAEGLTAG QVGLPMGMSG IAPVLAGAHL

EDLKEAQQ
Length:908
Mass (Da):100,299
Last modified:January 23, 2007 - v4
Checksum:i7B8626E19709D540
GO

Sequence cautioni

The sequence CAA48366.1 differs from that shown. Reason: Frameshift at positions 715 and 806.
The sequence BAA16111.2 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti188 – 1881T → Q in CAA48366. (PubMed:7690854)Curated
Sequence conflicti210 – 2101T → K in CAA48366. (PubMed:7690854)Curated
Sequence conflicti390 – 3901Missing in CAA48366. (PubMed:7690854)Curated
Sequence conflicti648 – 6481S → T in CAA48366. (PubMed:7690854)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68301 Genomic DNA. Translation: CAA48366.1. Frameshift.
U00096 Genomic DNA. Translation: AAC75343.2.
AP009048 Genomic DNA. Translation: BAA16111.2. Different initiation.
L25055 Unassigned DNA. Translation: AAA03538.1.
PIRiA65000.
RefSeqiNP_416786.4. NC_000913.3.
YP_490523.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75343; AAC75343; b2283.
BAA16111; BAA16111; BAA16111.
GeneIDi12931518.
946762.
KEGGiecj:Y75_p2247.
eco:b2283.
PATRICi32119933. VBIEscCol129921_2376.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68301 Genomic DNA. Translation: CAA48366.1 . Frameshift.
U00096 Genomic DNA. Translation: AAC75343.2 .
AP009048 Genomic DNA. Translation: BAA16111.2 . Different initiation.
L25055 Unassigned DNA. Translation: AAA03538.1 .
PIRi A65000.
RefSeqi NP_416786.4. NC_000913.3.
YP_490523.1. NC_007779.1.

3D structure databases

ProteinModelPortali P33602.
SMRi P33602. Positions 1-234.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 851103. 1 interaction.
DIPi DIP-10383N.
IntActi P33602. 31 interactions.
MINTi MINT-1244767.
STRINGi 511145.b2283.

Protein family/group databases

TCDBi 3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbi P33602.
PRIDEi P33602.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75343 ; AAC75343 ; b2283 .
BAA16111 ; BAA16111 ; BAA16111 .
GeneIDi 12931518.
946762.
KEGGi ecj:Y75_p2247.
eco:b2283.
PATRICi 32119933. VBIEscCol129921_2376.

Organism-specific databases

EchoBASEi EB2011.
EcoGenei EG12087. nuoG.

Phylogenomic databases

eggNOGi COG1034.
HOGENOMi HOG000276177.
InParanoidi P33602.
KOi K00336.
OMAi GYGYVNR.
OrthoDBi EOG6CVV7G.
PhylomeDBi P33602.

Enzyme and pathway databases

BioCyci EcoCyc:NUOG-MONOMER.
ECOL316407:JW2278-MONOMER.
MetaCyc:NUOG-MONOMER.

Miscellaneous databases

PROi P33602.

Gene expression databases

Genevestigatori P33602.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR009010. Asp_de-COase-like_dom.
IPR012675. Beta-grasp_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR01973. NuoG. 1 hit.
PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I."
    Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H.
    J. Mol. Biol. 233:109-122(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / AN387.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Mutations in NADH:ubiquinone oxidoreductase of Escherichia coli affect growth on mixed amino acids."
    Pruss B.M., Nelms J.M., Park C., Wolfe A.J.
    J. Bacteriol. 176:2143-2150(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-170.
  6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-13.
    Strain: K12 / EMG2.
  7. Cited for: SUBCELLULAR LOCATION.
    Strain: BL21-DE3.

Entry informationi

Entry nameiNUOG_ECOLI
AccessioniPrimary (citable) accession number: P33602
Secondary accession number(s): P76489, P78184, P78185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 145 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3