Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADH-quinone oxidoreductase subunit C/D

Gene

nuoC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.UniRule annotation

GO - Molecular functioni

  • NAD binding Source: InterPro
  • NADH dehydrogenase (ubiquinone) activity Source: EcoCyc
  • quinone binding Source: UniProtKB-KW

Keywordsi

Molecular functionMultifunctional enzyme, Oxidoreductase
Biological processTransport
LigandNAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOC-MONOMER
MetaCyc:NUOC-MONOMER

Protein family/group databases

TCDBi3.D.1.1.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit C/DUniRule annotation (EC:1.6.5.11UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit C/DUniRule annotation
NDH-1 subunit C/DUniRule annotation
NUO3/NUO4
Gene namesi
Name:nuoCUniRule annotation
Synonyms:nuoCDUniRule annotation, nuoDUniRule annotation
Ordered Locus Names:b2286, JW5375
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12084 nuoC

Subcellular locationi

GO - Cellular componenti

  • membrane Source: UniProtKB
  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoliWiki
  • plasma membrane respiratory chain complex I Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001186782 – 596NADH-quinone oxidoreductase subunit C/DAdd BLAST595

Keywords - PTMi

Quinone

Proteomic databases

EPDiP33599
PaxDbiP33599
PRIDEiP33599

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
nuoGP336022EBI-552399,EBI-559737

Protein-protein interaction databases

BioGridi4262976, 70 interactors
DIPiDIP-10380N
IntActiP33599, 6 interactors
MINTiP33599
STRINGi316385.ECDH10B_2448

Structurei

3D structure databases

ProteinModelPortaliP33599
SMRiP33599
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 186NADH dehydrogenase I subunit CAdd BLAST185
Regioni210 – 596NADH dehydrogenase I subunit DAdd BLAST387

Sequence similaritiesi

In the N-terminal section; belongs to the complex I 30 kDa subunit family.UniRule annotation
In the C-terminal section; belongs to the complex I 49 kDa subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQV Bacteria
COG0649 LUCA
COG0852 LUCA
HOGENOMiHOG000228264
InParanoidiP33599
KOiK13378
OMAiTHPATHG
PhylomeDBiP33599

Family and domain databases

Gene3Di1.10.645.20, 1 hit
3.30.460.80, 1 hit
HAMAPiMF_01357 NDH1_NuoC, 1 hit
MF_01359 NDH1_NuoCD_1, 1 hit
MF_01358 NDH1_NuoD, 1 hit
InterProiView protein in InterPro
IPR010218 NADH_DH_suC
IPR023062 NADH_DH_suCD
IPR001135 NADH_Q_OxRdtase_suD
IPR038290 NADH_Q_OxRdtase_suD_sf
IPR037232 NADH_quin_OxRdtase_su_C/D-like
IPR001268 NADH_UbQ_OxRdtase_30kDa_su
IPR014029 NADH_UbQ_OxRdtase_49kDa_CS
IPR020396 NADH_UbQ_OxRdtase_CS
IPR022885 NDH1_su_D/H
IPR029014 NiFe-Hase_large
PfamiView protein in Pfam
PF00329 Complex1_30kDa, 1 hit
PF00346 Complex1_49kDa, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001581 NADH_UbQ_OxRdtase_30kDa_su, 1 hit
SUPFAMiSSF143243 SSF143243, 1 hit
SSF56762 SSF56762, 1 hit
TIGRFAMsiTIGR01961 NuoC_fam, 1 hit
TIGR01962 NuoD, 1 hit
PROSITEiView protein in PROSITE
PS00542 COMPLEX1_30K, 1 hit
PS00535 COMPLEX1_49K, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDLTAQEPA WQTRDHLDDP VIGELRNRFG PDAFTVQATR TGVPVVWIKR
60 70 80 90 100
EQLLEVGDFL KKLPKPYVML FDLHGMDERL RTHREGLPAA DFSVFYHLIS
110 120 130 140 150
IDRNRDIMLK VALAENDLHV PTFTKLFPNA NWYERETWDL FGITFDGHPN
160 170 180 190 200
LRRIMMPQTW KGHPLRKDYP ARATEFSPFE LTKAKQDLEM EALTFKPEEW
210 220 230 240 250
GMKRGTENED FMFLNLGPNH PSAHGAFRIV LQLDGEEIVD CVPDIGYHHR
260 270 280 290 300
GAEKMGERQS WHSYIPYTDR IEYLGGCVNE MPYVLAVEKL AGITVPDRVN
310 320 330 340 350
VIRVMLSELF RINSHLLYIS TFIQDVGAMT PVFFAFTDRQ KIYDLVEAIT
360 370 380 390 400
GFRMHPAWFR IGGVAHDLPR GWDRLLREFL DWMPKRLASY EKAALQNTIL
410 420 430 440 450
KGRSQGVAAY GAKEALEWGT TGAGLRATGI DFDVRKARPY SGYENFDFEI
460 470 480 490 500
PVGGGVSDCY TRVMLKVEEL RQSLRILEQC LNNMPEGPFK ADHPLTTPPP
510 520 530 540 550
KERTLQHIET LITHFLQVSW GPVMPANESF QMIEATKGIN SYYLTSDGST
560 570 580 590
MSYRTRVRTP SFAHLQQIPA AIRGSLVSDL IVYLGSIDFV MSDVDR
Length:596
Mass (Da):68,236
Last modified:July 22, 2008 - v3
Checksum:i5E5EB64FE2D70FDF
GO

Sequence cautioni

The sequence AAA03535 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA16115 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA48363 differs from that shown. Reason: Frameshift at position 175.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti366H → D in CAA48363 (PubMed:8157582).Curated1
Sequence conflicti409 – 412AYGA → PMAR (PubMed:7690854).Curated4
Sequence conflicti409 – 412AYGA → PMAR (PubMed:8157582).Curated4
Sequence conflicti491A → R in CAA48363 (PubMed:8157582).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA Translation: CAA48362.1 Frameshift.
X68301 Genomic DNA Translation: CAA48363.1 Frameshift.
U00096 Genomic DNA Translation: AAC75346.2
AP009048 Genomic DNA Translation: BAA16115.2 Different initiation.
L25055 Unassigned DNA Translation: AAA03535.1 Different initiation.
PIRiD65000
RefSeqiNP_416789.2, NC_000913.3
WP_001384057.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75346; AAC75346; b2286
BAA16115; BAA16115; BAA16115
GeneIDi946759
KEGGiecj:JW5375
eco:b2286
PATRICifig|511145.12.peg.2379

Similar proteinsi

Entry informationi

Entry nameiNUOCD_ECOLI
AccessioniPrimary (citable) accession number: P33599
Secondary accession number(s): P33600, P78089, P78309
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 22, 2008
Last modified: March 28, 2018
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health