Reviewed, UniProtKB/Swiss-Prot P33599 (NUOCD_ECOLI)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit C/D
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit C/D
    NDH-1 subunit C/D
    NUO3/NUO4

Gene names

Name:	nuoC
Synonyms:	nuoCD, nuoD
Ordered Locus Names:	b2286, JW5375

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. HAMAP MF_01359
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01359
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits nuoB, CD, E, F, and G constitute the peripheral sector of the complex. HAMAP MF_01359
Subcellular location	Cytoplasm. Cell inner membrane; Peripheral membrane protein. Ref.9
Sequence similarities	In the N-terminal section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.
Sequence caution	The sequence CAA48363.1 differs from that shown. Reason: Frameshift at position 175.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Cytoplasm Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 596	595	NADH-quinone oxidoreductase subunit C/D HAMAP MF_01359		PRO_0000118678
Regions
	Region	2 – 186	185	NADH dehydrogenase I subunit C HAMAP MF_01359
	Region	210 – 596	387	NADH dehydrogenase I subunit D HAMAP MF_01359
Experimental info
	Sequence conflict	366	1	H → D in CAA48363. Ref.7
	Sequence conflict	409 – 412	4	AYGA → PMAR Ref.1
	Sequence conflict	409 – 412	4	AYGA → PMAR Ref.7
	Sequence conflict	491	1	A → R in CAA48363. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P33599-1 [UniParc]. Last modified July 22, 2008. Version 3. Checksum: 5E5EB64FE2D70FDF	FASTA	596	68,236
	10 20 30 40 50 60 MTDLTAQEPA WQTRDHLDDP VIGELRNRFG PDAFTVQATR TGVPVVWIKR EQLLEVGDFL 70 80 90 100 110 120 KKLPKPYVML FDLHGMDERL RTHREGLPAA DFSVFYHLIS IDRNRDIMLK VALAENDLHV 130 140 150 160 170 180 PTFTKLFPNA NWYERETWDL FGITFDGHPN LRRIMMPQTW KGHPLRKDYP ARATEFSPFE 190 200 210 220 230 240 LTKAKQDLEM EALTFKPEEW GMKRGTENED FMFLNLGPNH PSAHGAFRIV LQLDGEEIVD 250 260 270 280 290 300 CVPDIGYHHR GAEKMGERQS WHSYIPYTDR IEYLGGCVNE MPYVLAVEKL AGITVPDRVN 310 320 330 340 350 360 VIRVMLSELF RINSHLLYIS TFIQDVGAMT PVFFAFTDRQ KIYDLVEAIT GFRMHPAWFR 370 380 390 400 410 420 IGGVAHDLPR GWDRLLREFL DWMPKRLASY EKAALQNTIL KGRSQGVAAY GAKEALEWGT 430 440 450 460 470 480 TGAGLRATGI DFDVRKARPY SGYENFDFEI PVGGGVSDCY TRVMLKVEEL RQSLRILEQC 490 500 510 520 530 540 LNNMPEGPFK ADHPLTTPPP KERTLQHIET LITHFLQVSW GPVMPANESF QMIEATKGIN 550 560 570 580 590 SYYLTSDGST MSYRTRVRTP SFAHLQQIPA AIRGSLVSDL IVYLGSIDFV MSDVDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H. J. Mol. Biol. 233:109-122(1993) [PubMed: 7690854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / AN387.
[2]	Weidner U. Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. Strain: K12 / AN387.
[3]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Interaction of purified NDH-1 from Escherichia coli with ubiquinone analogues." David P., Baumann M., Wikstroem M., Finel M. Biochim. Biophys. Acta 1553:268-278(2002) [PubMed: 11997136] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-5.
[7]	"Mutations in NADH:ubiquinone oxidoreductase of Escherichia coli affect growth on mixed amino acids." Pruss B.M., Nelms J.M., Park C., Wolfe A.J. J. Bacteriol. 176:2143-2150(1994) [PubMed: 8157582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 175-596.
[8]	"Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli." Leif H., Sled V.D., Ohnishi T., Weiss H., Friedrich T. Eur. J. Biochem. 230:538-548(1995) [PubMed: 7607227] [Abstract] Cited for: PROTEIN SEQUENCE OF 230-232, PRELIMINARY PROTEIN SEQUENCE OF N-TERMINUS.
[9]	"Protein complexes of the Escherichia coli cell envelope." Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O. J. Biol. Chem. 280:34409-34419(2005) [PubMed: 16079137] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: BL21-DE3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X68301 Genomic DNA. Translation: CAA48362.1. Frameshift. X68301 Genomic DNA. Translation: CAA48363.1. Frameshift. U00096 Genomic DNA. Translation: AAC75346.2. AP009048 Genomic DNA. Translation: BAA16115.1. Different initiation. L25055 Unassigned DNA. Translation: AAA03535.1. Different initiation.
PIR	D65000.
RefSeq	AP_002884.1. NP_416789.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:10380N.
IntAct	P33599. 5 interactions.
Protein family/group databases
TCDB	3.D.1.1.1. proton-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
GeneID	946759.
GenomeReviews	Gene locus JW5375 in contig AP009048_GR. Gene locus b2286 in contig U00096_GR.
KEGG	ecj:JW5375. eco:b2286.
Organism-specific databases
EchoBASE	EB2009.
EcoGene	EG12084. nuoC.
CMR	Search...
Phylogenomic databases
HOGENOM	P33599.
OMA	P33599. PPTWEGH.
Enzyme and pathway databases
BioCyc	EcoCyc:NUOC-MON. MetaCyc:NUOC-MON.
Family and domain databases
HAMAP	MF_01359. [Tree]
InterPro	IPR010219. NADH_DH_1_dsu. IPR010218. NADH_DH_csu. IPR001135. NADH_Q_OxRdtase_suD. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_su-49kDa_CS. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit. TIGR01962. NuoD. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. 1 hit. PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOCD_ECOLI

Accession

Primary (citable) accession number: P33599
Secondary accession number(s): P33600, P78089, P78309

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	July 22, 2008
Last modified:	June 16, 2009
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)