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Protein

NADH-quinone oxidoreductase subunit C/D

Gene

nuoC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.UniRule annotation

GO - Molecular functioni

  • NAD binding Source: InterPro
  • NADH dehydrogenase (ubiquinone) activity Source: EcoCyc
  • quinone binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOC-MONOMER.
ECOL316407:JW5375-MONOMER.
MetaCyc:NUOC-MONOMER.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit C/DUniRule annotation (EC:1.6.5.11UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit C/DUniRule annotation
NDH-1 subunit C/DUniRule annotation
NUO3/NUO4
Gene namesi
Name:nuoCUniRule annotation
Synonyms:nuoCDUniRule annotation, nuoDUniRule annotation
Ordered Locus Names:b2286, JW5375
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12084. nuoC.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: UniProtKB
  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoliWiki
  • plasma membrane respiratory chain complex I Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 596595NADH-quinone oxidoreductase subunit C/DPRO_0000118678Add
BLAST

Keywords - PTMi

Quinone

Proteomic databases

EPDiP33599.
PaxDbiP33599.
PRIDEiP33599.

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
nuoGP336022EBI-552399,EBI-559737

Protein-protein interaction databases

BioGridi4262976. 21 interactions.
DIPiDIP-10380N.
IntActiP33599. 6 interactions.
MINTiMINT-1269278.
STRINGi511145.b2286.

Structurei

3D structure databases

ProteinModelPortaliP33599.
SMRiP33599. Positions 48-170, 212-596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 186185NADH dehydrogenase I subunit CAdd
BLAST
Regioni210 – 596387NADH dehydrogenase I subunit DAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the complex I 30 kDa subunit family.UniRule annotation
In the C-terminal section; belongs to the complex I 49 kDa subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQV. Bacteria.
COG0649. LUCA.
COG0852. LUCA.
HOGENOMiHOG000228264.
InParanoidiP33599.
KOiK13378.
OMAiPVFYMFT.
PhylomeDBiP33599.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01357. NDH1_NuoC. 1 hit.
MF_01359. NDH1_NuoCD_1. 1 hit.
MF_01358. NDH1_NuoD. 1 hit.
InterProiIPR010218. NADH_DH_suC.
IPR023062. NADH_DH_suCD.
IPR001135. NADH_Q_OxRdtase_suD.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR020396. NADH_UbQ_OxRdtase_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
PF00346. Complex1_49kDa. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01961. NuoC_fam. 1 hit.
TIGR01962. NuoD. 1 hit.
PROSITEiPS00542. COMPLEX1_30K. 1 hit.
PS00535. COMPLEX1_49K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDLTAQEPA WQTRDHLDDP VIGELRNRFG PDAFTVQATR TGVPVVWIKR
60 70 80 90 100
EQLLEVGDFL KKLPKPYVML FDLHGMDERL RTHREGLPAA DFSVFYHLIS
110 120 130 140 150
IDRNRDIMLK VALAENDLHV PTFTKLFPNA NWYERETWDL FGITFDGHPN
160 170 180 190 200
LRRIMMPQTW KGHPLRKDYP ARATEFSPFE LTKAKQDLEM EALTFKPEEW
210 220 230 240 250
GMKRGTENED FMFLNLGPNH PSAHGAFRIV LQLDGEEIVD CVPDIGYHHR
260 270 280 290 300
GAEKMGERQS WHSYIPYTDR IEYLGGCVNE MPYVLAVEKL AGITVPDRVN
310 320 330 340 350
VIRVMLSELF RINSHLLYIS TFIQDVGAMT PVFFAFTDRQ KIYDLVEAIT
360 370 380 390 400
GFRMHPAWFR IGGVAHDLPR GWDRLLREFL DWMPKRLASY EKAALQNTIL
410 420 430 440 450
KGRSQGVAAY GAKEALEWGT TGAGLRATGI DFDVRKARPY SGYENFDFEI
460 470 480 490 500
PVGGGVSDCY TRVMLKVEEL RQSLRILEQC LNNMPEGPFK ADHPLTTPPP
510 520 530 540 550
KERTLQHIET LITHFLQVSW GPVMPANESF QMIEATKGIN SYYLTSDGST
560 570 580 590
MSYRTRVRTP SFAHLQQIPA AIRGSLVSDL IVYLGSIDFV MSDVDR
Length:596
Mass (Da):68,236
Last modified:July 22, 2008 - v3
Checksum:i5E5EB64FE2D70FDF
GO

Sequence cautioni

The sequence AAA03535 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA16115 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA48363 differs from that shown. Reason: Frameshift at position 175. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti366 – 3661H → D in CAA48363 (PubMed:8157582).Curated
Sequence conflicti409 – 4124AYGA → PMAR (PubMed:7690854).Curated
Sequence conflicti409 – 4124AYGA → PMAR (PubMed:8157582).Curated
Sequence conflicti491 – 4911A → R in CAA48363 (PubMed:8157582).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48362.1. Frameshift.
X68301 Genomic DNA. Translation: CAA48363.1. Frameshift.
U00096 Genomic DNA. Translation: AAC75346.2.
AP009048 Genomic DNA. Translation: BAA16115.2. Different initiation.
L25055 Unassigned DNA. Translation: AAA03535.1. Different initiation.
PIRiD65000.
RefSeqiNP_416789.2. NC_000913.3.
WP_001384057.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75346; AAC75346; b2286.
BAA16115; BAA16115; BAA16115.
GeneIDi946759.
KEGGiecj:JW5375.
eco:b2286.
PATRICi32119939. VBIEscCol129921_2379.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48362.1. Frameshift.
X68301 Genomic DNA. Translation: CAA48363.1. Frameshift.
U00096 Genomic DNA. Translation: AAC75346.2.
AP009048 Genomic DNA. Translation: BAA16115.2. Different initiation.
L25055 Unassigned DNA. Translation: AAA03535.1. Different initiation.
PIRiD65000.
RefSeqiNP_416789.2. NC_000913.3.
WP_001384057.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP33599.
SMRiP33599. Positions 48-170, 212-596.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262976. 21 interactions.
DIPiDIP-10380N.
IntActiP33599. 6 interactions.
MINTiMINT-1269278.
STRINGi511145.b2286.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

EPDiP33599.
PaxDbiP33599.
PRIDEiP33599.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75346; AAC75346; b2286.
BAA16115; BAA16115; BAA16115.
GeneIDi946759.
KEGGiecj:JW5375.
eco:b2286.
PATRICi32119939. VBIEscCol129921_2379.

Organism-specific databases

EchoBASEiEB2009.
EcoGeneiEG12084. nuoC.

Phylogenomic databases

eggNOGiENOG4105CQV. Bacteria.
COG0649. LUCA.
COG0852. LUCA.
HOGENOMiHOG000228264.
InParanoidiP33599.
KOiK13378.
OMAiPVFYMFT.
PhylomeDBiP33599.

Enzyme and pathway databases

BioCyciEcoCyc:NUOC-MONOMER.
ECOL316407:JW5375-MONOMER.
MetaCyc:NUOC-MONOMER.

Miscellaneous databases

PROiP33599.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01357. NDH1_NuoC. 1 hit.
MF_01359. NDH1_NuoCD_1. 1 hit.
MF_01358. NDH1_NuoD. 1 hit.
InterProiIPR010218. NADH_DH_suC.
IPR023062. NADH_DH_suCD.
IPR001135. NADH_Q_OxRdtase_suD.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR020396. NADH_UbQ_OxRdtase_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
PF00346. Complex1_49kDa. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01961. NuoC_fam. 1 hit.
TIGR01962. NuoD. 1 hit.
PROSITEiPS00542. COMPLEX1_30K. 1 hit.
PS00535. COMPLEX1_49K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUOCD_ECOLI
AccessioniPrimary (citable) accession number: P33599
Secondary accession number(s): P33600, P78089, P78309
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 22, 2008
Last modified: September 7, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.