NADH-quinone oxidoreductase subunit C/D - Escherichia coli (strain K12)

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P33599 (NUOCD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit C/D
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit C/D
NDH-1 subunit C/D
NUO3/NUO4

Gene names

Name:	nuoC
Synonyms:	nuoCD, nuoD
Ordered Locus Names:	b2286, JW5375

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. HAMAP MF_01359
Catalytic activity	NADH + quinone = NAD⁺ + quinol. HAMAP MF_01359
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.
Subcellular location	Cytoplasm. Cell inner membrane; Peripheral membrane protein Ref.9.
Sequence similarities	In the N-terminal section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.
Sequence caution	The sequence AAA03535.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA16115.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAA48363.1 differs from that shown. Reason: Frameshift at position 175.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Cytoplasm Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Direct protein sequencing Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	anaerobic respiration Inferred from mutant phenotype. Source: EcoliWiki
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane respiratory chain complex I Inferred from direct assay Ref.6. Source: EcoCyc
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from mutant phenotype. Source: EcoCyc oxidoreduction-driven active transmembrane transporter activity Inferred from direct assay. Source: EcoliWiki quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.6

Chain

2 – 596

595

NADH-quinone oxidoreductase subunit C/D HAMAP MF_01359

PRO_0000118678

Regions

Region

2 – 186

185

NADH dehydrogenase I subunit C HAMAP MF_01359

Region

210 – 596

387

NADH dehydrogenase I subunit D HAMAP MF_01359

Experimental info

Sequence conflict

366

H → D in CAA48363. Ref.7

Sequence conflict

409 – 412

AYGA → PMAR Ref.1

Sequence conflict

409 – 412

AYGA → PMAR Ref.7

Sequence conflict

491

A → R in CAA48363. Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P33599 [UniParc].

Last modified July 22, 2008. Version 3.
Checksum: 5E5EB64FE2D70FDF
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FASTA

596

68,236

        10         20         30         40         50         60 
MTDLTAQEPA WQTRDHLDDP VIGELRNRFG PDAFTVQATR TGVPVVWIKR EQLLEVGDFL 

        70         80         90        100        110        120 
KKLPKPYVML FDLHGMDERL RTHREGLPAA DFSVFYHLIS IDRNRDIMLK VALAENDLHV 

       130        140        150        160        170        180 
PTFTKLFPNA NWYERETWDL FGITFDGHPN LRRIMMPQTW KGHPLRKDYP ARATEFSPFE 

       190        200        210        220        230        240 
LTKAKQDLEM EALTFKPEEW GMKRGTENED FMFLNLGPNH PSAHGAFRIV LQLDGEEIVD 

       250        260        270        280        290        300 
CVPDIGYHHR GAEKMGERQS WHSYIPYTDR IEYLGGCVNE MPYVLAVEKL AGITVPDRVN 

       310        320        330        340        350        360 
VIRVMLSELF RINSHLLYIS TFIQDVGAMT PVFFAFTDRQ KIYDLVEAIT GFRMHPAWFR 

       370        380        390        400        410        420 
IGGVAHDLPR GWDRLLREFL DWMPKRLASY EKAALQNTIL KGRSQGVAAY GAKEALEWGT 

       430        440        450        460        470        480 
TGAGLRATGI DFDVRKARPY SGYENFDFEI PVGGGVSDCY TRVMLKVEEL RQSLRILEQC 

       490        500        510        520        530        540 
LNNMPEGPFK ADHPLTTPPP KERTLQHIET LITHFLQVSW GPVMPANESF QMIEATKGIN 

       550        560        570        580        590 
SYYLTSDGST MSYRTRVRTP SFAHLQQIPA AIRGSLVSDL IVYLGSIDFV MSDVDR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H. J. Mol. Biol. 233:109-122(1993) [PubMed: 7690854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / AN387.
[2]	Weidner U. Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. Strain: K12 / AN387.
[3]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Interaction of purified NDH-1 from Escherichia coli with ubiquinone analogues." David P., Baumann M., Wikstroem M., Finel M. Biochim. Biophys. Acta 1553:268-278(2002) [PubMed: 11997136] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-5.
[7]	"Mutations in NADH:ubiquinone oxidoreductase of Escherichia coli affect growth on mixed amino acids." Pruss B.M., Nelms J.M., Park C., Wolfe A.J. J. Bacteriol. 176:2143-2150(1994) [PubMed: 8157582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 175-596.
[8]	"Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli." Leif H., Sled V.D., Ohnishi T., Weiss H., Friedrich T. Eur. J. Biochem. 230:538-548(1995) [PubMed: 7607227] [Abstract] Cited for: PROTEIN SEQUENCE OF 230-232, PRELIMINARY PROTEIN SEQUENCE OF N-TERMINUS.
[9]	"Protein complexes of the Escherichia coli cell envelope." Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O. J. Biol. Chem. 280:34409-34419(2005) [PubMed: 16079137] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: BL21-DE3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68301 Genomic DNA. Translation: CAA48362.1. Frameshift. X68301 Genomic DNA. Translation: CAA48363.1. Frameshift. U00096 Genomic DNA. Translation: AAC75346.2. AP009048 Genomic DNA. Translation: BAA16115.2. Different initiation. L25055 Unassigned DNA. Translation: AAA03535.1. Different initiation.
PIR	D65000.
RefSeq	NP_416789.2. NC_000913.2.
3D structure databases
ProteinModelPortal	P33599.
SMR	P33599. Positions 21-596.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10380N.
IntAct	P33599. 5 interactions.
MINT	MINT-1269278.
Protein family/group databases
TCDB	3.D.1.1.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001963; EBESCP00000001963; EBESCG00000001612. EBESCT00000001964; EBESCP00000001964; EBESCG00000001612. EBESCT00000016928; EBESCP00000016219; EBESCG00000015987.
GeneID	946759.
GenomeReviews	Gene locus JW5375 in contig AP009048_GR. Gene locus b2286 in contig U00096_GR.
KEGG	ecj:JW5375. eco:b2286.
PATRIC	32119939. VBIEscCol129921_2379.
Organism-specific databases
EchoBASE	EB2009.
EcoGene	EG12084. nuoC.
Phylogenomic databases
eggNOG	COG0649.
GeneTree	EBGT00050000010529.
HOGENOM	HBG459705.
OMA	PHLQQIP.
PhylomeDB	P33599.
ProtClustDB	PRK11742.
Enzyme and pathway databases
BioCyc	EcoCyc:NUOC-MONOMER. MetaCyc:NUOC-MONOMER.
Gene expression databases
Genevestigator	P33599.
Family and domain databases
HAMAP	MF_01359. NDH1_NuoCD_1. [Tree]
InterPro	IPR010219. NADH_DH_1_suD. IPR010218. NADH_DH_suC. IPR023062. NADH_DH_suC/D. IPR001135. NADH_Q_OxRdtase_suD. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR020396. NADH_UbQ_OxRdtase_CS. IPR022885. NDH1_su_D/H. [Graphical view]
KO	K13378.
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. [Graphical view]
ProDom	PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit. TIGR01962. NuoD. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. 1 hit. PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOCD_ECOLI

Accession

Primary (citable) accession number: P33599
Secondary accession number(s): P33600, P78089, P78309

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 117 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents