P33595 (SGRR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional regulator sgrR

Gene names

Name:	sgrR
Synonyms:	yabN
Ordered Locus Names:	b0069, JW0068

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activates the small RNA gene sgrS under glucose-phosphate stress conditions as well as yfdZ. Represses its own transcription under both stress and non-stress conditions; this repression likely provides one measure of control over sgrR at the level of synthesis. Might act as a sensor of the intracellular accumulation of phosphoglucose by binding these molecules in its C-terminal solute-binding domain. Ref.4 Ref.5
Miscellaneous	Binds specifically to sgrS promoter in vitro. HAMAP MF_01449
Sequence similarities	Contains 1 HTH marR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 551	551	HTH-type transcriptional regulator sgrR HAMAP MF_01449		PRO_0000168522
Regions
	Domain	1 – 116	116	HTH marR-type
	DNA binding	26 – 49	24	H-T-H motif By similarity
	Region	163 – 492	330	Solute-binding Potential

Sequences

	Sequence		Length	Mass (Da)
	P33595 [UniParc]. Last modified August 29, 2003. Version 2. Checksum: 0F4FAA1AAFC4E731	FASTA	551	63,975
	10 20 30 40 50 60 MPSARLQQQF IRLWQCCEGK SQDTTLNELA ALLSCSRRHM RTLLNTMQDR GWLTWEAEVG 70 80 90 100 110 120 RGKRSRLTFL YTGLALQQQR AEDLLEQDRI DQLVQLVGDK ATVRQMLVSH LGRSFRQGRH 130 140 150 160 170 180 ILRVLYYRPL RNLLPGSALR RSETHIARQI FSSLTRINEE NGELEADIAH HWQQISPLHW 190 200 210 220 230 240 RFFLRPGVHF HHGRELEMDD VIASLKRINT LPLYSHIADI VSPTPWTLDI HLTQPDRWLP 250 260 270 280 290 300 LLLGQVPAMI LPREWETLSN FASHPIGTGP YAVIRNSTNQ LKIQAFDDFF GYRALIDEVN 310 320 330 340 350 360 VWVLPEIADE PAGGLMLKGP QGEEKEIESR LEEGCYYLLF DSRTHRGANQ QVRDWVSYVL 370 380 390 400 410 420 SPTNLVYFAE EQYQQLWFPA YGLLPRWHHA RTIKSEKPAG LESLTLTFYQ DHSEHRVIAG 430 440 450 460 470 480 IMQQILASHQ VTLKIKEIDY DQWHTGEIES DIWLNSANFT LPLDFSVFAH LCEVPLLQHC 490 500 510 520 530 540 IPIDWQADAA RWRNGEMNLA NWCQQLVASK AMVPLLHHWL IIQGQRSMRG LRMNTLGWFD 550 FKSAWFAPPD P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 356; 384 AND 409. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Involvement of a novel transcriptional activator and small RNA in post-transcriptional regulation of the glucose phosphoenolpyruvate phosphotransferase system." Vanderpool C.K., Gottesman S. Mol. Microbiol. 54:1076-1089(2004) [PubMed: 15522088] [Abstract] Cited for: FUNCTION AS A TRANSCRIPTIONAL REGULATOR.
[5]	"The novel transcription factor SgrR coordinates the response to glucose-phosphate stress." Vanderpool C.K., Gottesman S. J. Bacteriol. 189:2238-2248(2007) [PubMed: 17209026] [Abstract] Cited for: FUNCTION AS A TRANSCRIPTIONAL REGULATOR.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73180.1. AP009048 Genomic DNA. Translation: BAB96638.2.
PIR	E64728.
RefSeq	NP_414611.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P33595.
ModBase	Search...
Protein-protein interaction databases
IntAct	P33595. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003542; EBESCP00000003542; EBESCG00000002893. EBESCT00000015351; EBESCP00000014642; EBESCG00000014411.
GeneID	944788.
GenomeReviews	Gene locus JW0068 in contig AP009048_GR. Gene locus b0069 in contig U00096_GR.
KEGG	ecj:JW0068. eco:b0069.
PATRIC	32115239. VBIEscCol129921_0071.
Organism-specific databases
EchoBASE	EB2018.
EcoGene	EG12094. sgrR.
Phylogenomic databases
eggNOG	COG4533.
GeneTree	EBGT00050000010130.
HOGENOM	HBG303837.
OMA	WHTGEIE.
PhylomeDB	P33595.
ProtClustDB	PRK13626.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12094-MONOMER.
Gene expression databases
Genevestigator	P33595.
Family and domain databases
HAMAP	MF_01449. HTH-type_SgrR. [Tree]
InterPro	IPR000914. SBP_bac_5. IPR023767. Tscrpt_reg_HTH_SgrR. [Graphical view]
KO	K11925.
Pfam	PF00496. SBP_bac_5. 1 hit. [Graphical view]
PROSITE	PS01117. HTH_MARR_1. False negative. PS50995. HTH_MARR_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SGRR_ECOLI

Accession

Primary (citable) accession number: P33595
Secondary accession number(s): P75638

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	August 29, 2003
Last modified:	January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program