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Protein

Nickel import ATP-binding protein NikE

Gene

nikE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of the ABC transporter complex NikABCDE involved in nickel import. Responsible for energy coupling to the transport system.UniRule annotation

Catalytic activityi

ATP + H2O + Ni2+(Out) = ADP + phosphate + Ni2+(In).UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • nickel cation transmembrane transport Source: EcoCyc

Keywordsi

Molecular functionHydrolase
Biological processIon transport, Nickel transport, Transport
LigandATP-binding, Nickel, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:NIKE-MONOMER
MetaCyc:NIKE-MONOMER

Protein family/group databases

TCDBi3.A.1.5.3 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Nickel import ATP-binding protein NikEUniRule annotation (EC:3.6.3.24UniRule annotation)
Gene namesi
Name:nikEUniRule annotation
Ordered Locus Names:b3480, JW3445
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12079 nikE

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000926271 – 268Nickel import ATP-binding protein NikEAdd BLAST268

Proteomic databases

PaxDbiP33594
PRIDEiP33594

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (NikD and NikE), two transmembrane proteins (NikB and NikC) and a solute-binding protein (NikA).UniRule annotation

Protein-protein interaction databases

BioGridi4262508, 18 interactors
DIPiDIP-10344N
IntActiP33594, 11 interactors
STRINGi316385.ECDH10B_3654

Structurei

3D structure databases

ProteinModelPortaliP33594
SMRiP33594
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 252ABC transporterUniRule annotationAdd BLAST249

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Nickel importer (TC 3.A.1.5.3) family. [View classification]UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EFJ Bacteria
COG1124 LUCA
InParanoidiP33594
KOiK10824
OMAiANICQAI
PhylomeDBiP33594

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR015858 ABC_transpr_NikE
IPR014137 Nickel_NikE
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00005 ABC_tran, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02769 nickel_nikE, 1 hit
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit
PS51248 NIKE, 1 hit

Sequencei

Sequence statusi: Complete.

P33594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLNISGLS HHYAHGGFNG KHQHQAVLNN VSLTLKSGET VALLGRSGCG
60 70 80 90 100
KSTLARLLVG LESPAQGNIS WRGEPLAKLN RAQRKAFRRD IQMVFQDSIS
110 120 130 140 150
AVNPRKTVRE ILREPMRHLL SLKKSEQLAR ASEMLKAVDL DDSVLDKRPP
160 170 180 190 200
QLSGGQLQRV CLARALAVEP KLLILDEAVS NLDLVLQAGV IRLLKKLQQQ
210 220 230 240 250
FGTACLFITH DLRLVERFCQ RVMVMDNGQI VETQVVGEKL TFSSDAGRVL
260
QNAVLPAFPV RRRTTEKV
Length:268
Mass (Da):29,722
Last modified:August 29, 2003 - v2
Checksum:i8413CB9E8D7C585A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46 – 47RS → GT in CAA51663 (PubMed:7934931).Curated2
Sequence conflicti84R → A in CAA51663 (PubMed:7934931).Curated1
Sequence conflicti132 – 133SE → RQ in CAA51663 (PubMed:7934931).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73143 Genomic DNA Translation: CAA51663.1
U00039 Genomic DNA Translation: AAB18455.1
U00096 Genomic DNA Translation: AAC76505.1
AP009048 Genomic DNA Translation: BAE77813.1
PIRiC65145
RefSeqiNP_417937.1, NC_000913.3
WP_000173631.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76505; AAC76505; b3480
BAE77813; BAE77813; BAE77813
GeneIDi947987
KEGGiecj:JW3445
eco:b3480
PATRICifig|1411691.4.peg.3245

Similar proteinsi

Entry informationi

Entry nameiNIKE_ECOLI
AccessioniPrimary (citable) accession number: P33594
Secondary accession number(s): Q2M7E3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 29, 2003
Last modified: March 28, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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