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Protein

Nickel import ATP-binding protein NikE

Gene

nikE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the ABC transporter complex NikABCDE involved in nickel import. Responsible for energy coupling to the transport system.UniRule annotation

Catalytic activityi

ATP + H2O + Ni2+(Out) = ADP + phosphate + Ni2+(In).UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 528ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Nickel transport, Transport

Keywords - Ligandi

ATP-binding, Nickel, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:NIKE-MONOMER.
ECOL316407:JW3445-MONOMER.
MetaCyc:NIKE-MONOMER.

Protein family/group databases

TCDBi3.A.1.5.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Nickel import ATP-binding protein NikEUniRule annotation (EC:3.6.3.24UniRule annotation)
Gene namesi
Name:nikEUniRule annotation
Ordered Locus Names:b3480, JW3445
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12079. nikE.

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Nickel import ATP-binding protein NikEPRO_0000092627Add
BLAST

Proteomic databases

PaxDbiP33594.
PRIDEiP33594.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (NikD and NikE), two transmembrane proteins (NikB and NikC) and a solute-binding protein (NikA).UniRule annotation

Protein-protein interaction databases

BioGridi4262508. 18 interactions.
DIPiDIP-10344N.
IntActiP33594. 10 interactions.
MINTiMINT-1220565.
STRINGi511145.b3480.

Structurei

3D structure databases

ProteinModelPortaliP33594.
SMRiP33594. Positions 5-238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 252249ABC transporterUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Nickel importer (TC 3.A.1.5.3) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EFJ. Bacteria.
COG1124. LUCA.
InParanoidiP33594.
KOiK10824.
OMAiERIAACH.
PhylomeDBiP33594.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015858. ABC_transpr_NikE.
IPR014137. Nickel_NikE.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02769. nickel_nikE. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51248. NIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLNISGLS HHYAHGGFNG KHQHQAVLNN VSLTLKSGET VALLGRSGCG
60 70 80 90 100
KSTLARLLVG LESPAQGNIS WRGEPLAKLN RAQRKAFRRD IQMVFQDSIS
110 120 130 140 150
AVNPRKTVRE ILREPMRHLL SLKKSEQLAR ASEMLKAVDL DDSVLDKRPP
160 170 180 190 200
QLSGGQLQRV CLARALAVEP KLLILDEAVS NLDLVLQAGV IRLLKKLQQQ
210 220 230 240 250
FGTACLFITH DLRLVERFCQ RVMVMDNGQI VETQVVGEKL TFSSDAGRVL
260
QNAVLPAFPV RRRTTEKV
Length:268
Mass (Da):29,722
Last modified:August 29, 2003 - v2
Checksum:i8413CB9E8D7C585A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 472RS → GT in CAA51663 (PubMed:7934931).Curated
Sequence conflicti84 – 841R → A in CAA51663 (PubMed:7934931).Curated
Sequence conflicti132 – 1332SE → RQ in CAA51663 (PubMed:7934931).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73143 Genomic DNA. Translation: CAA51663.1.
U00039 Genomic DNA. Translation: AAB18455.1.
U00096 Genomic DNA. Translation: AAC76505.1.
AP009048 Genomic DNA. Translation: BAE77813.1.
PIRiC65145.
RefSeqiNP_417937.1. NC_000913.3.
WP_000173631.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76505; AAC76505; b3480.
BAE77813; BAE77813; BAE77813.
GeneIDi947987.
KEGGiecj:JW3445.
eco:b3480.
PATRICi32122404. VBIEscCol129921_3579.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73143 Genomic DNA. Translation: CAA51663.1.
U00039 Genomic DNA. Translation: AAB18455.1.
U00096 Genomic DNA. Translation: AAC76505.1.
AP009048 Genomic DNA. Translation: BAE77813.1.
PIRiC65145.
RefSeqiNP_417937.1. NC_000913.3.
WP_000173631.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP33594.
SMRiP33594. Positions 5-238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262508. 18 interactions.
DIPiDIP-10344N.
IntActiP33594. 10 interactions.
MINTiMINT-1220565.
STRINGi511145.b3480.

Protein family/group databases

TCDBi3.A.1.5.3. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP33594.
PRIDEiP33594.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76505; AAC76505; b3480.
BAE77813; BAE77813; BAE77813.
GeneIDi947987.
KEGGiecj:JW3445.
eco:b3480.
PATRICi32122404. VBIEscCol129921_3579.

Organism-specific databases

EchoBASEiEB2004.
EcoGeneiEG12079. nikE.

Phylogenomic databases

eggNOGiENOG4105EFJ. Bacteria.
COG1124. LUCA.
InParanoidiP33594.
KOiK10824.
OMAiERIAACH.
PhylomeDBiP33594.

Enzyme and pathway databases

BioCyciEcoCyc:NIKE-MONOMER.
ECOL316407:JW3445-MONOMER.
MetaCyc:NIKE-MONOMER.

Miscellaneous databases

PROiP33594.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015858. ABC_transpr_NikE.
IPR014137. Nickel_NikE.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02769. nickel_nikE. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51248. NIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIKE_ECOLI
AccessioniPrimary (citable) accession number: P33594
Secondary accession number(s): Q2M7E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 29, 2003
Last modified: September 7, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.