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Protein

Nickel transport system permease protein NikB

Gene

nikB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane.

GO - Molecular functioni

  • nickel cation binding Source: EcoCyc
  • nickel cation transmembrane transporter activity Source: EcoCyc

Keywordsi

Biological processIon transport, Nickel transport, Transport
LigandNickel

Enzyme and pathway databases

BioCyciEcoCyc:NIKB-MONOMER
MetaCyc:NIKB-MONOMER

Protein family/group databases

TCDBi3.A.1.5.3 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Nickel transport system permease protein NikB
Gene namesi
Name:nikB
Ordered Locus Names:b3477, JW3442
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12076 nikB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini30 – 103PeriplasmicSequence analysisAdd BLAST74
Transmembranei104 – 124HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini125 – 136CytoplasmicSequence analysisAdd BLAST12
Transmembranei137 – 157HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini158 – 169PeriplasmicSequence analysisAdd BLAST12
Transmembranei170 – 190HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini191 – 231CytoplasmicSequence analysisAdd BLAST41
Transmembranei232 – 252HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini253 – 274PeriplasmicSequence analysisAdd BLAST22
Transmembranei275 – 295HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini296 – 314CytoplasmicSequence analysisAdd BLAST19

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000601201 – 314Nickel transport system permease protein NikBAdd BLAST314

Proteomic databases

PaxDbiP33591
PRIDEiP33591

Interactioni

Subunit structurei

Probably forms a heterodimeric pore with NikC.

Protein-protein interaction databases

BioGridi4262492, 20 interactors
DIPiDIP-10341N
STRINGi316385.ECDH10B_3651

Structurei

3D structure databases

ProteinModelPortaliP33591
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 295ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST198

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJM Bacteria
COG0601 LUCA
HOGENOMiHOG000170251
InParanoidiP33591
KOiK15585
OMAiRAGRNSM
PhylomeDBiP33591

Family and domain databases

CDDicd06261 TM_PBP2, 1 hit
Gene3Di1.10.3720.10, 1 hit
InterProiView protein in InterPro
IPR000515 MetI-like
IPR035906 MetI-like_sf
IPR014156 Nickel_NikB
PfamiView protein in Pfam
PF00528 BPD_transp_1, 1 hit
SUPFAMiSSF161098 SSF161098, 1 hit
TIGRFAMsiTIGR02789 nickel_nikB, 1 hit
PROSITEiView protein in PROSITE
PS50928 ABC_TM1, 1 hit

Sequencei

Sequence statusi: Complete.

P33591-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRYVLRRFL LLIPMVLAAS VIIFLMLRLG TGDPALDYLR LSNLPPTPEM
60 70 80 90 100
LASTRTMLGL DQPLYVQYGT WLWKALHLDF GISFASQRPV LDDMLNFLPA
110 120 130 140 150
TLELAGAALV LILLTSVPLG IWAARHRDRL PDFAVRFIAF LGVSMPNFWL
160 170 180 190 200
AFLLVMAFSV YLQWLPAMGY GGWQHIILPA VSIAFMSLAI NARLLRASML
210 220 230 240 250
DVAGQRHVTW ARLRGLNDKQ TERRHILRNA SLPMITAVGM HIGELIGGTM
260 270 280 290 300
IIENIFAWPG VGRYAVSAIF NRDYPVIQCF TLMMVVVFVV CNLIVDLLNA
310
ALDPRIRRHE GAHA
Length:314
Mass (Da):35,248
Last modified:August 29, 2003 - v2
Checksum:iB1251307DBE219B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26 – 27ML → IV in CAA51660 (PubMed:7934931).Curated2
Sequence conflicti197A → P in CAA51660 (PubMed:7934931).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73143 Genomic DNA Translation: CAA51660.1
U00039 Genomic DNA Translation: AAB18452.1
U00096 Genomic DNA Translation: AAC76502.1
AP009048 Genomic DNA Translation: BAE77816.1
PIRiS47696
RefSeqiNP_417934.1, NC_000913.3
WP_000947068.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76502; AAC76502; b3477
BAE77816; BAE77816; BAE77816
GeneIDi947986
KEGGiecj:JW3442
eco:b3477
PATRICifig|1411691.4.peg.3248

Similar proteinsi

Entry informationi

Entry nameiNIKB_ECOLI
AccessioniPrimary (citable) accession number: P33591
Secondary accession number(s): Q2M7E0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 29, 2003
Last modified: March 28, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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