Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nickel transport system permease protein NikB

Gene

nikB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane.

GO - Molecular functioni

  • nickel cation binding Source: EcoCyc
  • nickel cation transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • nickel cation transmembrane transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Ion transport, Nickel transport, Transport

Keywords - Ligandi

Nickel

Enzyme and pathway databases

BioCyciEcoCyc:NIKB-MONOMER.
ECOL316407:JW3442-MONOMER.
MetaCyc:NIKB-MONOMER.

Protein family/group databases

TCDBi3.A.1.5.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Nickel transport system permease protein NikB
Gene namesi
Name:nikB
Ordered Locus Names:b3477, JW3442
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12076. nikB.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini30 – 103PeriplasmicSequence analysisAdd BLAST74
Transmembranei104 – 124HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini125 – 136CytoplasmicSequence analysisAdd BLAST12
Transmembranei137 – 157HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini158 – 169PeriplasmicSequence analysisAdd BLAST12
Transmembranei170 – 190HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini191 – 231CytoplasmicSequence analysisAdd BLAST41
Transmembranei232 – 252HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini253 – 274PeriplasmicSequence analysisAdd BLAST22
Transmembranei275 – 295HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini296 – 314CytoplasmicSequence analysisAdd BLAST19

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000601201 – 314Nickel transport system permease protein NikBAdd BLAST314

Proteomic databases

PaxDbiP33591.
PRIDEiP33591.

Interactioni

Subunit structurei

Probably forms a heterodimeric pore with NikC.

Protein-protein interaction databases

BioGridi4262492. 13 interactors.
DIPiDIP-10341N.
STRINGi511145.b3477.

Structurei

3D structure databases

ProteinModelPortaliP33591.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 295ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST198

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJM. Bacteria.
COG0601. LUCA.
HOGENOMiHOG000170251.
InParanoidiP33591.
KOiK15585.
OMAiRHEGAHS.
PhylomeDBiP33591.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
IPR014156. Nickel_NikB.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
TIGRFAMsiTIGR02789. nickel_nikB. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33591-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRYVLRRFL LLIPMVLAAS VIIFLMLRLG TGDPALDYLR LSNLPPTPEM
60 70 80 90 100
LASTRTMLGL DQPLYVQYGT WLWKALHLDF GISFASQRPV LDDMLNFLPA
110 120 130 140 150
TLELAGAALV LILLTSVPLG IWAARHRDRL PDFAVRFIAF LGVSMPNFWL
160 170 180 190 200
AFLLVMAFSV YLQWLPAMGY GGWQHIILPA VSIAFMSLAI NARLLRASML
210 220 230 240 250
DVAGQRHVTW ARLRGLNDKQ TERRHILRNA SLPMITAVGM HIGELIGGTM
260 270 280 290 300
IIENIFAWPG VGRYAVSAIF NRDYPVIQCF TLMMVVVFVV CNLIVDLLNA
310
ALDPRIRRHE GAHA
Length:314
Mass (Da):35,248
Last modified:August 29, 2003 - v2
Checksum:iB1251307DBE219B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26 – 27ML → IV in CAA51660 (PubMed:7934931).Curated2
Sequence conflicti197A → P in CAA51660 (PubMed:7934931).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73143 Genomic DNA. Translation: CAA51660.1.
U00039 Genomic DNA. Translation: AAB18452.1.
U00096 Genomic DNA. Translation: AAC76502.1.
AP009048 Genomic DNA. Translation: BAE77816.1.
PIRiS47696.
RefSeqiNP_417934.1. NC_000913.3.
WP_000947068.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76502; AAC76502; b3477.
BAE77816; BAE77816; BAE77816.
GeneIDi947986.
KEGGiecj:JW3442.
eco:b3477.
PATRICi32122398. VBIEscCol129921_3576.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73143 Genomic DNA. Translation: CAA51660.1.
U00039 Genomic DNA. Translation: AAB18452.1.
U00096 Genomic DNA. Translation: AAC76502.1.
AP009048 Genomic DNA. Translation: BAE77816.1.
PIRiS47696.
RefSeqiNP_417934.1. NC_000913.3.
WP_000947068.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP33591.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262492. 13 interactors.
DIPiDIP-10341N.
STRINGi511145.b3477.

Protein family/group databases

TCDBi3.A.1.5.3. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP33591.
PRIDEiP33591.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76502; AAC76502; b3477.
BAE77816; BAE77816; BAE77816.
GeneIDi947986.
KEGGiecj:JW3442.
eco:b3477.
PATRICi32122398. VBIEscCol129921_3576.

Organism-specific databases

EchoBASEiEB2001.
EcoGeneiEG12076. nikB.

Phylogenomic databases

eggNOGiENOG4105CJM. Bacteria.
COG0601. LUCA.
HOGENOMiHOG000170251.
InParanoidiP33591.
KOiK15585.
OMAiRHEGAHS.
PhylomeDBiP33591.

Enzyme and pathway databases

BioCyciEcoCyc:NIKB-MONOMER.
ECOL316407:JW3442-MONOMER.
MetaCyc:NIKB-MONOMER.

Miscellaneous databases

PROiP33591.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
IPR014156. Nickel_NikB.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
TIGRFAMsiTIGR02789. nickel_nikB. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIKB_ECOLI
AccessioniPrimary (citable) accession number: P33591
Secondary accession number(s): Q2M7E0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 29, 2003
Last modified: November 2, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.