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P33590 (NIKA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nickel-binding periplasmic protein
Gene names
Name:nikA
Ordered Locus Names:b3476, JW3441
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in a nickel transport system, probably represents the nickel binder.

Subcellular location

Periplasm Probable.

Sequence similarities

Belongs to the bacterial solute-binding protein 5 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222
Chain23 – 524502Nickel-binding periplasmic protein
PRO_0000031800

Secondary structure

............................................................................................. 524
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P33590 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: CB2E3C1CDCE42396

FASTA52458,719
        10         20         30         40         50         60 
MLSTLRRTLF ALLACASFIV HAAAPDEITT AWPVNVGPLN PHLYTPNQMF AQSMVYEPLV 

        70         80         90        100        110        120 
KYQADGSVIP WLAKSWTHSE DGKTWTFTLR DDVKFSNGEP FDAEAAAENF RAVLDNRQRH 

       130        140        150        160        170        180 
AWLELANQIV DVKALSKTEL QITLKSAYYP FLQELALPRP FRFIAPSQFK NHETMNGIKA 

       190        200        210        220        230        240 
PIGTGPWILQ ESKLNQYDVF VRNENYWGEK PAIKKITFNV IPDPTTRAVA FETGDIDLLY 

       250        260        270        280        290        300 
GNEGLLPLDT FARFSQNPAY HTQLSQPIET VMLALNTAKA PTNELAVREA LNYAVNKKSL 

       310        320        330        340        350        360 
IDNALYGTQQ VADTLFAPSV PYANLGLKPS QYDPQKAKAL LEKAGWTLPA GKDIREKNGQ 

       370        380        390        400        410        420 
PLRIELSFIG TDALSKSMAE IIQADMRQIG ADVSLIGEEE SSIYARQRDG RFGMIFHRTW 

       430        440        450        460        470        480 
GAPYDPHAFL SSMRVPSHAD FQAQQGLADK PLIDKEIGEV LATHDETQRQ ALYRDILTRL 

       490        500        510        520 
HDEAVYLPIS YISMMVVSKP ELGNIPYAPI ATEIPFEQIK PVKP 

« Hide

References

« Hide 'large scale' references
[1]"The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system for nickel."
Navarro C., Wu L.-F., Mandrand-Berthelot M.-A.
Mol. Microbiol. 9:1181-1191(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Crystal structures of the liganded and unliganded nickel-binding protein NikA from Escherichia coli."
Heddle J., Scott D.J., Unzai S., Park S.Y., Tame J.R.
J. Biol. Chem. 278:50322-50329(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 23-524.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X73143 Genomic DNA. Translation: CAA51659.1.
U00039 Genomic DNA. Translation: AAB18451.1.
U00096 Genomic DNA. Translation: AAC76501.1.
AP009048 Genomic DNA. Translation: BAE77817.1.
PIRS39594.
RefSeqNP_417933.1. NC_000913.3.
YP_491958.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UIUX-ray1.85A/B23-524[»]
1UIVX-ray1.95A/B23-524[»]
1ZLQX-ray1.80A/B23-524[»]
2NOOX-ray1.65A23-524[»]
3DP8X-ray2.50A/B/C23-524[»]
3E3KX-ray2.80A/B/C23-524[»]
3MVWX-ray1.79A/B23-524[»]
3MVXX-ray1.70A/B23-524[»]
3MVYX-ray2.50A/B23-524[»]
3MVZX-ray1.70A/B23-524[»]
3MW0X-ray2.30A/B23-524[»]
3MZ9X-ray1.80A/B23-524[»]
3MZBX-ray1.70A/B23-523[»]
3QIMX-ray2.10A/B23-524[»]
4DCXX-ray2.00A/B23-524[»]
4DCYX-ray2.00A/B23-524[»]
4I8CX-ray2.50A/B/C23-524[»]
4I9DX-ray1.70A/B23-524[»]
ProteinModelPortalP33590.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10340N.
IntActP33590. 1 interaction.
MINTMINT-1272849.
STRING511145.b3476.

Protein family/group databases

TCDB3.A.1.5.3. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbP33590.
PRIDEP33590.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76501; AAC76501; b3476.
BAE77817; BAE77817; BAE77817.
GeneID12933497.
947981.
KEGGecj:Y75_p3702.
eco:b3476.
PATRIC32122396. VBIEscCol129921_3575.

Organism-specific databases

EchoBASEEB2000.
EcoGeneEG12075. nikA.

Phylogenomic databases

eggNOGCOG0747.
HOGENOMHOG000125740.
KOK15584.
OMAGKNQINI.
OrthoDBEOG6KHFZ2.
PhylomeDBP33590.

Enzyme and pathway databases

BioCycEcoCyc:NIKA-MONOMER.
ECOL316407:JW3441-MONOMER.
MetaCyc:NIKA-MONOMER.

Gene expression databases

GenevestigatorP33590.

Family and domain databases

InterProIPR011980. NikA_ABC_Ni-bd.
IPR000914. SBP_bac_5.
IPR023765. SBP_bac_5_CS.
[Graphical view]
PfamPF00496. SBP_bac_5. 1 hit.
[Graphical view]
TIGRFAMsTIGR02294. nickel_nikA. 1 hit.
PROSITEPS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP33590.
PROP33590.

Entry information

Entry nameNIKA_ECOLI
AccessionPrimary (citable) accession number: P33590
Secondary accession number(s): Q2M7D9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene