Reviewed, UniProtKB/Swiss-Prot P33589 (VSPA_LACMU)

Names and origin

Protein names	Recommended name:     Gyroxin analog     EC=3.4.21.74 Alternative name(s):     Thrombin-like enzyme     LM-TL     Venombin-A
Organism	Lachesis muta muta (Bushmaster)
Taxonomic identifier	8753 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Crotalinae › Lachesis

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Thrombin-like snake venom serine protease. Displays a specificity similar to trypsin. Releases only fibrinopeptide A in the conversion of fibrinogen to fibrin.
Catalytic activity	Selective cleavage of Arg-|-Xaa bond in fibrinogen, to form fibrin, and release fibrinopeptide A. The specificity of further degradation of fibrinogen varies with species origin of the enzyme.
Enzyme regulation	Inhibited competitively by amidines and guanidines, and irreversibly inhibited by diisopropylfluorophosphate.
Subunit structure	Monomer. Ref.2
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the peptidase S1 family. Snake venom subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Blood coagulation
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease Toxin
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Gyroxin analog		PRO_0000088740
Regions
	Domain	1 – 222	222	Peptidase S1
Sites
	Active site	43	1	Charge relay system
	Active site	88	1	Charge relay system
	Active site	177	1	Charge relay system
Amino acid modifications
	Glycosylation	45	1	N-linked (GlcNAc...)
	Glycosylation	81	1	N-linked (GlcNAc...)
	Glycosylation	145	1	N-linked (GlcNAc...)
	Glycosylation	224	1	N-linked (GlcNAc...)
	Disulfide bond	7 ↔ 138		By similarity
	Disulfide bond	28 ↔ 44		By similarity
	Disulfide bond	78 ↔ 227		By similarity
	Disulfide bond	117 ↔ 183		By similarity
	Disulfide bond	149 ↔ 162		By similarity
	Disulfide bond	173 ↔ 198		By similarity
Experimental info
	Sequence conflict	102	1	E → A AA sequence Ref.2
	Sequence conflict	109	1	N → S AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P33589-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: DA52305D2995C52D	FASTA	228	25,629
	10 20 30 40 50 60 VIGGDECNIN EHRFLVALYD GLSGTFLCGG TLINQEWVLT AQHCNRSLMN IYLGMHNKNV 70 80 90 100 110 120 KFDDEQRRYP KKKYFFRCNK NFTKWDEDIR LNRPVRFSAH IEPLSLPSNP PSEDSVCRVM 130 140 150 160 170 180 GWGQITSPPE TLPDVPHCAN INLFNYTVCR GAYPRMPTKV LCAGVLEGGI DTCNRDSGGP 190 200 210 220 LICNGQFQGI VFWGPDPCAQ PDKPGVYTKV FDYLDWIQSV IAGNTTCS

References

[1]	"The complete amino acid sequence of a thrombin-like enzyme/gyroxin analogue from venom of the bushmaster snake (Lachesis muta muta)." Magalhaes A., Campos-Brasil da Fonseca B., Ribeiro Diniz C., Gilroy J., Richarson M. FEBS Lett. 329:116-120(1993) [PubMed: 8354384] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[2]	"Structural features of a snake venom thrombin-like enzyme: thrombin and trypsin on a single catalytic platform?" Castro H.C., Silva D.M., Craik C., Zingali R.B. Biochim. Biophys. Acta 1547:183-195(2001) [PubMed: 11410274] [Abstract] Cited for: PROTEIN SEQUENCE, SUBUNIT, 3D-STRUCTURE MODELING.
[3]	"A gyroxin analog from the venom of the bushmaster (Lachesis muta muta)." da Silva N.J., Aird S.D., Seebart C., Kaiser I.I. Toxicon 27:763-771(1989) [PubMed: 2781576] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-25. Tissue: Venom.

Cross-references

Sequence databases
PIR	S35689.
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Protein family/group databases
MEROPS	S01.432.
Phylogenomic databases
HOVERGEN	P33589.
Enzyme and pathway databases
BRENDA	3.4.21.74. 278404.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. False negative. PS00135. TRYPSIN_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VSPA_LACMU

Accession

Primary (citable) accession number: P33589

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)