Reviewed, UniProtKB/Swiss-Prot P33574 (PLMN_PETMA)

Names and origin

Protein names	Recommended name:     Plasminogen     EC=3.4.21.7
Organism	Petromyzon marinus (Sea lamprey)
Taxonomic identifier	7757 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Hyperoartia › Petromyzontiformes › Petromyzontidae › Petromyzon

Protein attributes

Sequence length	325 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation.
Catalytic activity	Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa; higher selectivity than trypsin. Converts fibrin into soluble products.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1 family. Plasminogen subfamily. Contains at least 2 kringle domains.

Ontologies

Keywords
Biological process	Blood coagulation Fibrinolysis Tissue remodeling
Cellular component	Secreted
Domain	Kringle
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW fibrinolysis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro tissue remodeling Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›325	›325	Plasminogen		PRO_0000088706
Amino acid modifications
	Disulfide bond	81 ↔ 146		By similarity
	Disulfide bond	102 ↔ 135		By similarity
	Disulfide bond	124 ↔ 141		By similarity
	Disulfide bond	188 ↔ 212		By similarity
Experimental info
	Non-adjacent residues	15 – 16	2
	Non-adjacent residues	34 – 35	2
	Non-adjacent residues	44 – 45	2
	Non-adjacent residues	76 – 77	2
	Non-adjacent residues	111 – 112	2
	Non-adjacent residues	138 – 139	2
	Non-adjacent residues	158 – 159	2
	Non-adjacent residues	178 – 179	2
	Non-adjacent residues	216 – 217	2
	Non-adjacent residues	236 – 237	2
	Non-adjacent residues	267 – 268	2
	Non-adjacent residues	282 – 283	2
	Non-adjacent residues	295 – 296	2
	Non-adjacent residues	307 – 308	2
	Non-adjacent residues	315 – 316	2
	Non-terminal residue	325	1

Sequences

	Sequence		Length	Mass (Da)
	P33574-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 1B5F0B539AC6ED3C	FASTA	325	35,206
	10 20 30 40 50 60 APIKGYSVTV XLYIFDCQKW SSNYPHKPNF SDATDPKGPW CYTTDYXGAA SVTRSGLRGD 70 80 90 100 110 120 EQTPHRHTFS PQSFAGLTTA CVKGTGEGYR GTAALTVSGK ACQAWASQTP GDVYSCQGLV 130 140 150 160 170 180 SNYCRNPDGE KLPWCYTTEY CNVPSCTGGP TGSEYHEILT PAQDXYTGIV EDYRGKMSPD 190 200 210 220 230 240 AGLEENFCRN PDQDPQGPWC YTXNPEAXPR YCDVLSVVGG XEAQRNSXPR QISLQYGWQT 250 260 270 280 290 300 VHVQSIHAEP RGVDIALVKI APPAQLTACV ITXWGETQGT GEDVQFCAGY PEGGTDPVSL 310 320 VCLEPCVLAP VLVGVVILPX NDRXQ

References

[1]	"Isolation, characterization and partial amino acid sequence of lamprey plasminogen." Affolter M., Schaller J., Rickli E.E. Protein Seq. Data Anal. 5:207-211(1993) Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
PIR	S33879.
3D structure databases
HSSP	HSSP built from PDB template 5HPG based on UniProtKB P00747.
ModBase	Search...
Protein family/group databases
MEROPS	S01.233.
Phylogenomic databases
HOVERGEN	P33574.
Enzyme and pathway databases
BRENDA	3.4.21.7. 276373.
Family and domain databases
InterPro	IPR000001. Kringle. IPR018056. Kringle_CS. IPR018059. Kringle_sub. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. [Graphical view]
Gene3D	G3DSA:2.40.20.10. Kringle. 2 hits.
Pfam	PF00051. Kringle. 2 hits. [Graphical view]
PRINTS	PR00018. KRINGLE.
ProDom	PD000395. Kringle. 3 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00130. KR. 2 hits. SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS00021. KRINGLE_1. 2 hits. PS50070. KRINGLE_2. 2 hits. PS50240. TRYPSIN_DOM. Partial match. PS00134. TRYPSIN_HIS. Partial match. PS00135. TRYPSIN_SER. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLMN_PETMA

Accession

Primary (citable) accession number: P33574

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)