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Protein

Transketolase 2

Gene

tktB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate. Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate (By similarity).By similarity

Catalytic activityi

Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity, Ca2+By similarity, Mn2+By similarity, Co2+By similarityNote: Binds 1 Mg2+ ion per subunit. Can also utilize other divalent metal cations, such as Ca2+, Mn2+ and Co2+.By similarity
  • thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei25SubstrateBy similarity1
Sitei25Important for catalytic activityBy similarity1
Binding sitei65Thiamine pyrophosphateBy similarity1
Metal bindingi154MagnesiumBy similarity1
Binding sitei155Thiamine pyrophosphate; via amide nitrogenBy similarity1
Metal bindingi184MagnesiumBy similarity1
Binding sitei184Thiamine pyrophosphateBy similarity1
Metal bindingi186Magnesium; via carbonyl oxygenBy similarity1
Binding sitei260SubstrateBy similarity1
Binding sitei260Thiamine pyrophosphateBy similarity1
Sitei260Important for catalytic activityBy similarity1
Binding sitei357SubstrateBy similarity1
Binding sitei384SubstrateBy similarity1
Active sitei410Proton donorBy similarity1
Binding sitei436Thiamine pyrophosphateBy similarity1
Binding sitei460SubstrateBy similarity1
Binding sitei468SubstrateBy similarity1
Binding sitei519SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi113 – 115Thiamine pyrophosphateBy similarity3

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • transketolase activity Source: EcoliWiki

GO - Biological processi

Keywordsi

Molecular functionTransferase
LigandCalcium, Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciEcoCyc:TRANSKETOII-MONOMER
MetaCyc:TRANSKETOII-MONOMER
BRENDAi2.2.1.1 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Transketolase 2 (EC:2.2.1.1)
Short name:
TK 2
Gene namesi
Name:tktB
Ordered Locus Names:b2465, JW2449
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12100 tktB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001918571 – 667Transketolase 2Add BLAST667

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei342N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP33570
PaxDbiP33570
PRIDEiP33570

PTM databases

iPTMnetiP33570

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4262216, 19 interactors
DIPiDIP-10999N
IntActiP33570, 6 interactors
STRINGi316385.ECDH10B_2630

Structurei

3D structure databases

ProteinModelPortaliP33570
SMRiP33570
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transketolase family.Curated

Phylogenomic databases

eggNOGiENOG4105CV1 Bacteria
COG0021 LUCA
HOGENOMiHOG000225953
InParanoidiP33570
KOiK00615
OMAiYALQQTD
PhylomeDBiP33570

Family and domain databases

Gene3Di3.40.50.920, 1 hit
InterProiView protein in InterPro
IPR029061 THDP-binding
IPR009014 Transketo_C/PFOR_II
IPR005475 Transketolase-like_Pyr-bd
IPR005478 Transketolase_bac-like
IPR020826 Transketolase_BS
IPR033248 Transketolase_C
IPR033247 Transketolase_fam
IPR005474 Transketolase_N
PANTHERiPTHR43522 PTHR43522, 1 hit
PfamiView protein in Pfam
PF02779 Transket_pyr, 1 hit
PF02780 Transketolase_C, 1 hit
PF00456 Transketolase_N, 1 hit
SMARTiView protein in SMART
SM00861 Transket_pyr, 1 hit
SUPFAMiSSF52518 SSF52518, 2 hits
SSF52922 SSF52922, 1 hit
TIGRFAMsiTIGR00232 tktlase_bact, 1 hit
PROSITEiView protein in PROSITE
PS00801 TRANSKETOLASE_1, 1 hit
PS00802 TRANSKETOLASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P33570-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRKDLANAI RALSMDAVQK ANSGHPGAPM GMADIAEVLW NDFLKHNPTD
60 70 80 90 100
PTWYDRDRFI LSNGHASMLL YSLLHLTGYD LPLEELKNFR QLHSKTPGHP
110 120 130 140 150
EIGYTPGVET TTGPLGQGLA NAVGLAIAER TLAAQFNQPD HEIVDHFTYV
160 170 180 190 200
FMGDGCLMEG ISHEVCSLAG TLGLGKLIGF YDHNGISIDG ETEGWFTDDT
210 220 230 240 250
AKRFEAYHWH VIHEIDGHDP QAVKEAILEA QSVKDKPSLI ICRTVIGFGS
260 270 280 290 300
PNKAGKEEAH GAPLGEEEVA LARQKLGWHH PPFEIPKEIY HAWDAREKGE
310 320 330 340 350
KAQQSWNEKF AAYKKAHPQL AEEFTRRMSG GLPKDWEKTT QKYINELQAN
360 370 380 390 400
PAKIATRKAS QNTLNAYGPM LPELLGGSAD LAPSNLTIWK GSVSLKEDPA
410 420 430 440 450
GNYIHYGVRE FGMTAIANGI AHHGGFVPYT ATFLMFVEYA RNAARMAALM
460 470 480 490 500
KARQIMVYTH DSIGLGEDGP THQAVEQLAS LRLTPNFSTW RPCDQVEAAV
510 520 530 540 550
GWKLAVERHN GPTALILSRQ NLAQVERTPD QVKEIARGGY VLKDSGGKPD
560 570 580 590 600
IILIATGSEM EITLQAAEKL AGEGRNVRVV SLPSTDIFDA QDEEYRESVL
610 620 630 640 650
PSNVAARVAV EAGIADYWYK YVGLKGAIVG MTGYGESAPA DKLFPFFGFT
660
AENIVAKAHK VLGVKGA
Length:667
Mass (Da):73,043
Last modified:February 1, 1994 - v1
Checksum:i451D748B13ED51E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12473 Genomic DNA Translation: BAA02039.1
U00096 Genomic DNA Translation: AAC75518.1
AP009048 Genomic DNA Translation: BAA16340.1
PIRiA48660
RefSeqiNP_416960.1, NC_000913.3
WP_000087280.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75518; AAC75518; b2465
BAA16340; BAA16340; BAA16340
GeneIDi945865
KEGGiecj:JW2449
eco:b2465
PATRICifig|511145.12.peg.2559

Similar proteinsi

Entry informationi

Entry nameiTKT2_ECOLI
AccessioniPrimary (citable) accession number: P33570
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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