Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Type 4 prepilin-like proteins leader peptide-processing enzyme

Gene

pilD

Organism
Neisseria gonorrhoeae
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue.By similarity

Catalytic activityi

Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Methyltransferase, Protease, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Type 4 prepilin-like proteins leader peptide-processing enzyme
Including the following 2 domains:
Leader peptidase (EC:3.4.23.43)
Alternative name(s):
Prepilin peptidase
N-methyltransferase (EC:2.1.1.-)
Gene namesi
Name:pilD
OrganismiNeisseria gonorrhoeae
Taxonomic identifieri485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3021HelicalSequence analysisAdd
BLAST
Transmembranei102 – 12221HelicalSequence analysisAdd
BLAST
Transmembranei126 – 14621HelicalSequence analysisAdd
BLAST
Transmembranei157 – 17721HelicalSequence analysisAdd
BLAST
Transmembranei181 – 20121HelicalSequence analysisAdd
BLAST
Transmembranei224 – 24421HelicalSequence analysisAdd
BLAST
Transmembranei250 – 27021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286Type 4 prepilin-like proteins leader peptide-processing enzymePRO_0000192624Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi528357.NgonPI_010100009518.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.

Sequencei

Sequence statusi: Complete.

P33566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLSVLSPF AVPLAAVLGL LVGSFLNVVI YRVPVMMERG WTVFAKEHLN
60 70 80 90 100
LPLTDDESRT FNLMKPDSCC PKCRVPIRAW QNIPIVSYLL LRGKCASCQT
110 120 130 140 150
KISIRYPLIE LLTGVLFGLV AWQYGWSWIT LGGLILTAFL ISLTFIDADT
160 170 180 190 200
QYLPDSMTLP LIWLGLIFNL DGGFVPLQSA VLGAVAGYSS LWLLCAVYKL
210 220 230 240 250
LTGKTGMGNG DFKLIAALGA WLGISALPVL IFVSSLIGLV AAIVMRVAKG
260 270 280
RHFAFGPALT VSGWIIFTAN DSVWRAVNWW LTHPVR
Length:286
Mass (Da):31,332
Last modified:November 1, 1997 - v2
Checksum:iA2941E7EDCACE275
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti148 – 1481A → E in AAB30050 (PubMed:7906688).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32588 Genomic DNA. Translation: AAC43468.1.
S69067 Genomic DNA. Translation: AAB30050.1.
PIRiA53374.
S32915.
RefSeqiWP_003689814.1. NZ_LOIB01000024.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32588 Genomic DNA. Translation: AAC43468.1.
S69067 Genomic DNA. Translation: AAB30050.1.
PIRiA53374.
S32915.
RefSeqiWP_003689814.1. NZ_LOIB01000024.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi528357.NgonPI_010100009518.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.
ProtoNetiSearch...

Entry informationi

Entry nameiLEP4_NEIGO
AccessioniPrimary (citable) accession number: P33566
Secondary accession number(s): Q50963, Q53404
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.