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P33563

- MTBB_BACIU

UniProt

P33563 - MTBB_BACIU

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Protein

Modification methylase BsuBI

Gene

hsdBM

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strand, and protects the DNA from cleavage by the BsuBI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3336. M.BsuBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BsuBI (EC:2.1.1.72)
Short name:
M.BsuBI
Alternative name(s):
Adenine-specific methyltransferase BsuBI
Gene namesi
Name:hsdBM
Synonyms:hsmB
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Modification methylase BsuBIPRO_0000087946Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP33563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF07669. Eco57I. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33563-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQILETVDK SRLTVNPLLK NKSELGQFFT PSSISIFMAC LFSEDKLNNA
60 70 80 90 100
KVLDAGAGIG SLTSAFLARL ISENIGKADL HLLEIDEMLE PYLSETLALF
110 120 130 140 150
KDYIEINSQI IIDDFIEWAA YSLLDEESLL AKDKQRFTHA ILNPPYKKIK
160 170 180 190 200
SNSKHRKLLR KAGIETVNLY SAFVALTVDL MSDGGEIVFI IPRSFCNGPY
210 220 230 240 250
FRHFRQHLLN KTSIKHMHLF ESRDKAFKDD EVLQENVISK LEKGTVQEDV
260 270 280 290 300
KISISTDDSF SVIRSYRYPF EKIVQPNDIE KFIHINTTNE ETLIEKHPNV
310 320 330 340 350
CYSLEELNIE VSTGPVVDFR VKENLREMPG EGTVPLFYPN HFVGTSLEYP
360 370 380 390 400
KMMKKPNAII RNEKVEKWLY PNGHYVVVKR FSSKEEKRRI VAGVLTPESV
410 420 430 440 450
NDPVVGFENG LNVLHYNKSG ISKEVAYGLY AYLNSTPVDK YFRIFNGHTQ
460 470 480 490 500
VNATDLRTMK FPSRDILISL GKWVIENIEN VGQVEIDSKL EELLLNDRGN

A
Length:501
Mass (Da):57,198
Last modified:February 1, 1994 - v1
Checksum:iAC33B2944699653E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01541 Genomic DNA. Translation: AAA18169.1.
PIRiS35515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01541 Genomic DNA. Translation: AAA18169.1 .
PIRi S35515.

3D structure databases

ProteinModelPortali P33563.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3336. M.BsuBI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF07669. Eco57I. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "BsuBI -- an isospecific restriction and modification system of PstI: characterization of the BsuBI genes and enzymes."
    Xu G.-L., Kapfer W., Walter J., Trautner T.A.
    Nucleic Acids Res. 20:6517-6523(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ISB8.

Entry informationi

Entry nameiMTBB_BACIU
AccessioniPrimary (citable) accession number: P33563
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 1, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3