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P33563

- MTBB_BACIU

UniProt

P33563 - MTBB_BACIU

Protein

Modification methylase BsuBI

Gene

hsdBM

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strand, and protects the DNA from cleavage by the BsuBI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3336. M.BsuBI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase BsuBI (EC:2.1.1.72)
    Short name:
    M.BsuBI
    Alternative name(s):
    Adenine-specific methyltransferase BsuBI
    Gene namesi
    Name:hsdBM
    Synonyms:hsmB
    OrganismiBacillus subtilis
    Taxonomic identifieri1423 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 501501Modification methylase BsuBIPRO_0000087946Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP33563.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR011639. RM_methylase_Eco57I.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF07669. Eco57I. 1 hit.
    [Graphical view]
    PRINTSiPR00507. N12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33563-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQILETVDK SRLTVNPLLK NKSELGQFFT PSSISIFMAC LFSEDKLNNA    50
    KVLDAGAGIG SLTSAFLARL ISENIGKADL HLLEIDEMLE PYLSETLALF 100
    KDYIEINSQI IIDDFIEWAA YSLLDEESLL AKDKQRFTHA ILNPPYKKIK 150
    SNSKHRKLLR KAGIETVNLY SAFVALTVDL MSDGGEIVFI IPRSFCNGPY 200
    FRHFRQHLLN KTSIKHMHLF ESRDKAFKDD EVLQENVISK LEKGTVQEDV 250
    KISISTDDSF SVIRSYRYPF EKIVQPNDIE KFIHINTTNE ETLIEKHPNV 300
    CYSLEELNIE VSTGPVVDFR VKENLREMPG EGTVPLFYPN HFVGTSLEYP 350
    KMMKKPNAII RNEKVEKWLY PNGHYVVVKR FSSKEEKRRI VAGVLTPESV 400
    NDPVVGFENG LNVLHYNKSG ISKEVAYGLY AYLNSTPVDK YFRIFNGHTQ 450
    VNATDLRTMK FPSRDILISL GKWVIENIEN VGQVEIDSKL EELLLNDRGN 500
    A 501
    Length:501
    Mass (Da):57,198
    Last modified:February 1, 1994 - v1
    Checksum:iAC33B2944699653E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01541 Genomic DNA. Translation: AAA18169.1.
    PIRiS35515.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01541 Genomic DNA. Translation: AAA18169.1 .
    PIRi S35515.

    3D structure databases

    ProteinModelPortali P33563.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3336. M.BsuBI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR011639. RM_methylase_Eco57I.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF07669. Eco57I. 1 hit.
    [Graphical view ]
    PRINTSi PR00507. N12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "BsuBI -- an isospecific restriction and modification system of PstI: characterization of the BsuBI genes and enzymes."
      Xu G.-L., Kapfer W., Walter J., Trautner T.A.
      Nucleic Acids Res. 20:6517-6523(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ISB8.

    Entry informationi

    Entry nameiMTBB_BACIU
    AccessioniPrimary (citable) accession number: P33563
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3