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Protein

Modification methylase BsuBI

Gene

hsdBM

Organism
Bacillus subtilis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strand, and protects the DNA from cleavage by the BsuBI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3336. M.BsuBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BsuBI (EC:2.1.1.72)
Short name:
M.BsuBI
Alternative name(s):
Adenine-specific methyltransferase BsuBI
Gene namesi
Name:hsdBM
Synonyms:hsmB
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Modification methylase BsuBIPRO_0000087946Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP33563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF07669. Eco57I. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQILETVDK SRLTVNPLLK NKSELGQFFT PSSISIFMAC LFSEDKLNNA
60 70 80 90 100
KVLDAGAGIG SLTSAFLARL ISENIGKADL HLLEIDEMLE PYLSETLALF
110 120 130 140 150
KDYIEINSQI IIDDFIEWAA YSLLDEESLL AKDKQRFTHA ILNPPYKKIK
160 170 180 190 200
SNSKHRKLLR KAGIETVNLY SAFVALTVDL MSDGGEIVFI IPRSFCNGPY
210 220 230 240 250
FRHFRQHLLN KTSIKHMHLF ESRDKAFKDD EVLQENVISK LEKGTVQEDV
260 270 280 290 300
KISISTDDSF SVIRSYRYPF EKIVQPNDIE KFIHINTTNE ETLIEKHPNV
310 320 330 340 350
CYSLEELNIE VSTGPVVDFR VKENLREMPG EGTVPLFYPN HFVGTSLEYP
360 370 380 390 400
KMMKKPNAII RNEKVEKWLY PNGHYVVVKR FSSKEEKRRI VAGVLTPESV
410 420 430 440 450
NDPVVGFENG LNVLHYNKSG ISKEVAYGLY AYLNSTPVDK YFRIFNGHTQ
460 470 480 490 500
VNATDLRTMK FPSRDILISL GKWVIENIEN VGQVEIDSKL EELLLNDRGN

A
Length:501
Mass (Da):57,198
Last modified:February 1, 1994 - v1
Checksum:iAC33B2944699653E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01541 Genomic DNA. Translation: AAA18169.1.
PIRiS35515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01541 Genomic DNA. Translation: AAA18169.1.
PIRiS35515.

3D structure databases

ProteinModelPortaliP33563.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3336. M.BsuBI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF07669. Eco57I. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "BsuBI -- an isospecific restriction and modification system of PstI: characterization of the BsuBI genes and enzymes."
    Xu G.-L., Kapfer W., Walter J., Trautner T.A.
    Nucleic Acids Res. 20:6517-6523(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ISB8.

Entry informationi

Entry nameiMTBB_BACIU
AccessioniPrimary (citable) accession number: P33563
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 29, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.