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P33563 (MTBB_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase BsuBI

Short name=M.BsuBI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase BsuBI
Gene names
Name:hsdBM
Synonyms:hsmB
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strand, and protects the DNA from cleavage by the BsuBI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Modification methylase BsuBI
PRO_0000087946

Sequences

Sequence LengthMass (Da)Tools
P33563 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: AC33B2944699653E

FASTA50157,198
        10         20         30         40         50         60 
MTQILETVDK SRLTVNPLLK NKSELGQFFT PSSISIFMAC LFSEDKLNNA KVLDAGAGIG 

        70         80         90        100        110        120 
SLTSAFLARL ISENIGKADL HLLEIDEMLE PYLSETLALF KDYIEINSQI IIDDFIEWAA 

       130        140        150        160        170        180 
YSLLDEESLL AKDKQRFTHA ILNPPYKKIK SNSKHRKLLR KAGIETVNLY SAFVALTVDL 

       190        200        210        220        230        240 
MSDGGEIVFI IPRSFCNGPY FRHFRQHLLN KTSIKHMHLF ESRDKAFKDD EVLQENVISK 

       250        260        270        280        290        300 
LEKGTVQEDV KISISTDDSF SVIRSYRYPF EKIVQPNDIE KFIHINTTNE ETLIEKHPNV 

       310        320        330        340        350        360 
CYSLEELNIE VSTGPVVDFR VKENLREMPG EGTVPLFYPN HFVGTSLEYP KMMKKPNAII 

       370        380        390        400        410        420 
RNEKVEKWLY PNGHYVVVKR FSSKEEKRRI VAGVLTPESV NDPVVGFENG LNVLHYNKSG 

       430        440        450        460        470        480 
ISKEVAYGLY AYLNSTPVDK YFRIFNGHTQ VNATDLRTMK FPSRDILISL GKWVIENIEN 

       490        500 
VGQVEIDSKL EELLLNDRGN A 

« Hide

References

[1]"BsuBI -- an isospecific restriction and modification system of PstI: characterization of the BsuBI genes and enzymes."
Xu G.-L., Kapfer W., Walter J., Trautner T.A.
Nucleic Acids Res. 20:6517-6523(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ISB8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L01541 Genomic DNA. Translation: AAA18169.1.
PIRS35515.

3D structure databases

ProteinModelPortalP33563.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3336. M.BsuBI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
[Graphical view]
PfamPF07669. Eco57I. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTBB_BACIU
AccessionPrimary (citable) accession number: P33563
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries