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P33562

- T2BB_BACIU

UniProt

P33562 - T2BB_BACIU

Protein

Type-2 restriction enzyme BsuBI

Gene

hsdBR

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CTGCAG and cleaves after A-5.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. magnesium ion binding Source: InterPro
    3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium

    Protein family/group databases

    REBASEi616. BsuBI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme BsuBI (EC:3.1.21.4)
    Short name:
    R.BsuBI
    Alternative name(s):
    Endonuclease BsuBI
    Type II restriction enzyme BsuBI
    Gene namesi
    Name:hsdBR
    Synonyms:hsrB
    OrganismiBacillus subtilis
    Taxonomic identifieri1423 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 316316Type-2 restriction enzyme BsuBIPRO_0000077290Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP33562.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR009528. Restrct_endonuc_II_BsuBI_C.
    [Graphical view]
    PfamiPF06616. BsuBI_PstI_RE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33562-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEGMHSNVK EAIKILKELG LPKGQQNERS ALCLLSLMNI TQDKTWSEAE    50
    SPLIGITPMM EFCRINYGKE YAPNSRETFR RFTMHQFVDA GIALYNPDKP 100
    TRPVNSPKAV YQIEAETLEL IKCYNTEEWS ELLARYLSNR QTLVERYAKE 150
    RQQNKIPVQI AEGKEIYITP GEHSELIKAI IEEFAPRYVP GGRLIYAGDT 200
    GEKMGYFDEE LLRQLGVVID SHGKMPDVVI YFPEKKWLLL IESVTSHGPV 250
    DHKRHEELAK LFNGSTAGIV YVTAFPNRSL MARYLNNISW ETEVWVADAP 300
    SHLIHFNGVR FLGPYE 316
    Length:316
    Mass (Da):36,197
    Last modified:February 1, 1994 - v1
    Checksum:iB1C2E45851E7F3C9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01541 Genomic DNA. Translation: AAA18170.1.
    PIRiS35516.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01541 Genomic DNA. Translation: AAA18170.1 .
    PIRi S35516.

    3D structure databases

    ProteinModelPortali P33562.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 616. BsuBI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR009528. Restrct_endonuc_II_BsuBI_C.
    [Graphical view ]
    Pfami PF06616. BsuBI_PstI_RE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "BsuBI -- an isospecific restriction and modification system of PstI: characterization of the BsuBI genes and enzymes."
      Xu G.-L., Kapfer W., Walter J., Trautner T.A.
      Nucleic Acids Res. 20:6517-6523(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ISB8.

    Entry informationi

    Entry nameiT2BB_BACIU
    AccessioniPrimary (citable) accession number: P33562
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3