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P33562 (T2BB_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme BsuBI

Short name=R.BsuBI
EC=3.1.21.4
Alternative name(s):
Endonuclease BsuBI
Type II restriction enzyme BsuBI
Gene names
Name:hsdBR
Synonyms:hsrB
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Recognizes the double-stranded sequence CTGCAG and cleaves after A-5.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the BsuBI/PstI restriction endonuclease family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316Type-2 restriction enzyme BsuBI
PRO_0000077290

Sequences

Sequence LengthMass (Da)Tools
P33562 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: B1C2E45851E7F3C9

FASTA31636,197
        10         20         30         40         50         60 
MTEGMHSNVK EAIKILKELG LPKGQQNERS ALCLLSLMNI TQDKTWSEAE SPLIGITPMM 

        70         80         90        100        110        120 
EFCRINYGKE YAPNSRETFR RFTMHQFVDA GIALYNPDKP TRPVNSPKAV YQIEAETLEL 

       130        140        150        160        170        180 
IKCYNTEEWS ELLARYLSNR QTLVERYAKE RQQNKIPVQI AEGKEIYITP GEHSELIKAI 

       190        200        210        220        230        240 
IEEFAPRYVP GGRLIYAGDT GEKMGYFDEE LLRQLGVVID SHGKMPDVVI YFPEKKWLLL 

       250        260        270        280        290        300 
IESVTSHGPV DHKRHEELAK LFNGSTAGIV YVTAFPNRSL MARYLNNISW ETEVWVADAP 

       310 
SHLIHFNGVR FLGPYE 

« Hide

References

[1]"BsuBI -- an isospecific restriction and modification system of PstI: characterization of the BsuBI genes and enzymes."
Xu G.-L., Kapfer W., Walter J., Trautner T.A.
Nucleic Acids Res. 20:6517-6523(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ISB8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L01541 Genomic DNA. Translation: AAA18170.1.
PIRS35516.

3D structure databases

ProteinModelPortalP33562.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE616. BsuBI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR009528. Restrct_endonuc_II_BsuBI_C.
[Graphical view]
PfamPF06616. BsuBI_PstI_RE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2BB_BACIU
AccessionPrimary (citable) accession number: P33562
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries