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Protein

Type-2 restriction enzyme BsuBI

Gene

hsdBR

Organism
Bacillus subtilis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Recognizes the double-stranded sequence CTGCAG and cleaves after A-5.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium

Protein family/group databases

REBASEi616. BsuBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BsuBI (EC:3.1.21.4)
Short name:
R.BsuBI
Alternative name(s):
Endonuclease BsuBI
Type II restriction enzyme BsuBI
Gene namesi
Name:hsdBR
Synonyms:hsrB
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Type-2 restriction enzyme BsuBIPRO_0000077290Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP33562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR009528. Restrct_endonuc_II_BsuBI_C.
[Graphical view]
PfamiPF06616. BsuBI_PstI_RE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEGMHSNVK EAIKILKELG LPKGQQNERS ALCLLSLMNI TQDKTWSEAE
60 70 80 90 100
SPLIGITPMM EFCRINYGKE YAPNSRETFR RFTMHQFVDA GIALYNPDKP
110 120 130 140 150
TRPVNSPKAV YQIEAETLEL IKCYNTEEWS ELLARYLSNR QTLVERYAKE
160 170 180 190 200
RQQNKIPVQI AEGKEIYITP GEHSELIKAI IEEFAPRYVP GGRLIYAGDT
210 220 230 240 250
GEKMGYFDEE LLRQLGVVID SHGKMPDVVI YFPEKKWLLL IESVTSHGPV
260 270 280 290 300
DHKRHEELAK LFNGSTAGIV YVTAFPNRSL MARYLNNISW ETEVWVADAP
310
SHLIHFNGVR FLGPYE
Length:316
Mass (Da):36,197
Last modified:February 1, 1994 - v1
Checksum:iB1C2E45851E7F3C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01541 Genomic DNA. Translation: AAA18170.1.
PIRiS35516.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01541 Genomic DNA. Translation: AAA18170.1.
PIRiS35516.

3D structure databases

ProteinModelPortaliP33562.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi616. BsuBI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR009528. Restrct_endonuc_II_BsuBI_C.
[Graphical view]
PfamiPF06616. BsuBI_PstI_RE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "BsuBI -- an isospecific restriction and modification system of PstI: characterization of the BsuBI genes and enzymes."
    Xu G.-L., Kapfer W., Walter J., Trautner T.A.
    Nucleic Acids Res. 20:6517-6523(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ISB8.

Entry informationi

Entry nameiT2BB_BACIU
AccessioniPrimary (citable) accession number: P33562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 27, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.