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P33562

- T2BB_BACIU

UniProt

P33562 - T2BB_BACIU

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Protein
Type-2 restriction enzyme BsuBI
Gene
hsdBR, hsrB
Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Recognizes the double-stranded sequence CTGCAG and cleaves after A-5.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Magnesium.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC
  3. magnesium ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium

Protein family/group databases

REBASEi616. BsuBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BsuBI (EC:3.1.21.4)
Short name:
R.BsuBI
Alternative name(s):
Endonuclease BsuBI
Type II restriction enzyme BsuBI
Gene namesi
Name:hsdBR
Synonyms:hsrB
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Type-2 restriction enzyme BsuBI
PRO_0000077290Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP33562.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR009528. Restrct_endonuc_II_BsuBI_C.
[Graphical view]
PfamiPF06616. BsuBI_PstI_RE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33562-1 [UniParc]FASTAAdd to Basket

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MTEGMHSNVK EAIKILKELG LPKGQQNERS ALCLLSLMNI TQDKTWSEAE    50
SPLIGITPMM EFCRINYGKE YAPNSRETFR RFTMHQFVDA GIALYNPDKP 100
TRPVNSPKAV YQIEAETLEL IKCYNTEEWS ELLARYLSNR QTLVERYAKE 150
RQQNKIPVQI AEGKEIYITP GEHSELIKAI IEEFAPRYVP GGRLIYAGDT 200
GEKMGYFDEE LLRQLGVVID SHGKMPDVVI YFPEKKWLLL IESVTSHGPV 250
DHKRHEELAK LFNGSTAGIV YVTAFPNRSL MARYLNNISW ETEVWVADAP 300
SHLIHFNGVR FLGPYE 316
Length:316
Mass (Da):36,197
Last modified:February 1, 1994 - v1
Checksum:iB1C2E45851E7F3C9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01541 Genomic DNA. Translation: AAA18170.1.
PIRiS35516.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01541 Genomic DNA. Translation: AAA18170.1 .
PIRi S35516.

3D structure databases

ProteinModelPortali P33562.
ModBasei Search...

Protein family/group databases

REBASEi 616. BsuBI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR009528. Restrct_endonuc_II_BsuBI_C.
[Graphical view ]
Pfami PF06616. BsuBI_PstI_RE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "BsuBI -- an isospecific restriction and modification system of PstI: characterization of the BsuBI genes and enzymes."
    Xu G.-L., Kapfer W., Walter J., Trautner T.A.
    Nucleic Acids Res. 20:6517-6523(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ISB8.

Entry informationi

Entry nameiT2BB_BACIU
AccessioniPrimary (citable) accession number: P33562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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