P33559 (XYNA_ASPKW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endo-1,4-beta-xylanase A
Short name=Xylanase A
EC=3.2.1.8

Alternative name(s):
1,4-beta-D-xylan xylanohydrolase A

Gene names

Name:	xynA
ORF Names:	AKAW_10666

Organism

Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi) [Complete proteome]

Taxonomic identifier

1033177 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus kawachii

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
Pathway	Glycan degradation; xylan degradation.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 10 (cellulase F) family.
Sequence caution	The sequence GAA92552.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Xylan degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	endo-1,4-beta-xylanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.1
	Chain	26 – 327	302	Endo-1,4-beta-xylanase A		PRO_0000007965
Sites
	Active site	157	1	Proton donor By similarity
	Active site	263	1	Nucleophile By similarity
Amino acid modifications
	Modified residue	26	1	Pyrrolidone carboxylic acid
	Disulfide bond	281 ↔ 287		By similarity
Experimental info
	Sequence conflict	33	1	T → S in BAA03575. Ref.1
	Sequence conflict	33	1	T → S AA sequence Ref.1
	Sequence conflict	61	1	I → V in BAA03575. Ref.1
	Sequence conflict	176	1	E → D in BAA03575. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P33559 [UniParc]. Last modified March 21, 2012. Version 2. Checksum: 93B3D04F22F5D4FF	FASTA	327	35,480
	10 20 30 40 50 60 MVQIKAAALA MLFASHVLSE PIEPRQASVS IDTKFKAHGK KYLGNIGDQY TLTKNSKTPA 70 80 90 100 110 120 IIKADFGALT PENSMKWDAT EPSRGQFSFS GSDYLVNFAQ SNNKLIRGHT LVWHSQLPSW 130 140 150 160 170 180 VQAITDKNTL IEVMKNHITT VMQHYKGKIY AWDVVNEIFN EDGSLRDSVF YKVIGEDYVR 190 200 210 220 230 240 IAFETARAAD PNAKLYINDY NLDSASYPKL AGMVSHVKKW IEAGIPIDGI GSQTHLSAGG 250 260 270 280 290 300 GAGISGALNA LAGAGTKEIA VTELDIAGAS STDYVEVVEA CLDQPKCIGI TVWGVADPDS 310 320 WRSSSTPLLF DSNYNPKPAY TAIANAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the xynA gene encoding xylanase A of Aspergillus kawachii." Ito K., Ikemasu T., Ishikawa T. Biosci. Biotechnol. Biochem. 56:906-912(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-33; 41-54; 63-76 AND 76-100. Strain: NBRC 4308.
[2]	"Genome sequence of the white koji mold Aspergillus kawachii IFO 4308, used for brewing the Japanese distilled spirit shochu." Futagami T., Mori K., Yamashita A., Wada S., Kajiwara Y., Takashita H., Omori T., Takegawa K., Tashiro K., Kuhara S., Goto M. Eukaryot. Cell 10:1586-1587(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NBRC 4308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14847 Genomic DNA. Translation: BAA03575.1. DF126496 Genomic DNA. Translation: GAA92552.1. Sequence problems.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH10. Glycoside Hydrolase Family 10.
mycoCLAP	XYN10A_ASPKA.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00114.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001000. Glyco_hydro_10. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00331. Glyco_hydro_10. 1 hit. [Graphical view]
PRINTS	PR00134. GLHYDRLASE10.
SMART	SM00633. Glyco_10. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00591. GLYCOSYL_HYDROL_F10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYNA_ASPKW

Accession

Primary (citable) accession number: P33559
Secondary accession number(s): G7Y054

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	March 21, 2012
Last modified:	May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program