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P33558

- XYNA2_CLOSR

UniProt

P33558 - XYNA2_CLOSR

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Protein

Endo-1,4-beta-xylanase A

Gene

xynA

Organism
Clostridium stercorarium
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

Temperature dependencei

Optimum temperature is 70 degrees Celsius. Thermostable.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei124 – 1241NucleophilePROSITE-ProRule annotation
Active sitei215 – 2151Proton donorPROSITE-ProRule annotation
Metal bindingi254 – 2541Calcium 1By similarity
Metal bindingi256 – 2561Calcium 1By similarity
Binding sitei271 – 2711Substrate 1; via carbonyl oxygenBy similarity
Metal bindingi276 – 2761Calcium 1; via carbonyl oxygenBy similarity
Binding sitei280 – 2801Substrate 1; via amide nitrogenBy similarity
Binding sitei337 – 3371Substrate 1By similarity
Binding sitei364 – 3641Substrate 1By similarity
Metal bindingi366 – 3661Calcium 1By similarity
Metal bindingi391 – 3911Calcium 2By similarity
Metal bindingi393 – 3931Calcium 2By similarity
Metal bindingi413 – 4131Calcium 2; via carbonyl oxygenBy similarity
Binding sitei417 – 4171Substrate 2; via amide nitrogenBy similarity
Binding sitei474 – 4741Substrate 2By similarity
Binding sitei501 – 5011Substrate 2By similarity
Metal bindingi503 – 5031Calcium 2By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. endo-1,4-beta-xylanase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. xylan catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation, Xylan degradation

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00114.

Protein family/group databases

CAZyiCBM6. Carbohydrate-Binding Module Family 6.
GH11. Glycoside Hydrolase Family 11.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,4-beta-xylanase A (EC:3.2.1.8)
Short name:
Xylanase A
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase A
Gene namesi
Name:xynA
OrganismiClostridium stercorarium
Taxonomic identifieri1510 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 512482Endo-1,4-beta-xylanase APRO_0000008002Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP33558.
SMRiP33558. Positions 33-374, 379-509.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini251 – 371121CBM6 1PROSITE-ProRule annotationAdd
BLAST
Repeati279 – 340621Add
BLAST
Domaini388 – 508121CBM6 2PROSITE-ProRule annotationAdd
BLAST
Repeati416 – 477622Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni279 – 4771992 X 61 AA approximate repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi236 – 2449Pro-rich

Domaini

XynA is a modular enzyme. The number of CBM6 (carbohydrate binding type-6) domains varies between strains. The polymeric substrate can interact with several of these CBM6 domains (By similarity).By similarity

Sequence similaritiesi

Contains 2 CBM6 (carbohydrate binding type-6) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.120.180. 1 hit.
2.60.120.260. 2 hits.
InterProiIPR006584. Cellulose-bd_IV.
IPR005084. CMB_fam6.
IPR013320. ConA-like_dom.
IPR008979. Galactose-bd-like.
IPR001137. Glyco_hydro_11.
IPR013319. Glyco_hydro_11/12.
IPR018208. Glyco_hydro_11_AS.
[Graphical view]
PfamiPF03422. CBM_6. 2 hits.
PF00457. Glyco_hydro_11. 1 hit.
[Graphical view]
PRINTSiPR00911. GLHYDRLASE11.
SMARTiSM00606. CBD_IV. 2 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiPS51175. CBM6. 2 hits.
PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33558-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRKVKKMAA MATSIIMAIM IILHSIPVLA GRIIYDNETG THGGYDYELW
60 70 80 90 100
KDYGNTIMEL NDGGTFSCQW SNIGNALFRK GRKFNSDKTY QELGDIVVEY
110 120 130 140 150
GCDYNPNGNS YLCVYGWTRN PLVEYYIVES WGSWRPPGAT PKGTITQWMA
160 170 180 190 200
GTYEIYETTR VNQPSIDGTA TFQQYWSVRT SKRTSGTISV TEHFKQWERM
210 220 230 240 250
GMRMGKMYEV ALTVEGYQSS GYANVYKNEI RIGANPTPAP SQSPIRRDAF
260 270 280 290 300
SIIEAEEYNS TNSSTLQVIG TPNNGRGIGY IENGNTVTYS NIDFGSGATG
310 320 330 340 350
FSATVATEVN TSIQIRSDSP TGTLLGTLYV SSTGSWNTYQ TVSTNISKIT
360 370 380 390 400
GVHDIVLVFS GPVNVDNFIF SRSSPVPAPG DNTRDAYSII QAEDYDSSYG
410 420 430 440 450
PNLQIFSLPG GGSAIGYIEN GYSTTYKNID FGDGATSVTA RVATQNATTI
460 470 480 490 500
QVRLGSPSGT LLGTIYVGST GSFDTYRDVS ATISNTAGVK DIVLVFSGPV
510
NVDWFVFSKS GT
Length:512
Mass (Da):55,843
Last modified:February 1, 1996 - v2
Checksum:i1E133CBF4C139305
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13325 Genomic DNA. Translation: BAA02584.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13325 Genomic DNA. Translation: BAA02584.1 .

3D structure databases

ProteinModelPortali P33558.
SMRi P33558. Positions 33-374, 379-509.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM6. Carbohydrate-Binding Module Family 6.
GH11. Glycoside Hydrolase Family 11.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00114 .

Family and domain databases

Gene3Di 2.60.120.180. 1 hit.
2.60.120.260. 2 hits.
InterProi IPR006584. Cellulose-bd_IV.
IPR005084. CMB_fam6.
IPR013320. ConA-like_dom.
IPR008979. Galactose-bd-like.
IPR001137. Glyco_hydro_11.
IPR013319. Glyco_hydro_11/12.
IPR018208. Glyco_hydro_11_AS.
[Graphical view ]
Pfami PF03422. CBM_6. 2 hits.
PF00457. Glyco_hydro_11. 1 hit.
[Graphical view ]
PRINTSi PR00911. GLHYDRLASE11.
SMARTi SM00606. CBD_IV. 2 hits.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEi PS51175. CBM6. 2 hits.
PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the Clostridium stercorarium xynA gene encoding xylanase A: identification of catalytic and cellulose binding domains."
    Sakka K., Kojima Y., Kondo T., Karita S., Ohmiya K., Shimada K.
    Biosci. Biotechnol. Biochem. 57:273-277(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-40.
    Strain: F-9.
  2. Sakka K.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiXYNA2_CLOSR
AccessioniPrimary (citable) accession number: P33558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3