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Protein

Endo-1,4-beta-xylanase A

Gene

xynA

Organism
Clostridium stercorarium
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

Temperature dependencei

Optimum temperature is 70 degrees Celsius. Thermostable.

Pathwayi: xylan degradation

This protein is involved in the pathway xylan degradation, which is part of Glycan degradation.
View all proteins of this organism that are known to be involved in the pathway xylan degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei124NucleophilePROSITE-ProRule annotation1
Active sitei215Proton donorPROSITE-ProRule annotation1
Metal bindingi254Calcium 1By similarity1
Metal bindingi256Calcium 1By similarity1
Binding sitei271Substrate 1; via carbonyl oxygenBy similarity1
Metal bindingi276Calcium 1; via carbonyl oxygenBy similarity1
Binding sitei280Substrate 1; via amide nitrogenBy similarity1
Binding sitei337Substrate 1By similarity1
Binding sitei364Substrate 1By similarity1
Metal bindingi366Calcium 1By similarity1
Metal bindingi391Calcium 2By similarity1
Metal bindingi393Calcium 2By similarity1
Metal bindingi413Calcium 2; via carbonyl oxygenBy similarity1
Binding sitei417Substrate 2; via amide nitrogenBy similarity1
Binding sitei474Substrate 2By similarity1
Binding sitei501Substrate 2By similarity1
Metal bindingi503Calcium 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation, Xylan degradation

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00114.

Protein family/group databases

CAZyiCBM6. Carbohydrate-Binding Module Family 6.
GH11. Glycoside Hydrolase Family 11.
mycoCLAPiXYN11A_CLOST.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,4-beta-xylanase A (EC:3.2.1.8)
Short name:
Xylanase A
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase A
Gene namesi
Name:xynA
OrganismiClostridium stercorarium
Taxonomic identifieri1510 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000000800231 – 512Endo-1,4-beta-xylanase AAdd BLAST482

Structurei

3D structure databases

ProteinModelPortaliP33558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 228GH11PROSITE-ProRule annotationAdd BLAST196
Domaini251 – 371CBM6 1PROSITE-ProRule annotationAdd BLAST121
Repeati279 – 3401Add BLAST62
Domaini388 – 508CBM6 2PROSITE-ProRule annotationAdd BLAST121
Repeati416 – 4772Add BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni279 – 4772 X 61 AA approximate repeatsAdd BLAST199

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi236 – 244Pro-rich9

Domaini

XynA is a modular enzyme. The number of CBM6 (carbohydrate binding type-6) domains varies between strains. The polymeric substrate can interact with several of these CBM6 domains (By similarity).By similarity

Sequence similaritiesi

Contains 2 CBM6 (carbohydrate binding type-6) domains.PROSITE-ProRule annotation
Contains 1 GH11 (glycosyl hydrolase family 11) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.120.180. 1 hit.
2.60.120.260. 2 hits.
InterProiIPR006584. Cellulose-bd_IV.
IPR005084. CMB_fam6.
IPR013320. ConA-like_dom.
IPR008979. Galactose-bd-like.
IPR013319. GH11/12.
IPR018208. GH11_AS_1.
IPR033119. GH11_AS_2.
IPR033123. GH11_dom.
IPR001137. Glyco_hydro_11.
[Graphical view]
PfamiPF03422. CBM_6. 2 hits.
PF00457. Glyco_hydro_11. 1 hit.
[Graphical view]
PRINTSiPR00911. GLHYDRLASE11.
SMARTiSM00606. CBD_IV. 2 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiPS51175. CBM6. 2 hits.
PS00776. GH11_1. 1 hit.
PS00777. GH11_2. 1 hit.
PS51761. GH11_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRKVKKMAA MATSIIMAIM IILHSIPVLA GRIIYDNETG THGGYDYELW
60 70 80 90 100
KDYGNTIMEL NDGGTFSCQW SNIGNALFRK GRKFNSDKTY QELGDIVVEY
110 120 130 140 150
GCDYNPNGNS YLCVYGWTRN PLVEYYIVES WGSWRPPGAT PKGTITQWMA
160 170 180 190 200
GTYEIYETTR VNQPSIDGTA TFQQYWSVRT SKRTSGTISV TEHFKQWERM
210 220 230 240 250
GMRMGKMYEV ALTVEGYQSS GYANVYKNEI RIGANPTPAP SQSPIRRDAF
260 270 280 290 300
SIIEAEEYNS TNSSTLQVIG TPNNGRGIGY IENGNTVTYS NIDFGSGATG
310 320 330 340 350
FSATVATEVN TSIQIRSDSP TGTLLGTLYV SSTGSWNTYQ TVSTNISKIT
360 370 380 390 400
GVHDIVLVFS GPVNVDNFIF SRSSPVPAPG DNTRDAYSII QAEDYDSSYG
410 420 430 440 450
PNLQIFSLPG GGSAIGYIEN GYSTTYKNID FGDGATSVTA RVATQNATTI
460 470 480 490 500
QVRLGSPSGT LLGTIYVGST GSFDTYRDVS ATISNTAGVK DIVLVFSGPV
510
NVDWFVFSKS GT
Length:512
Mass (Da):55,843
Last modified:February 1, 1996 - v2
Checksum:i1E133CBF4C139305
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13325 Genomic DNA. Translation: BAA02584.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13325 Genomic DNA. Translation: BAA02584.1.

3D structure databases

ProteinModelPortaliP33558.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM6. Carbohydrate-Binding Module Family 6.
GH11. Glycoside Hydrolase Family 11.
mycoCLAPiXYN11A_CLOST.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00114.

Family and domain databases

Gene3Di2.60.120.180. 1 hit.
2.60.120.260. 2 hits.
InterProiIPR006584. Cellulose-bd_IV.
IPR005084. CMB_fam6.
IPR013320. ConA-like_dom.
IPR008979. Galactose-bd-like.
IPR013319. GH11/12.
IPR018208. GH11_AS_1.
IPR033119. GH11_AS_2.
IPR033123. GH11_dom.
IPR001137. Glyco_hydro_11.
[Graphical view]
PfamiPF03422. CBM_6. 2 hits.
PF00457. Glyco_hydro_11. 1 hit.
[Graphical view]
PRINTSiPR00911. GLHYDRLASE11.
SMARTiSM00606. CBD_IV. 2 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiPS51175. CBM6. 2 hits.
PS00776. GH11_1. 1 hit.
PS00777. GH11_2. 1 hit.
PS51761. GH11_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXYNA2_CLOSR
AccessioniPrimary (citable) accession number: P33558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1996
Last modified: October 5, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.