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P33558

- XYNA2_CLOSR

UniProt

P33558 - XYNA2_CLOSR

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Protein
Endo-1,4-beta-xylanase A
Gene
xynA
Organism
Clostridium stercorarium
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

Temperature dependencei

Optimum temperature is 70 degrees Celsius. Thermostable.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei124 – 1241Nucleophile By similarity
Active sitei215 – 2151Proton donor By similarity
Metal bindingi254 – 2541Calcium 1 By similarity
Metal bindingi256 – 2561Calcium 1 By similarity
Binding sitei271 – 2711Substrate 1; via carbonyl oxygen By similarity
Metal bindingi276 – 2761Calcium 1; via carbonyl oxygen By similarity
Binding sitei280 – 2801Substrate 1; via amide nitrogen By similarity
Binding sitei337 – 3371Substrate 1 By similarity
Binding sitei364 – 3641Substrate 1 By similarity
Metal bindingi366 – 3661Calcium 1 By similarity
Metal bindingi391 – 3911Calcium 2 By similarity
Metal bindingi393 – 3931Calcium 2 By similarity
Metal bindingi413 – 4131Calcium 2; via carbonyl oxygen By similarity
Binding sitei417 – 4171Substrate 2; via amide nitrogen By similarity
Binding sitei474 – 4741Substrate 2 By similarity
Binding sitei501 – 5011Substrate 2 By similarity
Metal bindingi503 – 5031Calcium 2 By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. endo-1,4-beta-xylanase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. xylan catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation, Xylan degradation

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00114.

Protein family/group databases

CAZyiCBM6. Carbohydrate-Binding Module Family 6.
GH11. Glycoside Hydrolase Family 11.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,4-beta-xylanase A (EC:3.2.1.8)
Short name:
Xylanase A
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase A
Gene namesi
Name:xynA
OrganismiClostridium stercorarium
Taxonomic identifieri1510 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030 Reviewed prediction
Add
BLAST
Chaini31 – 512482Endo-1,4-beta-xylanase A
PRO_0000008002Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP33558.
SMRiP33558. Positions 33-374, 379-509.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini251 – 371121CBM6 1
Add
BLAST
Repeati279 – 340621
Add
BLAST
Domaini388 – 508121CBM6 2
Add
BLAST
Repeati416 – 477622
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni279 – 4771992 X 61 AA approximate repeats
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi236 – 2449Pro-rich

Domaini

XynA is a modular enzyme. The number of CBM6 (carbohydrate binding type-6) domains varies between strains. The polymeric substrate can interact with several of these CBM6 domains By similarity.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.120.180. 1 hit.
2.60.120.260. 2 hits.
InterProiIPR006584. Cellulose-bd_IV.
IPR005084. CMB_fam6.
IPR008985. ConA-like_lec_gl_sf.
IPR008979. Galactose-bd-like.
IPR001137. Glyco_hydro_11.
IPR013319. Glyco_hydro_11/12.
IPR018208. Glyco_hydro_11_AS.
[Graphical view]
PfamiPF03422. CBM_6. 2 hits.
PF00457. Glyco_hydro_11. 1 hit.
[Graphical view]
PRINTSiPR00911. GLHYDRLASE11.
SMARTiSM00606. CBD_IV. 2 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiPS51175. CBM6. 2 hits.
PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33558-1 [UniParc]FASTAAdd to Basket

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MKRKVKKMAA MATSIIMAIM IILHSIPVLA GRIIYDNETG THGGYDYELW    50
KDYGNTIMEL NDGGTFSCQW SNIGNALFRK GRKFNSDKTY QELGDIVVEY 100
GCDYNPNGNS YLCVYGWTRN PLVEYYIVES WGSWRPPGAT PKGTITQWMA 150
GTYEIYETTR VNQPSIDGTA TFQQYWSVRT SKRTSGTISV TEHFKQWERM 200
GMRMGKMYEV ALTVEGYQSS GYANVYKNEI RIGANPTPAP SQSPIRRDAF 250
SIIEAEEYNS TNSSTLQVIG TPNNGRGIGY IENGNTVTYS NIDFGSGATG 300
FSATVATEVN TSIQIRSDSP TGTLLGTLYV SSTGSWNTYQ TVSTNISKIT 350
GVHDIVLVFS GPVNVDNFIF SRSSPVPAPG DNTRDAYSII QAEDYDSSYG 400
PNLQIFSLPG GGSAIGYIEN GYSTTYKNID FGDGATSVTA RVATQNATTI 450
QVRLGSPSGT LLGTIYVGST GSFDTYRDVS ATISNTAGVK DIVLVFSGPV 500
NVDWFVFSKS GT 512
Length:512
Mass (Da):55,843
Last modified:February 1, 1996 - v2
Checksum:i1E133CBF4C139305
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13325 Genomic DNA. Translation: BAA02584.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13325 Genomic DNA. Translation: BAA02584.1 .

3D structure databases

ProteinModelPortali P33558.
SMRi P33558. Positions 33-374, 379-509.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM6. Carbohydrate-Binding Module Family 6.
GH11. Glycoside Hydrolase Family 11.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00114 .

Family and domain databases

Gene3Di 2.60.120.180. 1 hit.
2.60.120.260. 2 hits.
InterProi IPR006584. Cellulose-bd_IV.
IPR005084. CMB_fam6.
IPR008985. ConA-like_lec_gl_sf.
IPR008979. Galactose-bd-like.
IPR001137. Glyco_hydro_11.
IPR013319. Glyco_hydro_11/12.
IPR018208. Glyco_hydro_11_AS.
[Graphical view ]
Pfami PF03422. CBM_6. 2 hits.
PF00457. Glyco_hydro_11. 1 hit.
[Graphical view ]
PRINTSi PR00911. GLHYDRLASE11.
SMARTi SM00606. CBD_IV. 2 hits.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEi PS51175. CBM6. 2 hits.
PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the Clostridium stercorarium xynA gene encoding xylanase A: identification of catalytic and cellulose binding domains."
    Sakka K., Kojima Y., Kondo T., Karita S., Ohmiya K., Shimada K.
    Biosci. Biotechnol. Biochem. 57:273-277(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-40.
    Strain: F-9.
  2. Sakka K.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiXYNA2_CLOSR
AccessioniPrimary (citable) accession number: P33558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3