Reviewed, UniProtKB/Swiss-Prot P33550 (KTR2_YEAST)

Names and origin

Protein names

Recommended name:
    Probable mannosyltransferase KTR2
    EC=2.4.1.131

Gene names

Name:	KTR2
Ordered Locus Names:	YKR061W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in N-linked glycosylation.
Catalytic activity	Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the glycosyltransferase 15 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall mannoprotein biosynthetic process Inferred from mutant phenotype. Source: SGD protein amino acid N-linked glycosylation Inferred from mutant phenotype. Source: SGD
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycolipid 2-alpha-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Probable mannosyltransferase KTR2		PRO_0000208243
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 33	20	Signal-anchor for type II membrane protein
	Topological domain	34 – 425	392	Lumenal Potential
	Region	34 – 89	56	Stem region By similarity
	Region	90 – 425	336	Catalytic By similarity
Sites
	Active site	313	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	65	1	N-linked (GlcNAc...) Potential
	Glycosylation	81	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P33550-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: CF48E73E5BB0F832	FASTA	425	50,651
	10 20 30 40 50 60 MQICKVFLTQ VKKLLFVSLL FCLIAQTCWL ALVPYQRQLS LDSYFFRRSR EVSSRYDFTR 70 80 90 100 110 120 RRHMNQTLKL SSNTYNDEPL NKTKGIKNQR ENATLLMLVR NWELSGALRS MRSLEDRFNK 130 140 150 160 170 180 NYQYDWTFLN DVPFDQEFIE ATTAMASGRT QYALIPAEDW NRPSWINETL FEEALQLMEE 190 200 210 220 230 240 KNILYGGSKS YRNMCRFNSG FFFRQKILDP YDFYFRVEPD VEYFCDFPYD PFKVMRQNNK 250 260 270 280 290 300 KYGFVITMYE YEDTIPSLWE AVEEYLEETE SADIDMESNA FGFVSNFDFI GKSFGVIDSN 310 320 330 340 350 360 SGYNLCHFWT NFEIGDLNFF RSEKYIRFFE YLDSKGGFYY ERWGDAPVHS IAASLLLKKD 370 380 390 400 410 420 EIIHFDELGY KHMPFGTCPS AYYLRLQQRC LCDSNHPDNI DLNVISCLRR WWKDGSGKYF LKHDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"KTR2: a new member of the KRE2 mannosyltransferase gene family." Lussier M., Camirand A., Sdicu A.-M., Bussey H. Yeast 9:1057-1063(1993) [PubMed: 8256512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L17083 Unassigned DNA. Translation: AAA16119.1. Z28286 Genomic DNA. Translation: CAA82140.1.
PIR	S38193.
RefSeq	NP_012987.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT15. Glycosyltransferase Family 15.
Genome annotation databases
Ensembl	YKR061W. Saccharomyces cerevisiae. [Contig view]
GeneID	853935.
GenomeReviews	Gene locus YKR061W in contig Y13137_GR.
KEGG	sce:YKR061W.
NMPDR	fig|4932.3.peg.3973.
Organism-specific databases
CYGD	YKR061w.
SGD	S000001769. KTR2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P33550.
OMA	P33550. ENATMVM.
Enzyme and pathway databases
BRENDA	2.4.1.131. 250.
Gene expression databases
GermOnline	YKR061W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002685. Glyco_trans_15. [Graphical view]
Pfam	PF01793. Glyco_transf_15. 1 hit. [Graphical view]
PIRSF	PIRSF018153. Glyco_trans_15. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	975312.

Entry information

Entry name

KTR2_YEAST

Accession

Primary (citable) accession number: P33550

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)