ID   OPRK_MOUSE              Reviewed;         380 AA.
AC   P33534;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   24-JAN-2024, entry version 183.
DE   RecName: Full=Kappa-type opioid receptor;
DE            Short=K-OR-1;
DE            Short=KOR-1;
DE   AltName: Full=MSL-1;
GN   Name=Oprk1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RC   TISSUE=Brain;
RX   PubMed=8393575; DOI=10.1073/pnas.90.14.6736;
RA   Yasuda K., Raynor K., Kong H., Breder C.D., Takeda J., Reisine T.,
RA   Bell G.I.;
RT   "Cloning and functional comparison of kappa and delta opioid receptors from
RT   mouse brain.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:6736-6740(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7802669; DOI=10.1006/bbrc.1994.2814;
RA   Nishi M., Takeshima H., Mori M., Nakagawara K., Takeuchi T.;
RT   "Structure and chromosomal mapping of genes for the mouse kappa-opioid
RT   receptor and an opioid receptor homologue (MOR-C).";
RL   Biochem. Biophys. Res. Commun. 205:1353-1357(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7733933; DOI=10.1006/bbrc.1995.1547;
RA   Liu H.C., Lu S., Augustin L.B., Felsheim R.F., Chen H.C., Loh H.H.,
RA   Wei L.N.;
RT   "Cloning and promoter mapping of mouse kappa opioid receptor gene.";
RL   Biochem. Biophys. Res. Commun. 209:639-647(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7499487; DOI=10.1016/0165-5728(95)00116-j;
RA   Belkowski S.M., Zhu J., Liu-Chen L.Y., Eisenstein T.K., Adler M.W.,
RA   Rogers T.J.;
RT   "Sequence of kappa-opioid receptor cDNA in the R1.1 thymoma cell line.";
RL   J. Neuroimmunol. 62:113-117(1995).
RN   [5]
RP   DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=9463367; DOI=10.1093/emboj/17.4.886;
RA   Simonin F., Valverde O., Smadja C., Slowe S., Kitchen I., Dierich A.,
RA   Le Meur M., Roques B.P., Maldonado R., Kieffer B.L.;
RT   "Disruption of the kappa-opioid receptor gene in mice enhances sensitivity
RT   to chemical visceral pain, impairs pharmacological actions of the selective
RT   kappa-agonist U-50,488H and attenuates morphine withdrawal.";
RL   EMBO J. 17:886-897(1998).
CC   -!- FUNCTION: G-protein coupled opioid receptor that functions as a
CC       receptor for endogenous alpha-neoendorphins and dynorphins, but has low
CC       affinity for beta-endorphins. Also functions as a receptor for various
CC       synthetic opioids and for the psychoactive diterpene salvinorin A.
CC       Ligand binding causes a conformation change that triggers signaling via
CC       guanine nucleotide-binding proteins (G proteins) and modulates the
CC       activity of down-stream effectors, such as adenylate cyclase. Signaling
CC       leads to the inhibition of adenylate cyclase activity. Inhibits
CC       neurotransmitter release by reducing calcium ion currents and
CC       increasing potassium ion conductance. Plays a role in the perception of
CC       pain. Plays a role in mediating reduced physical activity upon
CC       treatment with synthetic opioids. Plays a role in the regulation of
CC       salivation in response to synthetic opioids. May play a role in arousal
CC       and regulation of autonomic and neuroendocrine functions.
CC       {ECO:0000269|PubMed:8393575, ECO:0000269|PubMed:9463367}.
CC   -!- SUBUNIT: Interacts with NHERF1. Interacts with GABARAPL1 (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:8393575,
CC       ECO:0000269|PubMed:9463367}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:8393575, ECO:0000269|PubMed:9463367}.
CC   -!- TISSUE SPECIFICITY: Detected in brain (at protein level). Brain
CC       (neocortex, hippocampus, amygdala, medial habenula, hypothalamus, locus
CC       ceruleus, and parabrachial nucleus). {ECO:0000269|PubMed:8393575,
CC       ECO:0000269|PubMed:9463367}.
CC   -!- DISRUPTION PHENOTYPE: Mice are born at the expected Mendelian rate and
CC       show no obvious phenotype. Mutant mice do not display analgesia after
CC       treatment with the synthetic agonist U-50,488H. Unlike wild-type mice,
CC       they do not show reduced locomotion and increased salivation in
CC       response to the synthetic agonist U-50,488H.
CC       {ECO:0000269|PubMed:9463367}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR   EMBL; L11065; AAA39363.1; -; mRNA.
DR   EMBL; D31665; BAA06508.1; -; Genomic_DNA.
DR   EMBL; S77872; AAB34130.2; -; Genomic_DNA.
DR   EMBL; S77868; AAB34130.2; JOINED; Genomic_DNA.
DR   EMBL; S77869; AAB34130.2; JOINED; Genomic_DNA.
DR   EMBL; S81111; AAP32232.1; -; mRNA.
DR   CCDS; CCDS14809.1; -.
DR   PIR; A48227; A48227.
DR   PIR; JC2434; JC2434.
DR   RefSeq; NP_001191300.1; NM_001204371.1.
DR   RefSeq; NP_001305664.1; NM_001318735.1.
DR   RefSeq; NP_035141.1; NM_011011.2.
DR   AlphaFoldDB; P33534; -.
DR   SMR; P33534; -.
DR   STRING; 10090.ENSMUSP00000125105; -.
DR   BindingDB; P33534; -.
DR   ChEMBL; CHEMBL4329; -.
DR   DrugCentral; P33534; -.
DR   GuidetoPHARMACOLOGY; 318; -.
DR   GlyCosmos; P33534; 2 sites, No reported glycans.
DR   GlyGen; P33534; 2 sites.
DR   iPTMnet; P33534; -.
DR   PhosphoSitePlus; P33534; -.
DR   SwissPalm; P33534; -.
DR   PaxDb; 10090-ENSMUSP00000027038; -.
DR   Antibodypedia; 11640; 452 antibodies from 38 providers.
DR   DNASU; 18387; -.
DR   Ensembl; ENSMUST00000027038.11; ENSMUSP00000027038.5; ENSMUSG00000025905.15.
DR   Ensembl; ENSMUST00000160339.2; ENSMUSP00000124030.2; ENSMUSG00000025905.15.
DR   Ensembl; ENSMUST00000160777.8; ENSMUSP00000125105.2; ENSMUSG00000025905.15.
DR   GeneID; 18387; -.
DR   KEGG; mmu:18387; -.
DR   UCSC; uc007afo.2; mouse.
DR   AGR; MGI:97439; -.
DR   CTD; 4986; -.
DR   MGI; MGI:97439; Oprk1.
DR   VEuPathDB; HostDB:ENSMUSG00000025905; -.
DR   eggNOG; KOG3656; Eukaryota.
DR   GeneTree; ENSGT00940000157341; -.
DR   HOGENOM; CLU_009579_8_1_1; -.
DR   InParanoid; P33534; -.
DR   OMA; QDPTYMR; -.
DR   OrthoDB; 5393360at2759; -.
DR   PhylomeDB; P33534; -.
DR   TreeFam; TF315737; -.
DR   Reactome; R-MMU-375276; Peptide ligand-binding receptors.
DR   Reactome; R-MMU-418594; G alpha (i) signalling events.
DR   BioGRID-ORCS; 18387; 3 hits in 78 CRISPR screens.
DR   PRO; PR:P33534; -.
DR   Proteomes; UP000000589; Chromosome 1.
DR   RNAct; P33534; Protein.
DR   Bgee; ENSMUSG00000025905; Expressed in decidua basalis and 77 other cell types or tissues.
DR   ExpressionAtlas; P33534; baseline and differential.
DR   GO; GO:0043679; C:axon terminus; ISO:MGI.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0030425; C:dendrite; ISO:MGI.
DR   GO; GO:0016020; C:membrane; ISS:UniProtKB.
DR   GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR   GO; GO:0043025; C:neuronal cell body; ISO:MGI.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0043204; C:perikaryon; ISO:MGI.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   GO; GO:0045211; C:postsynaptic membrane; ISO:MGI.
DR   GO; GO:0042734; C:presynaptic membrane; ISO:MGI.
DR   GO; GO:0030672; C:synaptic vesicle membrane; ISO:MGI.
DR   GO; GO:0038048; F:dynorphin receptor activity; ISS:UniProtKB.
DR   GO; GO:0004985; F:G protein-coupled opioid receptor activity; IDA:UniProtKB.
DR   GO; GO:0042923; F:neuropeptide binding; IBA:GO_Central.
DR   GO; GO:0033612; F:receptor serine/threonine kinase binding; ISO:MGI.
DR   GO; GO:0031635; P:adenylate cyclase-inhibiting opioid receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0048148; P:behavioral response to cocaine; ISO:MGI.
DR   GO; GO:0071333; P:cellular response to glucose stimulus; ISO:MGI.
DR   GO; GO:1990708; P:conditioned place preference; ISO:MGI.
DR   GO; GO:0051607; P:defense response to virus; ISO:MGI.
DR   GO; GO:0042755; P:eating behavior; ISO:MGI.
DR   GO; GO:0038003; P:G protein-coupled opioid receptor signaling pathway; ISO:MGI.
DR   GO; GO:0006955; P:immune response; ISO:MGI.
DR   GO; GO:0007626; P:locomotory behavior; IMP:UniProtKB.
DR   GO; GO:0042711; P:maternal behavior; ISO:MGI.
DR   GO; GO:0033685; P:negative regulation of luteinizing hormone secretion; ISO:MGI.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR   GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0033603; P:positive regulation of dopamine secretion; ISO:MGI.
DR   GO; GO:1904000; P:positive regulation of eating behavior; ISO:MGI.
DR   GO; GO:1900745; P:positive regulation of p38MAPK cascade; ISO:MGI.
DR   GO; GO:1901381; P:positive regulation of potassium ion transmembrane transport; ISO:MGI.
DR   GO; GO:0046877; P:regulation of saliva secretion; IMP:UniProtKB.
DR   GO; GO:0032868; P:response to insulin; ISO:MGI.
DR   GO; GO:0019233; P:sensory perception of pain; IMP:UniProtKB.
DR   GO; GO:0050951; P:sensory perception of temperature stimulus; ISO:MGI.
DR   CDD; cd15091; 7tmA_Kappa_opioid_R; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   InterPro; IPR000452; Kappa_opi_rcpt.
DR   InterPro; IPR001418; Opioid_rcpt.
DR   PANTHER; PTHR24229:SF1; KAPPA-TYPE OPIOID RECEPTOR; 1.
DR   PANTHER; PTHR24229; NEUROPEPTIDES RECEPTOR; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   PRINTS; PR00532; KAPPAOPIOIDR.
DR   PRINTS; PR00384; OPIOIDR.
DR   SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR   Genevisible; P33534; MM.
PE   1: Evidence at protein level;
KW   Behavior; Cell membrane; Disulfide bond; G-protein coupled receptor;
KW   Glycoprotein; Lipoprotein; Membrane; Palmitate; Receptor;
KW   Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT   CHAIN           1..380
FT                   /note="Kappa-type opioid receptor"
FT                   /id="PRO_0000069968"
FT   TOPO_DOM        1..57
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        58..85
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        86..95
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        96..119
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        120..132
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        133..154
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        155..173
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        174..196
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        197..222
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        223..247
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        248..274
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        275..296
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        297..311
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        312..333
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        334..380
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   LIPID           345
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        25
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        39
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000250"
FT   DISULFID        131..210
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT   CONFLICT        211
FT                   /note="S -> L (in Ref. 2; BAA06508)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        231
FT                   /note="F -> V (in Ref. 2; BAA06508)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   380 AA;  42652 MW;  FA4FC947D4545318 CRC64;
     MESPIQIFRG DPGPTCSPSA CLLPNSSSWF PNWAESDSNG SVGSEDQQLE SAHISPAIPV
     IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN LALADALVTT TMPFQSAVYL
     MNSWPFGDVL CKIVISIDYY NMFTSIFTLT MMSVDRYIAV CHPVKALDFR TPLKAKIINI
     CIWLLASSVG ISAIVLGGTK VREDVDVIEC SLQFPDDEYS WWDLFMKICV FVFAFVIPVL
     IIIVCYTLMI LRLKSVRLLS GSREKDRNLR RITKLVLVVV AVFIICWTPI HIFILVEALG
     STSHSTAALS SYYFCIALGY TNSSLNPVLY AFLDENFKRC FRDFCFPIKM RMERQSTNRV
     RNTVQDPASM RDVGGMNKPV
//