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P33534

- OPRK_MOUSE

UniProt

P33534 - OPRK_MOUSE

Protein

Kappa-type opioid receptor

Gene

Oprk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.2 Publications

    GO - Molecular functioni

    1. dynorphin receptor activity Source: UniProtKB
    2. opioid receptor activity Source: UniProtKB

    GO - Biological processi

    1. adenylate cyclase-inhibiting opioid receptor signaling pathway Source: UniProtKB
    2. defense response to virus Source: Ensembl
    3. immune response Source: Ensembl
    4. locomotory behavior Source: UniProtKB
    5. phospholipase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
    6. regulation of saliva secretion Source: UniProtKB
    7. sensory perception of pain Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Behavior

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kappa-type opioid receptor
    Short name:
    K-OR-1
    Short name:
    KOR-1
    Alternative name(s):
    MSL-1
    Gene namesi
    Name:Oprk1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:97439. Oprk1.

    Subcellular locationi

    Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB
    2. integral component of plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice are born at the expected Mendelian rate and show no obvious phenotype. Mutant mice do not display analgesia after treatment with the synthetic agonist U-50,488H. Unlike wild-type mice, they do not show reduced locomotion and increased salivation in response to the synthetic agonist U-50,488H.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 380380Kappa-type opioid receptorPRO_0000069968Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi25 – 251N-linked (GlcNAc...)By similarity
    Glycosylationi39 – 391N-linked (GlcNAc...)By similarity
    Disulfide bondi131 ↔ 210PROSITE-ProRule annotation
    Lipidationi345 – 3451S-palmitoyl cysteineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PRIDEiP33534.

    PTM databases

    PhosphoSiteiP33534.

    Expressioni

    Tissue specificityi

    Detected in brain (at protein level). Brain (neocortex, hippocampus, amygdala, medial habenula, hypothalamus, locus ceruleus, and parabrachial nucleus).2 Publications

    Gene expression databases

    ArrayExpressiP33534.
    BgeeiP33534.
    CleanExiMM_OPRK1.
    GenevestigatoriP33534.

    Interactioni

    Subunit structurei

    Interacts with SLC9A3R1. Interacts with GABARAPL1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP33534.
    SMRiP33534. Positions 55-347.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5757ExtracellularBy similarityAdd
    BLAST
    Topological domaini86 – 9510CytoplasmicBy similarity
    Topological domaini120 – 13213ExtracellularBy similarityAdd
    BLAST
    Topological domaini155 – 17319CytoplasmicBy similarityAdd
    BLAST
    Topological domaini197 – 22226ExtracellularBy similarityAdd
    BLAST
    Topological domaini248 – 27427CytoplasmicBy similarityAdd
    BLAST
    Topological domaini297 – 31115ExtracellularBy similarityAdd
    BLAST
    Topological domaini334 – 38047CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei58 – 8528Helical; Name=1By similarityAdd
    BLAST
    Transmembranei96 – 11924Helical; Name=2By similarityAdd
    BLAST
    Transmembranei133 – 15422Helical; Name=3By similarityAdd
    BLAST
    Transmembranei174 – 19623Helical; Name=4By similarityAdd
    BLAST
    Transmembranei223 – 24725Helical; Name=5By similarityAdd
    BLAST
    Transmembranei275 – 29622Helical; Name=6By similarityAdd
    BLAST
    Transmembranei312 – 33322Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG317066.
    HOGENOMiHOG000230486.
    HOVERGENiHBG106919.
    InParanoidiP33534.
    KOiK04214.
    OMAiPFQSTEY.
    OrthoDBiEOG7BKCVQ.
    PhylomeDBiP33534.
    TreeFamiTF315737.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR000452. Kappa_opi_rcpt.
    IPR001418. Opioid_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00532. KAPPAOPIOIDR.
    PR00384. OPIOIDR.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33534-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESPIQIFRG DPGPTCSPSA CLLPNSSSWF PNWAESDSNG SVGSEDQQLE    50
    SAHISPAIPV IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN 100
    LALADALVTT TMPFQSAVYL MNSWPFGDVL CKIVISIDYY NMFTSIFTLT 150
    MMSVDRYIAV CHPVKALDFR TPLKAKIINI CIWLLASSVG ISAIVLGGTK 200
    VREDVDVIEC SLQFPDDEYS WWDLFMKICV FVFAFVIPVL IIIVCYTLMI 250
    LRLKSVRLLS GSREKDRNLR RITKLVLVVV AVFIICWTPI HIFILVEALG 300
    STSHSTAALS SYYFCIALGY TNSSLNPVLY AFLDENFKRC FRDFCFPIKM 350
    RMERQSTNRV RNTVQDPASM RDVGGMNKPV 380
    Length:380
    Mass (Da):42,652
    Last modified:February 1, 1994 - v1
    Checksum:iFA4FC947D4545318
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti211 – 2111S → L in BAA06508. (PubMed:7802669)Curated
    Sequence conflicti231 – 2311F → V in BAA06508. (PubMed:7802669)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11065 mRNA. Translation: AAA39363.1.
    D31665 Genomic DNA. Translation: BAA06508.1.
    S77872, S77868, S77869 Genomic DNA. Translation: AAB34130.2.
    S81111 mRNA. Translation: AAP32232.1.
    CCDSiCCDS14809.1.
    PIRiA48227.
    JC2434.
    RefSeqiNP_001191300.1. NM_001204371.1.
    NP_035141.1. NM_011011.2.
    UniGeneiMm.7977.

    Genome annotation databases

    EnsembliENSMUST00000027038; ENSMUSP00000027038; ENSMUSG00000025905.
    ENSMUST00000160339; ENSMUSP00000124030; ENSMUSG00000025905.
    ENSMUST00000160777; ENSMUSP00000125105; ENSMUSG00000025905.
    GeneIDi18387.
    KEGGimmu:18387.
    UCSCiuc007afo.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11065 mRNA. Translation: AAA39363.1 .
    D31665 Genomic DNA. Translation: BAA06508.1 .
    S77872 , S77868 , S77869 Genomic DNA. Translation: AAB34130.2 .
    S81111 mRNA. Translation: AAP32232.1 .
    CCDSi CCDS14809.1.
    PIRi A48227.
    JC2434.
    RefSeqi NP_001191300.1. NM_001204371.1.
    NP_035141.1. NM_011011.2.
    UniGenei Mm.7977.

    3D structure databases

    ProteinModelPortali P33534.
    SMRi P33534. Positions 55-347.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P33534.
    ChEMBLi CHEMBL2095192.
    GuidetoPHARMACOLOGYi 318.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P33534.

    Proteomic databases

    PRIDEi P33534.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027038 ; ENSMUSP00000027038 ; ENSMUSG00000025905 .
    ENSMUST00000160339 ; ENSMUSP00000124030 ; ENSMUSG00000025905 .
    ENSMUST00000160777 ; ENSMUSP00000125105 ; ENSMUSG00000025905 .
    GeneIDi 18387.
    KEGGi mmu:18387.
    UCSCi uc007afo.2. mouse.

    Organism-specific databases

    CTDi 4986.
    MGIi MGI:97439. Oprk1.

    Phylogenomic databases

    eggNOGi NOG317066.
    HOGENOMi HOG000230486.
    HOVERGENi HBG106919.
    InParanoidi P33534.
    KOi K04214.
    OMAi PFQSTEY.
    OrthoDBi EOG7BKCVQ.
    PhylomeDBi P33534.
    TreeFami TF315737.

    Miscellaneous databases

    NextBioi 293980.
    PROi P33534.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P33534.
    Bgeei P33534.
    CleanExi MM_OPRK1.
    Genevestigatori P33534.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR000452. Kappa_opi_rcpt.
    IPR001418. Opioid_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00532. KAPPAOPIOIDR.
    PR00384. OPIOIDR.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and functional comparison of kappa and delta opioid receptors from mouse brain."
      Yasuda K., Raynor K., Kong H., Breder C.D., Takeda J., Reisine T., Bell G.I.
      Proc. Natl. Acad. Sci. U.S.A. 90:6736-6740(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Brain.
    2. "Structure and chromosomal mapping of genes for the mouse kappa-opioid receptor and an opioid receptor homologue (MOR-C)."
      Nishi M., Takeshima H., Mori M., Nakagawara K., Takeuchi T.
      Biochem. Biophys. Res. Commun. 205:1353-1357(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Sequence of kappa-opioid receptor cDNA in the R1.1 thymoma cell line."
      Belkowski S.M., Zhu J., Liu-Chen L.Y., Eisenstein T.K., Adler M.W., Rogers T.J.
      J. Neuroimmunol. 62:113-117(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "Disruption of the kappa-opioid receptor gene in mice enhances sensitivity to chemical visceral pain, impairs pharmacological actions of the selective kappa-agonist U-50,488H and attenuates morphine withdrawal."
      Simonin F., Valverde O., Smadja C., Slowe S., Kitchen I., Dierich A., Le Meur M., Roques B.P., Maldonado R., Kieffer B.L.
      EMBO J. 17:886-897(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiOPRK_MOUSE
    AccessioniPrimary (citable) accession number: P33534
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3