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Protein

Kappa-type opioid receptor

Gene

Oprk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.2 Publications

GO - Molecular functioni

  • dynorphin receptor activity Source: UniProtKB
  • neuropeptide binding Source: GO_Central
  • opioid receptor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-type opioid receptor
Short name:
K-OR-1
Short name:
KOR-1
Alternative name(s):
MSL-1
Gene namesi
Name:Oprk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:97439. Oprk1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 57ExtracellularBy similarityAdd BLAST57
Transmembranei58 – 85Helical; Name=1By similarityAdd BLAST28
Topological domaini86 – 95CytoplasmicBy similarity10
Transmembranei96 – 119Helical; Name=2By similarityAdd BLAST24
Topological domaini120 – 132ExtracellularBy similarityAdd BLAST13
Transmembranei133 – 154Helical; Name=3By similarityAdd BLAST22
Topological domaini155 – 173CytoplasmicBy similarityAdd BLAST19
Transmembranei174 – 196Helical; Name=4By similarityAdd BLAST23
Topological domaini197 – 222ExtracellularBy similarityAdd BLAST26
Transmembranei223 – 247Helical; Name=5By similarityAdd BLAST25
Topological domaini248 – 274CytoplasmicBy similarityAdd BLAST27
Transmembranei275 – 296Helical; Name=6By similarityAdd BLAST22
Topological domaini297 – 311ExtracellularBy similarityAdd BLAST15
Transmembranei312 – 333Helical; Name=7By similarityAdd BLAST22
Topological domaini334 – 380CytoplasmicBy similarityAdd BLAST47

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate and show no obvious phenotype. Mutant mice do not display analgesia after treatment with the synthetic agonist U-50,488H. Unlike wild-type mice, they do not show reduced locomotion and increased salivation in response to the synthetic agonist U-50,488H.1 Publication

Chemistry databases

ChEMBLiCHEMBL4329.
GuidetoPHARMACOLOGYi318.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699681 – 380Kappa-type opioid receptorAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...)By similarity1
Glycosylationi39N-linked (GlcNAc...)By similarity1
Disulfide bondi131 ↔ 210PROSITE-ProRule annotation
Modified residuei157PhosphotyrosineBy similarity1
Lipidationi345S-palmitoyl cysteineSequence analysis1
Modified residuei363PhosphothreonineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP33534.
PRIDEiP33534.

PTM databases

iPTMnetiP33534.
PhosphoSitePlusiP33534.

Expressioni

Tissue specificityi

Detected in brain (at protein level). Brain (neocortex, hippocampus, amygdala, medial habenula, hypothalamus, locus ceruleus, and parabrachial nucleus).2 Publications

Gene expression databases

BgeeiENSMUSG00000025905.
CleanExiMM_OPRK1.
ExpressionAtlasiP33534. baseline and differential.
GenevisibleiP33534. MM.

Interactioni

Subunit structurei

Interacts with SLC9A3R1. Interacts with GABARAPL1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027038.

Chemistry databases

BindingDBiP33534.

Structurei

3D structure databases

ProteinModelPortaliP33534.
SMRiP33534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP33534.
KOiK04214.
OMAiPFQSTEY.
OrthoDBiEOG091G0HEN.
PhylomeDBiP33534.
TreeFamiTF315737.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESPIQIFRG DPGPTCSPSA CLLPNSSSWF PNWAESDSNG SVGSEDQQLE
60 70 80 90 100
SAHISPAIPV IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN
110 120 130 140 150
LALADALVTT TMPFQSAVYL MNSWPFGDVL CKIVISIDYY NMFTSIFTLT
160 170 180 190 200
MMSVDRYIAV CHPVKALDFR TPLKAKIINI CIWLLASSVG ISAIVLGGTK
210 220 230 240 250
VREDVDVIEC SLQFPDDEYS WWDLFMKICV FVFAFVIPVL IIIVCYTLMI
260 270 280 290 300
LRLKSVRLLS GSREKDRNLR RITKLVLVVV AVFIICWTPI HIFILVEALG
310 320 330 340 350
STSHSTAALS SYYFCIALGY TNSSLNPVLY AFLDENFKRC FRDFCFPIKM
360 370 380
RMERQSTNRV RNTVQDPASM RDVGGMNKPV
Length:380
Mass (Da):42,652
Last modified:February 1, 1994 - v1
Checksum:iFA4FC947D4545318
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211S → L in BAA06508 (PubMed:7802669).Curated1
Sequence conflicti231F → V in BAA06508 (PubMed:7802669).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11065 mRNA. Translation: AAA39363.1.
D31665 Genomic DNA. Translation: BAA06508.1.
S77872, S77868, S77869 Genomic DNA. Translation: AAB34130.2.
S81111 mRNA. Translation: AAP32232.1.
CCDSiCCDS14809.1.
PIRiA48227.
JC2434.
RefSeqiNP_001191300.1. NM_001204371.1.
NP_001305664.1. NM_001318735.1.
NP_035141.1. NM_011011.2.
UniGeneiMm.7977.

Genome annotation databases

EnsembliENSMUST00000027038; ENSMUSP00000027038; ENSMUSG00000025905.
ENSMUST00000160339; ENSMUSP00000124030; ENSMUSG00000025905.
ENSMUST00000160777; ENSMUSP00000125105; ENSMUSG00000025905.
GeneIDi18387.
KEGGimmu:18387.
UCSCiuc007afo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11065 mRNA. Translation: AAA39363.1.
D31665 Genomic DNA. Translation: BAA06508.1.
S77872, S77868, S77869 Genomic DNA. Translation: AAB34130.2.
S81111 mRNA. Translation: AAP32232.1.
CCDSiCCDS14809.1.
PIRiA48227.
JC2434.
RefSeqiNP_001191300.1. NM_001204371.1.
NP_001305664.1. NM_001318735.1.
NP_035141.1. NM_011011.2.
UniGeneiMm.7977.

3D structure databases

ProteinModelPortaliP33534.
SMRiP33534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027038.

Chemistry databases

BindingDBiP33534.
ChEMBLiCHEMBL4329.
GuidetoPHARMACOLOGYi318.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP33534.
PhosphoSitePlusiP33534.

Proteomic databases

PaxDbiP33534.
PRIDEiP33534.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027038; ENSMUSP00000027038; ENSMUSG00000025905.
ENSMUST00000160339; ENSMUSP00000124030; ENSMUSG00000025905.
ENSMUST00000160777; ENSMUSP00000125105; ENSMUSG00000025905.
GeneIDi18387.
KEGGimmu:18387.
UCSCiuc007afo.2. mouse.

Organism-specific databases

CTDi4986.
MGIiMGI:97439. Oprk1.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP33534.
KOiK04214.
OMAiPFQSTEY.
OrthoDBiEOG091G0HEN.
PhylomeDBiP33534.
TreeFamiTF315737.

Enzyme and pathway databases

ReactomeiR-MMU-375276. Peptide ligand-binding receptors.
R-MMU-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiP33534.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025905.
CleanExiMM_OPRK1.
ExpressionAtlasiP33534. baseline and differential.
GenevisibleiP33534. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOPRK_MOUSE
AccessioniPrimary (citable) accession number: P33534
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.