P33534 (OPRK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kappa-type opioid receptor
Short name=K-OR-1
Short name=KOR-1

Alternative name(s):
MSL-1

Gene names

Name:

Oprk1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions.
Subunit structure	Interacts with SLC9A3R1. Interacts with GABARAPL1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Brain (neocortex, hippocampus, amygdala, medial habenula, hypothalamus, locus ceruleus, and parabrachial nucleus).
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to virus Inferred from electronic annotation. Source: Compara immune response Inferred from electronic annotation. Source: Compara phospholipase C-activating G-protein coupled receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	dynorphin receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Kappa-type opioid receptor		PRO_0000069968
Regions
	Topological domain	1 – 58	58	Extracellular Potential
	Transmembrane	59 – 85	27	Helical; Name=1; Potential
	Topological domain	86 – 95	10	Cytoplasmic Potential
	Transmembrane	96 – 117	22	Helical; Name=2; Potential
	Topological domain	118 – 132	15	Extracellular Potential
	Transmembrane	133 – 154	22	Helical; Name=3; Potential
	Topological domain	155 – 173	19	Cytoplasmic Potential
	Transmembrane	174 – 196	23	Helical; Name=4; Potential
	Topological domain	197 – 222	26	Extracellular Potential
	Transmembrane	223 – 247	25	Helical; Name=5; Potential
	Topological domain	248 – 275	28	Cytoplasmic Potential
	Transmembrane	276 – 299	24	Helical; Name=6; Potential
	Topological domain	300 – 311	12	Extracellular Potential
	Transmembrane	312 – 333	22	Helical; Name=7; Potential
	Topological domain	334 – 380	47	Cytoplasmic Potential
Amino acid modifications
	Lipidation	345	1	S-palmitoyl cysteine Potential
	Glycosylation	25	1	N-linked (GlcNAc...) By similarity
	Glycosylation	39	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	131 ↔ 210		By similarity
Experimental info
	Sequence conflict	211	1	S → L Ref.2
	Sequence conflict	211	1	S → L Ref.3
	Sequence conflict	231	1	F → V Ref.2
	Sequence conflict	231	1	F → V Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P33534 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: FA4FC947D4545318	FASTA	380	42,652
	10 20 30 40 50 60 MESPIQIFRG DPGPTCSPSA CLLPNSSSWF PNWAESDSNG SVGSEDQQLE SAHISPAIPV 70 80 90 100 110 120 IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN LALADALVTT TMPFQSAVYL 130 140 150 160 170 180 MNSWPFGDVL CKIVISIDYY NMFTSIFTLT MMSVDRYIAV CHPVKALDFR TPLKAKIINI 190 200 210 220 230 240 CIWLLASSVG ISAIVLGGTK VREDVDVIEC SLQFPDDEYS WWDLFMKICV FVFAFVIPVL 250 260 270 280 290 300 IIIVCYTLMI LRLKSVRLLS GSREKDRNLR RITKLVLVVV AVFIICWTPI HIFILVEALG 310 320 330 340 350 360 STSHSTAALS SYYFCIALGY TNSSLNPVLY AFLDENFKRC FRDFCFPIKM RMERQSTNRV 370 380 RNTVQDPASM RDVGGMNKPV

References

[1]	"Cloning and functional comparison of kappa and delta opioid receptors from mouse brain." Yasuda K., Raynor K., Kong H., Breder C.D., Takeda J., Reisine T., Bell G.I. Proc. Natl. Acad. Sci. U.S.A. 90:6736-6740(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Structure and chromosomal mapping of genes for the mouse kappa-opioid receptor and an opioid receptor homologue (MOR-C)." Nishi M., Takeshima H., Mori M., Nakagawara K., Takeuchi T. Biochem. Biophys. Res. Commun. 205:1353-1357(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning and promoter mapping of mouse kappa opioid receptor gene." Liu H.C., Lu S., Augustin L.B., Felsheim R.F., Chen H.C., Loh H.H., Wei L.N. Biochem. Biophys. Res. Commun. 209:639-647(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Sequence of kappa-opioid receptor cDNA in the R1.1 thymoma cell line." Belkowski S.M., Zhu J., Liu-Chen L.Y., Eisenstein T.K., Adler M.W., Rogers T.J. J. Neuroimmunol. 62:113-117(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L11065 mRNA. Translation: AAA39363.1. D31665 Genomic DNA. Translation: BAA06508.1. S77872, S77868, S77869 Genomic DNA. Translation: AAB34130.2. S81111 mRNA. Translation: AAP32232.1.
IPI	IPI00113663.
PIR	A48227. JC2434.
RefSeq	NP_001191300.1. NM_001204371.1. NP_035141.1. NM_011011.2.
UniGene	Mm.7977.
3D structure databases
ProteinModelPortal	P33534.
SMR	P33534. Positions 55-347.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P33534.
Proteomic databases
PRIDE	P33534.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027038; ENSMUSP00000027038; ENSMUSG00000025905. ENSMUST00000160339; ENSMUSP00000124030; ENSMUSG00000025905. ENSMUST00000160777; ENSMUSP00000125105; ENSMUSG00000025905.
GeneID	18387.
KEGG	mmu:18387.
UCSC	uc007afo.2. mouse.
Organism-specific databases
CTD	4986.
MGI	MGI:97439. Oprk1.
Phylogenomic databases
eggNOG	NOG317066.
HOGENOM	HOG000230486.
HOVERGEN	HBG106919.
InParanoid	P33534.
KO	K04214.
OMA	DVDVIEC.
OrthoDB	EOG4GMTXD.
Gene expression databases
ArrayExpress	P33534.
Bgee	P33534.
CleanEx	MM_OPRK1.
Genevestigator	P33534.
GermOnline	ENSMUSG00000025905. Mus musculus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000452. Kappa_opi_rcpt. IPR001418. Opioid_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00532. KAPPAOPIOIDR. PR00384. OPIOIDR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P33534.
ChEMBL	CHEMBL4329.
NextBio	293980.
SOURCE	Search...

Entry information

Entry name

OPRK_MOUSE

Accession

Primary (citable) accession number: P33534

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 29, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program