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P33534 (OPRK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kappa-type opioid receptor

Short name=K-OR-1
Short name=KOR-1
Alternative name(s):
MSL-1
Gene names
Name:Oprk1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length380 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions. Ref.1 Ref.5

Subunit structure

Interacts with SLC9A3R1. Interacts with GABARAPL1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.1 Ref.5.

Tissue specificity

Detected in brain (at protein level). Brain (neocortex, hippocampus, amygdala, medial habenula, hypothalamus, locus ceruleus, and parabrachial nucleus). Ref.1 Ref.5

Disruption phenotype

Mice are born at the expected Mendelian rate and show no obvious phenotype. Mutant mice do not display analgesia after treatment with the synthetic agonist U-50,488H. Unlike wild-type mice, they do not show reduced locomotion and increased salivation in response to the synthetic agonist U-50,488H. Ref.5

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 380380Kappa-type opioid receptor
PRO_0000069968

Regions

Topological domain1 – 5757Extracellular By similarity
Transmembrane58 – 8528Helical; Name=1; By similarity
Topological domain86 – 9510Cytoplasmic By similarity
Transmembrane96 – 11924Helical; Name=2; By similarity
Topological domain120 – 13213Extracellular By similarity
Transmembrane133 – 15422Helical; Name=3; By similarity
Topological domain155 – 17319Cytoplasmic By similarity
Transmembrane174 – 19623Helical; Name=4; By similarity
Topological domain197 – 22226Extracellular By similarity
Transmembrane223 – 24725Helical; Name=5; By similarity
Topological domain248 – 27427Cytoplasmic By similarity
Transmembrane275 – 29622Helical; Name=6; By similarity
Topological domain297 – 31115Extracellular By similarity
Transmembrane312 – 33322Helical; Name=7; By similarity
Topological domain334 – 38047Cytoplasmic By similarity

Amino acid modifications

Lipidation3451S-palmitoyl cysteine Potential
Glycosylation251N-linked (GlcNAc...) By similarity
Glycosylation391N-linked (GlcNAc...) By similarity
Disulfide bond131 ↔ 210 By similarity

Experimental info

Sequence conflict2111S → L in BAA06508. Ref.2
Sequence conflict2311F → V in BAA06508. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P33534 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: FA4FC947D4545318

FASTA38042,652
        10         20         30         40         50         60 
MESPIQIFRG DPGPTCSPSA CLLPNSSSWF PNWAESDSNG SVGSEDQQLE SAHISPAIPV 

        70         80         90        100        110        120 
IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN LALADALVTT TMPFQSAVYL 

       130        140        150        160        170        180 
MNSWPFGDVL CKIVISIDYY NMFTSIFTLT MMSVDRYIAV CHPVKALDFR TPLKAKIINI 

       190        200        210        220        230        240 
CIWLLASSVG ISAIVLGGTK VREDVDVIEC SLQFPDDEYS WWDLFMKICV FVFAFVIPVL 

       250        260        270        280        290        300 
IIIVCYTLMI LRLKSVRLLS GSREKDRNLR RITKLVLVVV AVFIICWTPI HIFILVEALG 

       310        320        330        340        350        360 
STSHSTAALS SYYFCIALGY TNSSLNPVLY AFLDENFKRC FRDFCFPIKM RMERQSTNRV 

       370        380 
RNTVQDPASM RDVGGMNKPV 

« Hide

References

[1]"Cloning and functional comparison of kappa and delta opioid receptors from mouse brain."
Yasuda K., Raynor K., Kong H., Breder C.D., Takeda J., Reisine T., Bell G.I.
Proc. Natl. Acad. Sci. U.S.A. 90:6736-6740(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Brain.
[2]"Structure and chromosomal mapping of genes for the mouse kappa-opioid receptor and an opioid receptor homologue (MOR-C)."
Nishi M., Takeshima H., Mori M., Nakagawara K., Takeuchi T.
Biochem. Biophys. Res. Commun. 205:1353-1357(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Cloning and promoter mapping of mouse kappa opioid receptor gene."
Liu H.C., Lu S., Augustin L.B., Felsheim R.F., Chen H.C., Loh H.H., Wei L.N.
Biochem. Biophys. Res. Commun. 209:639-647(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Sequence of kappa-opioid receptor cDNA in the R1.1 thymoma cell line."
Belkowski S.M., Zhu J., Liu-Chen L.Y., Eisenstein T.K., Adler M.W., Rogers T.J.
J. Neuroimmunol. 62:113-117(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"Disruption of the kappa-opioid receptor gene in mice enhances sensitivity to chemical visceral pain, impairs pharmacological actions of the selective kappa-agonist U-50,488H and attenuates morphine withdrawal."
Simonin F., Valverde O., Smadja C., Slowe S., Kitchen I., Dierich A., Le Meur M., Roques B.P., Maldonado R., Kieffer B.L.
EMBO J. 17:886-897(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L11065 mRNA. Translation: AAA39363.1.
D31665 Genomic DNA. Translation: BAA06508.1.
S77872, S77868, S77869 Genomic DNA. Translation: AAB34130.2.
S81111 mRNA. Translation: AAP32232.1.
CCDSCCDS14809.1.
PIRA48227.
JC2434.
RefSeqNP_001191300.1. NM_001204371.1.
NP_035141.1. NM_011011.2.
UniGeneMm.7977.

3D structure databases

ProteinModelPortalP33534.
SMRP33534. Positions 55-347.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP33534.
ChEMBLCHEMBL2095192.
GuidetoPHARMACOLOGY318.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP33534.

Proteomic databases

PRIDEP33534.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027038; ENSMUSP00000027038; ENSMUSG00000025905.
ENSMUST00000160339; ENSMUSP00000124030; ENSMUSG00000025905.
ENSMUST00000160777; ENSMUSP00000125105; ENSMUSG00000025905.
GeneID18387.
KEGGmmu:18387.
UCSCuc007afo.2. mouse.

Organism-specific databases

CTD4986.
MGIMGI:97439. Oprk1.

Phylogenomic databases

eggNOGNOG317066.
HOGENOMHOG000230486.
HOVERGENHBG106919.
InParanoidP33534.
KOK04214.
OMAPFQSTEY.
OrthoDBEOG7BKCVQ.
PhylomeDBP33534.
TreeFamTF315737.

Gene expression databases

ArrayExpressP33534.
BgeeP33534.
CleanExMM_OPRK1.
GenevestigatorP33534.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio293980.
PROP33534.
SOURCESearch...

Entry information

Entry nameOPRK_MOUSE
AccessionPrimary (citable) accession number: P33534
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries