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Protein

Kappa-type opioid receptor

Gene

Oprk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.2 Publications

GO - Molecular functioni

  • dynorphin receptor activity Source: UniProtKB
  • neuropeptide binding Source: GO_Central
  • opioid receptor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processBehavior

Enzyme and pathway databases

ReactomeiR-MMU-375276 Peptide ligand-binding receptors
R-MMU-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-type opioid receptor
Short name:
K-OR-1
Short name:
KOR-1
Alternative name(s):
MSL-1
Gene namesi
Name:Oprk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:97439 Oprk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 57ExtracellularBy similarityAdd BLAST57
Transmembranei58 – 85Helical; Name=1By similarityAdd BLAST28
Topological domaini86 – 95CytoplasmicBy similarity10
Transmembranei96 – 119Helical; Name=2By similarityAdd BLAST24
Topological domaini120 – 132ExtracellularBy similarityAdd BLAST13
Transmembranei133 – 154Helical; Name=3By similarityAdd BLAST22
Topological domaini155 – 173CytoplasmicBy similarityAdd BLAST19
Transmembranei174 – 196Helical; Name=4By similarityAdd BLAST23
Topological domaini197 – 222ExtracellularBy similarityAdd BLAST26
Transmembranei223 – 247Helical; Name=5By similarityAdd BLAST25
Topological domaini248 – 274CytoplasmicBy similarityAdd BLAST27
Transmembranei275 – 296Helical; Name=6By similarityAdd BLAST22
Topological domaini297 – 311ExtracellularBy similarityAdd BLAST15
Transmembranei312 – 333Helical; Name=7By similarityAdd BLAST22
Topological domaini334 – 380CytoplasmicBy similarityAdd BLAST47

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate and show no obvious phenotype. Mutant mice do not display analgesia after treatment with the synthetic agonist U-50,488H. Unlike wild-type mice, they do not show reduced locomotion and increased salivation in response to the synthetic agonist U-50,488H.1 Publication

Chemistry databases

ChEMBLiCHEMBL4329
GuidetoPHARMACOLOGYi318

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699681 – 380Kappa-type opioid receptorAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi39N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi131 ↔ 210PROSITE-ProRule annotation
Lipidationi345S-palmitoyl cysteineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP33534
PRIDEiP33534

PTM databases

iPTMnetiP33534
PhosphoSitePlusiP33534
SwissPalmiP33534

Expressioni

Tissue specificityi

Detected in brain (at protein level). Brain (neocortex, hippocampus, amygdala, medial habenula, hypothalamus, locus ceruleus, and parabrachial nucleus).2 Publications

Gene expression databases

BgeeiENSMUSG00000025905
CleanExiMM_OPRK1
ExpressionAtlasiP33534 baseline and differential
GenevisibleiP33534 MM

Interactioni

Subunit structurei

Interacts with SLC9A3R1. Interacts with GABARAPL1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027038

Chemistry databases

BindingDBiP33534

Structurei

3D structure databases

ProteinModelPortaliP33534
SMRiP33534
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118797
HOGENOMiHOG000230486
HOVERGENiHBG106919
InParanoidiP33534
KOiK04214
OMAiVRNTVQD
OrthoDBiEOG091G0HEN
PhylomeDBiP33534
TreeFamiTF315737

Family and domain databases

CDDicd15091 7tmA_Kappa_opioid_R, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR000452 Kappa_opi_rcpt
IPR001418 Opioid_rcpt
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00532 KAPPAOPIOIDR
PR00384 OPIOIDR
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P33534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESPIQIFRG DPGPTCSPSA CLLPNSSSWF PNWAESDSNG SVGSEDQQLE
60 70 80 90 100
SAHISPAIPV IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN
110 120 130 140 150
LALADALVTT TMPFQSAVYL MNSWPFGDVL CKIVISIDYY NMFTSIFTLT
160 170 180 190 200
MMSVDRYIAV CHPVKALDFR TPLKAKIINI CIWLLASSVG ISAIVLGGTK
210 220 230 240 250
VREDVDVIEC SLQFPDDEYS WWDLFMKICV FVFAFVIPVL IIIVCYTLMI
260 270 280 290 300
LRLKSVRLLS GSREKDRNLR RITKLVLVVV AVFIICWTPI HIFILVEALG
310 320 330 340 350
STSHSTAALS SYYFCIALGY TNSSLNPVLY AFLDENFKRC FRDFCFPIKM
360 370 380
RMERQSTNRV RNTVQDPASM RDVGGMNKPV
Length:380
Mass (Da):42,652
Last modified:February 1, 1994 - v1
Checksum:iFA4FC947D4545318
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211S → L in BAA06508 (PubMed:7802669).Curated1
Sequence conflicti231F → V in BAA06508 (PubMed:7802669).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11065 mRNA Translation: AAA39363.1
D31665 Genomic DNA Translation: BAA06508.1
S77872, S77868, S77869 Genomic DNA Translation: AAB34130.2
S81111 mRNA Translation: AAP32232.1
CCDSiCCDS14809.1
PIRiA48227
JC2434
RefSeqiNP_001191300.1, NM_001204371.1
NP_001305664.1, NM_001318735.1
NP_035141.1, NM_011011.2
UniGeneiMm.7977

Genome annotation databases

EnsembliENSMUST00000027038; ENSMUSP00000027038; ENSMUSG00000025905
ENSMUST00000160339; ENSMUSP00000124030; ENSMUSG00000025905
ENSMUST00000160777; ENSMUSP00000125105; ENSMUSG00000025905
GeneIDi18387
KEGGimmu:18387
UCSCiuc007afo.2 mouse

Similar proteinsi

Entry informationi

Entry nameiOPRK_MOUSE
AccessioniPrimary (citable) accession number: P33534
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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