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P33534

- OPRK_MOUSE

UniProt

P33534 - OPRK_MOUSE

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Protein

Kappa-type opioid receptor

Gene
Oprk1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.2 Publications

GO - Molecular functioni

  1. dynorphin receptor activity Source: UniProtKB
  2. opioid receptor activity Source: UniProtKB

GO - Biological processi

  1. adenylate cyclase-inhibiting opioid receptor signaling pathway Source: UniProtKB
  2. defense response to virus Source: Ensembl
  3. immune response Source: Ensembl
  4. locomotory behavior Source: UniProtKB
  5. phospholipase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
  6. regulation of saliva secretion Source: UniProtKB
  7. sensory perception of pain Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-type opioid receptor
Short name:
K-OR-1
Short name:
KOR-1
Alternative name(s):
MSL-1
Gene namesi
Name:Oprk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:97439. Oprk1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5757Extracellular By similarityAdd
BLAST
Transmembranei58 – 8528Helical; Name=1; By similarityAdd
BLAST
Topological domaini86 – 9510Cytoplasmic By similarity
Transmembranei96 – 11924Helical; Name=2; By similarityAdd
BLAST
Topological domaini120 – 13213Extracellular By similarityAdd
BLAST
Transmembranei133 – 15422Helical; Name=3; By similarityAdd
BLAST
Topological domaini155 – 17319Cytoplasmic By similarityAdd
BLAST
Transmembranei174 – 19623Helical; Name=4; By similarityAdd
BLAST
Topological domaini197 – 22226Extracellular By similarityAdd
BLAST
Transmembranei223 – 24725Helical; Name=5; By similarityAdd
BLAST
Topological domaini248 – 27427Cytoplasmic By similarityAdd
BLAST
Transmembranei275 – 29622Helical; Name=6; By similarityAdd
BLAST
Topological domaini297 – 31115Extracellular By similarityAdd
BLAST
Transmembranei312 – 33322Helical; Name=7; By similarityAdd
BLAST
Topological domaini334 – 38047Cytoplasmic By similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB
  2. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate and show no obvious phenotype. Mutant mice do not display analgesia after treatment with the synthetic agonist U-50,488H. Unlike wild-type mice, they do not show reduced locomotion and increased salivation in response to the synthetic agonist U-50,488H.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Kappa-type opioid receptorPRO_0000069968Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi25 – 251N-linked (GlcNAc...) By similarity
Glycosylationi39 – 391N-linked (GlcNAc...) By similarity
Disulfide bondi131 ↔ 210 By similarity
Lipidationi345 – 3451S-palmitoyl cysteine Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PRIDEiP33534.

PTM databases

PhosphoSiteiP33534.

Expressioni

Tissue specificityi

Detected in brain (at protein level). Brain (neocortex, hippocampus, amygdala, medial habenula, hypothalamus, locus ceruleus, and parabrachial nucleus).2 Publications

Gene expression databases

ArrayExpressiP33534.
BgeeiP33534.
CleanExiMM_OPRK1.
GenevestigatoriP33534.

Interactioni

Subunit structurei

Interacts with SLC9A3R1. Interacts with GABARAPL1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliP33534.
SMRiP33534. Positions 55-347.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG317066.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP33534.
KOiK04214.
OMAiPFQSTEY.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP33534.
TreeFamiTF315737.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33534-1 [UniParc]FASTAAdd to Basket

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MESPIQIFRG DPGPTCSPSA CLLPNSSSWF PNWAESDSNG SVGSEDQQLE    50
SAHISPAIPV IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN 100
LALADALVTT TMPFQSAVYL MNSWPFGDVL CKIVISIDYY NMFTSIFTLT 150
MMSVDRYIAV CHPVKALDFR TPLKAKIINI CIWLLASSVG ISAIVLGGTK 200
VREDVDVIEC SLQFPDDEYS WWDLFMKICV FVFAFVIPVL IIIVCYTLMI 250
LRLKSVRLLS GSREKDRNLR RITKLVLVVV AVFIICWTPI HIFILVEALG 300
STSHSTAALS SYYFCIALGY TNSSLNPVLY AFLDENFKRC FRDFCFPIKM 350
RMERQSTNRV RNTVQDPASM RDVGGMNKPV 380
Length:380
Mass (Da):42,652
Last modified:February 1, 1994 - v1
Checksum:iFA4FC947D4545318
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti211 – 2111S → L in BAA06508. 1 Publication
Sequence conflicti231 – 2311F → V in BAA06508. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11065 mRNA. Translation: AAA39363.1.
D31665 Genomic DNA. Translation: BAA06508.1.
S77872, S77868, S77869 Genomic DNA. Translation: AAB34130.2.
S81111 mRNA. Translation: AAP32232.1.
CCDSiCCDS14809.1.
PIRiA48227.
JC2434.
RefSeqiNP_001191300.1. NM_001204371.1.
NP_035141.1. NM_011011.2.
UniGeneiMm.7977.

Genome annotation databases

EnsembliENSMUST00000027038; ENSMUSP00000027038; ENSMUSG00000025905.
ENSMUST00000160339; ENSMUSP00000124030; ENSMUSG00000025905.
ENSMUST00000160777; ENSMUSP00000125105; ENSMUSG00000025905.
GeneIDi18387.
KEGGimmu:18387.
UCSCiuc007afo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11065 mRNA. Translation: AAA39363.1 .
D31665 Genomic DNA. Translation: BAA06508.1 .
S77872 , S77868 , S77869 Genomic DNA. Translation: AAB34130.2 .
S81111 mRNA. Translation: AAP32232.1 .
CCDSi CCDS14809.1.
PIRi A48227.
JC2434.
RefSeqi NP_001191300.1. NM_001204371.1.
NP_035141.1. NM_011011.2.
UniGenei Mm.7977.

3D structure databases

ProteinModelPortali P33534.
SMRi P33534. Positions 55-347.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P33534.
ChEMBLi CHEMBL2095192.
GuidetoPHARMACOLOGYi 318.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P33534.

Proteomic databases

PRIDEi P33534.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027038 ; ENSMUSP00000027038 ; ENSMUSG00000025905 .
ENSMUST00000160339 ; ENSMUSP00000124030 ; ENSMUSG00000025905 .
ENSMUST00000160777 ; ENSMUSP00000125105 ; ENSMUSG00000025905 .
GeneIDi 18387.
KEGGi mmu:18387.
UCSCi uc007afo.2. mouse.

Organism-specific databases

CTDi 4986.
MGIi MGI:97439. Oprk1.

Phylogenomic databases

eggNOGi NOG317066.
HOGENOMi HOG000230486.
HOVERGENi HBG106919.
InParanoidi P33534.
KOi K04214.
OMAi PFQSTEY.
OrthoDBi EOG7BKCVQ.
PhylomeDBi P33534.
TreeFami TF315737.

Miscellaneous databases

NextBioi 293980.
PROi P33534.
SOURCEi Search...

Gene expression databases

ArrayExpressi P33534.
Bgeei P33534.
CleanExi MM_OPRK1.
Genevestigatori P33534.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and functional comparison of kappa and delta opioid receptors from mouse brain."
    Yasuda K., Raynor K., Kong H., Breder C.D., Takeda J., Reisine T., Bell G.I.
    Proc. Natl. Acad. Sci. U.S.A. 90:6736-6740(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Structure and chromosomal mapping of genes for the mouse kappa-opioid receptor and an opioid receptor homologue (MOR-C)."
    Nishi M., Takeshima H., Mori M., Nakagawara K., Takeuchi T.
    Biochem. Biophys. Res. Commun. 205:1353-1357(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequence of kappa-opioid receptor cDNA in the R1.1 thymoma cell line."
    Belkowski S.M., Zhu J., Liu-Chen L.Y., Eisenstein T.K., Adler M.W., Rogers T.J.
    J. Neuroimmunol. 62:113-117(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Disruption of the kappa-opioid receptor gene in mice enhances sensitivity to chemical visceral pain, impairs pharmacological actions of the selective kappa-agonist U-50,488H and attenuates morphine withdrawal."
    Simonin F., Valverde O., Smadja C., Slowe S., Kitchen I., Dierich A., Le Meur M., Roques B.P., Maldonado R., Kieffer B.L.
    EMBO J. 17:886-897(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiOPRK_MOUSE
AccessioniPrimary (citable) accession number: P33534
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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