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P33534

- OPRK_MOUSE

UniProt

P33534 - OPRK_MOUSE

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Protein

Kappa-type opioid receptor

Gene

Oprk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

G-protein coupled opioid receptor that functions as receptor for endogenous alpha-neoendorphins and dynorphins, but has low affinity for beta-endorphins. Also functions as receptor for various synthetic opioids and for the psychoactive diterpene salvinorin A. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain. Plays a role in mediating reduced physical activity upon treatment with synthetic opioids. Plays a role in the regulation of salivation in response to synthetic opioids. May play a role in arousal and regulation of autonomic and neuroendocrine functions.2 Publications

GO - Molecular functioni

  1. dynorphin receptor activity Source: UniProtKB
  2. opioid receptor activity Source: UniProtKB

GO - Biological processi

  1. adenylate cyclase-inhibiting opioid receptor signaling pathway Source: UniProtKB
  2. defense response to virus Source: Ensembl
  3. immune response Source: Ensembl
  4. locomotory behavior Source: UniProtKB
  5. phospholipase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
  6. regulation of saliva secretion Source: UniProtKB
  7. sensory perception of pain Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Names & Taxonomyi

Protein namesi
Recommended name:
Kappa-type opioid receptor
Short name:
K-OR-1
Short name:
KOR-1
Alternative name(s):
MSL-1
Gene namesi
Name:Oprk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:97439. Oprk1.

Subcellular locationi

Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB
  2. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate and show no obvious phenotype. Mutant mice do not display analgesia after treatment with the synthetic agonist U-50,488H. Unlike wild-type mice, they do not show reduced locomotion and increased salivation in response to the synthetic agonist U-50,488H.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Kappa-type opioid receptorPRO_0000069968Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi25 – 251N-linked (GlcNAc...)By similarity
Glycosylationi39 – 391N-linked (GlcNAc...)By similarity
Disulfide bondi131 ↔ 210PROSITE-ProRule annotation
Lipidationi345 – 3451S-palmitoyl cysteineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PRIDEiP33534.

PTM databases

PhosphoSiteiP33534.

Expressioni

Tissue specificityi

Detected in brain (at protein level). Brain (neocortex, hippocampus, amygdala, medial habenula, hypothalamus, locus ceruleus, and parabrachial nucleus).2 Publications

Gene expression databases

BgeeiP33534.
CleanExiMM_OPRK1.
ExpressionAtlasiP33534. baseline and differential.
GenevestigatoriP33534.

Interactioni

Subunit structurei

Interacts with SLC9A3R1. Interacts with GABARAPL1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP33534.
SMRiP33534. Positions 55-347.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5757ExtracellularBy similarityAdd
BLAST
Topological domaini86 – 9510CytoplasmicBy similarity
Topological domaini120 – 13213ExtracellularBy similarityAdd
BLAST
Topological domaini155 – 17319CytoplasmicBy similarityAdd
BLAST
Topological domaini197 – 22226ExtracellularBy similarityAdd
BLAST
Topological domaini248 – 27427CytoplasmicBy similarityAdd
BLAST
Topological domaini297 – 31115ExtracellularBy similarityAdd
BLAST
Topological domaini334 – 38047CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei58 – 8528Helical; Name=1By similarityAdd
BLAST
Transmembranei96 – 11924Helical; Name=2By similarityAdd
BLAST
Transmembranei133 – 15422Helical; Name=3By similarityAdd
BLAST
Transmembranei174 – 19623Helical; Name=4By similarityAdd
BLAST
Transmembranei223 – 24725Helical; Name=5By similarityAdd
BLAST
Transmembranei275 – 29622Helical; Name=6By similarityAdd
BLAST
Transmembranei312 – 33322Helical; Name=7By similarityAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG317066.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP33534.
KOiK04214.
OMAiPFQSTEY.
OrthoDBiEOG7BKCVQ.
PhylomeDBiP33534.
TreeFamiTF315737.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33534 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESPIQIFRG DPGPTCSPSA CLLPNSSSWF PNWAESDSNG SVGSEDQQLE
60 70 80 90 100
SAHISPAIPV IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN
110 120 130 140 150
LALADALVTT TMPFQSAVYL MNSWPFGDVL CKIVISIDYY NMFTSIFTLT
160 170 180 190 200
MMSVDRYIAV CHPVKALDFR TPLKAKIINI CIWLLASSVG ISAIVLGGTK
210 220 230 240 250
VREDVDVIEC SLQFPDDEYS WWDLFMKICV FVFAFVIPVL IIIVCYTLMI
260 270 280 290 300
LRLKSVRLLS GSREKDRNLR RITKLVLVVV AVFIICWTPI HIFILVEALG
310 320 330 340 350
STSHSTAALS SYYFCIALGY TNSSLNPVLY AFLDENFKRC FRDFCFPIKM
360 370 380
RMERQSTNRV RNTVQDPASM RDVGGMNKPV
Length:380
Mass (Da):42,652
Last modified:February 1, 1994 - v1
Checksum:iFA4FC947D4545318
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti211 – 2111S → L in BAA06508. (PubMed:7802669)Curated
Sequence conflicti231 – 2311F → V in BAA06508. (PubMed:7802669)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11065 mRNA. Translation: AAA39363.1.
D31665 Genomic DNA. Translation: BAA06508.1.
S77872, S77868, S77869 Genomic DNA. Translation: AAB34130.2.
S81111 mRNA. Translation: AAP32232.1.
CCDSiCCDS14809.1.
PIRiA48227.
JC2434.
RefSeqiNP_001191300.1. NM_001204371.1.
NP_035141.1. NM_011011.2.
UniGeneiMm.7977.

Genome annotation databases

EnsembliENSMUST00000027038; ENSMUSP00000027038; ENSMUSG00000025905.
ENSMUST00000160339; ENSMUSP00000124030; ENSMUSG00000025905.
ENSMUST00000160777; ENSMUSP00000125105; ENSMUSG00000025905.
GeneIDi18387.
KEGGimmu:18387.
UCSCiuc007afo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11065 mRNA. Translation: AAA39363.1 .
D31665 Genomic DNA. Translation: BAA06508.1 .
S77872 , S77868 , S77869 Genomic DNA. Translation: AAB34130.2 .
S81111 mRNA. Translation: AAP32232.1 .
CCDSi CCDS14809.1.
PIRi A48227.
JC2434.
RefSeqi NP_001191300.1. NM_001204371.1.
NP_035141.1. NM_011011.2.
UniGenei Mm.7977.

3D structure databases

ProteinModelPortali P33534.
SMRi P33534. Positions 55-347.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P33534.
ChEMBLi CHEMBL4329.
GuidetoPHARMACOLOGYi 318.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P33534.

Proteomic databases

PRIDEi P33534.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027038 ; ENSMUSP00000027038 ; ENSMUSG00000025905 .
ENSMUST00000160339 ; ENSMUSP00000124030 ; ENSMUSG00000025905 .
ENSMUST00000160777 ; ENSMUSP00000125105 ; ENSMUSG00000025905 .
GeneIDi 18387.
KEGGi mmu:18387.
UCSCi uc007afo.2. mouse.

Organism-specific databases

CTDi 4986.
MGIi MGI:97439. Oprk1.

Phylogenomic databases

eggNOGi NOG317066.
HOGENOMi HOG000230486.
HOVERGENi HBG106919.
InParanoidi P33534.
KOi K04214.
OMAi PFQSTEY.
OrthoDBi EOG7BKCVQ.
PhylomeDBi P33534.
TreeFami TF315737.

Miscellaneous databases

NextBioi 293980.
PROi P33534.
SOURCEi Search...

Gene expression databases

Bgeei P33534.
CleanExi MM_OPRK1.
ExpressionAtlasi P33534. baseline and differential.
Genevestigatori P33534.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and functional comparison of kappa and delta opioid receptors from mouse brain."
    Yasuda K., Raynor K., Kong H., Breder C.D., Takeda J., Reisine T., Bell G.I.
    Proc. Natl. Acad. Sci. U.S.A. 90:6736-6740(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Structure and chromosomal mapping of genes for the mouse kappa-opioid receptor and an opioid receptor homologue (MOR-C)."
    Nishi M., Takeshima H., Mori M., Nakagawara K., Takeuchi T.
    Biochem. Biophys. Res. Commun. 205:1353-1357(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequence of kappa-opioid receptor cDNA in the R1.1 thymoma cell line."
    Belkowski S.M., Zhu J., Liu-Chen L.Y., Eisenstein T.K., Adler M.W., Rogers T.J.
    J. Neuroimmunol. 62:113-117(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Disruption of the kappa-opioid receptor gene in mice enhances sensitivity to chemical visceral pain, impairs pharmacological actions of the selective kappa-agonist U-50,488H and attenuates morphine withdrawal."
    Simonin F., Valverde O., Smadja C., Slowe S., Kitchen I., Dierich A., Le Meur M., Roques B.P., Maldonado R., Kieffer B.L.
    EMBO J. 17:886-897(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiOPRK_MOUSE
AccessioniPrimary (citable) accession number: P33534
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3