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Protein

Vitamin B6 transporter bsu1

Gene

bsu1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Thiamine-regulated, high affinity import carrier of pyridoxine, pyridoxal and pyridoxamine. Also imports, but does not export, amiloride and so confers sensitivity.1 Publication

GO - Molecular functioni

  • pyridoxal transmembrane transporter activity Source: PomBase
  • pyridoxamine transmembrane transporter activity Source: PomBase
  • pyridoxine transmembrane transporter activity Source: PomBase
  • thiamine transmembrane transporter activity Source: PomBase

GO - Biological processi

  • pyridoxal transmembrane transport Source: PomBase
  • pyridoxamine transmembrane transport Source: PomBase
  • pyridoxine transmembrane transport Source: PomBase
  • thiamine transmembrane transport Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Transport

Protein family/group databases

TCDBi2.A.1.2.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B6 transporter bsu1
Alternative name(s):
Amiloride transporter car1
Changed amiloride resistance protein 1
Vitamin B6 uptake protein 1
Gene namesi
Name:bsu1
Synonyms:car1, sod1
ORF Names:SPAC17A2.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC17A2.01.
PomBaseiSPAC17A2.01. bsu1.

Subcellular locationi

  • Membrane 1 Publication; Multi-pass membrane protein 1 Publication

  • Note: During mitosis, localizes also to the septum.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei81 – 10121HelicalSequence analysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence analysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence analysisAdd
BLAST
Transmembranei173 – 19220HelicalSequence analysisAdd
BLAST
Transmembranei204 – 22421HelicalSequence analysisAdd
BLAST
Transmembranei238 – 25720HelicalSequence analysisAdd
BLAST
Transmembranei314 – 33017HelicalSequence analysisAdd
BLAST
Transmembranei349 – 36618HelicalSequence analysisAdd
BLAST
Transmembranei387 – 40721HelicalSequence analysisAdd
BLAST
Transmembranei413 – 43220HelicalSequence analysisAdd
BLAST
Transmembranei444 – 46118HelicalSequence analysisAdd
BLAST
Transmembranei480 – 50122HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: PomBase
  • vacuole Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526Vitamin B6 transporter bsu1PRO_0000173433Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi280536. 2 interactions.
MINTiMINT-4688540.

Structurei

3D structure databases

ProteinModelPortaliP33532.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000095270.
InParanoidiP33532.
OMAiWISERSA.
OrthoDBiEOG7XDBVW.
PhylomeDBiP33532.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKIASLFS PSETASKDQH ENVAEDLELG TASSQSDGIH ETNSEYDEKK
60 70 80 90 100
REESPEVIDI SNLISSDHPA HPQNWHWAKR WSIVFMFCLM QIYVIWTSNG
110 120 130 140 150
FGSIEYSVMA QFNVSAQVAT LCLSMNILGS GLGPMFLGPL SDIGGRKPVY
160 170 180 190 200
FCSIFVYTVF NISCALPRNI VQMIISHFII GVAGSTALTN VAGGIPDLFP
210 220 230 240 250
EDTAGVPMSL FVWACAGGAI GAPMATGVDI NAKYGWRWLY YINIIVGGFF
260 270 280 290 300
LIVILIIPET LPIKVITRYE NAKGRIVEGI PKNNLKEVLK KCKFVTTMGF
310 320 330 340 350
RMMLTEPIIL SMGLYNFYAY GISYFFLTAI WPVFYDTYKM SEMGASCTYL
360 370 380 390 400
SGFVASTLLF LYQPIQDWIF RRDKAKNNGV ARPEARFTSA LFITLLFPAG
410 420 430 440 450
MFLFAFTCHP PFPWMSPIVG NSMVTVANGH NWMCILNYLT DSYPLLSGSA
460 470 480 490 500
VAAFTLPSFI GATVFAHVSQ IMFNNMSVKW AVATMAFISI SIPFIIYTFY
510 520
FFGQRIRALS SLTGNKALKY LPLENN
Length:526
Mass (Da):58,484
Last modified:July 15, 1998 - v2
Checksum:iFD5725A338BF454E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 375SSQSD → PLNQI in CAA78411 (PubMed:8246883).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14035 Genomic DNA. Translation: CAA78411.1.
CU329670 Genomic DNA. Translation: CAB11084.1.
PIRiS39919.
RefSeqiXP_001713084.1. XM_001713032.2.

Genome annotation databases

EnsemblFungiiSPAC17A2.01.1; SPAC17A2.01.1:pep; SPAC17A2.01.
GeneIDi3361460.
KEGGispo:SPAC17A2.01.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14035 Genomic DNA. Translation: CAA78411.1.
CU329670 Genomic DNA. Translation: CAB11084.1.
PIRiS39919.
RefSeqiXP_001713084.1. XM_001713032.2.

3D structure databases

ProteinModelPortaliP33532.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280536. 2 interactions.
MINTiMINT-4688540.

Protein family/group databases

TCDBi2.A.1.2.1. the major facilitator superfamily (mfs).

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC17A2.01.1; SPAC17A2.01.1:pep; SPAC17A2.01.
GeneIDi3361460.
KEGGispo:SPAC17A2.01.

Organism-specific databases

EuPathDBiFungiDB:SPAC17A2.01.
PomBaseiSPAC17A2.01. bsu1.

Phylogenomic databases

HOGENOMiHOG000095270.
InParanoidiP33532.
OMAiWISERSA.
OrthoDBiEOG7XDBVW.
PhylomeDBiP33532.

Miscellaneous databases

NextBioi20811504.
PROiP33532.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The amiloride resistance gene, car1, of Schizosaccharomyces pombe."
    Jia Z.-P., McCullough N., Wong L., Young P.G.
    Mol. Gen. Genet. 241:298-304(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Amiloride uptake and toxicity in fission yeast are caused by the pyridoxine transporter encoded by bsu1+ (car1+)."
    Stolz J., Woehrmann H.J., Vogl C.
    Eukaryot. Cell 4:319-326(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiBSU1_SCHPO
AccessioniPrimary (citable) accession number: P33532
Secondary accession number(s): O13866
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 15, 1998
Last modified: February 17, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.