Reviewed, UniProtKB/Swiss-Prot P33527 (MRP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Multidrug resistance-associated protein 1
Alternative name(s):
    ATP-binding cassette sub-family C member 1
    Leukotriene C(4) transporter
      Short name=LTC4 transporter

Gene names

Name:	ABCC1
Synonyms:	MRP, MRP1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1531 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency. Ref.11 Ref.12 Ref.18
Subcellular location	Cell membrane; Multi-pass membrane protein. Ref.18
Tissue specificity	Lung, testis and peripheral blood mononuclear cells.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	response to drug Ref.1 Traceable author statement. Source: ProtInc transport Ref.1 Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc membrane fraction Ref.1 Traceable author statement. Source: ProtInc
Molecular function	ATP binding Ref.1 Traceable author statement. Source: ProtInc ATPase activity, coupled to transmembrane movement of substances Ref.1 Inferred from Experiment. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 9 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: P33527-1)

Also known as: Allexons;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P33527-2)

Also known as: Delexon-17;

The sequence of this isoform differs from the canonical sequence as follows:
     706-764: Missing.

Isoform 3 (identifier: P33527-3)

Also known as: Delexon-18;

The sequence of this isoform differs from the canonical sequence as follows:
     765-820: Missing.

Isoform 4 (identifier: P33527-4)

Also known as: Delexon-30;

The sequence of this isoform differs from the canonical sequence as follows:
     1431-1495: Missing.

Isoform 5 (identifier: P33527-5)

Also known as: Delexon-17-18;

The sequence of this isoform differs from the canonical sequence as follows:
     706-764: Missing.
     765-820: Missing.

Isoform 6 (identifier: P33527-6)

Also known as: Delexon-17-30;

The sequence of this isoform differs from the canonical sequence as follows:
     706-764: Missing.
     1431-1495: Missing.

Isoform 7 (identifier: P33527-7)

Also known as: Delexon-18-30;

The sequence of this isoform differs from the canonical sequence as follows:
     765-820: Missing.
     1431-1495: Missing.

Isoform 8 (identifier: P33527-8)

Also known as: Delexon-17-18-30;

The sequence of this isoform differs from the canonical sequence as follows:
     706-764: Missing.
     765-820: Missing.
     1431-1495: Missing.

Isoform 9 (identifier: P33527-9)

The sequence of this isoform differs from the canonical sequence as follows:
     882-882: G → GSTVMDEEEAG

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1531

1531

Multidrug resistance-associated protein 1

PRO_0000093351

Regions

Topological domain

1 – 33

33

Extracellular Ref.8 Ref.9 Ref.10

Transmembrane

34 – 54

21

1

Topological domain

55 – 74

20

Cytoplasmic Ref.8 Ref.9 Ref.10

Transmembrane

75 – 95

21

2

Topological domain

96 – 100

5

Extracellular Ref.8 Ref.9 Ref.10

Transmembrane

101 – 121

21

3

Topological domain

122 – 133

12

Cytoplasmic Ref.8 Ref.9 Ref.10

Transmembrane

134 – 154

21

4

Topological domain

155 – 172

18

Extracellular Ref.8 Ref.9 Ref.10

Transmembrane

173 – 193

21

5

Topological domain

194 – 316

123

Cytoplasmic Ref.8 Ref.9 Ref.10

Transmembrane

317 – 337

21

6

Topological domain

338 – 363

26

Extracellular Ref.8 Ref.9 Ref.10

Transmembrane

364 – 384

21

7

Topological domain

385 – 440

56

Cytoplasmic Ref.8 Ref.9 Ref.10

Transmembrane

441 – 461

21

8

Topological domain

462 – 464

3

Extracellular Ref.8 Ref.9 Ref.10

Transmembrane

465 – 485

21

9

Topological domain

486 – 547

62

Cytoplasmic Ref.8 Ref.9 Ref.10

Transmembrane

548 – 568

21

10

Topological domain

569 – 590

22

Extracellular Ref.8 Ref.9 Ref.10

Transmembrane

591 – 611

21

11

Topological domain

612 – 967

356

Cytoplasmic Ref.8 Ref.9 Ref.10

Transmembrane

968 – 988

21

12

Topological domain

989 – 1025

37

Extracellular Ref.8 Ref.9 Ref.10

Transmembrane

1026 – 1046

21

13

Topological domain

1047 – 1089

43

Cytoplasmic Ref.8 Ref.9 Ref.10

Transmembrane

1090 – 1110

21

14

Topological domain

1111

1

Extracellular Ref.8 Ref.9 Ref.10

Transmembrane

1112 – 1132

21

15

Topological domain

1133 – 1203

71

Cytoplasmic Ref.8 Ref.9 Ref.10

Transmembrane

1204 – 1224

21

16

Topological domain

1225 – 1226

2

Extracellular Ref.8 Ref.9 Ref.10

Transmembrane

1227 – 1247

21

17

Topological domain

1248 – 1531

284

Cytoplasmic Ref.8 Ref.9 Ref.10

Domain

325 – 608

284

ABC transmembrane type-1 1

Domain

644 – 868

225

ABC transporter 1

Domain

975 – 1256

282

ABC transmembrane type-1 2

Domain

1293 – 1527

235

ABC transporter 2

Nucleotide binding

678 – 685

8

ATP 1

Nucleotide binding

1327 – 1334

8

ATP 2 Potential

Sites

Binding site

653

1

ATP 1

Binding site

713

1

ATP 1

Amino acid modifications

Modified residue

915

1

Phosphoserine Ref.19

Glycosylation

19

1

N-linked (GlcNAc...) Ref.8

Glycosylation

23

1

N-linked (GlcNAc...) Ref.8

Glycosylation

1006

1

N-linked (GlcNAc...) Ref.8

Natural variations

Alternative sequence

706 – 764

59

Missing in isoform 2, isoform 5, isoform 6 and isoform 8.

VSP_000037

Alternative sequence

765 – 820

56

Missing in isoform 3, isoform 5, isoform 7 and isoform 8.

VSP_000038

Alternative sequence

882

1

G → GSTVMDEEEAG in isoform 9.

VSP_017014

Alternative sequence

1431 – 1495

65

Missing in isoform 4, isoform 6, isoform 7 and isoform 8.

VSP_000039

Natural variant

43

1

C → S

VAR_013317

Natural variant

73

1

T → I

VAR_013318

Natural variant

117

1

T → M

VAR_013319

Natural variant

433

1

R → S

VAR_013320

Natural variant

633

1

R → Q

VAR_011488

Natural variant

671

1

G → V No effect on leukotriene C4 and estradiol glucuronide transport. dbSNP rs45511401. Ref.4 Ref.21 Ref.22 Ref.23

VAR_011489

Natural variant

723

1

R → Q: dbSNP rs4148356. Ref.4 Ref.25

VAR_013321

Natural variant

861

1

A → T: dbSNP rs45517537. Ref.4

VAR_055384

Natural variant

1047

1

C → S: dbSNP rs13337489. Ref.4

VAR_055385

Natural variant

1058

1

R → Q

VAR_013322

Natural variant

1146

1

V → I: dbSNP rs28706727. Ref.4

VAR_055386

Natural variant

1512

1

S → L

VAR_013323

Experimental info

Mutagenesis

580

1

Q → A: No effect. Ref.17

Mutagenesis

581

1

T → A: No effect. Ref.17

Mutagenesis

585

1

S → A: No effect. Ref.17

Mutagenesis

597

1

N → A: Increases resistance to vincristine and decreases resistance to VP-16. Ref.17

Mutagenesis

604

1

S → A: Increases estradiol glucuronide transport. Ref.17

Mutagenesis

605

1

S → A: Decreases resistance to vincristine, VP-16 and doxorubicin. Ref.17

Mutagenesis

792

1

D → A: Only partially affects protein maturation; impairs leukotriene C4 transport. Ref.13

Mutagenesis

792

1

D → L: Impairs protein maturation and leukotriene C4 transport. Ref.13

Mutagenesis

793

1

D → L: No effect on protein maturation and leukotriene C4 transport. Ref.13

Mutagenesis

1046

1

R → D: Slightly impairs leukotriene C4 and estradiol glucuronide transport. Ref.16

Mutagenesis

1084

1

D → R: Impairs leukotriene C4 and estradiol glucuronide transport. Ref.16

Mutagenesis

1089

1

E → A, L, N or Q: Decreases resistance to anthracyclines. Ref.15

Mutagenesis

1089

1

E → D: No effect. Ref.15

Mutagenesis

1089

1

E → K: Abolishes resistance to anthracyclines. Ref.15

Mutagenesis

1131

1

R → E: Slightly impairs leukotriene C4 and estradiol glucuronide transport. Ref.16

Mutagenesis

1138

1

R → E or K: Strongly reduced transport of leukotriene C4, estradiol glucuronide and of glutathione. Ref.18

Mutagenesis

1141

1

K → E: Reduced transport of leukotriene C4 and of glutathione. Ref.18

Mutagenesis

1141

1

K → R: Reduced transport of glutathione. Ref.18

Mutagenesis

1142

1

R → E or K: Reduced transport of leukotriene C4, estradiol glucuronide and of glutathione. Ref.18

Mutagenesis

1246

1

W → A, F or Y: Impairs estradiol glucuronide transport. Ref.14

Mutagenesis

1246

1

W → C: Impairs estradiol glucuronide transport; loss of resistance to alkaloid vincristine, cationic anthracyclines, epipodophyllotoxin VP-16, but not potassium antimony tartrate; partial loss of resistance to sodium arsenite. Ref.14

Mutagenesis

1333

1

K → L: Impairs leukotriene C4 transport. Ref.13

Mutagenesis

1454 – 1455

2

DE → LL: Impairs leukotriene C4 transport.

Secondary structure

1

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1531

Helix Strand Turn

Details...

Beta strand

644 – 653

10

Beta strand

660 – 668

9

Beta strand

673 – 677

5

Helix

684 – 691

8

Beta strand

695 – 704

10

Beta strand

708 – 711

4

Beta strand

719 – 721

3

Helix

722 – 727

6

Helix

736 – 743

8

Helix

747 – 750

4

Helix

756 – 758

3

Beta strand

759 – 762

4

Helix

770 – 784

15

Beta strand

787 – 793

7

Turn

794 – 797

4

Helix

800 – 809

10

Turn

816 – 819

4

Beta strand

820 – 825

6

Helix

832 – 834

3

Beta strand

835 – 842

8

Beta strand

845 – 850

6

Helix

852 – 858

7

Helix

861 – 868

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Allexons) [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 5CCB92FB7B096B52	FASTA	1,531	171,561
	10 20 30 40 50 60 MALRGFCSAD GSDPLWDWNV TWNTSNPDFT KCFQNTVLVW VPCFYLWACF PFYFLYLSRH 70 80 90 100 110 120 DRGYIQMTPL NKTKTALGFL LWIVCWADLF YSFWERSRGI FLAPVFLVSP TLLGITTLLA 130 140 150 160 170 180 TFLIQLERRK GVQSSGIMLT FWLVALVCAL AILRSKIMTA LKEDAQVDLF RDITFYVYFS 190 200 210 220 230 240 LLLIQLVLSC FSDRSPLFSE TIHDPNPCPE SSASFLSRIT FWWITGLIVR GYRQPLEGSD 250 260 270 280 290 300 LWSLNKEDTS EQVVPVLVKN WKKECAKTRK QPVKVVYSSK DPAQPKESSK VDANEEVEAL 310 320 330 340 350 360 IVKSPQKEWN PSLFKVLYKT FGPYFLMSFF FKAIHDLMMF SGPQILKLLI KFVNDTKAPD 370 380 390 400 410 420 WQGYFYTVLL FVTACLQTLV LHQYFHICFV SGMRIKTAVI GAVYRKALVI TNSARKSSTV 430 440 450 460 470 480 GEIVNLMSVD AQRFMDLATY INMIWSAPLQ VILALYLLWL NLGPSVLAGV AVMVLMVPVN 490 500 510 520 530 540 AVMAMKTKTY QVAHMKSKDN RIKLMNEILN GIKVLKLYAW ELAFKDKVLA IRQEELKVLK 550 560 570 580 590 600 KSAYLSAVGT FTWVCTPFLV ALCTFAVYVT IDENNILDAQ TAFVSLALFN ILRFPLNILP 610 620 630 640 650 660 MVISSIVQAS VSLKRLRIFL SHEELEPDSI ERRPVKDGGG TNSITVRNAT FTWARSDPPT 670 680 690 700 710 720 LNGITFSIPE GALVAVVGQV GCGKSSLLSA LLAEMDKVEG HVAIKGSVAY VPQQAWIQND 730 740 750 760 770 780 SLRENILFGC QLEEPYYRSV IQACALLPDL EILPSGDRTE IGEKGVNLSG GQKQRVSLAR 790 800 810 820 830 840 AVYSNADIYL FDDPLSAVDA HVGKHIFENV IGPKGMLKNK TRILVTHSMS YLPQVDVIIV 850 860 870 880 890 900 MSGGKISEMG SYQELLARDG AFAEFLRTYA STEQEQDAEE NGVTGVSGPG KEAKQMENGM 910 920 930 940 950 960 LVTDSAGKQL QRQLSSSSSY SGDISRHHNS TAELQKAEAK KEETWKLMEA DKAQTGQVKL 970 980 990 1000 1010 1020 SVYWDYMKAI GLFISFLSIF LFMCNHVSAL ASNYWLSLWT DDPIVNGTQE HTKVRLSVYG 1030 1040 1050 1060 1070 1080 ALGISQGIAV FGYSMAVSIG GILASRCLHV DLLHSILRSP MSFFERTPSG NLVNRFSKEL 1090 1100 1110 1120 1130 1140 DTVDSMIPEV IKMFMGSLFN VIGACIVILL ATPIAAIIIP PLGLIYFFVQ RFYVASSRQL 1150 1160 1170 1180 1190 1200 KRLESVSRSP VYSHFNETLL GVSVIRAFEE QERFIHQSDL KVDENQKAYY PSIVANRWLA 1210 1220 1230 1240 1250 1260 VRLECVGNCI VLFAALFAVI SRHSLSAGLV GLSVSYSLQV TTYLNWLVRM SSEMETNIVA 1270 1280 1290 1300 1310 1320 VERLKEYSET EKEAPWQIQE TAPPSSWPQV GRVEFRNYCL RYREDLDFVL RHINVTINGG 1330 1340 1350 1360 1370 1380 EKVGIVGRTG AGKSSLTLGL FRINESAEGE IIIDGINIAK IGLHDLRFKI TIIPQDPVLF 1390 1400 1410 1420 1430 1440 SGSLRMNLDP FSQYSDEEVW TSLELAHLKD FVSALPDKLD HECAEGGENL SVGQRQLVCL 1450 1460 1470 1480 1490 1500 ARALLRKTKI LVLDEATAAV DLETDDLIQS TIRTQFEDCT VLTIAHRLNT IMDYTRVIVL 1510 1520 1530 DKGEIQEYGA PSDLLQQRGL FYSMAKDAGL V
	Isoform 2 (Delexon-17). Checksum: 135C0953D5EDA403	FASTA	1,472	164,941
	10 20 30 40 50 60 MALRGFCSAD GSDPLWDWNV TWNTSNPDFT KCFQNTVLVW VPCFYLWACF PFYFLYLSRH 70 80 90 100 110 120 DRGYIQMTPL NKTKTALGFL LWIVCWADLF YSFWERSRGI FLAPVFLVSP TLLGITTLLA 130 140 150 160 170 180 TFLIQLERRK GVQSSGIMLT FWLVALVCAL AILRSKIMTA LKEDAQVDLF RDITFYVYFS 190 200 210 220 230 240 LLLIQLVLSC FSDRSPLFSE TIHDPNPCPE SSASFLSRIT FWWITGLIVR GYRQPLEGSD 250 260 270 280 290 300 LWSLNKEDTS EQVVPVLVKN WKKECAKTRK QPVKVVYSSK DPAQPKESSK VDANEEVEAL 310 320 330 340 350 360 IVKSPQKEWN PSLFKVLYKT FGPYFLMSFF FKAIHDLMMF SGPQILKLLI KFVNDTKAPD 370 380 390 400 410 420 WQGYFYTVLL FVTACLQTLV LHQYFHICFV SGMRIKTAVI GAVYRKALVI TNSARKSSTV 430 440 450 460 470 480 GEIVNLMSVD AQRFMDLATY INMIWSAPLQ VILALYLLWL NLGPSVLAGV AVMVLMVPVN 490 500 510 520 530 540 AVMAMKTKTY QVAHMKSKDN RIKLMNEILN GIKVLKLYAW ELAFKDKVLA IRQEELKVLK 550 560 570 580 590 600 KSAYLSAVGT FTWVCTPFLV ALCTFAVYVT IDENNILDAQ TAFVSLALFN ILRFPLNILP 610 620 630 640 650 660 MVISSIVQAS VSLKRLRIFL SHEELEPDSI ERRPVKDGGG TNSITVRNAT FTWARSDPPT 670 680 690 700 710 720 LNGITFSIPE GALVAVVGQV GCGKSSLLSA LLAEMDKVEG HVAIKGVNLS GGQKQRVSLA 730 740 750 760 770 780 RAVYSNADIY LFDDPLSAVD AHVGKHIFEN VIGPKGMLKN KTRILVTHSM SYLPQVDVII 790 800 810 820 830 840 VMSGGKISEM GSYQELLARD GAFAEFLRTY ASTEQEQDAE ENGVTGVSGP GKEAKQMENG 850 860 870 880 890 900 MLVTDSAGKQ LQRQLSSSSS YSGDISRHHN STAELQKAEA KKEETWKLME ADKAQTGQVK 910 920 930 940 950 960 LSVYWDYMKA IGLFISFLSI FLFMCNHVSA LASNYWLSLW TDDPIVNGTQ EHTKVRLSVY 970 980 990 1000 1010 1020 GALGISQGIA VFGYSMAVSI GGILASRCLH VDLLHSILRS PMSFFERTPS GNLVNRFSKE 1030 1040 1050 1060 1070 1080 LDTVDSMIPE VIKMFMGSLF NVIGACIVIL LATPIAAIII PPLGLIYFFV QRFYVASSRQ 1090 1100 1110 1120 1130 1140 LKRLESVSRS PVYSHFNETL LGVSVIRAFE EQERFIHQSD LKVDENQKAY YPSIVANRWL 1150 1160 1170 1180 1190 1200 AVRLECVGNC IVLFAALFAV ISRHSLSAGL VGLSVSYSLQ VTTYLNWLVR MSSEMETNIV 1210 1220 1230 1240 1250 1260 AVERLKEYSE TEKEAPWQIQ ETAPPSSWPQ VGRVEFRNYC LRYREDLDFV LRHINVTING 1270 1280 1290 1300 1310 1320 GEKVGIVGRT GAGKSSLTLG LFRINESAEG EIIIDGINIA KIGLHDLRFK ITIIPQDPVL 1330 1340 1350 1360 1370 1380 FSGSLRMNLD PFSQYSDEEV WTSLELAHLK DFVSALPDKL DHECAEGGEN LSVGQRQLVC 1390 1400 1410 1420 1430 1440 LARALLRKTK ILVLDEATAA VDLETDDLIQ STIRTQFEDC TVLTIAHRLN TIMDYTRVIV 1450 1460 1470 LDKGEIQEYG APSDLLQQRG LFYSMAKDAG LV
	Isoform 3 (Delexon-18). Checksum: 50008274DAD948A2	FASTA	1,475	165,539
	10 20 30 40 50 60 MALRGFCSAD GSDPLWDWNV TWNTSNPDFT KCFQNTVLVW VPCFYLWACF PFYFLYLSRH 70 80 90 100 110 120 DRGYIQMTPL NKTKTALGFL LWIVCWADLF YSFWERSRGI FLAPVFLVSP TLLGITTLLA 130 140 150 160 170 180 TFLIQLERRK GVQSSGIMLT FWLVALVCAL AILRSKIMTA LKEDAQVDLF RDITFYVYFS 190 200 210 220 230 240 LLLIQLVLSC FSDRSPLFSE TIHDPNPCPE SSASFLSRIT FWWITGLIVR GYRQPLEGSD 250 260 270 280 290 300 LWSLNKEDTS EQVVPVLVKN WKKECAKTRK QPVKVVYSSK DPAQPKESSK VDANEEVEAL 310 320 330 340 350 360 IVKSPQKEWN PSLFKVLYKT FGPYFLMSFF FKAIHDLMMF SGPQILKLLI KFVNDTKAPD 370 380 390 400 410 420 WQGYFYTVLL FVTACLQTLV LHQYFHICFV SGMRIKTAVI GAVYRKALVI TNSARKSSTV 430 440 450 460 470 480 GEIVNLMSVD AQRFMDLATY INMIWSAPLQ VILALYLLWL NLGPSVLAGV AVMVLMVPVN 490 500 510 520 530 540 AVMAMKTKTY QVAHMKSKDN RIKLMNEILN GIKVLKLYAW ELAFKDKVLA IRQEELKVLK 550 560 570 580 590 600 KSAYLSAVGT FTWVCTPFLV ALCTFAVYVT IDENNILDAQ TAFVSLALFN ILRFPLNILP 610 620 630 640 650 660 MVISSIVQAS VSLKRLRIFL SHEELEPDSI ERRPVKDGGG TNSITVRNAT FTWARSDPPT 670 680 690 700 710 720 LNGITFSIPE GALVAVVGQV GCGKSSLLSA LLAEMDKVEG HVAIKGSVAY VPQQAWIQND 730 740 750 760 770 780 SLRENILFGC QLEEPYYRSV IQACALLPDL EILPSGDRTE IGEKTRILVT HSMSYLPQVD 790 800 810 820 830 840 VIIVMSGGKI SEMGSYQELL ARDGAFAEFL RTYASTEQEQ DAEENGVTGV SGPGKEAKQM 850 860 870 880 890 900 ENGMLVTDSA GKQLQRQLSS SSSYSGDISR HHNSTAELQK AEAKKEETWK LMEADKAQTG 910 920 930 940 950 960 QVKLSVYWDY MKAIGLFISF LSIFLFMCNH VSALASNYWL SLWTDDPIVN GTQEHTKVRL 970 980 990 1000 1010 1020 SVYGALGISQ GIAVFGYSMA VSIGGILASR CLHVDLLHSI LRSPMSFFER TPSGNLVNRF 1030 1040 1050 1060 1070 1080 SKELDTVDSM IPEVIKMFMG SLFNVIGACI VILLATPIAA IIIPPLGLIY FFVQRFYVAS 1090 1100 1110 1120 1130 1140 SRQLKRLESV SRSPVYSHFN ETLLGVSVIR AFEEQERFIH QSDLKVDENQ KAYYPSIVAN 1150 1160 1170 1180 1190 1200 RWLAVRLECV GNCIVLFAAL FAVISRHSLS AGLVGLSVSY SLQVTTYLNW LVRMSSEMET 1210 1220 1230 1240 1250 1260 NIVAVERLKE YSETEKEAPW QIQETAPPSS WPQVGRVEFR NYCLRYREDL DFVLRHINVT 1270 1280 1290 1300 1310 1320 INGGEKVGIV GRTGAGKSSL TLGLFRINES AEGEIIIDGI NIAKIGLHDL RFKITIIPQD 1330 1340 1350 1360 1370 1380 PVLFSGSLRM NLDPFSQYSD EEVWTSLELA HLKDFVSALP DKLDHECAEG GENLSVGQRQ 1390 1400 1410 1420 1430 1440 LVCLARALLR KTKILVLDEA TAAVDLETDD LIQSTIRTQF EDCTVLTIAH RLNTIMDYTR 1450 1460 1470 VIVLDKGEIQ EYGAPSDLLQ QRGLFYSMAK DAGLV
	Isoform 4 (Delexon-30). Checksum: 36A656CACC393063	FASTA	1,466	164,274
	10 20 30 40 50 60 MALRGFCSAD GSDPLWDWNV TWNTSNPDFT KCFQNTVLVW VPCFYLWACF PFYFLYLSRH 70 80 90 100 110 120 DRGYIQMTPL NKTKTALGFL LWIVCWADLF YSFWERSRGI FLAPVFLVSP TLLGITTLLA 130 140 150 160 170 180 TFLIQLERRK GVQSSGIMLT FWLVALVCAL AILRSKIMTA LKEDAQVDLF RDITFYVYFS 190 200 210 220 230 240 LLLIQLVLSC FSDRSPLFSE TIHDPNPCPE SSASFLSRIT FWWITGLIVR GYRQPLEGSD 250 260 270 280 290 300 LWSLNKEDTS EQVVPVLVKN WKKECAKTRK QPVKVVYSSK DPAQPKESSK VDANEEVEAL 310 320 330 340 350 360 IVKSPQKEWN PSLFKVLYKT FGPYFLMSFF FKAIHDLMMF SGPQILKLLI KFVNDTKAPD 370 380 390 400 410 420 WQGYFYTVLL FVTACLQTLV LHQYFHICFV SGMRIKTAVI GAVYRKALVI TNSARKSSTV 430 440 450 460 470 480 GEIVNLMSVD AQRFMDLATY INMIWSAPLQ VILALYLLWL NLGPSVLAGV AVMVLMVPVN 490 500 510 520 530 540 AVMAMKTKTY QVAHMKSKDN RIKLMNEILN GIKVLKLYAW ELAFKDKVLA IRQEELKVLK 550 560 570 580 590 600 KSAYLSAVGT FTWVCTPFLV ALCTFAVYVT IDENNILDAQ TAFVSLALFN ILRFPLNILP 610 620 630 640 650 660 MVISSIVQAS VSLKRLRIFL SHEELEPDSI ERRPVKDGGG TNSITVRNAT FTWARSDPPT 670 680 690 700 710 720 LNGITFSIPE GALVAVVGQV GCGKSSLLSA LLAEMDKVEG HVAIKGSVAY VPQQAWIQND 730 740 750 760 770 780 SLRENILFGC QLEEPYYRSV IQACALLPDL EILPSGDRTE IGEKGVNLSG GQKQRVSLAR 790 800 810 820 830 840 AVYSNADIYL FDDPLSAVDA HVGKHIFENV IGPKGMLKNK TRILVTHSMS YLPQVDVIIV 850 860 870 880 890 900 MSGGKISEMG SYQELLARDG AFAEFLRTYA STEQEQDAEE NGVTGVSGPG KEAKQMENGM 910 920 930 940 950 960 LVTDSAGKQL QRQLSSSSSY SGDISRHHNS TAELQKAEAK KEETWKLMEA DKAQTGQVKL 970 980 990 1000 1010 1020 SVYWDYMKAI GLFISFLSIF LFMCNHVSAL ASNYWLSLWT DDPIVNGTQE HTKVRLSVYG 1030 1040 1050 1060 1070 1080 ALGISQGIAV FGYSMAVSIG GILASRCLHV DLLHSILRSP MSFFERTPSG NLVNRFSKEL 1090 1100 1110 1120 1130 1140 DTVDSMIPEV IKMFMGSLFN VIGACIVILL ATPIAAIIIP PLGLIYFFVQ RFYVASSRQL 1150 1160 1170 1180 1190 1200 KRLESVSRSP VYSHFNETLL GVSVIRAFEE QERFIHQSDL KVDENQKAYY PSIVANRWLA 1210 1220 1230 1240 1250 1260 VRLECVGNCI VLFAALFAVI SRHSLSAGLV GLSVSYSLQV TTYLNWLVRM SSEMETNIVA 1270 1280 1290 1300 1310 1320 VERLKEYSET EKEAPWQIQE TAPPSSWPQV GRVEFRNYCL RYREDLDFVL RHINVTINGG 1330 1340 1350 1360 1370 1380 EKVGIVGRTG AGKSSLTLGL FRINESAEGE IIIDGINIAK IGLHDLRFKI TIIPQDPVLF 1390 1400 1410 1420 1430 1440 SGSLRMNLDP FSQYSDEEVW TSLELAHLKD FVSALPDKLD HECAEGGENL RVIVLDKGEI 1450 1460 QEYGAPSDLL QQRGLFYSMA KDAGLV
	Isoform 5 (Delexon-17-18). Checksum: A9595FBC7457AEC4	FASTA	1,416	158,919
	10 20 30 40 50 60 MALRGFCSAD GSDPLWDWNV TWNTSNPDFT KCFQNTVLVW VPCFYLWACF PFYFLYLSRH 70 80 90 100 110 120 DRGYIQMTPL NKTKTALGFL LWIVCWADLF YSFWERSRGI FLAPVFLVSP TLLGITTLLA 130 140 150 160 170 180 TFLIQLERRK GVQSSGIMLT FWLVALVCAL AILRSKIMTA LKEDAQVDLF RDITFYVYFS 190 200 210 220 230 240 LLLIQLVLSC FSDRSPLFSE TIHDPNPCPE SSASFLSRIT FWWITGLIVR GYRQPLEGSD 250 260 270 280 290 300 LWSLNKEDTS EQVVPVLVKN WKKECAKTRK QPVKVVYSSK DPAQPKESSK VDANEEVEAL 310 320 330 340 350 360 IVKSPQKEWN PSLFKVLYKT FGPYFLMSFF FKAIHDLMMF SGPQILKLLI KFVNDTKAPD 370 380 390 400 410 420 WQGYFYTVLL FVTACLQTLV LHQYFHICFV SGMRIKTAVI GAVYRKALVI TNSARKSSTV 430 440 450 460 470 480 GEIVNLMSVD AQRFMDLATY INMIWSAPLQ VILALYLLWL NLGPSVLAGV AVMVLMVPVN 490 500 510 520 530 540 AVMAMKTKTY QVAHMKSKDN RIKLMNEILN GIKVLKLYAW ELAFKDKVLA IRQEELKVLK 550 560 570 580 590 600 KSAYLSAVGT FTWVCTPFLV ALCTFAVYVT IDENNILDAQ TAFVSLALFN ILRFPLNILP 610 620 630 640 650 660 MVISSIVQAS VSLKRLRIFL SHEELEPDSI ERRPVKDGGG TNSITVRNAT FTWARSDPPT 670 680 690 700 710 720 LNGITFSIPE GALVAVVGQV GCGKSSLLSA LLAEMDKVEG HVAIKTRILV THSMSYLPQV 730 740 750 760 770 780 DVIIVMSGGK ISEMGSYQEL LARDGAFAEF LRTYASTEQE QDAEENGVTG VSGPGKEAKQ 790 800 810 820 830 840 MENGMLVTDS AGKQLQRQLS SSSSYSGDIS RHHNSTAELQ KAEAKKEETW KLMEADKAQT 850 860 870 880 890 900 GQVKLSVYWD YMKAIGLFIS FLSIFLFMCN HVSALASNYW LSLWTDDPIV NGTQEHTKVR 910 920 930 940 950 960 LSVYGALGIS QGIAVFGYSM AVSIGGILAS RCLHVDLLHS ILRSPMSFFE RTPSGNLVNR 970 980 990 1000 1010 1020 FSKELDTVDS MIPEVIKMFM GSLFNVIGAC IVILLATPIA AIIIPPLGLI YFFVQRFYVA 1030 1040 1050 1060 1070 1080 SSRQLKRLES VSRSPVYSHF NETLLGVSVI RAFEEQERFI HQSDLKVDEN QKAYYPSIVA 1090 1100 1110 1120 1130 1140 NRWLAVRLEC VGNCIVLFAA LFAVISRHSL SAGLVGLSVS YSLQVTTYLN WLVRMSSEME 1150 1160 1170 1180 1190 1200 TNIVAVERLK EYSETEKEAP WQIQETAPPS SWPQVGRVEF RNYCLRYRED LDFVLRHINV 1210 1220 1230 1240 1250 1260 TINGGEKVGI VGRTGAGKSS LTLGLFRINE SAEGEIIIDG INIAKIGLHD LRFKITIIPQ 1270 1280 1290 1300 1310 1320 DPVLFSGSLR MNLDPFSQYS DEEVWTSLEL AHLKDFVSAL PDKLDHECAE GGENLSVGQR 1330 1340 1350 1360 1370 1380 QLVCLARALL RKTKILVLDE ATAAVDLETD DLIQSTIRTQ FEDCTVLTIA HRLNTIMDYT 1390 1400 1410 RVIVLDKGEI QEYGAPSDLL QQRGLFYSMA KDAGLV
	Isoform 6 (Delexon-17-30). Checksum: 40FB7CB148050CDA	FASTA	1,407	157,653
	10 20 30 40 50 60 MALRGFCSAD GSDPLWDWNV TWNTSNPDFT KCFQNTVLVW VPCFYLWACF PFYFLYLSRH 70 80 90 100 110 120 DRGYIQMTPL NKTKTALGFL LWIVCWADLF YSFWERSRGI FLAPVFLVSP TLLGITTLLA 130 140 150 160 170 180 TFLIQLERRK GVQSSGIMLT FWLVALVCAL AILRSKIMTA LKEDAQVDLF RDITFYVYFS 190 200 210 220 230 240 LLLIQLVLSC FSDRSPLFSE TIHDPNPCPE SSASFLSRIT FWWITGLIVR GYRQPLEGSD 250 260 270 280 290 300 LWSLNKEDTS EQVVPVLVKN WKKECAKTRK QPVKVVYSSK DPAQPKESSK VDANEEVEAL 310 320 330 340 350 360 IVKSPQKEWN PSLFKVLYKT FGPYFLMSFF FKAIHDLMMF SGPQILKLLI KFVNDTKAPD 370 380 390 400 410 420 WQGYFYTVLL FVTACLQTLV LHQYFHICFV SGMRIKTAVI GAVYRKALVI TNSARKSSTV 430 440 450 460 470 480 GEIVNLMSVD AQRFMDLATY INMIWSAPLQ VILALYLLWL NLGPSVLAGV AVMVLMVPVN 490 500 510 520 530 540 AVMAMKTKTY QVAHMKSKDN RIKLMNEILN GIKVLKLYAW ELAFKDKVLA IRQEELKVLK 550 560 570 580 590 600 KSAYLSAVGT FTWVCTPFLV ALCTFAVYVT IDENNILDAQ TAFVSLALFN ILRFPLNILP 610 620 630 640 650 660 MVISSIVQAS VSLKRLRIFL SHEELEPDSI ERRPVKDGGG TNSITVRNAT FTWARSDPPT 670 680 690 700 710 720 LNGITFSIPE GALVAVVGQV GCGKSSLLSA LLAEMDKVEG HVAIKGVNLS GGQKQRVSLA 730 740 750 760 770 780 RAVYSNADIY LFDDPLSAVD AHVGKHIFEN VIGPKGMLKN KTRILVTHSM SYLPQVDVII 790 800 810 820 830 840 VMSGGKISEM GSYQELLARD GAFAEFLRTY ASTEQEQDAE ENGVTGVSGP GKEAKQMENG 850 860 870 880 890 900 MLVTDSAGKQ LQRQLSSSSS YSGDISRHHN STAELQKAEA KKEETWKLME ADKAQTGQVK 910 920 930 940 950 960 LSVYWDYMKA IGLFISFLSI FLFMCNHVSA LASNYWLSLW TDDPIVNGTQ EHTKVRLSVY 970 980 990 1000 1010 1020 GALGISQGIA VFGYSMAVSI GGILASRCLH VDLLHSILRS PMSFFERTPS GNLVNRFSKE 1030 1040 1050 1060 1070 1080 LDTVDSMIPE VIKMFMGSLF NVIGACIVIL LATPIAAIII PPLGLIYFFV QRFYVASSRQ 1090 1100 1110 1120 1130 1140 LKRLESVSRS PVYSHFNETL LGVSVIRAFE EQERFIHQSD LKVDENQKAY YPSIVANRWL 1150 1160 1170 1180 1190 1200 AVRLECVGNC IVLFAALFAV ISRHSLSAGL VGLSVSYSLQ VTTYLNWLVR MSSEMETNIV 1210 1220 1230 1240 1250 1260 AVERLKEYSE TEKEAPWQIQ ETAPPSSWPQ VGRVEFRNYC LRYREDLDFV LRHINVTING 1270 1280 1290 1300 1310 1320 GEKVGIVGRT GAGKSSLTLG LFRINESAEG EIIIDGINIA KIGLHDLRFK ITIIPQDPVL 1330 1340 1350 1360 1370 1380 FSGSLRMNLD PFSQYSDEEV WTSLELAHLK DFVSALPDKL DHECAEGGEN LRVIVLDKGE 1390 1400 IQEYGAPSDL LQQRGLFYSM AKDAGLV
	Isoform 7 (Delexon-18-30). Checksum: 371F14C07E53E200	FASTA	1,410	158,252
	10 20 30 40 50 60 MALRGFCSAD GSDPLWDWNV TWNTSNPDFT KCFQNTVLVW VPCFYLWACF PFYFLYLSRH 70 80 90 100 110 120 DRGYIQMTPL NKTKTALGFL LWIVCWADLF YSFWERSRGI FLAPVFLVSP TLLGITTLLA 130 140 150 160 170 180 TFLIQLERRK GVQSSGIMLT FWLVALVCAL AILRSKIMTA LKEDAQVDLF RDITFYVYFS 190 200 210 220 230 240 LLLIQLVLSC FSDRSPLFSE TIHDPNPCPE SSASFLSRIT FWWITGLIVR GYRQPLEGSD 250 260 270 280 290 300 LWSLNKEDTS EQVVPVLVKN WKKECAKTRK QPVKVVYSSK DPAQPKESSK VDANEEVEAL 310 320 330 340 350 360 IVKSPQKEWN PSLFKVLYKT FGPYFLMSFF FKAIHDLMMF SGPQILKLLI KFVNDTKAPD 370 380 390 400 410 420 WQGYFYTVLL FVTACLQTLV LHQYFHICFV SGMRIKTAVI GAVYRKALVI TNSARKSSTV 430 440 450 460 470 480 GEIVNLMSVD AQRFMDLATY INMIWSAPLQ VILALYLLWL NLGPSVLAGV AVMVLMVPVN 490 500 510 520 530 540 AVMAMKTKTY QVAHMKSKDN RIKLMNEILN GIKVLKLYAW ELAFKDKVLA IRQEELKVLK 550 560 570 580 590 600 KSAYLSAVGT FTWVCTPFLV ALCTFAVYVT IDENNILDAQ TAFVSLALFN ILRFPLNILP 610 620 630 640 650 660 MVISSIVQAS VSLKRLRIFL SHEELEPDSI ERRPVKDGGG TNSITVRNAT FTWARSDPPT 670 680 690 700 710 720 LNGITFSIPE GALVAVVGQV GCGKSSLLSA LLAEMDKVEG HVAIKGSVAY VPQQAWIQND 730 740 750 760 770 780 SLRENILFGC QLEEPYYRSV IQACALLPDL EILPSGDRTE IGEKTRILVT HSMSYLPQVD 790 800 810 820 830 840 VIIVMSGGKI SEMGSYQELL ARDGAFAEFL RTYASTEQEQ DAEENGVTGV SGPGKEAKQM 850 860 870 880 890 900 ENGMLVTDSA GKQLQRQLSS SSSYSGDISR HHNSTAELQK AEAKKEETWK LMEADKAQTG 910 920 930 940 950 960 QVKLSVYWDY MKAIGLFISF LSIFLFMCNH VSALASNYWL SLWTDDPIVN GTQEHTKVRL 970 980 990 1000 1010 1020 SVYGALGISQ GIAVFGYSMA VSIGGILASR CLHVDLLHSI LRSPMSFFER TPSGNLVNRF 1030 1040 1050 1060 1070 1080 SKELDTVDSM IPEVIKMFMG SLFNVIGACI VILLATPIAA IIIPPLGLIY FFVQRFYVAS 1090 1100 1110 1120 1130 1140 SRQLKRLESV SRSPVYSHFN ETLLGVSVIR AFEEQERFIH QSDLKVDENQ KAYYPSIVAN 1150 1160 1170 1180 1190 1200 RWLAVRLECV GNCIVLFAAL FAVISRHSLS AGLVGLSVSY SLQVTTYLNW LVRMSSEMET 1210 1220 1230 1240 1250 1260 NIVAVERLKE YSETEKEAPW QIQETAPPSS WPQVGRVEFR NYCLRYREDL DFVLRHINVT 1270 1280 1290 1300 1310 1320 INGGEKVGIV GRTGAGKSSL TLGLFRINES AEGEIIIDGI NIAKIGLHDL RFKITIIPQD 1330 1340 1350 1360 1370 1380 PVLFSGSLRM NLDPFSQYSD EEVWTSLELA HLKDFVSALP DKLDHECAEG GENLRVIVLD 1390 1400 1410 KGEIQEYGAP SDLLQQRGLF YSMAKDAGLV
	Isoform 8 (Delexon-17-18-30). Checksum: DF9B2914B8CAF4BD	FASTA	1,351	151,631
	10 20 30 40 50 60 MALRGFCSAD GSDPLWDWNV TWNTSNPDFT KCFQNTVLVW VPCFYLWACF PFYFLYLSRH 70 80 90 100 110 120 DRGYIQMTPL NKTKTALGFL LWIVCWADLF YSFWERSRGI FLAPVFLVSP TLLGITTLLA 130 140 150 160 170 180 TFLIQLERRK GVQSSGIMLT FWLVALVCAL AILRSKIMTA LKEDAQVDLF RDITFYVYFS 190 200 210 220 230 240 LLLIQLVLSC FSDRSPLFSE TIHDPNPCPE SSASFLSRIT FWWITGLIVR GYRQPLEGSD 250 260 270 280 290 300 LWSLNKEDTS EQVVPVLVKN WKKECAKTRK QPVKVVYSSK DPAQPKESSK VDANEEVEAL 310 320 330 340 350 360 IVKSPQKEWN PSLFKVLYKT FGPYFLMSFF FKAIHDLMMF SGPQILKLLI KFVNDTKAPD 370 380 390 400 410 420 WQGYFYTVLL FVTACLQTLV LHQYFHICFV SGMRIKTAVI GAVYRKALVI TNSARKSSTV 430 440 450 460 470 480 GEIVNLMSVD AQRFMDLATY INMIWSAPLQ VILALYLLWL NLGPSVLAGV AVMVLMVPVN 490 500 510 520 530 540 AVMAMKTKTY QVAHMKSKDN RIKLMNEILN GIKVLKLYAW ELAFKDKVLA IRQEELKVLK 550 560 570 580 590 600 KSAYLSAVGT FTWVCTPFLV ALCTFAVYVT IDENNILDAQ TAFVSLALFN ILRFPLNILP 610 620 630 640 650 660 MVISSIVQAS VSLKRLRIFL SHEELEPDSI ERRPVKDGGG TNSITVRNAT FTWARSDPPT 670 680 690 700 710 720 LNGITFSIPE GALVAVVGQV GCGKSSLLSA LLAEMDKVEG HVAIKTRILV THSMSYLPQV 730 740 750 760 770 780 DVIIVMSGGK ISEMGSYQEL LARDGAFAEF LRTYASTEQE QDAEENGVTG VSGPGKEAKQ 790 800 810 820 830 840 MENGMLVTDS AGKQLQRQLS SSSSYSGDIS RHHNSTAELQ KAEAKKEETW KLMEADKAQT 850 860 870 880 890 900 GQVKLSVYWD YMKAIGLFIS FLSIFLFMCN HVSALASNYW LSLWTDDPIV NGTQEHTKVR 910 920 930 940 950 960 LSVYGALGIS QGIAVFGYSM AVSIGGILAS RCLHVDLLHS ILRSPMSFFE RTPSGNLVNR 970 980 990 1000 1010 1020 FSKELDTVDS MIPEVIKMFM GSLFNVIGAC IVILLATPIA AIIIPPLGLI YFFVQRFYVA 1030 1040 1050 1060 1070 1080 SSRQLKRLES VSRSPVYSHF NETLLGVSVI RAFEEQERFI HQSDLKVDEN QKAYYPSIVA 1090 1100 1110 1120 1130 1140 NRWLAVRLEC VGNCIVLFAA LFAVISRHSL SAGLVGLSVS YSLQVTTYLN WLVRMSSEME 1150 1160 1170 1180 1190 1200 TNIVAVERLK EYSETEKEAP WQIQETAPPS SWPQVGRVEF RNYCLRYRED LDFVLRHINV 1210 1220 1230 1240 1250 1260 TINGGEKVGI VGRTGAGKSS LTLGLFRINE SAEGEIIIDG INIAKIGLHD LRFKITIIPQ 1270 1280 1290 1300 1310 1320 DPVLFSGSLR MNLDPFSQYS DEEVWTSLEL AHLKDFVSAL PDKLDHECAE GGENLRVIVL 1330 1340 1350 DKGEIQEYGA PSDLLQQRGL FYSMAKDAGL V
	Isoform 9. Checksum: D4CDAB576C03B431	FASTA	1,541	172,610
	10 20 30 40 50 60 MALRGFCSAD GSDPLWDWNV TWNTSNPDFT KCFQNTVLVW VPCFYLWACF PFYFLYLSRH 70 80 90 100 110 120 DRGYIQMTPL NKTKTALGFL LWIVCWADLF YSFWERSRGI FLAPVFLVSP TLLGITTLLA 130 140 150 160 170 180 TFLIQLERRK GVQSSGIMLT FWLVALVCAL AILRSKIMTA LKEDAQVDLF RDITFYVYFS 190 200 210 220 230 240 LLLIQLVLSC FSDRSPLFSE TIHDPNPCPE SSASFLSRIT FWWITGLIVR GYRQPLEGSD 250 260 270 280 290 300 LWSLNKEDTS EQVVPVLVKN WKKECAKTRK QPVKVVYSSK DPAQPKESSK VDANEEVEAL 310 320 330 340 350 360 IVKSPQKEWN PSLFKVLYKT FGPYFLMSFF FKAIHDLMMF SGPQILKLLI KFVNDTKAPD 370 380 390 400 410 420 WQGYFYTVLL FVTACLQTLV LHQYFHICFV SGMRIKTAVI GAVYRKALVI TNSARKSSTV 430 440 450 460 470 480 GEIVNLMSVD AQRFMDLATY INMIWSAPLQ VILALYLLWL NLGPSVLAGV AVMVLMVPVN 490 500 510 520 530 540 AVMAMKTKTY QVAHMKSKDN RIKLMNEILN GIKVLKLYAW ELAFKDKVLA IRQEELKVLK 550 560 570 580 590 600 KSAYLSAVGT FTWVCTPFLV ALCTFAVYVT IDENNILDAQ TAFVSLALFN ILRFPLNILP 610 620 630 640 650 660 MVISSIVQAS VSLKRLRIFL SHEELEPDSI ERRPVKDGGG TNSITVRNAT FTWARSDPPT 670 680 690 700 710 720 LNGITFSIPE GALVAVVGQV GCGKSSLLSA LLAEMDKVEG HVAIKGSVAY VPQQAWIQND 730 740 750 760 770 780 SLRENILFGC QLEEPYYRSV IQACALLPDL EILPSGDRTE IGEKGVNLSG GQKQRVSLAR 790 800 810 820 830 840 AVYSNADIYL FDDPLSAVDA HVGKHIFENV IGPKGMLKNK TRILVTHSMS YLPQVDVIIV 850 860 870 880 890 900 MSGGKISEMG SYQELLARDG AFAEFLRTYA STEQEQDAEE NGSTVMDEEE AGVTGVSGPG 910 920 930 940 950 960 KEAKQMENGM LVTDSAGKQL QRQLSSSSSY SGDISRHHNS TAELQKAEAK KEETWKLMEA 970 980 990 1000 1010 1020 DKAQTGQVKL SVYWDYMKAI GLFISFLSIF LFMCNHVSAL ASNYWLSLWT DDPIVNGTQE 1030 1040 1050 1060 1070 1080 HTKVRLSVYG ALGISQGIAV FGYSMAVSIG GILASRCLHV DLLHSILRSP MSFFERTPSG 1090 1100 1110 1120 1130 1140 NLVNRFSKEL DTVDSMIPEV IKMFMGSLFN VIGACIVILL ATPIAAIIIP PLGLIYFFVQ 1150 1160 1170 1180 1190 1200 RFYVASSRQL KRLESVSRSP VYSHFNETLL GVSVIRAFEE QERFIHQSDL KVDENQKAYY 1210 1220 1230 1240 1250 1260 PSIVANRWLA VRLECVGNCI VLFAALFAVI SRHSLSAGLV GLSVSYSLQV TTYLNWLVRM 1270 1280 1290 1300 1310 1320 SSEMETNIVA VERLKEYSET EKEAPWQIQE TAPPSSWPQV GRVEFRNYCL RYREDLDFVL 1330 1340 1350 1360 1370 1380 RHINVTINGG EKVGIVGRTG AGKSSLTLGL FRINESAEGE IIIDGINIAK IGLHDLRFKI 1390 1400 1410 1420 1430 1440 TIIPQDPVLF SGSLRMNLDP FSQYSDEEVW TSLELAHLKD FVSALPDKLD HECAEGGENL 1450 1460 1470 1480 1490 1500 SVGQRQLVCL ARALLRKTKI LVLDEATAAV DLETDDLIQS TIRTQFEDCT VLTIAHRLNT 1510 1520 1530 1540 IMDYTRVIVL DKGEIQEYGA PSDLLQQRGL FYSMAKDAGL V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line." Cole S.P.C., Bhardwaj G., Gerlach J.H., Mackie J.E., Grant C.E., Almquist K.C., Stewart A.J., Kurz E.U., Duncan A.M.V., Deeley R.G. Science 258:1650-1654(1992) [PubMed: 1360704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Multidrug resistance-associated protein: sequence correction." Cole S.P.C., Deeley R.G. Science 260:879-879(1993) [PubMed: 8098549] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Analysis of the intron-exon organization of the human multidrug-resistance protein gene (MRP) and alternative splicing of its mRNA." Grant C.E., Kurz E.U., Cole S.P.C., Deeley R.G. Genomics 45:368-378(1997) [PubMed: 9344662] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
[4]	NIEHS SNPs program Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-671; GLN-723; THR-861; SER-1047 AND ILE-1146.
[5]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 103-1531 (ISOFORM 9), VARIANT MET-117. Tissue: Brain.
[7]	"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q." Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D. Genomics 60:295-308(1999) [PubMed: 10493829] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 516-1531.
[8]	"Membrane topology of the multidrug resistance protein (MRP). A study of glycosylation-site mutants reveals an extracytosolic NH2 terminus." Hipfner D.R., Almquist K.C., Leslie E.M., Gerlach J.H., Grant C.E., Deeley R.G., Cole S.P.C. J. Biol. Chem. 272:23623-23630(1997) [PubMed: 9295302] [Abstract] Cited for: TOPOLOGY, GLYCOSYLATION AT ASN-19; ASN-23 AND ASN-1006.
[9]	"Topology mapping of the amino-terminal half of multidrug resistance-associated protein by epitope insertion and immunofluorescence." Kast C., Gros P. J. Biol. Chem. 272:26479-26487(1997) [PubMed: 9334225] [Abstract] Cited for: TOPOLOGY.
[10]	"Epitope insertion favors a six transmembrane domain model for the carboxy-terminal portion of the multidrug resistance-associated protein." Kast C., Gros P. Biochemistry 37:2305-2313(1998) [PubMed: 9485377] [Abstract] Cited for: TOPOLOGY.
[11]	"Characterization of a leukotriene C4 export mechanism in human platelets: possible involvement of multidrug resistance-associated protein 1." Sjoelinder M., Tornhamre S., Claesson H.-E., Hydman J., Lindgren J.A. J. Lipid Res. 40:439-446(1999) [PubMed: 10064732] [Abstract] Cited for: FUNCTION.
[12]	"The leukotriene C(4) transporter MRP1 regulates CCL19 (MIP-3beta, ELC)-dependent mobilization of dendritic cells to lymph nodes." Robbiani D.F., Finch R.A., Jaeger D., Muller W.A., Sartorelli A.C., Randolph G.J. Cell 103:757-768(2000) [PubMed: 11114332] [Abstract] Cited for: FUNCTION.
[13]	"Mutations of the Walker B motif in the first nucleotide binding domain of multidrug resistance protein MRP1 prevent conformational maturation." Cui L., Hou Y.-X., Riordan J.R., Chang X.-B. Arch. Biochem. Biophys. 392:153-161(2001) [PubMed: 11469806] [Abstract] Cited for: MUTAGENESIS OF ASP-792; ASP-793; LYS-1333 AND 1454-ASP-GLU-1455.
[14]	"Mutation of a single conserved tryptophan in multidrug resistance protein 1 (MRP1/ABCC1) results in loss of drug resistance and selective loss of organic anion transport." Ito K., Olsen S.L., Qiu W., Deeley R.G., Cole S.P.C. J. Biol. Chem. 276:15616-15624(2001) [PubMed: 11278867] [Abstract] Cited for: MUTAGENESIS OF TRP-1246.
[15]	"Identification of an amino acid residue in multidrug resistance protein 1 critical for conferring resistance to anthracyclines." Zhang D.-W., Cole S.P.C., Deeley R.G. J. Biol. Chem. 276:13231-13239(2001) [PubMed: 11278596] [Abstract] Cited for: MUTAGENESIS OF GLU-1089.
[16]	"Mutational analysis of ionizable residues proximal to the cytoplasmic interface of membrane spanning domain 3 of the multidrug resistance protein, MRP1 (ABCC1): glutamate 1204 is important for both the expression and catalytic activity of the transporter." Situ D., Haimeur A., Conseil G., Sparks K.E., Zhang D.-W., Deeley R.G., Cole S.P.C. J. Biol. Chem. 279:38871-38880(2004) [PubMed: 15208328] [Abstract] Cited for: MUTAGENESIS OF ARG-1046; ASP-1084 AND ARG-1131.
[17]	"Transmembrane helix 11 of multidrug resistance protein 1 (MRP1/ABCC1): identification of polar amino acids important for substrate specificity and binding of ATP at nucleotide binding domain 1." Zhang D.-W., Nunoya K., Vasa M., Gu H.-M., Theis A., Cole S.P.C., Deeley R.G. Biochemistry 43:9413-9425(2004) [PubMed: 15260484] [Abstract] Cited for: MUTAGENESIS OF GLN-580; THR-581; SER-585; ASN-597; SER-604 AND SER-605.
[18]	"Functional importance of three basic residues clustered at the cytosolic interface of transmembrane helix 15 in the multidrug and organic anion transporter MRP1 (ABCC1)." Conseil G., Deeley R.G., Cole S.P. J. Biol. Chem. 281:43-50(2006) [PubMed: 16230346] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ARG-1138; LYS-1141 AND ARG-1142, SUBCELLULAR LOCATION.
[19]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-915, MASS SPECTROMETRY.
[20]	"Structure of the human multidrug resistance protein 1 nucleotide binding domain 1 bound to Mg2+/ATP reveals a non-productive catalytic site." Ramaen O., Leulliot N., Sizun C., Ulryck N., Pamlard O., Lallemand J.-Y., Tilbeurgh H., Jacquet E. J. Mol. Biol. 359:940-949(2006) [PubMed: 16697012] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 642-871 IN COMPLEX WITH MG-ATP.
[21]	"Mutations in a gene encoding an ABC transporter cause pseudoxanthoma elasticum." Le Saux O., Urban Z., Tschuch C., Csiszar K., Bacchelli B., Quaglino D., Pasquali-Ronchetti I., Pope F.M., Richards A., Terry S., Bercovitch L., de Paepe A., Boyd C.D. Nat. Genet. 25:223-227(2000) [PubMed: 10835642] [Abstract] Cited for: VARIANTS GLN-633 AND VAL-671.
[22]	"Pseudoxanthoma elasticum: mutations in the MRP6 gene encoding a transmembrane ATP-binding cassette (ABC) transporter." Ringpfeil F., Lebwohl M.G., Christiano A.M., Uitto J. Proc. Natl. Acad. Sci. U.S.A. 97:6001-6006(2000) [PubMed: 10811882] [Abstract] Cited for: VARIANT VAL-671.
[23]	"Identification of human multidrug resistance protein 1 (MRP1) mutations and characterization of a G671V substitution." Conrad S., Kauffmann H.-M., Ito K., Deeley R.G., Cole S.P.C., Schrenk D. J. Hum. Genet. 46:656-663(2001) [PubMed: 11721885] [Abstract] Cited for: VARIANT SER-433, CHARACTERIZATION OF VARIANT VAL-671.
[24]	"Identification of novel polymorphisms in the pM5 and MRP1 (ABCC1) genes at locus 16p13.1 and exclusion of both genes as responsible for pseudoxanthoma elasticum." Perdu J., Germain D.P. Hum. Mutat. 17:74-75(2001) [PubMed: 11139250] [Abstract] Cited for: VARIANTS MET-117 AND LEU-1512.
[25]	"Polymorphism of the ABC transporter genes, MDR1, MRP1 and MRP2/cMOAT, in healthy Japanese subjects." Ito S., Ieiri I., Tanabe M., Suzuki A., Higuchi S., Otsubo K. Pharmacogenetics 11:175-184(2001) [PubMed: 11266082] [Abstract] Cited for: VARIANTS SER-43; ILE-73; GLN-723 AND GLN-1058.
[26]	"DNA sequencing of a cytogenetically normal acute myeloid leukaemia genome." Ley T.J., Mardis E.R., Ding L., Fulton B., McLellan M.D., Chen K., Dooling D., Dunford-Shore B.H., McGrath S., Hickenbotham M., Cook L., Abbott R., Larson D.E., Koboldt D.C., Pohl C., Smith S., Hawkins A., Abbott S. , Locke D., Hillier L.W., Miner T., Fulton L., Magrini V., Wylie T., Glasscock J., Conyers J., Sander N., Shi X., Osborne J.R., Minx P., Gordon D., Chinwalla A., Zhao Y., Ries R.E., Payton J.E., Westervelt P., Tomasson M.H., Watson M., Baty J., Ivanovich J., Heath S., Shannon W.D., Nagarajan R., Walter M.J., Link D.C., Graubert T.A., DiPersio J.F., Wilson R.K. Nature 456:66-72(2008) [PubMed: 18987736] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-433.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

NIEHS-SNPs

Cross-references

Sequence databases

EMBLGenBankDDBJ

L05628 mRNA. Translation: AAB46616.1.
AF022853 , AF022827, AF022828, AF022829, AF022831, AF022833, AF022835, AF022837, AF022839, AF022841, AF022850, AF022849, AF022848, AF022847, AF022846, AF022845, AF022844, AF022843, AF022842, AF022852, AF022851, AF022840, AF022838, AF022836, AF022834, AF022832, AF022826, AF022825, AF022824, AF022830 Genomic DNA. Translation: AAB83979.1.
AF022853 , AF022824, AF022825, AF022826, AF022828, AF022830, AF022832, AF022834, AF022836, AF022838, AF022848, AF022847, AF022846, AF022845, AF022844, AF022843, AF022842, AF022841, AF022839, AF022852, AF022851, AF022850, AF022849, AF022837, AF022835, AF022833, AF022831, AF022829, AF022827 Genomic DNA. Translation: AAB83980.1.
AF022853 , AF022824, AF022825, AF022826, AF022827, AF022829, AF022831, AF022833, AF022835, AF022837, AF022847, AF022846, AF022845, AF022844, AF022843, AF022842, AF022841, AF022840, AF022838, AF022852, AF022851, AF022850, AF022849, AF022848, AF022836, AF022834, AF022832, AF022830, AF022828 Genomic DNA. Translation: AAB83981.1.
AF022853 , AF022824, AF022825, AF022826, AF022827, AF022828, AF022829, AF022830, AF022831, AF022832, AF022833, AF022834, AF022835, AF022836, AF022837, AF022838, AF022839, AF022840, AF022841, AF022842, AF022843, AF022844, AF022845, AF022846, AF022847, AF022848, AF022849, AF022850, AF022851 Genomic DNA. Translation: AAB83983.1.
EF419769 Genomic DNA. Translation: ABN79590.1.
AB209120 mRNA. Translation: BAD92357.1.
U91318 Genomic DNA. Translation: AAC15784.1.
AC003026 Genomic DNA. Translation: AAC05808.1.

IPI

IPI00008338.
IPI00014985.
IPI00216262.
IPI00216263.
IPI00216264.
IPI00216265.
IPI00216266.
IPI00216267.
IPI00719178.

PIR

DVHUAR. A44231.

RefSeq

NP_004987.2.

UniGene

Hs.709181

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CBZ	X-ray	1.50	A	642-871	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P33527.

Protein family/group databases

TCDB

3.A.1.208.8. ATP-binding cassette (ABC) superfamily.

PTM databases

PhosphoSite

P33527.

Proteomic databases

PRIDE

P33527.

Genome annotation databases

Ensembl

ENST00000345148; ENSP00000263014; ENSG00000103222; Homo sapiens. [Genome view]
ENST00000346370; ENSP00000263019; ENSG00000103222; Homo sapiens. [Genome view]
ENST00000349029; ENSP00000263016; ENSG00000103222; Homo sapiens. [Genome view]
ENST00000351154; ENSP00000263017; ENSG00000103222; Homo sapiens. [Genome view]
ENST00000399408; ENSP00000382340; ENSG00000103222; Homo sapiens. [Genome view]
ENST00000399410; ENSP00000382342; ENSG00000103222; Homo sapiens. [Genome view]

GeneID

4363.

KEGG

hsa:4363.

UCSC

uc010bvi.1. human.
uc010bvj.1. human.
uc010bvk.1. human.
uc010bvl.1. human.

Organism-specific databases

GeneCards

GC16P015950.

H-InvDB

HIX0012837.

HGNC

HGNC:51. ABCC1.

HPA

CAB016097.
HPA002380.

MIM

158343. gene.

PharmGKB

PA244.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P33527.

Enzyme and pathway databases

Reactome

REACT_15314. Hormone biosynthesis.

Gene expression databases

ArrayExpress

P33527.

Bgee

P33527.

CleanEx

HS_ABCC1.

Genevestigator

P33527.

GermOnline

ENSG00000103222. Homo sapiens.

Family and domain databases

InterPro

IPR001140. ABC_TM_transpt.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR003593. ATPase_AAA+_core.
IPR005292. Multidrug-R_assoc_MRP.
[Graphical view]

Pfam

PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]

ProDom

PD000006. ABC_transporter. 2 hits.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00382. AAA. 2 hits.
[Graphical view]

TIGRFAMs

TIGR00957. MRP_assoc_pro. 1 hit.

PROSITE

PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00694. Daunorubicin.
DB01016. Glibenclamide.
DB01032. Probenecid.
DB01232. Saquinavir.
DB01138. Sulfinpyrazone.

SOURCE

Search...

Entry information

Entry name

MRP1_HUMAN

Accession

Primary (citable) accession number: P33527
Secondary accession number(s): A3RJX2 , O14819, O43333, P78419, Q59GI9, Q9UQ97, Q9UQ99, Q9UQA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 1, 1997
Last modified:	October 13, 2009
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.