Reviewed, UniProtKB/Swiss-Prot P33515 (POLG_MCFA)

Names and origin

Protein names	Recommended name:     Genome polyprotein Cleaved into the following 10 chains:     1- Recommended name:             Protein C         Alternative name(s):             Core protein             Capsid protein     2- Recommended name:             Small envelope protein M         Alternative name(s):             Matrix protein     3- Recommended name:             Envelope protein E     4- Recommended name:             Non-structural protein 1                 Short name=NS1     5- Recommended name:             Non-structural protein 2A                 Short name=NS2A     6- Recommended name:             Flavivirin protease NS2B regulatory subunit     7- Recommended name:             Flavivirin protease NS3 catalytic subunit               EC=3.4.21.91     8- Recommended name:             Non-structural protein 4A                 Short name=NS4A     9- Recommended name:             Non-structural protein 4B                 Short name=NS4B     10- Recommended name:             RNA-directed RNA polymerase               EC=2.7.7.48         Alternative name(s):             NS5
Organism	Mosquito cell fusing agent (CFA flavivirus) [Complete proteome]
Taxonomic identifier	31658 [NCBI]
Taxonomic lineage	Viruses › ssRNA positive-strand viruses, no DNA stage › Flaviviridae › Flavivirus
Virus host	Aedes [TaxID: 7158]

Protein attributes

Sequence length	3341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The small proteins NS2A, NS4A and NS4B are hydrophobic, suggesting a possible membrane-related function. NS5 may play a role in the viral RNA replication. The NS2B/NS3 protease complex processes the viral polyprotein.
Catalytic activity	Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala. Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	NS3 and NS2B form a heterodimer. NS3 is the catalytic subunit, whereas NS2B strongly stimulates the latter By similarity.
Subcellular location	Protein C: Virion Potential. Host membrane; Single-pass membrane protein Potential. Small envelope protein M: Virion Potential. Host membrane; Single-pass membrane protein Potential. Envelope protein E: Virion Potential. Host membrane; Multi-pass membrane protein Potential. Non-structural protein 4A: Host membrane; Single-pass membrane protein Potential. Non-structural protein 4B: Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins By similarity. In CFA flavivirus the processing of prM to M either does not occur or occurs very inefficiently.
Miscellaneous	The virion of this virus is a nucleocapsid covered by a lipoprotein envelope. The envelope contains two proteins: the protein M and glycoprotein E. The nucleocapsid is a complex of protein C and mRNA. In immature particles, there are 60 icosaedrally organized trimeric spikes on the surface. Each spike consists of three heterodimers of envelope protein M precursor (prM) and envelope protein E By similarity.
Sequence similarities	Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 peptidase S7 domain. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Capsid protein Core protein Envelope protein Membrane Virion
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
PTM	Cleavage on pair of basic residues Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	methylation Inferred from electronic annotation. Source: InterPro transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW viral nucleocapsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: InterPro RNA helicase activity Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 136	136	Protein C Potential		PRO_0000037714
	Chain	137 – 278	142	Small envelope protein M Potential		PRO_0000037715
	Chain	279 – 705	427	Envelope protein E Potential		PRO_0000037716
	Chain	706 – 1095	390	Non-structural protein 1 Potential		PRO_0000037717
	Chain	1096 – 1327	232	Non-structural protein 2A Potential		PRO_0000037718
	Chain	1328 – 1451	124	Flavivirin protease NS2B regulatory subunit Potential		PRO_0000037719
	Chain	1452 – 2028	577	Flavivirin protease NS3 catalytic subunit Potential		PRO_0000037720
	Chain	2029 – 2196	168	Non-structural protein 4A Potential		PRO_0000037721
	Chain	2197 – 2454	258	Non-structural protein 4B Potential		PRO_0000037722
	Chain	2455 – 3341	887	RNA-directed RNA polymerase Potential		PRO_0000037723
Regions
	Transmembrane	245 – 261	17	Potential
	Transmembrane	670 – 686	17	Potential
	Domain	1458 – 1624	167	Peptidase S7
	Domain	1627 – 1780	154	Helicase ATP-binding
	Domain	1793 – 1947	155	Helicase C-terminal
	Domain	2970 – 3117	148	RdRp catalytic
	Nucleotide binding	1640 – 1647	8	ATP Potential
	Region	371 – 384	14	Involved in fusion Potential
	Motif	1729 – 1732	4	DECH box
Sites
	Active site	1506	1	Charge relay system By similarity
	Active site	1530	1	Charge relay system By similarity
	Active site	1589	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	157	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	243	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	339	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	399	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	411	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	575	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	611	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	794	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	896	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	993	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	1027	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	P33515-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: BF0715F836F245ED	FASTA	3,341	373,267
	10 20 30 40 50 60 MKRKDLEARG KAPGRDSSTP FWGREGRRKD KDKGGESPSN RQVTLKTPIQ SGRRAGKRQR 70 80 90 100 110 120 VGLLGRLGVG WGSFLQEDIV QALIHMALVL HALFASIDRR IRSLSRRVTA LESRRTTGNP 130 140 150 160 170 180 MTLAFILGFL TVLCGCVVID MQVSTTRGTE IFEGETNRTD YLHLLKLPAD GCWSGILVTK 190 200 210 220 230 240 KCPKVTDLAK DLESTDCGST WTEFTLRYRR CVVKKREKRS REPPKADLLA EMEIIAFKTI 250 260 270 280 290 300 RENKTIFIVA LLCVAIAKRW PTWVVILLAI GTWTTVKGEF VEPLYTLKAE QMTMLQTIVR 310 320 330 340 350 360 PEEGYVVATP NGLLEFKTGP AEIYGGQWLR ELLADCHVNA SYSTDVCPGG SQLNMADIMA 370 380 390 400 410 420 KERVCSTQPY NRGWGTGCFK WGIGFVGTCV ELHCDRGFNV SSIARSAIVM NVTASFHSVS 430 440 450 460 470 480 DTQQMVGDIP LTFRFAKLGN AAMTCRLESE QLLLDYYHVT GSSHEGLFLR SQVDSWPGVH 490 500 510 520 530 540 STASGRHGME KVVVWGDARS NEILVKNVIE PSLSWEDAIA THGGFRDISF VCQIMLDKLV 550 560 570 580 590 600 SGAFRDCPGP KISTFSQDGF GYSGVVITTL TASSNETCSL SLTCHGCLLQ STKMIFLAGK 610 620 630 640 650 660 TTSRAFVKCG NHTSTLLVGS TSVSIECALN PISQGWRLAR HVVDRYRRFG VSGVAGVWQD 670 680 690 700 710 720 LVGKFSVGAF FSNTALLVIL VLAALIDKRI AFLLVLGGYF YYVRADLGCG IDTTRKTISC 730 740 750 760 770 780 GSGVFVWKHL GVGISNDHAV ELEDYSFTDL YIKDMFSWTT KPCLICEDAL QCVALRRAAF 790 800 810 820 830 840 SAVGSMGSER VYVNDTLART FKFSETAKRT ISVTINLIQY KFSSYVAHGR AEGDLGLLPT 850 860 870 880 890 900 MYGSYPEKEA DKVIRIVASR PDIRRLCGKA VSFQFKFTGF RRGLYGSNVQ VEVSKNSSTE 910 920 930 940 950 960 CPTYLAGVAV KNGRTVITDG MFWMESIVLD GVAQITSLEM RQSHRCVWPR EYTPDTLSDP 970 980 990 1000 1010 1020 SDQALFIPPA WGGPISRVNH IIGYKTQTDF PWNVSDITLI EGPAPGTKVK VDSRCHGRMH 1030 1040 1050 1060 1070 1080 AQVIGPNDTE SWCCQSCTRI VHFRVGDLLY YPMEIQLGTM SEASEPNSKI FEEPIGEEPE 1090 1100 1110 1120 1130 1140 PTVDDILKRY GKANAQSDFR RVSQRAGVWF DRSLLNLLCL AISLQLIGAK TRTSTLTRLF 1150 1160 1170 1180 1190 1200 LTILAMALFG LPNLFSSVGL SAWVLLVASS SAQPQDLSMN LWIVLQTGSS AVLLLGYMIR 1210 1220 1230 1240 1250 1260 RKLAMVLGVH HLVTLMCVQF LFSAVDRYQK YLYGLLELMA SVVLLSAYKS VLQALPPEVL 1270 1280 1290 1300 1310 1320 CFSLVMGWKT ALSLATVVFL IFSLNAMYKY ACQYHNPRNG YRDSGANLWF WTVSLASAGG 1330 1340 1350 1360 1370 1380 IWAAEKAHQP TVAAVLAFTM VVLFLYMEQT NVSMELEFIS AGETPEGVST ENDDGINIPD 1390 1400 1410 1420 1430 1440 LKGRYGEDGI VVGAASSSGY LPELVFVFLL GFAVTSTSYF LGALYLLIAT STNLPVVIIR 1450 1460 1470 1480 1490 1500 MLRMKLTASN RSDDLLGLGG PVETDLQTSF QDIPNGVYRI VVRSLFGDRQ RGAGFSKNGV 1510 1520 1530 1540 1550 1560 FHTLMHVTRG EPVKWRGRVV VPHSGSALRD VVSYGGPWQL DTPTTTEDLV LMACKPDKTI 1570 1580 1590 1600 1610 1620 EYHRYRPGVM SIDGEPVMFI SDDFGKGSSG SPFFINGEPV GFYGFGFYVN GIYRSTVAGG 1630 1640 1650 1660 1670 1680 KPTDVTESLN CDSTRRFVTW HPGKGKTRKV IVEETKKNYD SNQRTVILTP TRVVMAEVVE 1690 1700 1710 1720 1730 1740 ALNNSGMRSD KNLSYCTRNL ITVACHATFT KFVLSHGAKK VRVAMIIMDE CHFMDPMSIA 1750 1760 1770 1780 1790 1800 ARGILEHLHG QGTKLIYLSA TPPGHAPDTG SNYAISDQSI SFPTWLSPAW IGNVQKSVGA 1810 1820 1830 1840 1850 1860 KKTILFVPSH NQANTLASAI PGSVPLHRAN FSSNYAQAGD AATALVISTD ISEMGANLGV 1870 1880 1890 1900 1910 1920 DLVIDTRRAL RPLVDSATRV KLVETNITTS SMIQRRGRTG RREPGTYVYP IDSQTEENPV 1930 1940 1950 1960 1970 1980 SWVCWPEAQM ILDQLGMTFM LEEAAYSQPP GRFTLVGEDR MRFLKLMDRD DIPIWLAWHW 1990 2000 2010 2020 2030 2040 AEAGDRRHSA LFQGAGTGKI IENRFGKQEY RPQYVDDRFE SIEWETRKVS IDFYMNCRGG 2050 2060 2070 2080 2090 2100 PTLYEFFTVV DWTDIWRRTA SALWDLSDVM NGEVRDRYTT ERSLTVVMAF VLGVSIMLSC 2110 2120 2130 2140 2150 2160 FIAVWALCFL FSLFRPKKAT YEQMPSSDPL SGGVLVSTPS VLYCMGVPLG FCVVITLAMF 2170 2180 2190 2200 2210 2220 LVYPVLYKSI GNRSYMDSDL VKWVILGSCL ICGVLAWEMR MFPNIRSDLM ELVKAVKEPE 2230 2240 2250 2260 2270 2280 EVVNSGPSFP SWEIAQGKGA TMLDSLQVFF FITVLSTKFL YWFQENWTAR MYAMKHPEMV 2290 2300 2310 2320 2330 2340 SSIGGFRFDE IPFRAVLPSG FAIVAIASLP SVVVGLLAAG VFMAIMYCQN KWNATPKILT 2350 2360 2370 2380 2390 2400 ALDARDQRHD RPTEITSRVP LENTRSIMYA FCLIFSLFWA FCTRSPGDFL RGSLVVGASM 2410 2420 2430 2440 2450 2460 WQILHPRSKI HDVMDFGSMV SAIGLLEMNY LFYRFMHIAA RALGAVAPFN QFRALEKSTT 2470 2480 2490 2500 2510 2520 IGLGMKWKMT LNALDGDAFT RYKSRGVNET ERGDYVSRGG LKLNEIISKY EWRPSGRVVD 2530 2540 2550 2560 2570 2580 LGCGRGGWSQ RAVMEETVSS ALGFTIGGAE KENPQRFVTK GYNLATLKTG VDVHRLTPFR 2590 2600 2610 2620 2630 2640 CDTIMCDIGE SDPSPIKEKT RTLKVLQLLE NWLLVNPGAH FVCKILSPYS LEVLRKIESL 2650 2660 2670 2680 2690 2700 QHLYNGRLVR LSHSRNSSVE MYYISGARSN VVRTTYMTLA ALMARFSRHL DSVVLPSPVL 2710 2720 2730 2740 2750 2760 PKGTRADPAA SVASMNTSDM MDRVERLMNE NRGTWFEDQQ HPYKSFKYFG SFVTDDVKVG 2770 2780 2790 2800 2810 2820 GQAVNPLVRK IMWPWETLTS VVGFSMTDVS TYSQQKVLRE KVDTVIPPHP QHIRRVNRTI 2830 2840 2850 2860 2870 2880 TKHFIRLFKN RNLRPRILSK EEFVANVRND AAVGSWSRDV PWRDVQEAIQ DQCFWDLVGK 2890 2900 2910 2920 2930 2940 ERALHLQGKC EMCIYNTMGK KEKKPSLAGE AKGSRTIWYM WLGSRFLEFE ALGFLNADHW 2950 2960 2970 2980 2990 3000 VSREHFPGGV GGVGVNYFGY YLKDIASRGK YLIADDIAGW DTKISEEDLE DEEALLTALT 3010 3020 3030 3040 3050 3060 EDPYHRALMA ATMRLAYQNI VAMFPRTHSK YGSGTVMDVV GRRDQRGSGQ VVTYALNTIT 3070 3080 3090 3100 3110 3120 NGKVQVARVL ESEGLLQADE SVLDAWLEKH LEEALGNMVI AGDDVVVSTD NRDFSSALEY 3130 3140 3150 3160 3170 3180 LELTGKTRKN VPQGAPSRME SNWEKVEFCS HHYHEMSLKD GRIIIAPCRH ENEVLGRSRL 3190 3200 3210 3220 3230 3240 QKGGVVSISE SACMAKAYAQ MWALYYFHRR DLRLGFIAIS SAVPTNWFPL GRTSWSVHQY 3250 3260 3270 3280 3290 3300 HEWMTTDDML RVWNDVWVHN NPWMLNKESI ESWDDIPYLH KKQDITCGSL IGVKERATWA 3310 3320 3330 3340 REIENSVISV RRIIDAETGV LNTYKDELSV MSRYRRGNDV I

References

[1]

"The complete nucleotide sequence of cell fusing agent (CFA): homology between the nonstructural proteins encoded by CFA and the nonstructural proteins encoded by arthropod-borne flaviviruses." Cammisa-Parks H., Cisar L.A., Kane A., Stollar V. Virology 189:511-524(1992) [PubMed: 1322586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M91671 Genomic RNA. Translation: AAA48509.1.
PIR	A42996.
RefSeq	NP_041725.1.
3D structure databases
HSSP	HSSP built from PDB template 1L9K based on UniProtKB Q88653.
ModBase	Search...
Protein family/group databases
MEROPS	S07.001.
Genome annotation databases
GeneID	1496976.
Family and domain databases
InterPro	IPR014001. DEAD-like_N. IPR011492. DEAD_Flavivir. IPR001650. DNA/RNA_helicase_C. IPR013755. Flav_glyE_cen_1. IPR001157. Flavi_NS1. IPR014412. Gen_Poly_FLV. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR001850. Peptidase_S7. IPR000208. RNA_pol_flaviviral. IPR007094. RNA_pol_PSvir. IPR002877. rRNA_MeTrfase_RrmJ/FtsJ. [Graphical view]
Gene3D	G3DSA:3.30.387.10. Flav_glyE_cen_1. 1 hit.
Pfam	PF07652. Flavi_DEAD. 1 hit. PF00948. Flavi_NS1. 1 hit. PF00972. Flavi_NS5. 1 hit. PF01728. FtsJ. 1 hit. PF00271. Helicase_C. 1 hit. PF00949. Peptidase_S7. 1 hit. [Graphical view]
PIRSF	PIRSF003817. Gen_Poly_FLV. 1 hit.
ProDom	PD001496. Flavi_NS1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00487. DEXDc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POLG_MCFA

Accession

Primary (citable) accession number: P33515

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)