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Protein

Auxin-binding protein 1

Gene

ERABP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Auxin receptor that controls cell elongation and cell division (PubMed:9804548, PubMed:11297513). Involved in embryonic morphogenesis (PubMed:11297513). Acts on the cell cycle, endocycle, cell plate formation, and cell expansion and contributes to the control of auxin-related gene expression (PubMed:18952781). Controls root meristem size and mediates auxin responsiveness (PubMed:19777056). Involved in activation of ROP GTPases in response to auxin and regulation of clathrin-mediated endocytosis in roots (PubMed:20887895, PubMed:22683261). Acts as a positive factor in clathrin recruitment to the plasma membrane, thereby promoting endocytosis (PubMed:20887896, PubMed:25922490). Upon auxin binding, restricts the internalization of PIN proteins by inhibiting clathrin-mediated endocytosis (PubMed:20887896, PubMed:25922490). Involved in the regulation of polar auxin transport (PubMed:21223392). Behaves as a negative regulator of the SCF(TIR1/AFB) signaling pathway, protecting AUX/IAA repressors from degradation (PubMed:24051655). Regulates the expression of cell wall remodeling genes via an SCF(TIR1/AFB)-dependent pathway (PubMed:24424095). Involved in the modulation of hemicellulose xyloglucan structure (PubMed:24424095). Required for rapid auxin-mediated re-orientation of microtubules to regulate cell elongation in roots and dark-grown hypocotyls as well as asymmetric growth during gravitropic responses (PubMed:25409144). Involved in the shade avoidance response (PubMed:24052532). Forms with TMK1 a cell surface auxin perception complex that activates ROP signaling pathways (PubMed:24578577). ABP1 sensing of auxin is important for the ABP1-TMK1 complex formation (PubMed:24578577). Interacts functionally with phytochrome to regulate growth (PubMed:25392478).15 Publications

Caution

Analysis of new null mutants leads to the conclusion that plants do not need ABP1 for auxin signaling and for their growth and development under normal growth conditions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei81Auxin bindingBy similarity1
Metal bindingi92Zinc; via tele nitrogenBy similarity1
Metal bindingi94Zinc; via tele nitrogenBy similarity1
Metal bindingi98ZincBy similarity1
Metal bindingi141Zinc; via tele nitrogenBy similarity1

GO - Molecular functioni

  • auxin binding Source: TAIR
  • receptor activity Source: InterPro
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • cytokinesis by cell plate formation Source: TAIR
  • positive regulation of cell division Source: TAIR
  • positive regulation of cell size Source: TAIR
  • positive regulation of DNA endoreduplication Source: TAIR
  • unidimensional cell growth Source: TAIR

Keywordsi

Molecular functionReceptor
Biological processAuxin signaling pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Auxin-binding protein 11 Publication
Short name:
ABP11 Publication
Gene namesi
Name:ERABP11 Publication
Ordered Locus Names:At4g02980Imported
ORF Names:T4I9.14Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G02980
TAIRilocus:2005491 AT4G02980

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryo lethality, when homozygous (PubMed:11297513). No visible phenotype was found for other null mutants (PubMed:25646447).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57R → K: Slight reduction of activation by auxin. Slight reduction of activation by auxin; when associated with V-60. 1 Publication1
Mutagenesisi60L → V: Slight reduction of activation by auxin. Slight reduction of activation by auxin; when associated with K-57. 1 Publication1
Mutagenesisi60L → Y in abp1-9; lower auxin sensitivity and altered responses to continuous light and shade. 1 Publication1
Mutagenesisi81Q → D: Slight reduction of activation by auxin. 1 Publication1
Mutagenesisi89T → I in abp1-8; lower auxin sensitivity and altered responses to continuous light and shade. 1 Publication1
Mutagenesisi89T → V: Slight reduction of activation by auxin. Slight reduction of activation by auxin; when associated with L-90. 1 Publication1
Mutagenesisi90P → L: Slight reduction of activation by auxin. Slight reduction of activation by auxin; when associated with V-89. 1 Publication1
Mutagenesisi92H → A in ABP-M1X; Strong reduction of activation by auxin. In ABP-M2X; strong reduction of activation by auxin; when associated with A-94. 1 Publication1
Mutagenesisi92H → Y in abp1-5; defects in pavement cells interdigitation and decreased interaction with TMK1. 2 Publications1
Mutagenesisi94H → A in ABP-M2X; strong reduction of activation by auxin; when associated with A-92. 1 Publication1
Mutagenesisi99V → A: No effect on activation by auxin. 1 Publication1
Mutagenesisi125F → L: No effect on activation by auxin. 1 Publication1
Mutagenesisi136P → L: No effect on activation by auxin. 1 Publication1
Mutagenesisi141H → N in abp1-10; lower auxin sensitivity and altered responses to continuous light and shade. 1 Publication1
Mutagenesisi181F → L: Slight reduction of activation by auxin. Slight reduction of activation by auxin; when associated with Y-185. 1 Publication1
Mutagenesisi185W → Y: Slight reduction of activation by auxin. Slight reduction of activation by auxin; when associated with L-181. 1 Publication1
Mutagenesisi188Q → D: No effect on activation by auxin. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 331 PublicationAdd BLAST33
ChainiPRO_000002061334 – 198Auxin-binding protein 1Add BLAST165

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 189By similarity
Glycosylationi130N-linked (GlcNAc...) asparagineBy similarity1

Post-translational modificationi

Glycosylated.By similarity
Ubiquitinated by RMA2, leading to proteasomal degradation.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiP33487

Expressioni

Inductioni

Up-regulated by auxin.1 Publication

Gene expression databases

ExpressionAtlasiP33487 baseline and differential
GenevisibleiP33487 AT

Interactioni

Subunit structurei

Homodimer (By similarity). May interact with the GPI-anchored plasma membrane protein SKU5 and its family members (PubMed:16649105). Interacts with TMK1 (via extracellular domain) (PubMed:24578577).1 PublicationBy similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TMK1P432983EBI-16094614,EBI-2023970

Protein-protein interaction databases

BioGridi13409, 1 interactor
DIPiDIP-61369N
IntActiP33487, 1 interactor
STRINGi3702.AT4G02980.1

Structurei

3D structure databases

ProteinModelPortaliP33487
SMRiP33487
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 60Auxin bindingBy similarity4
Regioni89 – 90Auxin bindingBy similarity2
Regioni183 – 185Auxin bindingBy similarity3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi195 – 198Prevents secretion from ER4

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IWVR Eukaryota
ENOG4112D1V LUCA
HOGENOMiHOG000030816
InParanoidiP33487
OMAiFPNSTFH
OrthoDBiEOG09360MCM
PhylomeDBiP33487

Family and domain databases

Gene3Di2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR000526 Auxin-bd
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR37236 PTHR37236, 1 hit
PfamiView protein in Pfam
PF02041 Auxin_BP, 1 hit
PRINTSiPR00655 AUXINBINDNGP
SUPFAMiSSF51182 SSF51182, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33487-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVLSVGSAS SSPIVVVFSV ALLLFYFSET SLGAPCPING LPIVRNISDL
60 70 80 90 100
PQDNYGRPGL SHMTVAGSVL HGMKEVEIWL QTFAPGSETP IHRHSCEEVF
110 120 130 140 150
VVLKGSGTLY LAETHGNFPG KPIEFPIFAN STIHIPINDA HQVKNTGHED
160 170 180 190
LQVLVIISRP PIKIFIYEDW FMPHTAARLK FPYYWDEQCI QESQKDEL
Length:198
Mass (Da):22,044
Last modified:February 1, 1994 - v1
Checksum:i43440CFDCE9EFD67
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102V → L in AAM64865 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55111 Genomic DNA Translation: CAA38909.1
X69901 Genomic DNA Translation: CAA49526.1
S40550 mRNA Translation: AAB22612.1
AF069442 Genomic DNA Translation: AAC79108.1
AL161495 Genomic DNA Translation: CAB77783.1
CP002687 Genomic DNA Translation: AEE82256.1
AF389278 mRNA Translation: AAK63851.1
AY093754 mRNA Translation: AAM10378.1
AY087315 mRNA Translation: AAM64865.1
PIRiS31584
RefSeqiNP_192207.1, NM_116532.3
UniGeneiAt.148

Genome annotation databases

EnsemblPlantsiAT4G02980.1; AT4G02980.1; AT4G02980
GeneIDi828120
GrameneiAT4G02980.1; AT4G02980.1; AT4G02980
KEGGiath:AT4G02980

Similar proteinsi

Entry informationi

Entry nameiABP1_ARATH
AccessioniPrimary (citable) accession number: P33487
Secondary accession number(s): Q8LBB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 25, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health